scholarly journals Study the Inhibitory Effect of Garlic Extract on S. aureus and E. coli of MultiResistance to Antibiotics.

2015 ◽  
Vol 9 (1) ◽  
pp. 57-61
Author(s):  
Batool Hazim Al-Daherie Al-Daherie ◽  
Oruba Khalid Abbas Abbas
Keyword(s):  
Staphylococcus Aureus ◽  
Multidrug Resistance ◽  
Antibacterial Effect ◽  
Inhibitory Effect ◽  
Garlic Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Ofstaphylococcus Aureus ◽  
Disc Diffusion Method ◽  
E Coli

This Study aimed to investigate the effect of garlic extract against multidrug resistance ofStaphylococcus aureus and E. coli isolated from urine. Isolates were identified by usingbiochemical tests. The susceptibility of these microorganisms towards the most effectiveantibiotics were studied by using disc diffusion method. The multidrug resistancemicroorganisms were challenged with garlic extract using serial dilutions and the technology ofELISA spectrophotometer. The results of this study showed that ten isolates of E. coli and eightisolates of Staphylococcus aureus out of sixty isolates were resistant to all antibiotics. Garlicextract significantly inhibits the growth of these bacteria in a concentration independent manner.This study showed that garlic extract exerts a promising antibacterial effect against multidrugresistance of Staphylococcus aureus and E. coli.

Note: Inhibition of Pathogenic Bacteria by Essential Oils at Different Concentrations

2003 ◽  
Vol 9 (2) ◽  
pp. 85-88 ◽  
Author(s):  
G. Özkan ◽  
O. Sağdiç ◽  
M. Özcan
Keyword(s):  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antibacterial Agents ◽  
Antimicrobial Effect ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Paper Disc

The antimicrobial effect of 11 selected Turkish spice essential oils was investigated against seventeen pathogenic bacteria. The antimicrobial activity of the essential oils of six spices (cumin, fennel, laurel, mint, marjoram, oregano, pickling herb, sage, savory, thyme (black) and thyme) was tested at four concentrations (0.2, 0.4, 1 and 2%) on various microorganisms ( E. aerogenes, E. coli, E. coli O157:H7, K. pneumoniae, P. vulgaris, S. enteritidis, S. gallinarum, S. typhimurium, S. aureus, Y. enterocolitica, A. hydrophila, C. xerosis, M. luteus, M. smegmatis, E. feacalis, P. aeruginosa and P. fluorescens). All preparations showed antibacterial activity against at least one or more bacteria. The inhibitory effect of the essential oils was evaluated through paper disc diffusion method. In general, the essential oils at 1 and 2% levels were effective. The most active essential oils were marjoram, thyme and oregano. According to the results, the studied essential oils potentially might be used as antibacterial agents to prevent the spoilage of food products, although further research is needed.

scholarly journals Antioxidant and antimicrobial effect of alkaloid bulbs extract of Polianthes tuberosa L. (Amaryllidaceae) cultivated in Algeria

2020 ◽  
Vol 10 (4) ◽  
pp. 44-48
Author(s):  
Nihed BARGHOUT ◽  
Nada CHEBATA ◽  
Saida MOUMENE ◽  
Seddik KHENNOUF ◽  
Abdelaziz GHARBI ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Scavenging Effect ◽  
Alkaloid Extracts

Polianthes tuberosa L. (Amaryllidaceae) is an ornamental and medicinal plant. Its flowers and bulbs are used traditionally as a diuretic, emetic, against rashes and gonorrhea. The aim of this work was, to evaluate the antioxidant and antimicrobial activities of bulbs and bulbils alkaloid extracts of P. tuberosa. Antiradical effect was assessed against DPPH radical. However, antimicrobial activity was measured through the disc diffusion method against Escherichae coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Staphylococcus aureus resistant to Methicillin (MRSA) and Candida albicans ATCC 90028. The scavenging effect against DPPH showed that the bulbs and bulbils alkaloids extracts exhibited an antiradical effect with IC50 = 0.231±0.017 mg/mL and 0.233±0.093 mg/mL respectively, less than the effect of vitamin C with IC50 = 0.0194±0.0002 mg/mL. Antimicrobial activity results reveal that both alkaloid bulbs extracts at 50 mg/mL did not have any inhibitory effect against the studied strains using the disc diffusion method. According to this work, bulbs and bulbils alkaloid extracts show a moderate antioxidant effect; that could be recommended as a natural antioxidant. Although tuberose bulbs were used traditionally as a soap substitute; bulbs alkaloid extract has no antimicrobial effect. Keywords: Polianthes tuberosa L., bulbs, bulbils, alkaloids, antiradical activity, antimicrobial activity.

