Human papilloma virus in the urethra in HIV-infected men in the Рrimorsk region

2021 ◽  
Vol 13 (1) ◽  
pp. 28-36
Author(s):  
O. M. Grigorian ◽  
L. F. Sklyar ◽  
O. V. Filipova, ◽  
E. V. Gavro ◽  
S. N. Beniova ◽  
...  
Keyword(s):  
Hpv Infection ◽  
Hiv Viral Load ◽  
Papilloma Virus ◽  
Face To Face ◽  
Anogenital Region ◽  
And Control ◽  
Hiv 1 ◽  
Hpv 18 ◽  
Immunodeficiency States

Objective of the study: identification of HPV and identification of the most common types in urethral swabs taken from HIV-1- infected men, as well as determination of the dependence of the presence of HPV in patients with HIV viral load (VL) and CD4 + T-lymphocyte count.Materials and methods. The study included 34 HIV-1-infected men being monitored at the Center for the Prevention and Control of AIDS and Infectious Diseases (Vladivostok). They were asked to undergo a urological examination, including the collection of urethral swabs, as well as a face-to-face interview to collect socio-demographic, behavioral and clinical-anamnestic data.Results. HIV-1-infected patients (34 people) included in the study were 25–60 (37,8±7,1) years old. HPV was detected in 23 (67,6%) patients aged 25–45 (37,8±5,5) years. The vast majority of patients with multiple HPV infection reported having had sex with three or more sexual partners and did not always use a condom, and 17.4% of the men surveyed said they never used one. In the past, 47.8% of HPV-positive patients used narcotic substances, and 30.4% of the respondents, at the time of the survey, were active users of psychoactive substances. In 23 HIV 1-infected men, 9 genotypes were identified — 6 (HPV-HP), 16, 18, 33, 35, 52, 53, 58, 73 (HPV-VR) — belonging to 5 types of HPV: AlphaPV-6, AlphaPV-7, AlphaPV-9, AlphaPV-10, AlphaPV-11. In 5 patients, coinfection of two genotypes was revealed, in 3 — two types of HPV. The most common genotype was HPV-16 (30,4%) and HPV-18 (26,1%), the least HPV-{6, 58, 73} (1/23≈4,3%). The presented results indicate the need to create diagnostic programs focused on early detection of cancer of the anogenital region in persons of both sexes in patients with immunodeficiency states, primarily in HIV 1-positive patients with HPV-HR.

scholarly journals Absence of HPV Infection Is Associated with Smoker Patients with Squamous Cell Carcinoma of the Oropharynx

2014 ◽  
Vol 2014 ◽  
pp. 1-5
Author(s):  
Gláucia Resende Soares ◽  
Adriana Demathé ◽  
Neivio José Mattar ◽  
Éder Ricardo Biasoli ◽  
Glauco Issamu Miyahara
Keyword(s):  
Polymerase Chain Reaction ◽  
Tobacco Consumption ◽  
Hpv Infection ◽  
Chain Reaction ◽  
Biopsy Tissue ◽  
Two Samples ◽  
Polymerase Chain ◽  
Hpv 18

The purpose of this study was to evaluate the survival of patients with SCC of the oropharynx, according to the presence of HPV and tobacco consumption. A total of 37 patients were followed up for at least 5 years after being diagnosed with SCC of the oropharynx. The biopsy tissue was submitted to the polymerase chain reaction (PCR) and in situ hybridization (ISH) methods for broad determination of HPV presence, to identify the presence of high-risk viruses (16 and 18). 12 of the 37 (32.4%) samples were HPV positive, whereas the two specific types of virus were identified in two samples for HPV-16 and in no samples for HPV-18. We observed no significant effect of the virus in survival analysis, irrespective of tobacco consumption. The level of tobacco consumption was significantly higher in the group of HPV-negative patients (P=0.0283), in which all the patients in this group were smokers. Therefore, HPV did not change the survival of patients with SCC of the oropharynx in this study, indicating that factors other than tobacco need to be studied in conjunction with it, and the level of tobacco consumption is significantly higher in the group of HPV-negative patients.

scholarly journals Noninfectious papilloma virus–like particles inhibit HIV-1 replication: implications for immune control of HIV-1 infection by IL-27

Blood ◽  
2006 ◽  
Vol 109 (5) ◽  
pp. 1841-1849 ◽  
Author(s):  
J. Mohamad Fakruddin ◽  
Richard A. Lempicki ◽  
Robert J. Gorelick ◽  
Jun Yang ◽  
Joseph W. Adelsberger ◽  
...  
Keyword(s):  
T Cells ◽  
Cd4 T Cells ◽  
Mononuclear Cells ◽  
Interleukin 10 ◽  
Hpv Infection ◽  
Interferon Α ◽  
Novel Therapy ◽  
Peripheral Blood Mononuclear ◽  
Papilloma Virus ◽  
Hiv 1

