scholarly journals Evaluation of the applicability of polymerase chain reaction (PCR) to sex identification in Eurasian blackbirds (Turdus merula)

2009 ◽  
Vol 46 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Andrzej Dybus ◽  
Anna Siemierz ◽  
Dariusz Wysocki ◽  
Iwona Szatkowska ◽  
Magdalena Muszyńska ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Polymorphism ◽  
Bird Species ◽  
Sex Identification ◽  
Chain Reaction ◽  
Turdus Merula ◽  
Polymerase Chain

Evaluation of the applicability of polymerase chain reaction (PCR) to sex identification in Eurasian blackbirds (Turdus merula)Turdus merulais one of most common bird species in Europe. This study verified a method for its sex identification by PCR with P2/P8 primers, based on theCHD1gene polymorphism, proposed in earlier studies as a universal tool for sex identification in most bird species. Although there are some reports that PCR cannot determine sex in Eurasian blackbirds due to a lack of differences in intron lengths ofCHD1-ZandCHD1-Wgenes, our study showed that such a possibility does exist, so it can contribute to an increased understanding of the biology of this species.

Polymerase Chain Reaction-Based Sex Identification in the Greater Roadrunner

2010 ◽  
Vol 74 (6) ◽  
pp. 1395-1399
Author(s):  
CARLOS A. SANTAMARIA ◽  
SAMUEL KELLEY ◽  
GERRAL G. SCHULZ ◽  
DEAN RANSOM ◽  
LUIS A. HURTADO
Keyword(s):  
Polymerase Chain Reaction ◽  
Sex Identification ◽  
Chain Reaction ◽  
Polymerase Chain

Sex identification of pigeons using polymerase chain reaction analysis with simple DNA extraction

2019 ◽  
Vol 12 (2) ◽  
pp. 45-48
Author(s):  
Shao-jie Liang ◽  
Ming-xia Chen ◽  
Chun-qi Gao ◽  
Hui-chao Yan ◽  
Guo-long Zhang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Polymerase Chain Reaction Analysis ◽  
Sex Identification ◽  
Z Chromosome ◽  
W Chromosome ◽  
Chain Reaction ◽  
Assay Time ◽  
Polymerase Chain ◽  
The Cost

Sex identification plays an important role in avian production. Hitherto, it is difficult to distinguish the sexes of monomorphic birds based on their external features. The chromo-helicase-DNA-binding genes contain CHD-W gene and CHD-Z gene, which are located on the W chromosome and Z chromosome, respectively. Since CHD-W gene is unique to females, the polymerase chain reaction can be used for sex identification. However, extracting DNA procedures for verifying the sex is tedious and expensive. To address these disadvantages, the objective of this study was to develop a simple DNA extraction assay to efficiently process blood, liver, and feather samples. The results showed that 2% dimethylsulfoxide was suitable for processing blood, and phosphate-buffered saline was suitable for processing liver and feather samples. The specific primers were designed, and the length of the targets is 474 bp on Z chromosome and 319 bp on W chromosome. The pigeons were identified as females based on the presence of two bands on the gel, and as males based on the presence of one band. Taken together, our results suggested that feather samples were more appropriate than blood or liver for sex identification of pigeons. Compared to the traditional DNA extraction, this method shortened the assay time and reduced the cost.

Low frequency of CYP2A6 gene polymorphism as revealed by a one-step polymerase chain reaction method

1999 ◽  
Vol 9 (3) ◽  
pp. 327-332 ◽  
Author(s):  
Gen-Fu Chen ◽  
Yong-Ming Tang ◽  
Bridgett Green ◽  
Dong-Xin Lin ◽  
F. Peter Guengerich ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Polymorphism ◽  
Low Frequency ◽  
Polymerase Chain Reaction Method ◽  
Chain Reaction ◽  
Reaction Method ◽  
One Step ◽  
Polymerase Chain ◽  
Cyp2a6 Gene ◽  
Step Polymerase Chain Reaction

Sex determination in ostrich (Struthio camelus) using DNA markers

2010 ◽  
Vol 90 (3) ◽  
pp. 357-360 ◽  
Author(s):  
M. Alipanah ◽  
A. Torkamanzehi ◽  
H. Taghavi
Keyword(s):  
Polymerase Chain Reaction ◽  
Sexual Dimorphism ◽  
Sex Determination ◽  
Sex Chromosome ◽  
Molecular Genetic ◽  
Bird Species ◽  
Rapid Identification ◽  
Chain Reaction ◽  
Struthio Camelus ◽  
Polymerase Chain

Production of bird species such as ostrich (Struthio camelus) has been gaining increasing importance in Iran as well as many other countries. Ostrich, similar to many other species of birds, lacks sexual dimorphism, making it difficult to differentiate between males and females, especially at an early age, which can be problematic in breeding programs. Recently developed molecular genetic methods that utilize polymerase chain reaction (PCR) based techniques can facilitate rapid identification of the bird’s sex in these species using a DNA sample, which can be easily extracted from blood or feather pulps. We successfully applied a PCR-based RFLP technique and sex chromosome primers for sex determination in a sample of 30 Ostrich chicks using DNA extracted from blood and feather pulps. Both DNA samples (blood and feather pulps) provided useful results. However, using feather pulps from 1-day-old chicks can provide an easy and inexpensive method for sex determination in ostrich. Key words: Ostrich (struthio camelus), sex determination, sexual dimorphism, polymerase chain reaction, RFLP

Polymorphisms of LHβ and GnRHR genes and their association with the number of embryos recovered in goats

2014 ◽  
Vol 54 (8) ◽  
pp. 987 ◽  
Author(s):  
M. Z. Fu ◽  
G. Li ◽  
Z. Q. Zhou
Keyword(s):  
Polymerase Chain Reaction ◽  
Single Nucleotide Polymorphisms ◽  
Gene Polymorphism ◽  
Corpus Luteum ◽  
Nucleotide Polymorphisms ◽  
Chain Reaction ◽  
Single Strand Conformational Polymorphism ◽  
Single Nucleotide ◽  
Exon 2 ◽  
Polymerase Chain

The objective of the present study was to explore a predictor of superovulation response on the basis of associations between the number of embryos recovered and gene polymorphism. Variation in the goat LHβ and GnRHR genes was investigated using polymerase chain reaction–single-strand conformational polymorphism and DNA sequencing. Two single nucleotide polymorphisms (SNPs) were identified in the 5′-UTR of LHβ gene (A59C, P1 locus) and in the Exon 2 of GnRHR gene (T177A, P6 locus). At the P1 locus in both breeds, the frequencies of one allele were 0.46 and 0.51, respectively. At the P6 locus, the minor allele frequency was 0.23. Associations of both SNPs with the number of embryos recovered and the corpus luteum number were evaluated in Boer and Shaanbei goat breeds. Association analysis showed that both SNPs had significant (P < 0.05) effects on the number of embryos recovered and corpus luteum number. These results indicate that LHβ and GnRHR genes are potential markers for the number of embryos recovered.

scholarly journals Sex identification by polymerase chain reaction using a Y-autosome homologous primer set

1993 ◽  
Vol 38 (4) ◽  
pp. 429-431
Author(s):  
Taku Iida ◽  
Yutaka Nakahori ◽  
Karo Tanaka ◽  
Osamu Tsutsumi ◽  
Yuji Taketani ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sex Identification ◽  
Chain Reaction ◽  
Polymerase Chain
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