scholarly journals BACTERIOLOGICAL STUDY AND ANTIMICROBIAL SENSITIVITY PATTERN IN CASES OF OSTEOMYELITIS

2020 ◽  
pp. 1-2
Author(s):  
Spriha Smriti ◽  
Babita Kumari ◽  
Pratulya Nandan
Keyword(s):  
Staphylococcus Aureus ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Bacteriological Study ◽  
E Coli ◽  
Antimicrobial Sensitivity ◽  
Common Organism ◽  
Sensitivity Pattern ◽  
Bone Cortex

Introduction- Osteomyelitis refers to infection of bone marrow which spread to the bone cortex and periosteum. Osteomyelitis is most commonly caused by pyogenic bacteria. Despite greater advances in treatment the management of osteomyelitis is quite challenging due to increasing development of antibiotic resistance. Material and Methods -100 patients were evaluated. Samples like pus or exudates or pieces of necrotic tissue were taken. Samples were subjected to gram’s staining and culture. Antimicrobial sensitivity was done using Kirby Bauer disc diffusion method. RESULTS - Staphylococcus aureus was the most common organism isolated followed by E.coli, Klebsiella pneumoniae P. aeuroginosa and Proteus. Staphylococcus aureus + E coli was the most common polymicrobial organism isolated. In trauma cases most common organism isolated was Staphylococcus aureus. Gentamycin was the commonest antibiotic which was effective for treatment of osteomyelitis followed by amikacin, tobramycin and levofloxacin.In cases of gram positive organism max were seen to sensitive to vancomycin and linezolid.

scholarly journals IN VITRO ANTIBACTERIAL EFFECT OF AQUEOUS EXTRACT OF SIDDHARTAKA SNANA USED IN TREATMENT OF VICARCHIKA (ECZEMA)

2021 ◽  
pp. 94-98
Author(s):  
E.R.H.S.S. Ediriweera ◽  
A.M.H.Y Perera ◽  
R. Senavirathne ◽  
R. Rajapaksha
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Skin Diseases ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Cyperus Rotundus ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Vicarchika is a type of Kushta Roga (skin disease). It can be correlated with eczema. Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa are some of the bacteria which cause skin diseases. Siddhartaka Snana is a herbal bath that is described in Charaka Samhita as a treatment for Kushta Roga. Siddhartaka Snana is a herbal preparation which consists ten herbs namely; Cyperus rotundus, Catunaregum spinosa, Phyllanthus emblica, Terminalia chebula, Terminalia bellarica, Cassia fistula, Pongamia pinnata, Holarrhena antidysentrica, Alastonia scholaris and Coscinum fenestratum. Antibacterial effect of Siddhartaka Snana against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa that are responsible for skin diseases was studied using disc diffusion method and well diffusion methods. In disc diffusion method, no inhibition zone was observed with any of the tested bacteria. However, an inhibition zone of 9.67mm was observed against Staphylococcus aureus in well diffusion method with Siddhartaka Snana. Minimum Inhibitory Concentration (MIC) of Siddhartaka Snana against Staphylococcus aureus was 0.9225 mg/1ml. Ingredients of Siddhartaka Snana contain tannins, phenols and flavonoids, all of which possess antibacterial properties. Antibacterial effect of Siddhartaka Snana may have been exhibited due to actions of these phytochemicals. It was concluded that Siddhartaka Snana has an antibacterial effect against Staphylococcus aureus.

scholarly journals What about the Antibacterial Activity of Fresh Ginger Juice (Zingiber officinale)?