AbstractHuman papilloma virus (HPV)–like particles (VLPs) have been used as a vaccine to prevent HPV infection. Recent studies demonstrate that VLPs bind to dendritic cells and induce the expression of antiviral cytokines such as interferon-α (IFN-α), interleukin-10 (IL-10) and IFN-γ. In the present study, we evaluated the effect of VLPs on HIV-1 replication in peripheral blood mononuclear cells (PBMCs), CD4+ T cells, and macrophages. Here, we show that VLPs suppress the replication of both X4 and R5 HIV-1 without affecting the expression of CD4, CXCR4, and CCR5. Soluble factor(s) released by PBMCs and macrophages on VLPs treatment inhibited HIV-1 replication. To determine the inhibitory factors, DNA microarray analysis was performed using VLP-treated PBMCs and macrophages. VLPs induced the genes associated with IFN induction, immune responses, and antiviral responses, among with the recently described cytokine IL-27. Subsequently, IL-27 was found to be a potent inhibitor of HIV-1 replication in PBMCs, CD4+ T cells, and macrophages. Taken together, our studies identify a novel role of IL-27 in restricting HIV-1 replication and suggest that further examination of the inhibitory property of IL-27 may pave the way for a novel therapy for HIV-1 infection.

scholarly journals Seroprevalence of Human Papilloma Virus in Aborted Women in Kirkuk City

2018 ◽  
Vol 2 (2) ◽  
pp. 221-230
Author(s):  
Jacklen khoshaba ◽  
Salwa Mustafa ◽  
Muhannad Alazzawy
Keyword(s):  
Human Papilloma Virus ◽  
First Trimester ◽  
Hpv Infection ◽  
Cross Sectional Study ◽  
Control Group ◽  
Significant Relation ◽  
Cross Sectional ◽  
Papilloma Virus ◽  
And Control ◽  
Cytomegalovirus Infections

The aimed of the study was to evaluate the Seroprevalence of human papilloma virus (HPV) in aborted women. A cross-sectional study was carried out in Kirkuk city from 10th of March 2017 to 10th of July 2017 The number of aborted women understudy were 65 women who attended for curettage in Kirkuk general hospital. The control group who were matched to the patients studied included 30 normal pregnant women at the full term of pregnancy, women with Toxoplasma gondii , rubella and cytomegalovirus infections were excluded from the study. Five ml of blood was collected by vein puncture from each woman in the study, the obtained sera then organized to determine of HPV 16 E7 protein in patients and control using ELISA technique. The study showed that the highest rate of HPV infection (35.38%) was recorded among women with abortion comparing with the control group, with a highly significant relation. The study showed that 75.38% of women had aborted in the 1st trimester of pregnancy and the lowest rate of abortion 9.24% was in the 3rd trimester. The study showed that the highest rate of HPV infection was found in women with 1st trimester of abortion (38.78%) and the lowest rate was in the 3rd trimester of abortion. The study showed that the highest rate of HPV infection was recorded among aborted women who have suffered from one abortion during their marriage life. It was concluded that there was a significant relation of HPV with abortion spatially in women who were aborted in the first trimester of pregnancy.

scholarly journals Determination of papilloma-virus infection reproductive phase in algorithm of monitoring patients with cervical displasia

2021 ◽  
Vol 51 (4) ◽  
pp. 61-63
Author(s):  
S. A. Selkov ◽  
G. N. Vedeneeva ◽  
I. A. Baskakova ◽  
S. R. Baur
Keyword(s):  
Intraepithelial Neoplasia ◽  
Hpv Infection ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Hpv 16 ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Virus Activity ◽  
Polymerase Chain

HPV 16 and 18 are known to be the main cause of cervical intraepithelial neoplasia (CIN) and cervical cancer. The terms of HPV persistence in the host and, coordinately, the risk of cervical neoplasia development and progression are determined in much extent by virus activity. The purpose of this investigation was the detection of HPV DNA presence in cervical epithelium as well as confirmation of its activity by means of immunocytochemistry and reverse transcriptase polymerase chain reaction. The level of HPV inf ection by oncogenic and nononcogenic types in 181 women with different cervical pathology was 55,8%. The active stage of HPV infection was confirmed in 27,5% of HPV-inf ected women mainly with low grades of CIN. The proof of reproductive general HPV infection was more informative with RT PCR just as for HPV 16 and 18 immunocytochemistry and RT PCR completed each anothe.

