Comparison of Oxacillin Disc Diffusion Test with mecA Polymerase Chain Reaction and Cefoxitin Disc Diffusion Test for the Detection of Oxacillinresistant Staphylococcus aureus Collected from Baghdad Hospitals

2014 ◽  
Vol 17 (2) ◽  
pp. 172-180
Author(s):  
Dhafar Al-Ugaili ◽  
◽  
A-M.A. Fadhil ◽  
Saleh A. Wohaieb ◽  
◽  
...  
2001 ◽  
Vol 64 (5) ◽  
pp. 664-668 ◽  
Author(s):  
SUDHIR TAMARAPU ◽  
JOHN L. McKILLIP ◽  
MARYANNE DRAKE

A multiplex polymerase chain reaction (PCR) assay was developed for the detection and differentiation of enterotoxigenic Staphylococcus aureus in dairy products. A solvent extraction procedure was successfully modified for extraction of S. aureus DNA from 10 ml of artificially contaminated skim milk or 20 g cheddar cheese. Primers targeting the enterotoxin C gene (entC) and thermostable nuclease gene (nuc) were used in the multiplex PCR. PCR products were confirmed using restriction fragment length polymorphism analysis. DNA was consistently quantified and amplified by uniplex PCR from 10 CFU/ml of S. aureus in skim milk or 10 CFU/20 g cheddar cheese. The sensitivity of the multiplex PCR was 100 CFU/ml of skim milk or 100 CFU/20 g cheddar cheese. The developed methodology allows presumptive identification and differentiation of enterotoxigenic S. aureus in less than 6 h.


Sign in / Sign up

Export Citation Format

Share Document