scholarly journals Identifikasi Aksi Gen Epistasis pada Toleransi Kedelai terhadap Cekaman Aluminium

2015 ◽  
Vol 43 (1) ◽  
pp. 30 ◽  
Author(s):  
Arvita Netti Sihaloho ◽  
Trikoesoemaningtyas . ◽  
Didy Sopandie ◽  
Desta Wirnas

The efectiveness of selection depends on the gene action cotrolling the character.  The presence of epistatic gene action will delay the genetic gain in self pollinated crops like soybean. The objective of this research was to obtain information on genetic control of soybean tolerance to aluminum toxicity on nutrient culture. This research was conducted at green house of  the University Farm IPB Cikabayan, Bogor, from April to June 2013. This research used 120 seedlings of F2 population  generated from  crossing between Argomulyo (Al-sensitive) with Tanggamus (Al-tolerant). Seedlings were grown in nutrient culture for 14 days with Al concentration of 1.5 mM and pH 4.0, to observe the vegetative growth under aluminum stress condition. The result showed that mean of roots length, shoot length, shoot to root ratio, wet and dry weight of root and shoot exceeded  both parents mean value with moderate to high heritability. All observed characters had continue distribution pattern, and controlled by many genes. Duplicate epistasis additive gene action controlled only root length while other characters were controlled by complementary epistasis gene action.<br />Keywords: gene action, heritability, nutrient culture, selection, tocixity

2019 ◽  
Vol 47 (2) ◽  
pp. 163-170 ◽  
Author(s):  
Marina Yuniawati Maryono ◽  
Trikoesoemaningtyas , ◽  
Desta Wirnas ◽  
Dan Soeranto Human

The genetic analysis and selection of transgressive segregants on agronomic characters are required in a sorghumbreeding program for obtaining high proportion homozygosity rapidly in the early generations. This study aimed to obtainthe information on the performance of agronomic characters, estimate genes’ action, broad-sense heritability, coefficient ofgenetic variability, and select transgressive segregant on F2 population of sorghum based on grain yield. The research wasconducted from July 2014 until October 2014 in Dramaga, Bogor. About 300 and 150 individuals F2 populations of B69 ×Numbu and B69 × Kawali, respectively, were used as genetic materials. The results showed that plant height and paniclelength of B69 × Numbu and B69 × Kawali populations were controlled by many genes with additive gene action. The grainyield and grain yield per panicle were controlled by many genes with additive gene action and complementary epistasis,had high heritability, and large coefficients of genetic variability. The transgressive segregant selection had selected 14individuals of B69 × Numbu and 134 individuals of B69 × Kawali populations based on grain yield per panicle.


2015 ◽  
Vol 3 (1) ◽  
pp. 73-79
Author(s):  
Golam Sarwar ◽  
Md. Sarowar Hossain ◽  
Md.Harun -Ur- Rashid ◽  
Shahanaz Parveen

The present study was conducted in the experimental farm, Sher-e-Bangla Agricultural University (SAU), Dhaka during July 2013-December2013. The analysis of variance revealed significant deviation for all the characters studied and indicated the existence of variation among thegenotypes. The PCV values were slightly higher than the respective GCV values for all the characters except unfilled grains per panicleindicating that the characters were less influenced by the environment. Total tillers per plant, effective tillers per plant, filled grains per panicle,unfilled grains per panicle and yield per plant showed high heritability coupled with high genetic advance percentage of mean which indicatedthe preponderance of additive gene action and such characters could be improved through selection. High heritability along with low geneticadvance as percentage of mean was found for plant height, days to 50% flowering, panicle length, days to maturity and thousand grains weightwhich indicated the non additive gene action for expression of these characters. Considering the genetic parameters and other agronomicperformances, the genotypes Special from AL-29, AL-36, PP-4B(i), AL-17(iii)B, AL-17(iii), AL-17(ii)A, Special from-129, Special from17(iv), AL-44(i), AL-17, Special from AL-36(D), PP-48, IR-25B, Special from AL-33, IR-25B (Tall), P-5B (ii) might be considered betterparents for future hybridization programme.DOI: http://dx.doi.org/10.3126/ijasbt.v3i1.11896    Int J Appl Sci Biotechnol, Vol. 3(1): 73-79 


2017 ◽  
Vol 9 (3) ◽  
pp. 1854-1860 ◽  
Author(s):  
Karmvir S. Garcha ◽  
A. S. Dhatt ◽  
M. K. Sidhu

Twelve genotypes of eggplant in BC3, BC4 and BC5 generations of CMS-lines derived from Solanum ae-thiopicum × Solanum melongena cross along with their maintainers were evaluated for eighteen quantitative charac-ters at Punjab Agricultural University Ludhiana. Analysis of variance depicted significant variation (P ≤ 0.05) for all the characters in all generations. High PCV and GCV values were observed for fruit length, fruit girth, fruit weight, number of fruits plant-1 and yield plant-1 in all generations indicating high variability in the germplasm. High heritability coupled with high genetic advance was found for peduncle length, fruit length, fruit girth, fruit weight, number of fruits plant-1 and yield plant-1 in all generations indicating the predominance of additive gene action for these traits. Thus, selections can be made from present germplasm for the development of improved CMS inbred lines with varying fruit traits.


