scholarly journals Determination of Antimicrobial Susceptibility Patterns between Different Strains of Propionibacterium Acnes

2019 ◽  
Vol 4 (06) ◽  
Author(s):  
Yusufu, W. N. ◽  
Suleiman, H. O. ◽  
Akwa, V. Y. ◽  
David, D. L. ◽  
Taiga, A.
Keyword(s):  
Antimicrobial Susceptibility ◽  
Propionibacterium Acnes ◽  
Acne Vulgaris ◽  
Antibiotics Resistance ◽  
No Inhibition ◽  
Zones Of Inhibition ◽  
Weak Activity ◽  
Inhibition Zones ◽  
Different Strains ◽  
Susceptibility Patterns

Propionibacterium acnes (P. acnes) isolated in infections of deep tissues was originally thought to be as a result of improper sterilization of skin and hence contamination of medical processes with this commensal/ normal flora of the skin. P. acnes has latter been identified as the principal agent responsible for the progression of some deep tissue diseases. The antimicrobial susceptibility patterns between different strains of P. acnes was determined. Ten (10) P. acnes clinical isolates of infections were considered, two (2) from acne vulgaris and eight (8) [two (2) per recA types 1A1, 1B, II and III] from lumber herniation tissues. Antibiotic susceptibility of some antibiotics was also determined and resistance to tetracycline was seen in 1 isolate, trimethoprim (1 isolate) and weak activity seen in 5 isolates. Heteroresistance to rifampicin was witnessed among 6 isolates. Upon four days of incubation, no inhibition of the bacterial growth by tetracycline in isolate 1(III). Isolate 17(1B) had no inhibition from trimethoprim. There was low inhibition of P. acnes by this antibiotic in isolates 71(IA1), 82(IB), 55(II), 1 (III) and 64(III). Amoxicillin and rifampicin created greater zones of inhibition among P. acnes isolates. Erythromycin was the next most inhibiting antibiotic with greater inhibition zones. Although Rifampicin had large inhibition zones, heteroresistance was observed among isolates 82(IB), 24(II), I (III), 17(IB), 55(II) and 71(IA1) as colonies were observed growing within the zones after 4 days of incubation. The antibiotics resistance expressed in the study were seen displayed among the lumber disc herniation isolates only with none involving acne vulgaris isolates. This study supports the theory that most P. acnes isolates in deep tissues exhibit higher rate of antibiotic resistance. P. acnes hence might be named the etiological agent of foreign-body infections like infections of indwelling medical devices.

scholarly journals In vitro antimicrobial susceptibility patterns of Propionibacterium acnes isolated from patients with acne vulgaris

2016 ◽  
Vol 10 (10) ◽  
pp. 1140-1145 ◽  
Author(s):  
Indu Biswal ◽  
Rajni Gaind ◽  
Neeraj Kumar ◽  
Srujana Mohanty ◽  
Vikas Manchanda ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Propionibacterium Acnes ◽  
Clinical Laboratory ◽  
Acne Vulgaris ◽  
Brain Heart Infusion ◽  
Tetracycline Resistance ◽  
Agar Dilution ◽  
Resistance Rates ◽  
Systemic Antibiotics

Introduction: Propionibacterium acnes has been implicated in the development of acne vulgaris. Rampant use of topical and systemic antibiotics for acne vulgaris has led to resistance due to selective pressure. This study aimed to determine antibiotic resistance of P. acnes. Methodology: A total of 102 samples were collected from acne lesions and cultured onto sheep’s blood agar and brain-heart infusion agar supplemented with 5 g/L glucose and 2 mg/L furazolidone) (BHIg) under aerobic and anaerobic conditions. Species identification was done by conventional methods and the VITEK2 Compact system. The isolates were tested for penicillin, erythromycin, clindamycin, ciprofloxacin, nadifloxacin, and tetracycline by E-test, and minimum inhibitory concentration (MIC) of minocycline was determined by agar dilution on BHIg. MIC results were interpreted as per EUCAST (European Committee on Antimicrobial Susceptibility Testing) and CLSI (Clinical Laboratory Standards Institute) guidelines. Results: P. acnes was the most common anaerobe (66%) isolated. Resistance rates using EUCAST and CLSI breakpoints were 10.6% and 6.1%, 7.6% and 0%, 7.8% and 0% for erythromycin, clindamycin, and minocycline, respectively. Tetracycline resistance was observed in 9.2% isolates irrespective of the interpretative criteria used. MIC50 and MIC90 values for nadifloxacin (0.25 and 1 µg/mL) were found to be twofold lower than those for ciprofloxacin (0.5 and 1 µg/mL). Similarly, MIC50 and MIC90 values for minocycline (0.125 and 0.5 µg/mL) were also two- to threefold lower than those for tetracycline (0.38 and 1 µg/mL). Conclusions: To the best of our knowledge, this is the first study focusing on P. acnes resistance from India.

