CHARACTERISTIC OF ANAMNESIS AND LABORATORY PARAMETERS OF CHILDREN WITH PLATELET PATHOLOGY

2021 ◽  
Vol 100 (3) ◽  
pp. 47-53
Author(s):  
M.M. Tсvetkova ◽  
◽  
L.M. Minkina ◽  
O.E. Aliyeva ◽  
A.A. Kravchuk ◽  
...  
Keyword(s):  
Viral Load ◽  
Children And Adolescents ◽  
Von Willebrand Factor ◽  
Medical Records ◽  
Relative Number ◽  
Immune Protection ◽  
Single Center ◽  
Compensatory Mechanisms ◽  
Von Willebrand ◽  
Willebrand Factor

The aim of the study is to identify the features of the anamnesis and laboratory characteristics of children and adolescents with platelet pathology. Materials and methods of research: the features of medical history and laboratory characteristics of children and adolescents aged 1 to 17 years with platelet pathology (thrombocytopenia and thrombocytopathy) were studied based on the analysis of 112 medical records of inpatients. Study design: retrospective, single-center, solid, non-randomized, uncontrolled. Results: when comparing parameters of the indicated nosological groups, a higher viral load was revealed in children with thrombocytopenia (level of antibodies to the herpesvirus group was statistically significantly higher in children with thrombocytopenia than with thrombocytopathy: for HSV1,2 and VEB – p<0,001, CMV – p=0,008). According to hemostasiogram, APTT was statistically significantly higher in the thrombocytopathy group (29,56±2,18 s versus 28,44±1,62 s respectively, p=0,0011), the prothrombin time was higher in thrombocytopenia (12,37±0,72 s and 12,03±0,48 s, respectively, p=0,014). The activity of von Willebrand factor is statistically significantly higher in thrombocytopenia compared with thrombocytopathy (123,78±36,35% versus 79,73±35,21%, respectively, p<0,001). A positive correlation between the relative number of platelets and leukocytes (Rs=0,40, p<0,001) has been established in the group of children with thrombocytopathy. Conclusion: the differences and dependencies identified indicate the inclusion of compensatory mechanisms of hemostasis regulation and immune protection in platelet pathology, which is more pronounced with decrease in their number.

scholarly journals Elevated plasma von Willebrand factor levels are associated with subsequent ischemic stroke in persons with treated HIV infection

2021 ◽  
Author(s):  
Susan M Graham ◽  
Robin M Nance ◽  
Junmei Chen ◽  
Jennie Le ◽  
Dominic W Chung ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Viral Load ◽  
Hiv Infection ◽  
Von Willebrand Factor ◽  
Endothelial Activation ◽  
Apolipoprotein A1 ◽  
Soluble Cd14 ◽  
Amyloid A ◽  
Von Willebrand ◽  
Willebrand Factor

Abstract Background We assessed whether key biomarkers of endothelial activation and hemostasis/thrombosis were elevated in individuals receiving effective antiretroviral therapy (ART) in the year before ischemic stroke. Methods We conducted a case-control study nested in the CNICS cohort, comparing 42 adjudicated cases with ischemic stroke to 83 controls matched for ART regimen. Angiopoietin-1, angiopoietin-2, C-reactive protein, interleukin-6, plasminogen activation inhibitor-1, P-selectin, serum amyloid-A, soluble CD14, ICAM-1, VCAM-1, apolipoprotein A1, ADAMTS13, and von Willebrand factor (VWF) were measured in stored plasma collected before the stroke event. We used conditional logistic regression to identify associations with ischemic stroke, with and without adjustment for Atherosclerotic Cardiovascular Disease (ASCVD) and Veterans Aging Cohort Study (VACS) scores. Results After adjustment for age and sex, higher plasma viral load and higher angiopoeitin-2, soluble CD14, and VWF were associated with increased odds of ischemic stroke; higher nadir CD4 count was associated with decreased odds of ischemic stroke. VWF remained associated with subsequent ischemic stroke after adjustment for ASCVD score (adjusted odds 1.74, 95%CI 1.01–2.98 per log2 increment). In a separate model adjusting for VACS score, only VWF (adjusted odds 1.80, 95% CI 1.04–3.12 per log2 increment) was associated with subsequent ischemic stroke. In a sensitivity analysis excluding participants with viral load ≥400 copies/mL, associations between VWF and ischemic stroke were attenuated, with risk estimates ranging from 1.59–1.64 per log2 increment. Conclusions Endothelial activation and related release and attachment of VWF may play an important role in ischemic stroke among persons living with treated HIV infection.

Association of ABO(H) and I blood group system development with von Willebrand factor and Factor VIII plasma levels in children and adolescents

Transfusion ◽  
2010 ◽  
Vol 50 (7) ◽  
pp. 1571-1580 ◽  
Author(s):  
Dieter Klarmann ◽  
Christine Eggert ◽  
Christof Geisen ◽  
Sabine Becker ◽  
Erhard Seifried ◽  
...  
Keyword(s):  
Factor Viii ◽  
Children And Adolescents ◽  
Blood Group ◽  
Von Willebrand Factor ◽  
Plasma Levels ◽  
System Development ◽  
Blood Group System ◽  
Group System ◽  
Von Willebrand ◽  
Willebrand Factor

Visualization of von Willebrand Factor Multimers by Enzyme-Conjugated Secondary Antibodies

1986 ◽  
Vol 55 (02) ◽  
pp. 276-278 ◽  
Author(s):  
F Brosstad ◽  
Inge Kjønniksen ◽  
B Rønning ◽  
H Stormorken
Keyword(s):  
Von Willebrand Factor ◽  
Chromogenic Substrate ◽  
Agarose Electrophoresis ◽  
Secondary Antibodies ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Beta Galactosidase

SummaryA method for visualization of the multimeric forms of von Willebrand Factor (vWF) in plasma and platelets is described. The method is based upon: 1) Separation of the vWF multimers by SDS-agarose electrophoresis, 2) Subsequent blotting of the vWF multimers onto nitrocellulose, 3) Immunolocalization and visualization of the vWF pattern by the sequential incubation of the blot with a) primary vWF antiserum, b) peroxidase- or beta-galactosidase-conjugated secondary antibodies and a relevant chromogenic substrate.

Sign in / Sign up

Export Citation Format

Share Document