scholarly journals Distribution of high risk Human Papilloma Virus genotypes among cervical cancer patients in a tertiary level hospital in Bangladesh

2019 ◽  
Vol 45 (2) ◽  
pp. 86-92
Author(s):  
Munira Jahan ◽  
Tania Islam ◽  
Sharmin Sultana ◽  
Monira Pervin ◽  
Prof. Ashrafunnessa ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Human Papilloma Virus ◽  
Cancer Patients ◽  
Hpv Infection ◽  
Multiple Infections ◽  
Hpv 16 ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Hpv 18

Background: Cervical cancer is one of the most common types of cancer affecting women worldwide. Persistent HPV infection plays a major role in cervical cancer. The risk of cervical cancer has increased in parallel with the incidence of certain genotypes of human papilloma virus (HPV). Methods: The study was aimed to detect the distribution of HPV genotypes among cervical cancer patients from a specialized hospital in Dhaka, Bangladesh. HPV DNA testing was done by polymerase chain reaction (PCR) using SPF-10 broad-spectrum primers followed by genotyping by reverse hybridization using the INNO-LIPA genotyping system at the Department of Virology, Bangabandhu Sheikh Mujib Medical University, Dhaka. Results: HPV 16 was more prevalent (72.0%) in cervical cancer patients from Bangladesh followed by type 18 (6.0%) and 45 (2.0%). Genotype 16 and 18 alone and as co-infection were detected in 88.0% cases. Multiple HPV infection was found in 20% patients. Along with high risk (HR) HPV 16, 18 and 45 HR HPV 56, 39, 31 and 58 were also prevalent in multiple infections. Conclusion: Thus the study concluded that HR HPV 16 and HPV 18 were more prevalent genotypes among cervical cancer patients in a specialised hospital in Bangladesh. Along with HR HPV 16 and HPV 18, HR HPV 45, 56, 39, 31 and 58 were also prevalent Bangladesh Med Res Counc Bull 2019; 45: 86-92

Ultrastructural changes in sciatic nerve tissue: Effects of passive maternal smoking

1983 ◽  
Vol 41 ◽  
pp. 650-651
Author(s):  
Amankwah K.S. ◽  
A.D. Weberg ◽  
R.C. Kaufmann
Keyword(s):  
Sciatic Nerve ◽  
Maternal Smoking ◽  
Ultrastructural Level ◽  
Uranyl Acetate ◽  
Nerve Tissue ◽  
Ultrastructural Changes ◽  
Cacodylate Buffer ◽  
And Control ◽  
Spontaneous Delivery

Previous research has revealed that passive (involuntary inhalation) tobacco smoking during gestation can have adverse effects upon the developing fetus. These prior investigations did not concentrate on changes in fetal morphology. This study was undertaken to delineate fetal neural abnormalities at the ultrastructural level in mice pups exposed in utero to passive maternal smoking.Pregnant study animals, housed in a special chamber, were subjected to cigarette smoke daily from conception until delivery. Blood tests for determination of carbon monoxide levels were run at 15-18 days gestation. Sciatic nerve tissue from experimental and control animals were obtained following spontaneous delivery and fixed in 2.5% gluteraldehyde in 0.1M cacodylate buffer pH 7.3. The samples were post-fixed in osmium ferrocyanide (1:1 mixture of 1.5% aqueous OSO4 and 2.5% K4 Fe(CN)6). Following dehydration, the tissues were infiltrated with and embedded in Spurr. Sections were stained with uranyl acetate and lead citrate.

Application of EDS Technique for OSP Film Thickness Measurement

2006 ◽  
Author(s):  
Prong Kongsubto ◽  
Sirarat Kongwudthiti
Keyword(s):  
Thin Film ◽  
Quantitative Analysis ◽  
Joint Strength ◽  
Critical Factor ◽  
Thickness Measurement ◽  
Ic Manufacturing ◽  
And Control ◽  
Film Thickness Measurement ◽  
Non Destructive

Abstract Organic solderability preservatives (OSPs) pad is one of the pad finishing technologies where Cu pad is coated with a thin film of an organic material to protect Cu from oxidation during storage and many processes in IC manufacturing. Thickness of OSP film is a critical factor that we have to consider and control in order to achieve desirable joint strength. Until now, no non-destructive technique has been proposed to measure OSP thickness on substrate. This paper reports about the development of EDS technique for estimating OSP thickness, starting with determination of the EDS parameter followed by establishing the correlation between C/Cu ratio and OSP thickness and, finally, evaluating the accuracy of the EDS technique for OSP thickness measurement. EDS quantitative analysis was proved that it can be utilized for OSP thickness estimation.

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