scholarly journals Increasing antioxidant activity of soursop (Annona muricata L.) and noni (Morinda citrifolia L.) leaves fermented by Lactobacillus plantarum BP102

2022 ◽  
Vol 27 (2) ◽  
pp. 73-77
Author(s):  
Dayu Nirwana Putri ◽  
Sri Widyarti ◽  
Yoga Dwi Jatmiko

Free radicals are constantly produced by either cell metabolism or from external sources. At high concentration, they induced a tissue damage called oxidative stress. Soursop leaf (Annona muricata L.) and noni leaf (Morinda citrifolia L.) are medicinal plants with potency as antioxidants. This study aimed to evaluate the capacity of Lactobacillus plantarum BP102 in elevating the antioxidant activity of soursop and noni leaves. Dried-powder and methanol extract of soursop and noni leaves were diluted with sterile distilled water 3 g/30 mL and 0.3 g/30 mL, respectively, inoculated with 1% (v/v) of L. plantarum BP102 inoculum. The antioxidant activity was carried out using the 2.2-diphenyl-1-picrylhydrazyl (DPPH) method. The antioxidant activity increased in dried-powder and methanol extract of soursop and noni leaves with different activity levels after being fermented using L. plantarum BP102 based on IC50. The increasing antioxidant activity in dried-powder of soursop leaves IC50 6.41±0.06 to 0.034±0.01 mg/mL (99.5%) was higher than of the methanol extract IC50 2.78±0.00 to 0.11±0.01 mg/mL (96%). Unfortunately, the effect of fermentation towards noni leaves could only be observed in the form of methanol extract IC50 12.8±0.01 to 0.33±0.02 mg/mL (increased by 97.4%), the dried-powder of noni leaves was suspended and produced a dark color. The probiotic L. plantarum BP102 was used as a fermented agent in increasing the bioactive compounds especially related to antioxidant activity.

Author(s):  
Irfan Zamzani ◽  
Nita Triadisti

Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.


2018 ◽  
Vol 10 (1) ◽  
pp. 276
Author(s):  
Anisa Puspitaningrum ◽  
Berna Elya ◽  
Katrin .

Objective: This study aimed to isolate and characterize the compounds responsible for the high antioxidant activities of the ethyl acetate and methanolextracts of Garcinia daedalanthera Pierre leaves.Methods: In this study, the ethyl acetate extract was obtained by column chromatography, and the methanol extract was obtained by vacuum columnchromatography. The mobile phase comprised n-hexane, ethyl acetate, and methanol with increased polarity. Antioxidant activity was examined usingthe 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The fraction with the highest antioxidant activity was purified through column chromatography,recrystallization, and preparative thin-layer chromatography. This fraction, termed the isolate of B, was identified using DPPH and AlCl3, and itsantioxidant activity was quantitatively tested.Results: From this research, 21.7 mg of the isolate of B were obtained with an IC50 of 5.82 μg/mL. Identification using an AlCl3 sprayer producedyellow phosphorescent spots under UV light. UV-Vis spectrum analysis revealed the presence of an aromatic compound and conjugated double bonds.Infrared spectrum analysis revealed the presence of −OH, C–H alkane, C=C aromatic, C=O, and C-O-C groups.Conclusion: Based on this research, 21.7 mg of the isolate of B was derived through fractionation of the methanol extract, and this isolate exhibitedantioxidant activity with an IC50 of 5.82 μg/mL. The isolate of B was considered to be a flavonoid, as it was fluorescent under UV light (366 nm) afterbeing sprayed with AlCl3 reagent.


