PENGARUH KOMBINASI IAA (Indole-3-Acetic Acid) DAN BAP (6-Benzylaminopurine) TERHADAP INISIASI TANAMAN VANILI (Vanilla planifolia Andrews)

Provision of Vanilla (Vanilla planifolia Andrews) with traditional cultivation often has problems, including the availability of unhealthy seeds due to disease caused by Fusarium oxysporum. This disease can thwart vanilla plantations up to 85% because the pathogen can infect all parts of the vanilla plant making it difficult to control efforts. Alternative efforts were made to overcome this problem, namely through the initiation process in tissue culture by combining the hormones IAA (indole-3-acetic acid) and BAP (6-benzylaminopurine). This study aims to determine the effect and the maximum concentration of the combination of IAA and BAP on the initiation of vanilla plants. This study was conducted based on a completely randomized design (CRD) with 5 treatments and 3 repetitions. This treatment consisted of: A (MS + 0.1 ppm IAA + 1 ppm BAP), B (MS + 0.2 ppm IAA + 1 ppm BAP), C (MS + 0.3 ppm IAA + 1 ppm BAP), D (MS + 0.4 ppm IAA + 1 ppm BAP), and E (MS + 0.5 ppm IAA + 1 ppm BAP). Observations were made after 40 days from the planting process. The results showed that the concentration of the combination of IAA and BAP can affect the growth and organogenesis of the initiation of vanilla plants (Vanilla planifolia Andrews). Treatment C (MS + 0.3 ppm IAA + 1 ppm BAP) was the best concentration from this study, with average values: number of shoots (1), shoot length (1.73 cm), number of roots (1), and number of leaves (1.33).

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Induksi Kalus Kentang Asal Desa Dombu (Solanum tuberosum L.) Dengan Zpt Indole-3-Acetic Acid (IAA)

Natural Science Journal of Science and Technology ◽

10.22487/25411969.2019.v8.i2.13546 ◽

2019 ◽

Vol 8 (2) ◽

Author(s):

Lyly Zulraufianti ◽

Asri Pirade Paserang

Keyword(s):

Tissue Culture ◽

Acetic Acid ◽

Solanum Tuberosum ◽

Natural Science ◽

Callus Induction ◽

Solanum Tuberosum L ◽

Randomized Design ◽

Completely Randomized Design ◽

Department Faculty ◽

Indole 3 Acetic Acid

The research of callus induction in potato (S. tuberosum L.) from Dombu Village with indole-3-acetic-acid (IAA) was conducted from Januari to April 2019 in Laboratory of Tissue Culture, Biology Department, Faculty of Mathematics and Natural Science, Tadulako University. The research was aimed to determine of concentration IAA of the best to induce callus. This study was designed based on Completely Randomized Design (CRD) with 5 treatments, 3 replications and 3 explants on each unit. The treatments were P1= MS + 0 ppm, P2= MS + 0.5 ppm, P3= MS + 1.0 ppm, P4= MS + 2.0 ppm, P5= MS + 3.0 ppm. The results showed that the best and the efficient treatments for induction of callus treatment was P4= MS + 2.0 ppm with to induce callus up to 100%, the callus colour was yellow to brown and intermediate texture.

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Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.6.1.20-28 ◽

2015 ◽

Vol 6 (1) ◽

pp. 20

Author(s):

Erna Sinaga ◽

Megayani Sri Rahayu ◽

Awang Maharijaya

Keyword(s):

Tissue Culture ◽

Drought Tolerance ◽

In Vitro Selection ◽

Solanum Melongena ◽

Survival Percentage ◽

Drought Tolerant ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design

ABSTRACTThe objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought tolerance of eggplant and the drought tolerant eggplant accessions. The experiment was conducted at the Laboratory of Tissue Culture, Department of Agronomy and Horticulture, Bogor Agricultural University. The experiment was arranged in a completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10, and 15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090). The results showed that the addition of PEG to in vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought ABSTRAKPenelitian ini bertujuan untuk mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG) terhadap pertumbuhan tanaman terung in vitro, mendapatkan konsentrasi PEG yang dapat digunakan untuk seleksi tanaman terung secara in vitro dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan, Departemen Agronomi dan Hortikultura, Institut Pertanian Bogor. Penelitian ini disusun dalam rancangan acak lengkap dua faktor. Faktor pertama adalah konsentrasi PEG terdiri atas 0, 5, 10, dan 15%. Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090). Hasil penelitian menunjukkan bahwa penambahan PEG pada media in vitro memberikan pengaruh nyata dan sangat nyata terhadap persentase hidup eksplan, persentase eksplan berkalus, pertambahan tinggi tunas, dan jumlah daun tanaman terung. Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan

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VEGETATIVE PROPAGATION OF Spondias tuberosa E Spondias dulcis WITH THE USE OF IMMERSION IN INDOLE ACETIC ACID

Revista Caatinga ◽

10.1590/1983-21252019v32n401rc ◽

2019 ◽

Vol 32 (4) ◽

pp. 858-866

Author(s):

GABRIELA TEODORO ROCHA ◽

AYURE GOMES DA SILVA ◽

JULIANA BEZERRA MARTINS ◽

NEI PEIXOTO ◽

FABRICIO RODRIGUES

Keyword(s):

Acetic Acid ◽

Vegetative Propagation ◽

Indole Acetic Acid ◽

Stem Cuttings ◽

Randomized Design ◽

Spondias Tuberosa ◽

Completely Randomized Design ◽

Low Efficiency ◽

Indole 3 Acetic Acid ◽

Total Fresh Weight

ABSTRACT The objective of this work was to evaluate the effect of six indole-3-acetic acid (IAA) concentrations and three immersion times in IAA solutions on the vegetative propagation of Spondias dulcis and Spondias tuberosa by stem cuttings. The experiment was conducted in a greenhouse, using a completely randomized design, with a 6×3 factorial arrangement consisting of six indole-3-acetic acid concentrations (0, 2, 4, 6, 8, and 10 g L-1) and three immersion times (8, 16, and 24 seconds), with ten cuttings of S. dulcis and S. tuberosa per plot, and three replications. Percentage of surviving cuttings, and percentage of rooted cuttings, number of leaf buds, number of roots per cutting, root length, and total fresh weight were evaluated at 180 days after planting. Data were subjected to analysis of variance by the F test and to regression analysis. Vegetative propagation from woody cuttings of S. dulcis is not viable to produce seedlings, thus, new studies on this technique for this species are needed. Vegetative propagation from woody cuttings of S. tuberosa is satisfactory, but with low efficiency for improvement and emergence of new shoots and roots; it is more effective when using a concentration of 10 g L-1 of indole-3-acetic acid and immersion time in the solution of 16 seconds.

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Multiplikasi in vitro stroberi kultivar Tochiotome dengan penambahan jenis dan konsentrasi sitokinin untuk perbanyakan bibit

Kultivasi ◽

10.24198/kultivasi.v19i3.26932 ◽

2020 ◽

Vol 19 (3) ◽

Author(s):

Ega Raisya ◽

Denny Sobardini Sobarna ◽

Anne Nuraini ◽

Syariful Mubarok ◽

Erni Suminar ◽

...

Keyword(s):

