scholarly journals Pengaruh Sodium Hipoklorit dan Kalsium Hipoklorit terhadap Daya Hidup Leptospira Patogenik

2021 ◽  
Vol 49 (3) ◽  
pp. 175-182
Author(s):  
Arief Nugroho ◽  
Esti Rahardianingtyas ◽  
Rendro Wianto ◽  
Nurhidayati Nurhidayati ◽  
Farida Dwi Handayani
Keyword(s):  
Experimental Design ◽  
Sodium Hypochlorite ◽  
Laboratory Study ◽  
Active Ingredient ◽  
Health Problem ◽  
Active Ingredients ◽  
Calcium Hypochlorite ◽  
Pathogenic Leptospira ◽  
Significant Difference ◽  
Statistical Results

Leptospirosis is still a health problem in the word. Leptospirosis can be transmitted to humans trough contact with environment infected with pathogenic Leptospira. Efforts to control pathogenic Leptospira in the environment can be done one of them by disinfecting. Effective desinfectants for the control of pathogenic Leptospira include the active ingredients Calcium hypochlorite 60% and Sodium hypochlorite 5,25%. This study aims to determine the effectiveness of Calcium hypochlorite 60% and Sodium hypochlorite 5,25% against the life power of pathogenic Leptospira. This research is a laboratory study with pure experimental design. Serovar of pathogenic Leptospira used is Leptospira icterohaemorrhagiae. Leptospira icterohaemorrhagiae desnsity used as much as 5.7 x 106 Leptospira/ml. The concentrations of desinfectans and bacteria are: 5 ppm, 50 ppm, 500 ppm, 1000 ppm, 2000 ppm, 5000 ppm, 10.000 ppm, and 25.000 ppm. Observations are made every ten minutes and twenty minutes with three repeats. The results showed that Leptospira icterohaemorrhagiae could not survive when tested on 60% calcium hypochlorite and 5.25% sodium hypochlorite, respectively, at a concentration of 2000 ppm and a concentration of 1000 ppm. Statistical results showed there was a significant difference in the concentration of each disinfectant active ingredient. Thus, a disinfectans of chlorine with the active ingredient Calcium hypochlorite 60% and Sodium hypochlorite 5,25% effectively affects the viability of the Leptospira icterohaemorrhagiae. Keywords : Sodium Hypochlorite, Calcium Hypochlorite, Leptospira, Disinfectant Abstrak Leptospirosis masih menjadi masalah kesehatan di dunia. Leptospirosis dapat menular ke manusia lewat kontak dengan lingkungan yang mengandung Leptospira patogenik. Upaya pengendalian Leptospira patogenik di lingkungan dapat dilakukan salah satunya dengan pemberian disinfektan. Disinfektan yang efektif untuk pengendalian Leptospira patogenik diantaranya adalah bahan aktif kalsium hipoklorit 60% dan sodium hipoklorit 5,25%. Penelitian ini bertujuan mengetahui efektivitas bahan aktif kalsium hipoklorit 60% dan sodium hipoklorit 5,25% terhadap daya hidup Leptospira patogenik. Penelitian ini merupakan penelitian skala laboratorium dengan rancangan eksperimental murni. Serovar Leptospira patogenik yang digunakan adalah Leptospira icterohaemorrhagiae. Kepadatan Leptospira icterohaemorrhagiae yang digunakan sebanyak 5,7 x 106 Leptospira/ml. Konsentrasi campuran disinfektan dan bakteri yaitu: 5 ppm, 50 ppm, 500 ppm, 1000 ppm, 2000 ppm, 5000 ppm, 10.000 ppm, dan 25.000 ppm. Pengamatan dilakukan tiap 10 menit dan 20 menit dengan tiga kali ulangan. Hasil penelitian menunjukkan Leptospira icterohaemorrhagiae tidak dapat hidup saat diujikan ke kalsium hipoklorit 60% dan sodium hipoklorit 5,25% berturut-turut pada konsentrasi 2000 ppm dan konsentrasi 1000 ppm. Hasil statistik menunjukkan ada perbedaan signifikan dari besaran konsentrasi masing-masing bahan aktif disinfektan. Dengan demikian, disinfektan klorin dengan bahan aktif calcium hypochlorite 60% dan sodium hypochlorite 5,25% efektif memengaruhi daya hidup Leptospira icterohaemorrhagiae. Kata kunci: Sodium hipoklorit, Kalsium hipoklorit, Leptospira, Disinfektan