2017 ◽  
Vol 2 (1) ◽  
pp. 133
Author(s):  
Satria Bagus Firmansyah ◽  
R. Arizal Firmansyah ◽  
Nur Hayati

<p style="text-align: justify;">The research activity of the antioxidant and antibacterial esktrak methanol seaweed Sargussum duplicatum J. Agardh as well as its potential as an alternative natural preservative salted egg has been done. Seaweed extract S. duplicatum Jj. Agardh assayed phytochemistry and the content of total phenolic with variation of temperature. Metabolite secondary in seawed extract obtained through maseration using methanol solvent. The extract are partitioned, R1 and R2. R1 sample is a sample without treatment and sample R2 is a sample with 45 minutes for warming treatment in waterbath temperature to 100 °C. Phytochemical content in seawed of S. duplicatum J. Agardh are flavonoid dan steroid. Phytochemical content and phenolic total have positive correlation to antioxidant activity. Antioxidant activity with DPPH method yielded IC50 = 143.03 μg/mL (R1) and 357.95 μg/mL (R2). Antibacterial activity assay carried out trough diffusion method with SSA media (Salmonella-Shigella Agar). Inhibition value toward Salmonella is 1.120 mm and 1.15 mm with control is chloramphenicol. Salmonella sp.is pathogenic bacteria that presents in egg and causes decay on the egg. Discussion about antibacteria activity results of seawed extract of S. duplicatum J. Agardh are suggested to apply toward egg preservation method. ©2016 JNSMR UIN Walisongo. All rights reserved</p>


2019 ◽  
Vol 2 (2) ◽  
pp. 79-83
Author(s):  
Dela Rosa ◽  
Catherine Roeroe ◽  
Agustina Susanti

In this research the potential of Pakoba leaf extract to be used as antioxidant and skin whitening (anti-melanogenic agent) is investigated. The antioxidant activity of Pakoba leaves were studied using DPPH method and the result showed that the 80 percent methanol crude extract has strong antioxidant activity with an IC50 value of 22.66 ± 1.02 μg/ml. The aqueous fraction of the sample has an IC50 value of 53.30 ± 1.42 μg/ml, followed by n-butanol fraction (53.63 ± 1.45 μg/ml) and chloroform fraction (511.54 ± 1.59 μg/ml). The anti-melanogenic activity of the crude methanol extract showed IC50 value of 316.56 ± 11.04 μg/ml. Thus, it isconcluded that crude extract of Pakoba leaves shows good potential as the antioxidant source although it does not show good anti- melanogenic activity.


EKOLOGIA ◽  
2020 ◽  
Vol 19 (1) ◽  
pp. 34-38
Author(s):  
Siti Warnasih ◽  
Diana Widiastuti ◽  
Uswatun Hasanah ◽  
Laksmi Ambarsari ◽  
Purwantiningsih Sugita

Date seeds are waste from palm fruit processing that has not been utilized optimally. Date seeds contain flavonoids which are known to act as antioxidants. The aim of this study was to determine the antioxidant activity and flavonoid of methanol extract which was carried out by soxhletation and its fractionation results. Date seeds are made into simplicia, soxhlet extraction with methanol, then the methanol extract is fractionated in stages by liquid-liquid partition with n-hexane, ethyl acetate, and n-butanol. Each fraction and extract was determined by its antioxidant activity with the DPPH method and its flavonoid was determined by spectrophotometry. Ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 5.74 ± 0.05μg/mL, followed by methanol extract of 9.55±0.53 μg/mL, n-butanol fraction 19.73±0,58 μg/mL, and n-hexane fraction of 289.59±10.52 μg/mL, while the IC50 value for vitamin C as a positive control was 4.29±0.74 μg/mL. The highest flavonoids were produced from ethyl acetate fractions which amounted to 1484.33 ± 161.47 mg Quercetin Equivalent (QE) / 100 g, followed respectively by methanol extract of 282.84±13.72 mg QE/100 g.


Author(s):  
Krishma M. Jadav ◽  
K. N. Ninge Gowda

Objective: Four different extracts of Araucaria columnaris (bark peel) and Cosmos sulphureus (flowers) were screened for their phytochemical composition, and free radical scavenging activities.Methods: DPPH method was used to test the antioxidant activity for extracts.Results: Among the different extracts tested, the methanol extract of both the plant species showed significant radical scavenging activities. Phytochemical analysis of the extracts revealed that the radical scavenging activities might be due to the presence of flavonoids, tannins and phenolic compounds.Conclusion: The results obtained suggest that Araucaria columnaris (bark peel) and Cosmos sulphureus(flowers) could be exploited in the treatment of various diseases like cancer, cardiovascular diseases and infection diseases.  