Tissue Culture ◽

Fast Time ◽

Endogenous Auxin ◽

Seed Technology ◽

Randomized Design ◽

Process Plants ◽

Number Of Leaves ◽

Completely Randomized Design

Sari Perbanyakan tanaman stroberi secara konvensional dilakukan dengan menggunakan stolon, tetapi kurang efektif serta kualitas bibit yang dihasilkan kurang baik akibat adanya akumulasi penyakit. Budidaya stroberi memerlukan adanya perbanyakan bibit secara massal, tetapi tidak mengubah kualitasnya. Multiplikasi in vitro menjadi solusi untuk penyediaan bibit berkualitas dalam jumlah besar. Upaya untuk mendapatkan tunas in vitro dalam jumlah banyak yakni perlu adanya penambahan zat pengatur tumbuh golongan sitokinin seperti Benzylaminopurine (BAP) atau Thidiazuron (TDZ). Tujuan penelitian ini adalah mengetahui dan menetapkan jenis serta konsentrasi sitokinin dengan hasil terbaik dalam multiplikasi stroberi kultivar Tochiotome. Percobaan dilaksanakan di Laboratorium Kultur Jaringan Tanaman, Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran. Penelitian menggunakan Rancangan Acak Lengkap yang terdiri dari tujuh perlakuan yang diulang lima kali, yaitu: Kontrol (tanpa sitokinin); BAP (0,25 ppm; 0,50 ppm; 0,75 ppm), dan TDZ (0,25 ppm; 0,50 ppm; 0,75 ppm). Hasil dari percobaan menunjukkan bahwa penambahan sitokinin tidak berpengaruh nyata terhadap jumlah tunas dan bobot segar planlet. Media perlakuan kontrol dapat menghasilkan jumlah akar lebih banyak dibandingkan dengan media ditambah sitokinin. Penambahan BAP 0,50 ppm berpengaruh positif terhadap jumlah daun dan dapat menghasilkan runner secara in vitro. Pemberian BAP 0,50 ppm cenderung dapat meningkatkan dan mempercepat produksi bibit tanaman stroberi kultivar Tochiotome.Kata Kunci: Benzylaminopurine (BAP), Thidiazuron (TDZ), Stroberi, Kultur Jaringan AbstractStolon is used for conventional propagation of strawberry, but it is less effective and the quality of the seeds is not good due to the accumulation of disease. In vitro multiplication becomes a solution for the supply of quality seeds in a fast time. The addition of growth regulator cytokinin, such as Benzylaminopurine (BAP) or Thidiazuron (TDZ) can produced the large number of shoot. The objective of this study was to obtain the best type and concentration of cytokinin in the multiplication of strawberry ‘Tochiotome’. The study was conducted at the Plant Tissue Culture Laboratory, Seed Technology, Faculty of Agriculture, Universitas Padjadjaran. This study used a Completely Randomized Design (CRD) with seven treatments and five replications, that were: Control (without cytokinin); BAP (0.25 ppm; 0.50 ppm; 0.75 ppm), and TDZ (0.25 ppm; 0.50 ppm; 0.75 ppm). The results indicated that addition of cytokinin did not affected increasing number of shoots and fresh weightof plantlets. Control media can produce larger number of roots than those containing PGRs, this might be due to the endogenous auxin concentrations found in strawberry plants. Also, cytokinin inhibited root formations process. Plants treated with BAP 0.50 ppm increased for the number of leaves and produced runners in vitro. This study showed application of BAP with 0.50 ppm increased and accelerated the production of strawberry ‘Tochiotome’ seedlings.Keywords: Benzylaminopurine (BAP), Thidiazuron (TDZ), Strawberry, Tissue Culture

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Respon Setek Cabang Bambu Kuning (Bambusa Vulgaris) Terhadap Pemberian Aia

Jurnal Sylva Lestari ◽

10.23960/jsl1295-100 ◽

2014 ◽

Vol 2 (1) ◽

pp. 95

Author(s):

Yosepin K. Simangunsong ◽

Indriyanto . ◽

Afif Bintoro

Keyword(s):

Acetic Acid ◽

Indole Acetic Acid ◽

Shoot Length ◽

Randomized Design ◽

Honestly Significant Difference ◽

Significant Difference ◽

Bambusa Vulgaris ◽

Bartlett Test ◽

Number Of Leaves ◽

Branch Cutting

Yellow bamboo is one of the bamboo species which used for industrial and house-hold materials, because it has a thick trunk outer layer, length of fiber, and beautiful shape.The success of cuttings grown yellow bamboo branch was lack.To increase the success of the yellow bamboo branches grow cuttings used growth regulators.Therefore, the research was conducted by giving indole acetic acid on yellow bamboo branch cuttings.The objective of this research was to known the growth of yellow bamboo branch cuttings by giving indole acetic acid and known the concentration of the indole acetic acid were the best influence on the growth of yellow bamboo branch cuttings.The research was designed with complete randomized design (CRD) with five treatment,five experimental units, and five branch cuttings for each experimental unit.The concentration ofindole acetic acid (IAA) was 0 ppm, 100 ppm, 200 ppm,300 ppm, and 400 ppm.To test the homogeneity range done with Bartlett test, the data will be tested by analysis of variance and then further tested with honestly significant difference test.Yellow bamboo branch cuttings growth by percentage of life parameters, number of shoots, shoot length, shoot diameter, and a significant number of leaves is known, whereas for root length parameter is not significant. Keywords: branch cutting, indole acetic acid, yellow bamboo