scholarly journals Antimicrobial activity of three medicinal plants against acne-inducing bacteria Propionibacterium acnes

2018 ◽  
Vol 5 (10) ◽  
pp. 277-288
Author(s):  
S. E. Abiya ◽  
B. O. Odiyi ◽  
L. R. Falarunu ◽  
N. U. Abiya
Keyword(s):  
Medicinal Plants ◽  
Propionibacterium Acnes ◽  
Acne Vulgaris ◽  
Aloe Vera ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Zones Of Inhibition ◽  
Synthetic Standard

The antimicrobial activities of three medicinal plants (Senna alata L., Azadirachta indica A. Juss, and Aloe vera (L.) Burm.f.) against pimples causing bacterium Propionibacterium acnes was studied using disc diffusion method. Extracts from each plant were used on the bacterium at three different dosage concentrations (0.1 mL, 0.15 mL and 0.2 mL). Their Zone of inhibition was measured in millimeter (mm) and compared against a known synthetic standard (Gentamycin). Results indicate that the plants differ significantly in their activity against the studied microorganism. S. alata had the highest inhibitory effect of all the plants used (26.00 mm, 30.67 mm and 36.00 mm, for 0.1 mL, 0.15 mL and 0.2 mL dosage concentration, respectively). This was followed by A. indica with 9.33 mm, 15.67 mm and 16.67 mm zone of inhibition for 0.1 mL, 0.15 mL and 0.2 mL dosage concentration, respectively. A. vera had no effect (0.0 mm zone of inhibition) at 0.1 mL and 0.15 mL dosage concentrations, but at 2.0 mL dosage concentration, 4.0 mm zone of inhibition was achieved. Gentamycin showed zones of inhibition of 17.33 mm, 26.67 mm and 22.67 mm, for 0.1 mL, 0.15 mL and 0.2 mL dosage concentration, respectively. A comparison of all result obtained from the three plant extracts and gentamycin shows that S. alata have a significantly higher (p > 0.05) inhibitory effect against the pimples causing bacterium; Propionibacterium acnes than all the other treatments. The trend follows S. alata > Gentamycin > A. indica > A. vera, respectively, in terms of their inhibitory effect. Therefore, S. alata is more active and is the most appropriate plant to be used for treating of acne vulgaris among the three plant species selected for this experiment.

scholarly journals Determination of Biofilm Formation between Different Strains of Propionibacterium acnes Using Biofilm Assay

2019 ◽  
Vol 4 (02) ◽  
Author(s):  
Yusufu, W. N. ◽  
Suleiman, H. O. ◽  
Akwa, V. Y. ◽  
David, D. L. ◽  
Taiga, A.
Keyword(s):  
Biofilm Formation ◽  
Propionibacterium Acnes ◽  
Acne Vulgaris ◽  
Microtiter Plate ◽  
Deep Tissue ◽  
Microtiter Plate Assay ◽  
Biofilm Production ◽  
Culture Isolate ◽  
Different Strains

Propionibacterium acnes (P. acnes) a member of the normal flora of the skin has constantly been associated with deep tissue infections especially during medical processes. P. acnes have been isolated in deep tissues and are believed to be an aetiological agent in these infections, contributing to the progression of some of these diseases. The biofilm formation ability between different strains of P. acnes was determined. Ten (10) P. acnes clinical isolates were considered, two (2) from acne vulgaris and eight (8) [two (2) per recA types 1A1, 1B, II and III] from lumber herniation tissues. Semi quantitative biofilm analysis using the microtiter plate assay was used with some modification. The semi quantitative biofilm assay was done in triplicates. The result obtained from the biofilm triplicates from 4 days’ incubation using 3 days’ culture showed that isolates 17(IB), 82(IB) and 55(II) showed very high biofilm production for 2 replicates which implies that they are real biofilm producing isolates. Using overnight cultures, higher biofilm production was witnessed with isolates 1(III), Lesion 7 and 84 (IA1) being the highest biofilm producers. Although with 3 days’ culture, isolate 1(III) could easily be discarded as a-non biofilm producer, while lesion 7 and 84 (IA) has been associated to biofilm formation in 3 days’ culture. The production of biofilms by isolates supports the theory that the ability of P. acnes to form biofilms enables it to attach to medical implants hence causing deep tissue infections.