Efficacy of Germicidal Hand Wash Agents in Use in a Meat Processing Plant

1987 ◽  
Vol 50 (4) ◽  
pp. 289-295 ◽  
Author(s):  
M. E. STILES ◽  
A. Z. SHEENA
Keyword(s):  
Laboratory Study ◽  
Chlorhexidine Gluconate ◽  
Hand Washing ◽  
Processing Plant ◽  
Meat Processing ◽  
Active Ingredients ◽  
Controlled Conditions ◽  
Hand Wash ◽  
Significant Difference ◽  
Work Units

The in-use efficacy of a selected range of germicidal hand wash agents was tested in a meat processing plant. The hand washes included non-germicidal soaps and germicidal agents containing chlorhexidine, iodophor and Irgasan DP 300 as active ingredients. A laboratory study was done under controlled conditions with standardized procedures for hand washing; in the meat plant, “normal” (unstandardized) hand wash procedures were followed. Levels of contamination on hands varied markedly between work units. Only in the meat cutting area could a significant difference be attributed to hand wash agents against transient-type bacteria on workers' hands. The hand wash agent with 4% chlorhexidine gluconate, the iodophor with 0.75% available iodine and the gel containing 0.3% Irgasan DP 300 were the only products that gave a significantly better reduction of transient bacteria than non-germicidal soap. Transient bacteria were detected on hands after washing, indicating that under the in-use conditions in the meat processing plant, hand wash techniques did not remove all of these bacteria from hands. The plant workers generally indicated a dislike for the iodophor products as hand germicides.

scholarly journals PerkecambahanPeronosclerospora spp. Asal Beberapa Daerah di Jawa Barat pada Fungisida Berbahan Aktif Metalaksil, Dimetomorf dan Fenamidon

Agrikultura ◽  
2018 ◽  
Vol 28 (2) ◽  
Author(s):  
Fitri Widiantini ◽  
Dwisari Januarily Pitaloka ◽  
Ceppy Nasahi ◽  
Endah Yulia
Keyword(s):  
Downy Mildew ◽  
Active Ingredient ◽  
Active Ingredients ◽  
West Java ◽  
Corn Production ◽  
Major Disease ◽  
Conidia Germination ◽  
Significant Difference ◽  
The World ◽  
Corn Plants

ABSTRACTGermination of Peronosclerospora spp. isolated from several maize plantation areas in West Java on fungicides containing active ingredient of metalaxyl, dimetomorph and fenamidoneDowny mildew is a major disease in corn plants that affect the world corn production, include in Indonesia. The control of downy mildew that caused by pathogen Peronosclerospora spp. are relying on the use of synthetic fungicides. However, the emergence of the Peronosclerospora spp. resistant against fungicides rise awareness for the need of constant surveillance. The aim of this study was to know the effectiveness of three commonly used active ingredients fungicide (Metalaxyl, Dimetomorph, and Fenamidone) in controlling Peronosclerospora spp. from five regions in Wes Java (Sumedang District,Bandung District, Majalengka District, Garut District and West Bandung District). Experiment was conducted using conidia germination method. The results of this study demonstrated that fungicide with active ingredient of Dimethomorph was able to suppress the germination of conidia Peronosclerospora spp. with 0% of conidia germination in 4 districts (Sumedang, Bandung, Majalengka and Garut) and only 0.47% conidia germinated in isolate collected from West Bandung District. Meanwhile, fenamidon experienced a decrease in effectiveness at West Bandung District. In this study, it was also found that the number of conidia germinated on isolates treated with Metalaxyl did not show any significant difference compared to control. This suggested that Peronosclerospora spp. might resistant to Metalaxyl.Keywords: Downy mildew, Peronosclerospora spp., metalaxyl, dimetomorph, fenamidoneABSTRAKPenyakit bulai merupakan penyakit utama pada tanaman jagung yang berpengaruh terhadap produksi jagung dunia, termasuk di Indonesia. Pengendalian penyakit bulai yang disebabkan oleh patogen Peronosclerospora spp. masih bertumpu pada penggunaan fungisida sintetik. Akan tetapi, terjadinya penurunan keefektifan fungisida terhadap Peronosclerospora spp. menyebbkan diperlukannya monitoring secara berkala. Penelitian ini bertujuan untuk mengetahui keefektifan tiga jenis bahan aktif fungisida yang umum digunakan (Metalaksil, Dimetomorf, dan Fenamidon) dalam mengendalikan penyakit bulai terhadap patogen Peronosclerospora spp.asal lima daerah di Jawa Barat (Kab. Sumedang, Kab. Bandung, Kab. Majalengka, Kab. Garut dan Kab. Bandung Barat). Pengujian keefektifan fungisida dilakukan dengan menggunakan metode perkecambahan konidia. Hasil penelitian menunjukkan bahwa perlakuan fungsida dimetomorf terhadap isolat Peronosclerospora spp. asal semua daerah dapat menekan perkecambahan konidia dengan persentase perkecambahannya 0% pada 4 daerah dan 0,47% pada isolat asal daerah Kab. Bandung Barat. Sementara Fenamidon menujukkan indikasi penurunan keefektifan di daerah Bandung Barat. Pada penelitian ini, ditemukan pula adanya indikasi resistensi patogen Peronosclerospora spp. terhadap fungisida Metalaksil. Hal ini terlihat dari perkecambahan konidia yang diperlakukan dengan Metalaksil yang hasilnya tidak berbeda nyata dengan kontrol yang tidak diperlakukan dengan fungisida.Kata Kunci: Penyakit bulai jagung, Peronosclerospora spp., Metalaksil, Dimetomorf, Fenamidon