2017 ◽  
Vol 45 (2) ◽  
pp. 162 ◽  
Author(s):  
Siti Nurhidayah ◽  
Yudiwanti Wahyu ◽  
Dan Willy Bayuardi Suwarno

<p align="center"><strong>ABSTRACT</strong></p>Selection of transgressive segregants is considered as one effective way of obtaining peanut genotypes with high yield on early segregation. The objective of the study was to estimate genetic parameters and to identify transgressive segregants of peanut family in F3 generation. The experiment was conducted at Leuwikopo, Dramaga, Bogor on August-Desember 2014. The genetic materials evaluated were 218 family F3 generation from 5 biparental population (Jerapah/GWS79A1, GWS79A1/Zebra, GWS79A1/Jerapah, Zebra/GWS79A1 and Zebra/GWS18) and 4 commercial varieties as checks (Gajah, Jerapah, Sima, and Zebra). The experiment was conducted in an augmented randomized complete block design with 4 replications for the checks. The results showed that quantitative characters had continuous distribution and they were controlled by many genes with additive, dominance, complementary epistasis, or additive epistasis gene action. Total number of pod and number of pod had high heritability and moderate genetic coefficient of variation. Selection with 10% intensity showed that 22 families had large total number of pods while six families had larger total number of pods than both parents. Family U2-39 estimated was identified as a putative transgressive segregant with high total number of pod and had smaller variance within family than Sima variety. <br /><br />Keywords: additive,gene action, heritability, GCV


Author(s):  
Akashi , , , , , Sarma ◽  
V. J. Dhole ◽  
A. Bhattacharjee ◽  
P. Das ◽  
D. Sarma ◽  
...  

Uniform and healthy seeds of green gram cv. Pratap (SG-1) were treated with two mutagens, gamma rays (physical mutagen) and EMS (chemical mutagen) alone and in combination. The two hundred forty M1 progenies were laid in randomized block design with three replications during Kharif, 2017 to raise M2 generation. Analysis of variance discloses significant differences among the treatments for all the eight characters studied. It was observed that in general, the combination dose, i.e., 200 Gy+0.2% EMS gave superior results in almost all the yield attributing characteristics. High heritability coupled with high genetic advance was recorded for seed yield per plant, which indicates the predominance of additive gene action. Number of pods per plant followed by pod length showed high significant positive correlation with seed yield per plant. The character number of pods per cluster had shown positive correlation with seed yield per plant, but path analysis revealed its negative direct effect on seed yield.


2019 ◽  
Vol 17 (3) ◽  
pp. 295-300
Author(s):  
Taslima Aktar ◽  
Md Nuruzzaman ◽  
Md Shoheh Rana ◽  
Md Monjurul Huda ◽  
Md Amir Hossain ◽  
...  

The potential of a crop to favorably respond to breeding programs depends on its existing nature and magnitude of genetic variability, heritability and genetic advance. An experiment was conducted with eighteen Brassica genotypes following Randomized complete block design (RCBD) with three replications at the field of the department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh, Bangladesh to estimate genetic divergence, variability ranges and contribution of each trait towards genetic diversity. Analysis of variance showed significant variation among the genotypes for all the studied yield and yield contributing characters. The phenotypic co-efficient of variation (PCV) was higher than the genotypic co-efficient of variation (GCV) for all the characters measured, indicated that the traits were influenced by environment. All traits showed high heritability (61.54% to 98.64%) which indicated possible improvement through selection. High heritability values (>60%) along with high genetic advance and genetic advance in percentage of mean indicated prevalence of additive gene action, which provides good scope for further improvement by selection. The genotypes were grouped into four clusters, most of the genotypes were grouped into Custer I (8 genotypes), while cluster IV contained only one genotype. Considering the mean value, the cluster IV and Cluster II were ranked as the top groups in terms of yield and early maturity. High Heritability with high genetic advance in percentage of mean was observed for number of branches/plant, number of pod/plant, number of seeds/pod and yield/plant and selection focused   on these traits could be useful for future breeding program of Brassica. The genotype BD-7114 was the best performer considering all the studied traits especially yield and days to maturity which could be used as an important breeding material for the improvement of Brassica. J Bangladesh Agril Univ 17(3): 295–300, 2019