2021 ◽  
pp. 163-172
Author(s):  
E. Günaydın ◽  
Gülşen Goncagül ◽  
Çiğdem Güceyü ◽  
Ö. Kardoğan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Intensive Care ◽  
Antibacterial Effect ◽  
Zingiber Officinale ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Antibacterial Effects ◽  
Disc Diffusion Method

Background: The aim of this study was to determine the antibacterial activity of the juice obtained by squeezing fresh ginger roots (Zingiber officinale) against the standard strains Staphylococcus aureus (ATCC® 25923) and Escherichia coli (ATCC® 25922), as well as Acinetobacter baumannii and Klebsiella pneumoniae isolated from patients hospitalized in intensive care units. Methods: For this purpose, the antibacterial effect of the fresh ginger juice was tested against antibiotics such as ampicillin, cefazolin, cefuroxime, meropenem, colistin, ofloxacin, sulfamethoxazole/trimethoprim, tetracycline, and gentamicin. The antibacterial effects against the tested bacteria were determined by the disc diffusion method using 5 µL, 10 µL, 15 µL and 20 µL amounts of ginger juice impregnated on sterile discs. Results: As a result of the study, it was detected that the antibacterial effect increased in parallel with the increasing amounts of fresh ginger juice in all tested bacteria. Conclusion: Consequently, fresh Zingiber officinale can provide protection to a certain extent against bacterial pathogens due to its antibacterial action.

scholarly journals Synthesis and Antimicrobial Activity of Piperine Analogues Containing 1,2,4-Triazole Ring

2019 ◽  
Vol 31 (5) ◽  
pp. 1077-1080
Author(s):  
Kottakki Naveen Kumar ◽  
Karteek Rao Amperayani ◽  
V. Ravi Sankar Ummdi ◽  
Uma Devi Parimi
Keyword(s):  
Antimicrobial Activity ◽  
Triazole Ring ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Antibacterial Study ◽  
Gram Negative ◽  
E Coli ◽  
Micro Organisms

A series 1,2,4-triazole piperine analogues (TP1-TP6) were designed and synthesized. The structures were confirmed using 1H NMR and 13C NMR. Antibacterial study was done using Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative micro-organisms (E. coli and Pseudomonas aeruginosa) by disc diffusion method. Compound containing chloro substitution (TP6) showed the highest effect, while compound TP1, TP3, TP4, TP5 showed the moderate activity.

scholarly journals Synthesis of a New Conjugates of Imidazole wih Beta Lactam Moiety and Evaluation of its Expanded Antibacterial Activity

2018 ◽  
Vol 34 (5) ◽  
pp. 2495-2501 ◽  
Author(s):  
Sarmd D. Noori ◽  
Mazin N. Mousa ◽  
Shaker A. N. Al-Jadaan
Keyword(s):  
Antibacterial Activity ◽  
Chemical Structure ◽  
Bacterial Species ◽  
Anaerobic Bacteria ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Beta Lactam ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Ft Ir

Five compounds containing (2,4,5-triphenyl triphenyl-1H-) and azetidinone (beta-lactam) moiety were synthesized. The physical data and yield of synthesized compounds were recorded, the chemical structure of prepared compounds were characterized using FT-IR, 1H-NMR and elemental analysis. The antibacterial activity was evaluated using disc diffusion method that involve tow Gram positive (staph. aureus, E. Faecalis), two Gram negative (E. coli and ‎K. pneumoniae), and one anaerobic bacteria (streptococcus. Pyogen). Different concentration of the prepared compounds has been used, and the obtained result were compared with standard (ceftazidime). Compound (5c) showed the best antibacterial activity against all bacterial species while 5a and 5e does not. Other compounds showed activity against some species.

scholarly journals A Study on Antibacterial Activity of Silver Nanoparticles

2018 ◽  
Vol 29 (2) ◽  
pp. 37-41
Author(s):  
M Iqbal Hossain ◽  
M Anwar Habib ◽  
Nazimuddin Ahmed
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Reference Drug ◽  
Gram Negative ◽  
Quasi Experimental

