scholarly journals Synergistic effect of cytokinins on in vitro propagation of Stereospermum suaveolens using nodal explants

2021 ◽  
Vol 19 (3) ◽  
2007 ◽  
Vol 1 (1) ◽  
pp. 15-18
Author(s):  
Chandran C ◽  
◽  
Karthikeyan K ◽  
Kulothungan S ◽  
◽  
...  

2007 ◽  
Vol 44 (07) ◽  
pp. 514-519 ◽  
Author(s):  
Ved Prakash Pandey ◽  
Jose Kudakasseril ◽  
Elizabeth Cherian ◽  
George Patani ◽  

Two different methods of in vitro multiplication of Rauwolfia serpentina from nodal explants were compared viz. multiplication via callus morphogenesis and that via shoot proliferation from axillary buds. The second method was found to be far better. The optimum shoot proliferation occurred on Murashige and Skoog (MS) medium supplemented with 1 mg/L naphthalene acetic acid (NAA) and 2 mg/L of benzyl aminopurine (BAP). The best rooting of shoots occurred on MS medium containing 4% sucrose and 1 mg/L of NAA. Solid and liquid MS media were found to be similar in supporting shoot proliferation. The plants produced were successfully hardened and established in soil. An easy, reliable and reproducible protocol was developed for in vitro micropropagation of Rauwolfia serpentina from nodal explants.


2017 ◽  
pp. 87-90
Author(s):  
S.M. Brunda ◽  
C. Lekha Rani ◽  
P. Rajendran ◽  
R. Smitha ◽  
L. Priya

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