Natural Occurrence and Characterization of the Lyme Disease Spirochete, Borrelia burgdorferi, in Cotton Rats (Sigmodon hispidus) from Georgia and Florida

1995 ◽  
Vol 81 (1) ◽  
pp. 30 ◽  
Author(s):  
James H. Oliver ◽  
Francis W. Chandler ◽  
Angela M. James ◽  
F. Hunt Sanders ◽  
H. Joel Hutcheson ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Natural Occurrence ◽  
Sigmodon Hispidus ◽  
Cotton Rats

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

scholarly journals Characterization of outer membranes isolated from Borrelia burgdorferi, the Lyme disease spirochete.

1995 ◽  
Vol 63 (6) ◽  
pp. 2154-2163 ◽  
Author(s):  
J D Radolf ◽  
M S Goldberg ◽  
K Bourell ◽  
S I Baker ◽  
J D Jones ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Outer Membranes

scholarly journals Characterization of 5′-methylthioadenosine/S-adenosylhomocysteine nucleosidases from Borrelia burgdorferi: Antibiotic targets for Lyme disease

2020 ◽  
Vol 1864 (1) ◽  
pp. 129455 ◽  
Author(s):  
Kenneth A. Cornell ◽  
Reece J. Knippel ◽  
Gerald R. Cortright ◽  
Meghan Fonken ◽  
Christian Guerrero ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi

Cocirculation of Multiple Hantaviruses in Texas, with Characterization of the Small (S) Genome of a Previously Undescribed Virus of Cotton Rats (Sigmodon hispidus)

1996 ◽  
Vol 55 (6) ◽  
pp. 672-679 ◽  
Author(s):  
Julie A. Rawlings ◽  
Susan U. Neill ◽  
Sergio Pichuantes ◽  
Brian Hjelle ◽  
Anton Nguyen ◽  
...  
Keyword(s):  
Sigmodon Hispidus ◽  
Cotton Rats

Characterization of Surface Antigen from Lyme Disease Spirochete Borrelia burgdorferi

2001 ◽  
Vol 289 (2) ◽  
pp. 389-394 ◽  
Author(s):  
Kathryn Jones ◽  
Jesse Guidry ◽  
Pernilla Wittung-Stafshede
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Surface Antigen
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