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Pengaruh Konsentrasi Jus Lidah Buaya (Aloe cbinensis Baker) Terhadap Pertumbuhan Planlet Anggrek Hasil Silangan Dendrobium Morning Sun X Dendrobium Samarai

OrchidAgro ◽

10.35138/orchidagro.v1i1.230 ◽

2021 ◽

Vol 1 (1) ◽

pp. 1

Author(s):

Reo Sempana ◽

Lia Amalia ◽

Wahyono Widodo ◽

Elly Roosma Ria ◽

Noertjahyani Noertjahyani

Keyword(s):

Tissue Culture ◽

Experimental Design ◽

Root Length ◽

Aloe Vera ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design ◽

C 50 ◽

Better Than ◽

Number Of Shoots

This experiment aims was to study the effect of the concentration of aloe vera juice and to get the concentration of aloe vera juice which shows better growth of the number of leaves and the number of roots on the Dendrobium orchid plantlet in Hybrid-vitro results. This experiment was carried out at the Tissue Culture Laboratory, Faculty of Agriculture, Winaya Mukti University, which began in May to August 2019. The experimental design was used a Completely Randomized Design with 5 treatments in 5 replications and the treatments namely: A (0 g L-1 solution), B (25 g L-1 solution), C (50 g L-1 solution), D (75 g L-1 solution), E (100 g L-1 solution). The results showed that consentration of B (25 g L-1 solution), C (50 g L-1 solution), D (75 g L-1 solution) gave plantlet height, number of shoots, number of roots, and root length better than another treatments.

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Aklimatisasi dan Respon Pertumbuhan Mutan Leucaena Leucocephala Varietas Tarramba Teradaptasi Asam

Jurnal Ilmu Nutrisi dan Teknologi Pakan ◽

10.29244/jintp.19.3.66-70 ◽

2021 ◽

Vol 19 (3) ◽

pp. 66-70

Author(s):

Muhklisani ◽

Panca Dewi Manuhara Karti ◽

Iwan Prihantoro

Keyword(s):

Tissue Culture ◽

Leucaena Leucocephala ◽

Culture Techniques ◽

Best Response ◽

Randomized Design ◽

Culture Production ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Mutant Lines ◽

Tissue Culture Techniques

Acclimatization is the final stage of plant propagation in tissue culture techniques that can determine the success of the nursery process. This study aimed to observe the growth response during the acclimatization stage of the acid-adapted of Leucaena leucocephala Tarramba variety, which developed from tissue culture techniques. The design used in this study was a completely randomized design (CRD) with the cultivation of 11 mutant lines from tissue culture, namely M1-M11 (an acid-adapted mutant from tissue culture addition of 1 ppm IBA) and 2 controls (lamtoro broodstock without gamma irradiation) namely K0 (lamtoro broodstock resulting from tissue culture addition of 0 ppm IBA), K1 (breeding lamtoro from tissue culture addition of 1 ppm IBA). The variables observed were the level of plant viability, plant height, and number of leaves. The results showed that the acclimatization of the plant Leucaena leucocephala to tissue culture production on the M3 and M9 mutant lines gave the best response to plant morphological growth up to 5 WAP (weeks after planting). Key words: acclimatization, IBA hormone, Leucaena leucocephala, tissue culture

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Clonal propagation of Eucalyptus urophylla under effect of Cyperus rotundus extract and indole-3-acetic acid

Scientia Plena ◽

10.14808/sci.plena.2021.100201 ◽

2021 ◽

Vol 17 (10) ◽

Author(s):

Matheus Gabriel Modesto Santos ◽

Wesley Costa Silva ◽

Pedro Henrique Pinto Ribeiro ◽

Vitor Corrêa de Mattos Barretto ◽

Ednaldo Cândido Rocha ◽

...