This quasi experimental study was designed to evaluate antibacterial activity of chemically synthesized silver nanoparticles (AgNPs) from silver nitrate (AgNO3) solutions on gram negative bacteria like E.Coli using disc diffusion method. Different concentrations of AgNPs, AgNO3 and reference drug ciprofloxacin were used to find out the antibacterial activity which revealed that AgNPs possessed significant antibacterial effect compared to AgNO3 solutions but relatively less antibacterial effect than that of ciprofloxacin. So chemical synthesis guided AgNPs may have some antibacterial effects.TAJ 2016; 29(2): 37-41

scholarly journals Antibacterial effect of Dimethyl sulfoxide extract of Aloe vera (Aloe barbadensis) leaf gel against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae

Mediscope ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 67-74 ◽  
Author(s):  
Syed Didarul Haque ◽  
Abu Md Mayeenuddin Al Amin ◽  
Baishakhi Islam ◽  
Nazia Nazneen ◽  
Syeda Noorjahan Karim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Dimethyl Sulfoxide ◽  
Antibacterial Effect ◽  
Aloe Vera ◽  
Biologically Active ◽  
Diffusion Method ◽  
E Coli

An exploratory study based on laboratory experiment was carried out to determine the antibacterial effect of Dimethyl sulfoxide (DMSO) extract of Aloe vera leaf gel (DAE) against standard strains of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae in the Department of Pharmacology & Therapeutics in collaboration with the Department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh. DMSO extract was used in five different concentrations (100, 200, 300, 400 and 500 μg/ml). Dose dependent inhibitory effect was seen against the test organisms using disc diffusion method. Zone of inhibition (ZOI) were 8 mm, 13 mm, 15 mm, 16 mm and 21 mm against S. aureus; 0 mm, 8 mm, 13 mm, 15 mm and 18 mm against P. aeruginosa; 8 mm, 11 mm, 13 mm, 16 mm and 20 mm against E. coli; 0 mm, 9 mm, 12 mm, 14 mm and 18 mm against K. pneumoniae at 100, 200, 300, 400 and 500 μg/ml respectively. The minimum inhibitory concentration (MIC) was assessed by broth dilution technique. The MICs of DAE for S. aureus, P. aeruginosa, E. coli and K. pneumoniae were 300 μg/ml, 400 μg/ml, 400 μg/ml and 450 μg/ml respectively. From the study it was observed that DMSO extract of Aloe vera leaf gel possesses antibacterial effect against the test pathogens. The findings highlight the need for further extensive study to detect and isolate the biologically active ingredients present in the Aloe vera leaves which are responsible for antibacterial effect. Hopefully, that would lead to the discovery of new and more potent antimicrobial agents originated from Aloe vera. Mediscope Vol. 7, No. 2: July 2020, Page 67-74

scholarly journals Interpretive criteria to differentiate low- and high-level mupirocin resistance in Staphylococcus aureus

2007 ◽  
Vol 56 (7) ◽  
pp. 937-939 ◽  
Author(s):  
Naira Elane Moreira de Oliveira ◽  
Ana Paula Couto Marques Cardozo ◽  
Elizabeth de Andrade Marques ◽  
Kátia Regina Netto dos Santos ◽  
Marcia Giambiagi deMarval
Keyword(s):  
Staphylococcus Aureus ◽  
High Resistance ◽  
The Other ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Zone Diameter ◽  
Resistant Strains ◽  
Mupirocin Resistance ◽  
High Level

Meticillin-resistant Staphylococcus aureus isolates were classified into three mupirocin susceptibility groups by the disc diffusion method using 5 and 200 μg mupirocin discs. The zone diameter observed for a 5 μg disc distinguished MupS from the resistant strains (either MupRL or MupRH). On the other hand, a 200 μg disc distinguished the high-resistance MupRH strains from the other two (MupS or MupRL). Thus, the concomitant use of 5 and 200 μg mupirocin discs allowed the clear distinction among the three mupirocin susceptibility groups, MupS, MupRL or MupRH.

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