scholarly journals Zoonotik Bir Patojen Olan Salmonella’nın Bitki Konaklarındaki Davranışı

Author(s):  
Elim Gamze Has ◽  
Mustafa Akçelik

Salmonella is known as one of the main factors of food-borne gastroenteritis, and with this feature, it poses a great risk in terms of public health and economic losses. It is estimated that of the approximately 94 million cases of salmonellosis occurring in the world each year (about 85 percent of these are food-borne), an average of 150,000 result in death. Current social trends highlight the important health benefits of fresh produce in our daily diet. As an irony; Uncooked consumption of fresh herbal products is increasingly identified as a source of transmission for pathogens of intestinal origin, and epidemics occurring in this way are spreading rapidly. Today, the frequency of anthropogenic pathogen outbreaks associated with fresh produce, spices, and nuts has surpassed those associated with foods of animal origin. Human pathogens in the production chain; It can be transferred to plant material by basic means such as the use of animal manure, contaminated irrigation water, biological vectors (insects and animals) and contaminated seeds. In the light of these data, many intestinal pathogens have been identified as phyllo sphere-associated bacteria. Despite all these findings, there are many unknowns on the persistence and infectivity of Salmonella in contaminated plant hosts. In this review article, the factors affecting the attachment, colonization and survival of Salmonella on plant surfaces, as well as the information on the infection processes that continue with the invasion of plant tissues are discussed in the light of contemporary literature data.

2019 ◽  
Vol 366 (23) ◽  
Author(s):  
Jasper Schierstaedt ◽  
Rita Grosch ◽  
Adam Schikora

ABSTRACT Food-borne diseases are a threat to human health and can cause severe economic losses. Nowadays, in a growing and increasingly interconnected world, food-borne diseases need to be dealt with in a global manner. In order to tackle this issue, it is essential to consider all possible entry routes of human pathogens into the production chain. Besides the post-harvest handling of the fresh produce itself, also the prevention of contamination in livestock and agricultural soils are of particular importance. While the monitoring of human pathogens and intervening measures are relatively easy to apply in livestock and post-harvest, the investigation of the prevention strategies in crop fields is a challenging task. Furthermore, crop fields are interconnected with livestock via fertilizers and feed; therefore, a poor hygiene management can cause cross-contamination. In this review, we highlight the possible contamination of crop plants by bacterial human pathogens via the rhizosphere, their interaction with the plant and possible intervention strategies. Furthermore, we discuss critical issues and questions that are still open.


2007 ◽  
Vol 30 (4) ◽  
pp. 93
Author(s):  
I Sekirov ◽  
N Tam ◽  
M Robertson ◽  
C Lupp ◽  
B Finlay

Background: During our lifetimes we develop a very complex set of interactions with the multitude of microorganisms colonizing our bodies. In the gastrointestinal system, the microbiota is highly important for morphological development, nutrition, and protection against infectious diseases. The gastrointestinal pathogens, enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) and Salmonella enterica serovar Typhimurium (ST) are food-borne pathogens that cause much morbidity and mortality worldwide. Citrobacter rodentium (Cr) is a mouse pathogen that is used in small animal models to mimic EHEC and EPEC infections. Methods: We began to characterize the contribution of intestinal microbiota to the progression of these infections. Two main phyla comprise the majority of mouse intestinal microbiota: Bacteroidetes and Firmicutes. Bacteria from a number of additional phyla are also present in smaller numbers; among them γ-Proteobacteria class, belonging to Proteobacteria phylum, is note-worthy as this class harbours many intestinal pathogens, such as ST and Cr. The mouse intestinal microbiota was perturbed using tetracycline (Tet) and streptomycin (Sm) to increase the proportion of Bacteroidetes in the colonic microbiota, and using vancomycin (Vanc) to create a predominance of Firmicutes. The mice with this perturbed microbiota were infected with ST to investigate the resultant pathology and virulence characteristics, and any additional shifts in microbiota as a result of infection. Results: Treatment of mice with Sm and Vanc was found to decrease the resistance of mice to colonization with ST, while Tet-treated mice exhibited unchanged colonization resistance. Treatment of mice with gradually increasing doses of Sm, which gradually augmented the proportion of CFB bacteria in the microbiota, resulted in progressively increasing colonization of mice by ST, as well as a step-wise increase in the ST-induced typhlitis, associated with higher levels of inflammatory markers IL-6 and KC. The increasing levels of ST colonization following both Sm and Vanc treatment were associated with an increase in the proportion of γ-Proteobacteria in the cecal and colonic microbiota, as well as a decrease in the total bacterial numbers in both organs. Conclusions: It is evident that the intestinal microbiota plays a significant role in the host’s response to infection with enteric pathogens, and its composition and numbers are also affected by the offending bacteria. Elucidation of the details regarding the contribution of the microbiota to infectious disease progression will offer novel targets for the future design of superior prevention and treatment methods.


AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Quan Li ◽  
Jian Yin ◽  
Zheng Li ◽  
Zewei Li ◽  
Yuanzhao Du ◽  
...  

AbstractSalmonella is an important food-borne pathogen associated with public health and high economic losses. To investigate the prevalence and the characteristics of Salmonella in a pig slaughterhouse in Yangzhou, a total of 80 Salmonella isolates were isolated from 459 (17.43%) samples in 2016–2017. S. Derby (35/80, 43.75%) was the most prevalent, followed by S. Rissen (16/80, 20.00%) and S. Newlands (11/80, 13.75%). The highest rates of susceptibility were observed to cefoxitin (80/80, 100.0%) and amikacin (80/80, 100.0%), followed by aztreonam (79/80, 98.75%) and nitrofurantoin (79/80, 98.75%). The highest resistance rate was detected for tetracycline (65/80, 81.25%), followed by ampicillin (60/80, 75.00%), bactrim (55/80, 68.75%), and sulfisoxazole (54/80, 67.50%). Overall, 91.25% (73/80) of the isolates were resistant to at least one antibiotic, while 71.25% (57/80) of the isolate strains were multidrug resistant in the antimicrobial susceptibility tested. In addition, 86.36% (19/22) of the 22 antimicrobial resistance genes in the isolates were identified. Our data indicated that the resistance to certain antimicrobials was significantly associated, in part, with antimicrobial resistance genes. Furthermore, 81.25% (65/80) isolates harbored the virulence gene of mogA, of which 2 Salmonella Typhimurium isolates carried the mogA, spvB and spvC virulence genes at the same time. The results showed that swine products in the slaughterhouse were contaminated with multidrug resistant Salmonella commonly, especially some isolates carry the spv virulence genes. The virulence genes might facilitate the dissemination of the resistance genes to consumers along the production chain, suggesting the importance of controlling Salmonella during slaughter for public health.


3 Biotech ◽  
2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Chetan Sharma ◽  
Brij Pal Singh ◽  
Nishchal Thakur ◽  
Sachin Gulati ◽  
Sanjolly Gupta ◽  
...  

Author(s):  
Kaknokrat Chonsin ◽  
Ruchirada Changkwanyeun ◽  
Achiraya Siriphap ◽  
Apiradee Intarapuk ◽  
Watsawan Prapasawat ◽  
...  

Salmonella causes foodborne disease outbreaks worldwide and raises considerable concerns about public health and economic losses. To determine prevalence, serovar, antimicrobial resistance (AMR) patterns, and extended-spectrum beta-lactamase (ESBL) genes, the present cross-sectional study collected a total of 418 fecal, carcass (three slaughterhouses), pork and cutting board (four markets) samples from a province in central Thailand in 2017 and 2018. Results showed that 65.1% (272/418) of samples were positive for Salmonella. The percentage of Salmonella positive samples from markets (88.8%; 158/178) was significantly higher than those from slaughterhouses (47.5%; 114/240) ( P<0.05 ). In total, 1,030 isolates were identified; of these, 409 were assigned to 45 serovars with S. Rissen (20%; 82/409) being the most common. New serovars of Thai isolates, S. Cannstatt and S. Braubach, were identified in market and slaughterhouse samples, respectively. AMR of Salmonella isolates showed that 73.9% (133/180) of 19 different serovars exhibited multidrug resistance (MDR). Screening for ESBL production showed that 10.3% (41/399) of isolates were ESBL positive. ESBL-producing Salmonella isolates in market samples (75.6%; 31/41) were significantly higher than those in slaughterhouse samples (24.4%; 10/41) ( P<0.05 ). In market samples, 77.4% (24/31) were isolated from pork and 22.6% (7/31) from cutting boards. Nine ESBL-producing isolates carried single type ESBL genes bla TEM (9.8%; 4/41) or bla CTX-M (12.2%; 5/41), while 11 (26.8%) carried both bla TEM and bla CTX-M . No ESBL-producing Salmonella isolate carried the gene bla SHV . Results suggest that pigs, their flesh, and cutting boards could be reservoirs for widespread MDR, ESBL-producing Salmonella outbreak across the food chain.


Author(s):  
Marie Limoges ◽  
Deborah A. Neher ◽  
Thomas R. Weicht ◽  
Patricia D. Millner ◽  
Manan Sharma ◽  
...  