Keyword(s):

Acetic Acid ◽

Root Length ◽

Cyperus Rotundus ◽

Root Induction ◽

Eucalyptus Urophylla ◽

Randomized Design ◽

Completely Randomized Design ◽

Tuber Extract ◽

Indole 3 Acetic Acid ◽

Shed Light

Cyperus rotondus tubers possess substances with allelopathic qualities. Some of these substances can act as synergists of plant hormones and be used for root induction in cuttings. This study aimed to shed light on the development and rooting of Eucalyptus urophylla cuttings treated with C. rotondus (nutgrass) tuber extract and IAA (indole-3-acetic acid). The experiments were conducted using a completely randomized design (0; 25; 50; 75 and 100% nutgrass extract and 0; 50; 250; 500; 750 and 1000 mg L-1 of IAA), with four repetitions per treatment and four cuttings per plot. Plantlet height, stem width and root length were measured. The greatest width and height values were recorded for the control (0% nutgrass extract and 0 mg L-1 of IAA), indicating that these substances did not contribute to the development of these variables. However, treatments of nutgrass extract up to 50% were associated with greater root length. C. rotundus extract concentrations of 53% can be used to improve root development in E. urophylla cuttings, and contribute to the production of higher quality plantlets.

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Use of indole-3-acetic acid (IAA) in postharvest of cajá-manga (Spondias dulcis)

Revista Colombiana de Ciencias Hortícolas ◽

10.17584/rcch.2020v14i2.11228 ◽

2020 ◽

Vol 14 (2) ◽

Author(s):

Franciele Cristian Da Silva ◽

Hygor Amaral Santana ◽

Juliana Oliveira Silva De Menezes ◽

Mírian Carvalho Tavares ◽

Rodolfo David Martins ◽

...

Keyword(s):

Acetic Acid ◽

Ascorbic Acid Content ◽

Titratable Acidity ◽

Soluble Solids ◽

Climacteric Fruit ◽

Randomized Design ◽

Postharvest Life ◽

Completely Randomized Design ◽

Indole 3 Acetic Acid ◽

Unripe Fruits

The cajá-manga (Spondias dulcis Parkinson) is an exotic fruit originally from the Polynesian Islands. It is a climacteric fruit, and the postharvest life of its fruits is very short, due to its fast ripening. Thus, this study aimed to evaluate the effect of auxin concentrations on delaying the ripening process of cajá-manga fruits. One hundred twenty cajá-manga fruits obtained from, Hidrolândia-GO, Brazil, at 16°55'32.35" S and 49°21'39.76" W). A completely randomized design in a 5×2+2 factorial scheme was used. Five indole-3-acetic acid (IAA) concentrations (0, 50, 100, 150 and 200 mg L-1), two moments of evaluations (at 5 and 10 days after IAA applications), and two additional treatments (ripe and unripe fruits) were evaluated. Evaluations were carried out every 5 days, in triplicates. The physicochemical variables, pulp firmness, titratable acidity (TA), soluble solids (SS), ascorbic acid content, and color of pulp and peel were evaluated. The concentrations of 50 and 100 mg L-1 of IAA presented the best results, causing a delay in ripening and the lowest sensory losses in the fruits of cajá-manga.

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The Growth of Coastal Cottonwood (Hibiscus tiliaceus Linn.) Seedlings by Inoculating Arbuscular Mychorrhiza Fungi (AMF) on Sand Beach Planting Media

JURNAL BIOLOGI UNAND ◽

10.25077/jbioua.8.2.36-40.2020 ◽

2020 ◽

Vol 8 (2) ◽

pp. 36

Author(s):

Nailul Rahmi Aulya ◽

Zozy Aneloi Noli ◽

Suwirmen Suwirmen

Keyword(s):

Seedling Growth ◽

Dry Weight ◽

Shoot Length ◽

Special Treatment ◽

Sand Beach ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design ◽

High Level ◽

Coastal Land

Hibiscus tiliaceus L. is a seaside plant that has several benefits, one of which is as a producer of paper fiber. H. tiliaceus need a better nutrient to grow on coastal land that is still constrained by the high level of salinity. By this, a special treatment is needed to support its growth. One of them is the application of mycorrhizal inoculants. Mycorrhizae is known as one of the fungi that can help plants tolerate salinity. The aims of the research were to determine the effect of AMF inoculants and determine the best doses of AMF to the seedling growth. The research used completely randomized design with 5 treatments and 5 replications. The treatments were (A) without AMF (control), (B) inoculation AMF 10 g/plant, (C) inoculation AMF 15 g/plant, (D) inoculation AMF 20 g/plant and (E) inoculation AMF 25 g/plant. The result showed that AMF can infect the roots of hibiscus seedlings with an infection degree of 18-34%. However, it has not been able to have a significant effect on shoot length, number of leaves, dry weight of root, and dry weight of shoot.

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