scholarly journals In vitro digestion of bacterial and plant fructans and effects on ammonia accumulation in cow and sheep rumen fluids.

1998 ◽  
Vol 44 (2) ◽  
pp. 167-171 ◽  
Author(s):  
David R. Biggs ◽  
Kerrie R. Hancock
1959 ◽  
Vol 10 (1) ◽  
pp. 71 ◽  
Author(s):  
PJ Ries ◽  
RL Reid

The effect of pH and of glucose on ammonia accumulation in rumen liquor, obtained from sheep rumen contents, was studied during 4-hr in vitro incubations. Control of pH over the range 4.5-7.3 was effected with sodium phosphate-citric acid buffer. Casein hydrolysate was added as a substrate for ammonia production. There was an optimum pH for ammonia accumulation which varied between 6.0 and 6 . 7 on different diets, and which appeared to be correlated with the range of pH normally encountered in the rumen on each diet. Ammonia accumulation fell rapidly on the acid side of the optimum pH and usually less rapidly on the alkaline side. This effect of pH is most probably due to an effect on ammonia production, i.e. an effect on deamination of amino acids. Glucose reduced ammonia accumulation to an extent greater than could be accounted for by the fall in pH due to the fermentation of glucose; protein nitrogen levels in the reaction mixture were significantly increased. It was concluded that the effect of glucose was due largely to a stimulation of ammonia utilization for synthesis of microbial protein and not to an inhibition of ammonia production.


1997 ◽  
Vol 37 (Suppl. 1) ◽  
pp. 57-58 ◽  
Author(s):  
D. Macheboeuf ◽  
M. Jestin ◽  
M. Chenost

2020 ◽  
Vol 328 ◽  
pp. 127126 ◽  
Author(s):  
Stefano Nebbia ◽  
Marzia Giribaldi ◽  
Laura Cavallarin ◽  
Enrico Bertino ◽  
Alessandra Coscia ◽  
...  

2019 ◽  
Vol 141 ◽  
pp. 240-246 ◽  
Author(s):  
Hui Zhang ◽  
Zhi Li ◽  
Yanjun Tian ◽  
Zibo Song ◽  
Lianzhong Ai

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 410 ◽  
Author(s):  
Kristine Bach Korsholm Knudsen ◽  
Christine Heerup ◽  
Tine Røngaard Stange Jensen ◽  
Xiaolu Geng ◽  
Nikolaj Drachmann ◽  
...  

Efficient lipid digestion in formula-fed infants is required to ensure the availability of fatty acids for normal organ development. Previous studies suggest that the efficiency of lipid digestion may depend on whether lipids are emulsified with soy lecithin or fractions derived from bovine milk. This study, therefore, aimed to determine whether emulsification with bovine milk-derived emulsifiers or soy lecithin (SL) influenced lipid digestion in vitro and in vivo. Lipid digestibility was determined in vitro in oil-in-water emulsions using four different milk-derived emulsifiers or SL, and the ultrastructural appearance of the emulsions was assessed using electron microscopy. Subsequently, selected emulsions were added to a base diet and fed to preterm neonatal piglets. Initially, preterm pigs equipped with an ileostomy were fed experimental formulas for seven days and stoma output was collected quantitatively. Next, lipid absorption kinetics was studied in preterm pigs given pure emulsions. Finally, complete formulas with different emulsions were fed for four days, and the post-bolus plasma triglyceride level was determined. Milk-derived emulsifiers (containing protein and phospholipids from milk fat globule membranes and extracellular vesicles) showed increased effects on fat digestion compared to SL in an in vitro digestion model. Further, milk-derived emulsifiers significantly increased the digestion of triglyceride in the preterm piglet model compared with SL. Ultra-structural images indicated a more regular and smooth surface of fat droplets emulsified with milk-derived emulsifiers relative to SL. We conclude that, relative to SL, milk-derived emulsifiers lead to a different surface ultrastructure on the lipid droplets, and increase lipid digestion.


2021 ◽  
Vol 140 ◽  
pp. 110054
Author(s):  
Pablo Gallego-Lobillo ◽  
Alvaro Ferreira-Lazarte ◽  
Oswaldo Hernández-Hernández ◽  
Mar Villamiel

2021 ◽  
Vol 350 ◽  
pp. 129246
Author(s):  
Serena Martini ◽  
Alice Cattivelli ◽  
Angela Conte ◽  
Davide Tagliazucchi

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