scholarly journals Formulasi Sediaan Krim Anti Acne dan Uji Aktivitas Ekstrak Etanol Bawang Dayak (Eleutherine bulbosa (Mill.) Urb) terhadap Propionibacterium acnes

2019 ◽  
Vol 2 (2) ◽  
pp. 121-126
Author(s):  
Syahrida Dian Ardhany ◽  
Yunari Puspitasari ◽  
Yuyun Meydawati ◽  
Susi Novaryatiin
Keyword(s):  
Propionibacterium Acnes ◽  
Acne Vulgaris ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Homogeneity Test ◽  
Moderate Activity ◽  
Adhesion Test ◽  
Physical Test ◽  
Weak Activity ◽  
Dispersion Test

Bawang dayak (Eleutherine bulbosa (Mill.)urb) is one of plants of Central Kalimantan. Empirically, Bawang dayak is used to treat skin problems, one of them is acne vulgaris, so that it is necessary to test the inhibition of ethanolic extract of Bawang Dayak against acne causing such as P.acnes.  In the pharmaceutical world there is still no research conducted on the manufacture of antiacne cream contain ethanolic extract of Bawang Dayak bulbs, so the researchers conducted it to improve the efficiency of the use of traditional medicine. The results of phytochemical screening showed that ethanolic extract of bawang dayak contained flavonoid, alkaloid, saponin, tanin and catechol with the inhibition zone classified as moderate activity at the concentration of 1% and weak activity at other concentrations (5%, 10% and 15%). Based on the evalution of cream preparations the results of the organoleptic test for antuacne cream have a dark brown color and distinctive odor, homogen for homogeneity test, adhesion test and dispersion test according to the requirements and the pH observation showed ph (6) suitable for topical application. Based on these results, the evaluation antiacne cream fulfills the physical test, but it needs to be developed further for stability and effectiveness of antiacne cream against the bacteria acnes causing.   

Citrula Gel dari Limbah Kulit Buah Semangka (Citrullus lanatus (Thunb.) Matsum & Nakai) sebagai Antijerawat (Acne vulgaris)

2019 ◽  
Vol 16 (1) ◽  
pp. 74
Author(s):  
Yola Anggraeni ◽  
Tika Ambarwati ◽  
Irmas Miranti ◽  
Erza Genatrika
Keyword(s):  
Staphylococcus Aureus ◽  
Propionibacterium Acnes ◽  
Citrullus Lanatus ◽  
Acne Vulgaris ◽  
Carbopol 940

Jerawat  didefinisikan  sebagai  peradangan  kronik  dari  folikel  polisebasea  yang disebabkan  oleh  beberapa  faktor  dengan  gambaran  klinis  yang  khas.  Salah  satu tanaman  yang  dapat  mengatasi  jerawat  yaitu semangka.  Kulit buah semangka (Citrullus lanatus (Thunb.) Matsum & Nakai) memiliki   senyawa antibakteri di antaranya  alkaloid,  fenol,  saponin,  dan  terpenoid.  Dalam penelitian ini ekstrak limbah  kulit  buah semangka diformulasikan   dalam   sediaan   gel.   Tahap   penelitian   yang menggunakan  rancangan  acak  lengkap ini meliputi  penyiapan  dan  pengumpulan simplisia   limbah   kulit   buah   semangka,   ekstraksi,   uji kandungan senyawa, formulasi gel, evaluasi sifat fisik sediaan gel, dan uji aktivitas antibakteri  terhadap  bakteri  Propionibacterium acnes  dan  Staphylococcus  aureus. Ekstraksi yang dilakukan adalah dengan menggunakan metode maserasi dengan penyari kloroform. Uji kandungan senyawa dilakukan dengan menggunakan metode penapisan fitokimia meliputi uji alkaloid, uji triterpenoid, uji fenol, dan uji saponin. Ekstrak kulit buah semangka dengan konsentrasi 5, 10, dan 15% kemudian diformulasikan dengan carbopol 940 dan dilanjutkan dengan evaluasi sifat fisik gel, di antaranya uji organoleptis, uji homogenitas, uji daya sebar, uji daya lekat, uji pH, dan uji viskositas. Uji aktivitas antibakteri dari formulasi ekstrak kulit buah semangka dilakukan dengan menggunakan metode difusi agar. Hasil formulasi terbaik pada formulasi gel menghasilkan gel yang homogen, lekat, menyebar, pH yang cocok dengan kulit, dan memiliki viskositas yang cukup baik. Hasil terbaik dari zona hambat uji antibakteri pada bakteri Propionibacterium acnes yaitu 5,23 mm dan pada bakteri Staphylococcus  aureus yaitu 5,80 mm.