Author(s):  
FONA QORINA ◽  
ADE ARSIANTI ◽  
QOTRUNNADA FITHROTUNNISA ◽  
NADZILA ANINDYA TEJAPUTRI

Objective: Soursop (Annona muricata) is a tropical plant which has been utilized as a folk medicine to treat many diseases including cancer, inflammation and parasitic infection. In this study, we investigated its phytochemistry properties and antioxidant activity against free radicals. Methods: Annona muricata leaves were extracted in three different solvents: ethanol, ethyl acetate and n-hexane. Afterwards, a phytochemistry test and the thin layer chromatography (TLC) method were used to evaluate bioactive compounds contained in the three different extracts. Antioxidant activity from the semi-polar (ethyl acetate) and polar (ethanol) solvents were evaluated by the DPPH (1,1-diphenyl-2-picrylhydrazyl) method, and the antioxidant activity is expressed by IC50 value. The results were compared to ascorbic acid as a positive control. Results: The phytochemistry test showed that the extracts were positive for flavonoids, steroids, alkaloids, glycosides and tannins. Moreover, TLC analysis revealed that there were three chemical compounds contained in the extracts. The results from the DPPH method were that ethanolic extract was shown to have the most potent antioxidant activity with an IC50 value of 35.51 ppm. Conclusion: The ethanolic extract of Annona muricata could be developed as the next promising natural antioxidant source.


2018 ◽  
Vol 1 (2) ◽  
pp. 161 ◽  
Author(s):  
Indah Purwaningsih ◽  
Reskiya Sapriani ◽  
Ratih Indrawati

Abstract: Kesum Plants (Polygonum minus) is one of the endemic plants in West Borneo containing phenolic compounds, steroids, flavonoids, alkaloids, tannins, saponins, and beta-carotene. Phenolic, flavonoids and beta-carotene are antioxidant compounds. Most phenolic and flavonoid groups are polar compounds. Therefore, in this research used methanol solvent as representative of polar solvent. The purpose of this research  was to know the difference of antioxidant activity of methanol extract of kesum leaves with vitamin C. The extraction  is done by maseration for 3 x 24 hours, where the solvent is changed every 24 hours. The method used to measure antioxidant activity is the DPPH method (2,2-diphenyl-1-picrylhydrazyl). The samples used in this paper are methanol extract of  kesum leaves in 80, 65, 50, 35, and 20 ppm with five replications. The comparator used is vitamin C. The result of the research showed that IC50 of methanol extract was 20,632 ppm and IC50 of vitamin C 6,175 ppm. Based on the results of this study it can be concluded that vitamin C and methanol extract of serum leaves have a very strong antioxidant activity.Abstrak: Tanaman Kesum (Polygonum minus) merupakan salah satu tanaman endemik di wilayah Kalimantan Barat yang mengandung senyawa golongan fenolik, steroid, flavonoid, alkaloid, tanin, saponin, dan betakaroten. Golongan fenolik, flavonoid dan betakaroten merupakan senyawa antioksidan. Sebagian besar golongan fenolik dan flavonoid merupakan senyawa polar. Maka dari itu, pada penelitian ini digunakan pelarut metanol sebagai perwakilan pelarut polar. Tujuan penelitian ini untuk mengetahui perbedaan aktivitas antioksidan ekstrak metanol daun kesum terhadap vitamin C. Proses ekstraksi dilakukan dengan cara maserasi selama 3 x 24 jam, dimana pelarutnya diganti setiap 24 jam sekali. Metode yang digunakan untuk mengukur aktivitas antioksidan adalah metode DPPH (2,2-difenil-1-pikrilhidrazil). Sampel yang digunakan pada penelitian ini yaitu ekstrak metanol daun kesum konsentrasi 80, 65, 50, 35, dan 20 ppm dengan pengulangan sebanyak lima kali. Pembanding yang digunakan adalah vitamin C. Hasil penelitian didapatkan IC50 ekstrak metanol daun kesum sebesar 20.632 ppm dan IC50 vitamin C sebesar 6.175 ppm. Berdasarkan hasil penelitian dapat disimpulkan bahwa vitamin C dan ekstrak metanol daun kesum memiliki aktivitas antioksidan yang sangat kuat.


