Isolation of the Protein Backbone of an Arabinogalactan-Protein from the Styles of Nicotiana alata and Characterization of a Corresponding cDNA

1994 ◽  
Vol 6 (11) ◽  
pp. 1643
Author(s):  
He Du ◽  
Richard J. Simpson ◽  
Robert L. Moritz ◽  
Adrienne E. Clarke ◽  
Antony Bacic
Keyword(s):  
Arabinogalactan Protein ◽  
Nicotiana Alata ◽  
Protein Backbone

scholarly journals Characterization of the hydroxyproline-rich protein core of an arabinogalactan-protein secreted from suspension-cultured Lolium multiflorum (Italian ryegrass) endosperm cells

1989 ◽  
Vol 264 (3) ◽  
pp. 857-862 ◽  
Author(s):  
P A Gleeson ◽  
M McNamara ◽  
R E H Wettenhall ◽  
B A Stone ◽  
G B Fincher
Keyword(s):  
Polyclonal Antibodies ◽  
Lolium Multiflorum ◽  
Italian Ryegrass ◽  
Arabinogalactan Protein ◽  
Myeloma Protein ◽  
Protein Core ◽  
Liquid Suspension ◽  
Peptide Component ◽  
Immunodominant Epitopes

An arabinogalactan-protein (AGP) purified from the filtrate of liquid-suspension-cultured Italian-ryegrass (Lolium multiflorum) endosperm cells by affinity chromatography on myeloma protein J539-Sepharose was deglycosylated with trifluoromethanesulphonic acid to remove polysaccharide chains that are covalently associated with hydroxyproline residues in the peptide component of the proteoglycan. The protein core, which accounts for less than 10% (w/w) of the intact proteoglycan, was purified by h.p.l.c. It has an apparent Mr of 35,000, but reacts very poorly with both Coomassie Brilliant Blue R and silver stains. Amino-acid-sequence analysis of the N-terminus of the h.p.l.c.-purified protein core and of tryptic peptides generated from the unpurified protein reveals a high content of hydroxyproline and alanine. These are sometimes arranged in short (Ala-Hyp) repeat sequences of up to six residues. Polyclonal antibodies raised against the protein core do not cross-react with native AGP, the synthetic peptide (Ala-Hyp)4, poly-L-hydroxyproline or poly-L-proline. The results suggest that the polysaccharide chains in the native AGP render the protein core of the proteoglycan inaccessible to the antibodies and that the immunodominant epitopes include domains of the protein other than those rich in Ala-Hyp repeating units.

scholarly journals Characterization of the Arabinogalactan Protein 31 (AGP31) ofArabidopsis thaliana

2012 ◽  
Vol 287 (12) ◽  
pp. 9623-9632 ◽  
Author(s):  
May Hijazi ◽  
Jessica Durand ◽  
Carole Pichereaux ◽  
Frédéric Pont ◽  
Elisabeth Jamet ◽  
...  
Keyword(s):  
Arabinogalactan Protein ◽  
Ofarabidopsis Thaliana

scholarly journals Isolation and Characterization of Arabinogalactan-protein from the Fruit of Lycium chinense Mill.

2000 ◽  
Vol 47 (2) ◽  
pp. 155-161 ◽  
Author(s):  
Xiaoming Qin ◽  
Ryo Yamauchi ◽  
Koichi Aizawa ◽  
Takahiro Inakuma ◽  
Koji Kato
Keyword(s):  
Arabinogalactan Protein ◽  
Lycium Chinense ◽  
Isolation And Characterization

scholarly journals Identification and Characterization of a Prevacuolar Compartment in Stigmas of Nicotiana alata

1999 ◽  
Vol 11 (8) ◽  
pp. 1499-1508 ◽  
Author(s):  
Elizabeth A. Miller ◽  
Marcus C. S. Lee ◽  
Marilyn A. Anderson
Keyword(s):  
Nicotiana Alata ◽  
Prevacuolar Compartment ◽  
Identification And Characterization

scholarly journals Characterization of Synthetic Hydroxyproline-Rich Proteoglycans with Arabinogalactan Protein and Extensin Motifs in Arabidopsis

2006 ◽  
Vol 142 (2) ◽  
pp. 458-470 ◽  
Author(s):  
José M. Estévez ◽  
Marcia J. Kieliszewski ◽  
Natalie Khitrov ◽  
Chris Somerville
Keyword(s):  
Arabinogalactan Protein

scholarly journals Characterization of the Arabidopsis Lysine-Rich Arabinogalactan-Protein AtAGP17 Mutant (rat1) That Results in a Decreased Efficiency of Agrobacterium Transformation

2004 ◽  
Vol 135 (4) ◽  
pp. 2162-2171 ◽  
Author(s):  
Yolanda Maria Gaspar ◽  
Jaesung Nam ◽  
Carolyn Jane Schultz ◽  
Lan-Ying Lee ◽  
Paul R. Gilson ◽  
...  
Keyword(s):  
Agrobacterium Transformation ◽  
Arabinogalactan Protein

Structural Characterization of an Arabinogalactan-Protein from the Fruits of Lycium ruthenicum

2012 ◽  
Vol 60 (37) ◽  
pp. 9424-9429 ◽  
Author(s):  
Qiang Peng ◽  
Jingjing Song ◽  
Xiaopeng Lv ◽  
Zhongfu Wang ◽  
Linjuan Huang ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Arabinogalactan Protein ◽  
Lycium Ruthenicum
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