scholarly journals Effectiveness of calcium and sodium hypochlorite associated with passive ultrasonic irrigation on pulp tissue dissolution – An in vitro study

2016 ◽  
Vol 31 (3) ◽  
pp. 109
Author(s):  
Matheus Albino Souza ◽  
Ana Paula Almeida ◽  
Vitória Menin ◽  
Daniel Lima Dalla Lana ◽  
Ana Paula Farina ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Pulp Tissue ◽  
Calcium Hypochlorite ◽  
Passive Ultrasonic Irrigation ◽  
Significant Difference ◽  
Before And After ◽  
Ultrasonic Irrigation ◽  
Group 5

Objectives: To compare, in vitro, the effectiveness of calcium hypochlorite and sodium hypochlorite associated with passive ultrasonic irrigation (PUI) on pulpal tissue dissolution.Methods: The pulp tissue of forty-five bovine incisors was removed and each complete pulp was divided into two pieces of similar volume, resulting in 90 pieces distributed into nine groups (n=10), according to dissolution procedure: G1: distilled water; G2: 2.5% NaOCl; G3: 5.25% NaOCl; G4: 2.5% Ca(OCl)2; G5: 5.25% Ca(OCl)2; G6: 2.5% NaOCl+PUI; G7: 5.25% NaOCl+PUI; G8: 2.5%Ca(OCl)2+PUI and G9: 5.25% Ca(OCl)2+PUI. Each sample was weighed on a high precision balance before and after the dissolution procedure. The differences in weights were calculated and the mean percentage loss was determined. The data were statistically analyzed using two-way ANOVA and Games-Howel tests at 5% significance level.Results: Group 5 (5.25% Ca(OCl)2), 6 (2.5% NaOCl+PUI), 7 (5.25% NaOCl+PUI), 8 (2.5% Ca(OCl)2+PUI) and 9 (5.25% Ca(OCl)2+PUI) showed the highest mean percentages of weight reduction when compared to other groups (p<0.001). However, there was no statistically significant difference between them (p<0.001).Conclusions: Ca(OCl)2 and PUI contributes significantly to dissolution of pulp tissue.

scholarly journals Perbedaan Tekanan Darah Sebelum dan Sesudah Pekerja Kopi Terpajan Kebisingan di Penggilingan Padi

2019 ◽  
Vol 1 (1) ◽  
pp. 27-37
Author(s):  
Muhammad Amin ◽  
Rita Rosari ◽  
Yenida Yenida
Keyword(s):  
Blood Pressure ◽  
Experimental Design ◽  
Diastolic Blood Pressure ◽  
Research Design ◽  
Quantitative Research ◽  
Sampling Technique ◽  
Significant Difference ◽  
Before And After ◽  
Research Type ◽  
Statistical Results

This study aims to determine differences in blood pressure before and after workers exposed to noise in rice and coffee milling in Kepahiang Regency. This research was conducted in July 2017 with quantitative research type and research design which used pre experimental design with one group pretest posttest approach. The sampling technique used is total sampling with sample is 22 workers at point 1 had increased sistole blood pressure (39.29%) and 17 workers at point 2 did not increase Sistole blood pressure (30.36%). While for diastolic blood pressure, there were 14 workers at point 1 had an increase in diastolic blood pressure (25%) and 18 workers point 2 did not increase diastolic blood pressure (30%). Based on Wil Coxon Signed Ranks Test statistical results obtained p = 0.000 <α (0.05) for blood pressure of laborer systole point 1, p = 0.000 <α (0.05) for blood pressure sistole worker point 2, p = 0.000 <α (0.05 ) for diastolic blood pressure point 2, and p = 0.002 <α (0.05) for the worker's diastolic blood pressure at point 2. So there is a significant difference in blood pressure before and after workers exposed to noise in rice and coffee milling in Kepahiang Regency.   Keywords: Blood Pressure, Noisy