2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Miguel Angel De los Santos-Santos ◽  
Rosendo Balois-Morales ◽  
José Orlando Jiménez-Zurita ◽  
Irán Alia-Tejacal ◽  
Graciela Guadalupe López-Guzmán ◽  
...  

The soursop fruit (Annona muricata L.) is a crop of significant economic value for Nayarit, which is characterized by having a bittersweet taste, making it attractive to the consumer. However, the soursop has rapid maturation which causes a short shelf life. Several postharvest management techniques have been applied to reduce its metabolic processes, such as refrigeration, use of 1-methylcyclopropene (1-MCP), and controlled and modified atmospheres. In recent years, polysaccharide-based coatings have been applied to fruits. Therefore, the objective of this investigation was to evaluate the physicochemical and biochemical changes, as well as the antioxidant activity of soursop fruits with a mucilage-based coating (2%), stored at 22°C and 15°C with a 90% RH. Weight loss, firmness, color, soluble solids, acidity, pH, phenols, flavonoids, vitamin C, and antioxidant activity were evaluated. The results obtained in the coated fruits stored at 15°C showed lower weight loss (6.4%), lower firmness (29.7 N), higher TSS concentration (10.4°Bx), and lower acidity (0.38%) compared with the uncoated fruits. The total phenolic content decreased in coated fruits stored at 22°C (54.3 mg EGA/100 g FW). The highest antioxidant activity (DPPH method) was recorded in fruits coated and stored at 15°C with an average value of 257.9 mg EAA/100 g FW. Moreover, a high concentration of vitamin C was observed in fruits coated and stored at 15°C and 22°C (20.5 and 17.5 mg EAA/100 g FW), concluding that the coating based on roselle mucilage (2%) in combination with a temperature of 15°C prevents weight loss, decreases titratable acidity, and increases the content of phenols and vitamin C. Furthermore, an increase in the shelf life up to eight days and in the antioxidant activity at the maturity of consumption was observed in the fruits coated with 2% roselle mucilage stored at 15°C.


2021 ◽  
Vol 8 (1) ◽  
pp. 94
Author(s):  
Dwi Rizki Febrianti ◽  
Rakhmadhan Niah ◽  
Novia Ariani

Daun Kumpai Mahung (Eupathorium inulifolium H.B & K) merupakan salah satu tumbuhan endemik Kalimantan Selatan. Secara turun temurun digunakan sebagai obat tradisional Dayak Meratus sebagai obat diare, demam, dan malaria. Tanaman ini dicurigai memiliki nilai antioksidan tinggi karena mengandung metabolit skunder fenolik. Tujuan penelitian ini untuk mengetahui aktivitas antioksidan ekstrak metanol daun E. inulifolium serta nilai IC50-nya. Penelitian ini menggunakan metode DPPH dengan instrumen spektofotometri UV-vis dengan panjang gelombang 517 nm. Dari hasil perhitungan dan replikasi nilai IC50 yang didapat sebesar 38,9 ppm. Ekstrak daun E. inulifolium memiliki aktivitas antioksidan yang sangat kuat dalam meredam radikal bebas. Kata Kunci: Daun, Potensi Antioksidan, Endemik, IC50, Ekstrak Etanol, Fenolik Kumpai Mahung (Eupathorium inulifolium H.B & K) leaves are one of the endemic plants of South Kalimantan. From generation to generation it is used as a traditional medicine for Dayak Meratus as a medicine for diarrhea, fever, and malaria. This plant is suspected of having high antioxidant value because it contains phenolic secondary metabolites. The purpose of this study was to determine the antioxidant activity of the methanol extract of E. inulifolium leaves and its IC50 value. This study used the DPPH method with spectophotometer UV-vis instrument at wavelength of 517 nm. From the calculation and replication, the IC50 value obtained is 38.9 ppm. E. inulifolium leaf extract has very strong antioxidant activity in reducing free radicals. 


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