Author(s):  
Abenezer Abebe ◽  
Zelalem Tafa ◽  
Worknesh Terefe

Maize is one of the most important cereals broadly adapted worldwide. Though, a number of improved maize varieties have been released, each micro-environment has not been touched that is why the study carried out. The experiment was conducted using seven maize varieties in RCB design with three replications. The analysis of variance signifies the presence of significant difference (p<0.05) among the seven maize varieties evaluated. High value of genetic (GCV) and phenotypic coefficient of variation (PCV) for grain yield (25.1 and 37.8%) were estimated and this infers less influence of environment. Additionally, moderately high heritability (44.2%) and high genetic advance in percent mean (34.4%) were estimated for grain yield which indicate the trait governed by additive gene action and could be improved via selection based on phenotypic performance. However, traits (male and female flower) with high heritability and moderate genetic advance in percent mean inherited mostly by non-additive gene action and heterosis breeding could be useful. Regarding agronomic performance, Hora maize variety provided highest grain yield (5.0 t/ha) followed by Kuleni (4.1 t/ha), Melkasa 2 (4.0 t/ha) and check (4.0 t/ha). Hora, Melkasa 2, Melkasa 4 and check flowered earlier as compared to the other and could be used as parent for generating early flowering varieties. In summary, Hora maize variety was better performing both statistically and in eyes of farmers and need seed multiplication and distribution to farming community. Moreover, the variability observed among the maize varieties could be utilizing in future breeding activities.


2011 ◽  
Vol 8 (1) ◽  
pp. 31-38
Author(s):  
Dwi Hapsoro ◽  
Hajrial Aswidinnoor ◽  
Rusmilah Suseno ◽  
Jumanto Jumanto ◽  
Sudarsono Sudarsono

Inheritance of Resistance to PStV in Transgenic Peanuts Containing cp PStV Gene. We have obtained transgenic peanut lines containing coat protein gene of PStV. To get maximal use of the transgenic character in a breeding program, it is necessary that the transgene is also stably inherited and expressed.  This experiment was conducted from June 2002 – January 2005 at Plant Molecular Biology Laboratory, Bogor Agricultural University, and Queensland Agricultural Biotechnology Center, The University of Queensland, Australia. The research aimed (1) to test whether PStV cp transgene was functional in progenies derived from crosses between transgenic peaanut plants containing PStV cp gene and non-transgenic ones and (2) to determine pattern of inheritance of resistance to PStV as a result of PStV  cp gene action. Several crosses were made between trangenic peanut cv.Gajah resistant to PStV (T4 generation) and non-transgenic peanut line WS susceptible to PStV. The F1 and F2 populations were mechanically inoculated with PStV two weeks after planting. The experiment showed that all plants in the F1 population were less susceptible to PStV, suggesting that the transgene was partially dominant.  Phenotipic segregation in F2 population was not Mendelian with the appearance of quick and slow recovery plants and the number of resistant plants being more than expected. However, the proportion of transgenic and non-transgenic plants followed 3:1 ratio, which was Mendelian.


2017 ◽  
Vol 45 (1) ◽  
pp. 43-48
Author(s):  
Tri Lestari ◽  
Trikoesoemaningtyas , ◽  
Sintho Wahyuning Ardie ◽  
Dan Didy Sopandie

The management of P nutrition can be a good solution for aluminum toxicity and P nutrient deficiency in acid soil. This study aimed to determine the role of phosphorus in improving the tolerance of sorghum to Al stress in nutrient culture. This research was conducted at green house of IPB Bogor, tissue culture laboratory in the Department of AGH IPB and Balai Besar Pasca Panen Cimanggu Bogor, from January to November 2014. A completely randomized factorial design was used in three experiments. The results revealed that addition of P improved the tolerance of two sorghum genotypes to Al stress as indicated by the reduction in root length inhibition by Al, where  P was more effective in tolerant genotype Numbu. Addition of P reduced the accumulation of Al in the root tissues as shown by the lighter intensity of hematoxylin staining, especially in Numbu. Aluminum stress increased the secretion of oxalate acid in both sorghum genotypes, where P lowered oxalate acid secretion in both genotypes. These facts showed that the role of P in improving the tolerance of sorghum to Al stress might be associated with the inhibition of Al absorption into the root tissues, although the mechanism is not yet known.Keywords: Al stress, organic acid secretion, role of P, root staining method, sorghum


2021 ◽  
Vol 23 (3) ◽  
pp. 346-350
Author(s):  
J. JOHNNY SUBAKAR IVIN ◽  

In the present investigation, the four cross combinations of Punjab Padmini X Panruti local, Dharmapuri local X Panruti local, Punjab Padmini X Parbanikranthi and Dharmapuri local X Parbanikranthi and three bhendi populations, BIP, F2, and F3, were developed in 2019, and these populations were evaluated in 2020 to determine the extent of genetic variability, heritability, and genetic advance for twelve bhendicharacters. Significant variances in BIP compared to the population of F2 and F3 in most characters have been noted that showed high mean and wider variations in PCV and GCV in fruit length, number of fruits per plant and plant yield. High heritability of BIPS was revealed by Punjab Padmini x Parbanikranthi for the traits viz., days to 50 per cent flowering, number of fruits per plant, fruit length, fruit girth and fruit yield per plant which revealed the importance of additive gene action for these traits. This is indicative of both additive and non-additive gene action (dominance and epistasis). Hence, selection could not be practiced in the immediate segregating generations. These two characters also showed unfavourable association with fruit yield per plant in BIPs which indicated that all the unfavourable linkages were not broken down through intermating in the early segregating generation. Thus, selection should be postponed to later generations after intermating in one or two subsequent to obtain potential recombinants.


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