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

scholarly journals Listening to Slugs: Acceptability and Consumption of Molluscicide Pellets by the Grey Field Slug, Deroceras reticulatum

Insects ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 548
Author(s):  
Samantha Mirhaya de Silva ◽  
David Chesmore ◽  
Jack Smith ◽  
Gordon Port
Keyword(s):  
United Kingdom ◽  
Economic Impact ◽  
Active Ingredient ◽  
Crop Protection ◽  
Crop Plants ◽  
Deroceras Reticulatum ◽  
The United Kingdom ◽  
Ferric Phosphate ◽  
Significant Difference

Gastropod damage to crop plants has a significant economic impact on agricultural and horticultural industries worldwide, with the Grey Field Slug (Deroceras reticulatum (Müller)) considered the main mollusc pest in the United Kingdom and in many other temperate areas. The prevailing form of crop protection is pellets containing the active ingredient, metaldehyde. Metaldehyde can cause paralysis and death in the mollusc, depending on the amount ingested. The paralysing effects may result in reduced pellet consumption. A greater understanding of metaldehyde consumption may reveal an area that can be manipulated using novel molluscicide formulations. Novel pellet types included commercial metaldehyde pellets coated so that metaldehyde is released more slowly. In both laboratory and arena trials, an audio sensor was used to record individual slugs feeding on a variety of pellet types, including commercially available toxic pellets (metaldehyde and ferric phosphate) and novel metaldehyde formulations. The sensor was used to record the length of each bite and the total number of bites. There was no significant difference in the length of bites between pellet types in laboratory trials. Novel pellets were not consumed more than commercial pellet types. Commercial pellet types did not differ in consumption.

scholarly journals Nutritional Status Disorders in Student Population

2019 ◽  
Vol 0 (0) ◽  
Author(s):  
Djordje Stevanovic ◽  
Mina Poskurica ◽  
Jovan Jovanovic ◽  
Miodrag Sreckovic ◽  
Vladimir Zdravkovic ◽  
...  
Keyword(s):  
Physical Activity ◽  
Nutritional Status ◽  
Health Problem ◽  
Eating Habits ◽  
Student Population ◽  
Regular Physical Activity ◽  
Cross Sectional ◽  
Major Health Problem ◽  
Unhealthy Eating ◽  
Significant Difference

Abstract Obesity is a global health problem associated with numerous pathological conditions. Unhealthy eating habits and the lack of regular physical activity are considered the most common cause of disordered nutritional status. The aim of the research was to determine the nutritional status in student population and the predictors which determine this condition. The cross-sectional study was conducted on 262 students of the Faculty of Medical Sciences in Kragujevac (130 males and 132 females). Body weight and height, body mass index (BMI) and visceral fat (VF) were measured. Each respondent completed a specially designed questionnaire considering sociodemographic data, eating habits and physical activity. The majority of students have normal BMI values (75.6%), 5.3% were classified as underweight, 14.9% as over-weight and 4.3% as obese. Normal VF values were found in 93.1% of subjects, while high in 5.7% and very high in 1.1%. A statistically significant difference in BMI and VF values was found between male and female gender (24.41 vs. 21.05, Sig = 0.000 and 5.47 vs. 3.07, Sig = 0.000, respectively), as well as between students of the first 4 and the last 2 years of study (Sig = 0.019 and 0.000 respectively). Unhealthy eating habits, such as the consumption of sweets, snacks, fast foods and white bread, and the absence of regular physical activity were statistically more present in overweight/obese respondents. Given the significant presence of pre-obesity/obesity in the examined population, corrective measures should be taken in this population in order to avoid a major health problem in the future.

scholarly journals Sleep Deprivation and Sleep-Onset Insomnia are Associated with Blunted Physiological Reactivity to Stressors

2021 ◽  
Vol 186 (Supplement_1) ◽  
pp. 246-252
Author(s):  
Devon A Hansen ◽  
Brieann C Satterfield ◽  
Matthew E Layton ◽  
Hans P A Van Dongen
Keyword(s):  
Sleep Deprivation ◽  
Laboratory Study ◽  
Salivary Cortisol ◽  
Sleep Onset ◽  
Deprivation Condition ◽  
Increased Risk ◽  
Significant Difference ◽  
Sleep Deficiency ◽  
The Impact ◽  
Chronic Sleep