FORMULATION AND CHARACTERIZATION OINMENT OF ARUM MANIS MANGO (Mangifera indica L.) LEAVES METHANOL EXTRACTS AS AN ANTIBACTERIAL AGAINST Propionibacterium acnes

2020 ◽  
Vol 16 ◽  
Author(s):  
Dian Riana Ningsih ◽  
Zusfahair Zusfahair ◽  
Dadan Hermawan ◽  
Wulan Anggraeni ◽  
Hassan Y. Aboul-Enein
Keyword(s):  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Methanol Extract ◽  
Mangifera Indica ◽  
Inhibition Zone ◽  
Sweat Glands ◽  
Ointment Formulation ◽  
Inhibition Zones ◽  
Mango Leaves ◽  
Dispersive Power

Background: Acne is caused by several factors including the active secretion of sebaceous sweat glands, hyperkeratosis in the hair infundibulum and the effects of bacteria. One of the plants that has the potential as an antibacterial is the extract of arumanis mango leaves. Method: Determine the Minimum Inhibitory Concentration (MIC) of methanol extract of mango leaves which can inhibit Propionibacterium acnes activity. The antibacterial activity tests were performed using agar diffusion. The ointment formulation, the characteristics of ointment preparations and the ointment activity against P. Acnes are discussed. Result: MIC of methanol extract of mango leaves value is 5 ppm with an inhibition zone of 1 mm. The ointment obtained is white, has distinctive smell, semisolid form, possesses a pH of 4.92 - 5.87, dispersive power of 5.05 - 6.30 cm, adhesive power of 1 - 3.67 seconds, homogeneous and protective. Ointment preparations of methanol extract of mango leaves has activity on P. acnes on the 0 and 15th day of storage. The activities of ointment preparation on day 0 with concentrations of 0, 5, 10 and 15 ppm are 0.00 mm; 10.20 mm; 19.97 mm and 23.60 mm respectively, while the inhibition zones produced by the preparation of ointment on day 15 with concentrations of 0, 5, 10 and 15 ppm are 0.00 mm; 5.71 mm; 9.58 mm and 21.88 mm respectively. Conclusion: Methanol extract of mango leaves (Mangifera indica L.) and oinment preparation are able to inhibit the growth of Propionibacterium acnes.

scholarly journals Bacterial profile and antimicrobial susceptibility patterns of isolates among patients diagnosed with surgical site infection at a tertiary teaching hospital in Ethiopia: a prospective cohort study

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Gemedo Misha ◽  
Legese Chelkeba ◽  
Tsegaye Melaku
Keyword(s):  
Escherichia Coli ◽  
Cohort Study ◽  
Surgical Site Infection ◽  
Prospective Cohort Study ◽  
Antimicrobial Susceptibility ◽  
Prospective Cohort ◽  
Site Infection ◽  
Gram Negative ◽  
Tertiary Teaching ◽  
Susceptibility Patterns

Abstract Background Globally, surgical site infections are the most reported healthcare-associated infection and common surgical complication. In developing countries such as Ethiopia, there is a paucity of published reports on the microbiologic profile and resistance patterns of an isolates. Objective This study aimed at assessing the bacterial profile and antimicrobial susceptibility patterns of isolates among patients diagnosed with surgical site infection at Jimma Medical Center in Ethiopia. Methods A prospective cohort study was employed among adult patients who underwent either elective or emergency surgical procedures. All the eligible patients were followed for 30 days for the occurrence of surgical site infection (SSI). From those who developed SSI, infected wound specimens were collected and studied bacteriologically. Results Of 251 study participants, 126 (50.2%) of them were females. The mean ± SD age of the patients was 38 ± 16.30 years. The overall postoperative surgical site infection rate was 21.1% and of these 71.7% (38/53) were culture positive. On gram stain analysis, 78% of them were Gram-negative, 11.5% were Gram-positive and 10.5% were a mixture of two microbial growths. Escherichia coli accounted for (21.43%), followed by Pseudomonas aeruginosa (19.05%), Proteus species (spp.) 14.29%), Staphylococcus aureus (11.90%), Klebsiella species (11.90%), Citrobacter spp. (9.5%), streptococcal spp. (7.14%), Coagulase-negative S. aureus (CoNS) (2.38%) Conclusion Gram-negative bacteria were the most dominant isolates from surgical sites in the study area. Among the Gram-negative bacilli, Escherichia coli were the most common bacteria causing surgical site infection. As there is high antibiotic resistance observed in the current study, it is necessary for routine microbial analysis of samples and their antibiogram.

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