ABSTRACT Introduction Military operations often involve intense exposure to stressors combined with acute sleep deprivation, while military personnel also experience high prevalence of chronic sleep deficiency from insomnia and other sleep disorders. However, the impact of acute and chronic sleep deficiency on physiologic stressor responses is poorly understood. In a controlled laboratory study with normal sleepers and individuals with chronic sleep-onset insomnia, we measured responses to an acute stressor administered in a sleep deprivation condition or a control condition. Methods Twenty-two adults (aged 22-40 years; 16 females)—11 healthy normal sleepers and 11 individuals with sleep-onset insomnia—completed a 5-day (4-night) in-laboratory study. After an adaptation day and a baseline day, subjects were assigned to a 38-hour total sleep deprivation (TSD) condition or a control condition; the study ended with a recovery day. At 8:00 PM after 36 hours awake in the sleep deprivation condition or 12 hours awake in the control condition, subjects underwent a Maastricht Acute Stress Test (MAST). Salivary cortisol was measured immediately before the MAST at 8:00 PM, every 15 minutes after the MAST from 8:15 PM until 9:15 PM, and 30 minutes later at 9:45 PM. Baseline salivary cortisol was collected in the evening of the baseline day. Additionally, before and immediately upon completion of the MAST, self-report ratings of affect and pain were collected. Results The MAST elicited a stressor response in both normal sleepers and individuals with sleep-onset insomnia, regardless of the condition, as evidenced by increases in negative affect and pain ratings. Relative to baseline, cortisol levels increased immediately following the MAST, peaked 30 minutes later, and then gradually returned to pre-MAST levels. At the cortisol peak, there was a significant difference across groups and conditions, reflecting a pronounced blunting of the cortisol response in the normal sleepers in the TSD condition and the sleep-onset insomnia group in both the TSD and control conditions. Conclusions Blunted stressor reactivity as a result of sleep deficiency, whether acute or chronic, may reflect reduced resiliency attributable to allostatic load and may put warfighters at increased risk in high-stakes, rapid response scenarios.

The Effect of Bleach Liquid and Household Cleaner on Surface Hardness and Roughness of Acrylic Base of Orthodontic Appliances

2021 ◽  
Author(s):  
Saeed Noorollahian ◽  
Farinaz Shirban ◽  
Vahid Mojiri
Keyword(s):  
Surface Roughness ◽  
Hydrochloric Acid ◽  
Sodium Hypochlorite ◽  
Surface Hardness ◽  
Acrylic Resin ◽  
Liquid Sodium ◽  
P Value ◽  
Orthodontic Appliances ◽  
Acrylic Resins ◽  
Significant Difference

Introduction: The daily use of orthodontic removable plates can interfere with the self-cleansing function of the mouth. Although various techniques have been proposed for cleaning removable orthodontic appliances, there is no consensus on the use of a safe method that preserves the physical properties of the appliance. This study aimed to investigate the effect of using hydrochloric acid (10%, for removal of mineral deposits) and sodium hypochlorite (5.25%, to remove organic matter and discolorations) on surface hardness and roughness of self-cure orthodontic acrylic resins. Materials & Methods: This cross-sectional laboratory study was conducted in the fall of 2017 at Isfahan University of Medical science and Isfahan University of Technology. In this study two orthodontic acrylic resins (Orthocryl® and Acropars®) were used. Eighty samples (12×10×3 mm) from each one were fabricated and divided into four groups (n = 20). Group 1: 15 minutes immersion in household cleaner liquid (Hydrochloric acid, 10%) followed by15 minutes immersion in household bleach liquid (Sodium Hypochlorite, 5.25%). In group 2, immersions were repeated just like group two times and in group 3, were done three times. Group 4 was as control and had no immersion. The surface hardness and roughness of samples were measured. Data were analyzed with Two Way ANOVA and the significance level was set at 0.05. Results: The number of immersion procedures did not significantly affect the surface hardness (p value = 0.958) and surface roughness (p value = 0.657) in the different study groups. There was no significant difference in the surface hardness between the two acrylic resin brands (p value = 0.077); however, Acropars acrylic resin samples exhibited significantly higher surface roughness compared to the Orthocryl acrylic resin samples(p value < 0.001). Conclusion: 15 minutes of immersion in 10% HCl, followed by 15 minutes of immersion in 5.25% NaOCl and repetition of the procedure three times did not significantly affect the surface hardness and roughness of self-cured acrylic resins.

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