Composted or heat-treated Biological Soil Amendments of Animal Origin (BSAAO) can be added to soils to provide nutrients for fresh produce. These products lower the risk of pathogen contamination of fresh produce when compared with use of untreated BSAAO; however, meteorological conditions, geographic location, and soil properties can influence the presence of pathogenic bacteria, or their indicators (e.g., generic E. coli) and allow potential for produce contamination. Replicated field plots of loamy or sandy soils were tilled and amended with dairy manure compost (DMC), poultry litter compost (PLC), or no compost (NoC) over two different field seasons, and non-composted heat-treated poultry pellets (HTPP) during the second field season. Plots were inoculated with a three-strain cocktail of rifampicin-resistant E. coli (rE.coli) at levels of 8.7 log CFU/m2. Direct plating and most probable number (MPN) methods measured the persistence of rE.coli and Listeria spp. in plots through 104 days post-inoculation. Greater survival of rE. coli was observed in PLC plots in comparison to DMC plots and NoC plots during year 1 (P < 0.05). Similar trends were observed for year 2, where rE. coli survival was also greater in HTPP amended plots (P < 0.05). Survival of rE. coli was dependent on soil type, where water potential and temperature were significant covariables. Listeria spp. were found in NoC plots, but not in plots amended with HTPP, PLC or DMC. Radish data demonstrate that PLC treatment promoted the greatest level of rE.coli translocation when compared to DMC and NoC treatments (P  < 0.05). These results are consistent with findings from studies conducted in other regions of the US and informs Northeast produce growers that composted and non-composted poultry-based BSAAO supports greater survival of rE. coli in field soils. This result has the potential to impact the food safety risk of edible produce grown in BSAAO amended soils as a result of pathogen contamination.


2022 ◽  
pp. 399-434
Author(s):  
P. Bosi ◽  
◽  
D. Luise ◽  
P. Trevisi ◽  
◽  
...  

Intestinal pathogens causing either clinical or sub-clinical infections increase pig morbidity and (or) mortality, resulting in economic losses and wider socio-economic impacts on pig production. An optimally functioning gastrointestinal tract (GIT) is fundamental to combatting intestinal pathogen colonisation at all the stages of life. This requires successful development and maintenance of key gut functions: digestive function; the gastro-intestinal cell line barrier; gut-associated lymphoid tissue (GALT); and gut-associated microbiota. This chapter first discusses research on genes associated with pathogen resistance and porcine immune response. It then reviews risk factors associated with gut mucosa impairment as well as dietary strategies to control risk factors and improve gut functionality in preventing intestinal pathogen colonisation.


2018 ◽  
Vol 38 (3) ◽  
pp. 393-399 ◽  
Author(s):  
Débora D.S. Gobbi ◽  
Maria G. Spindola ◽  
Luisa Z. Moreno ◽  
Carlos E.C. Matajira ◽  
Maria G.X. Oliveira ◽  
...  

ABSTRACT: Arcobacter is an emerging zoonotic pathogen, and the major transmission routes to humans are the handling or consumption of contaminated raw/undercooked food products of animal origin, water and seafood. The isolation and identification of Arcobacter species are not routine in clinical laboratories; therefore, its true incidence in human infections may be underestimated. The present study aimed to isolate and characterize Arcobacter from carcasses and fecal samples collected at swine slaughterhouses and from meat markets in São Paulo State, Brazil. The isolates were identified using multiplex-PCR to differentiate the species and analyzed by single-enzyme amplified fragment length polymorphism (SE-AFLP). Arcobacter spp. were isolated from 73.0% of swine carcasses, 4% of fecal samples and 10% of pork samples. A. butzleri was the most prevalent species identified, followed by A. cryaerophilus. Interestingly, the carcasses presented higher frequency of A. butzleri isolation, whereas only A. cryaerophilus was isolated from fecal samples. SE-AFLP enabled the characterization of A. butzleri and A. cryaerophilus into 51 and 63 profiles, respectively. The great genetic heterogeneity observed for both species corroborates previous reports. This study confirms the necessity for a standard isolation protocol and the improvement of molecular tools to further elucidate Arcobacter epidemiology.


Nematology ◽  
2015 ◽  
Vol 17 (4) ◽  
pp. 447-457 ◽  
Author(s):  
Yulia Kroupitski ◽  
Riky Pinto ◽  
Patricia Bucki ◽  
Edward Belausov ◽  
Liliane Ruess ◽  
...  

The objective of the present study was to assess ifAcrobeloides buetschlii, an opportunistic species common across many soils, can be employed as a suitable model for interactions between free-living soil nematodes and enteric human pathogens.Acrobeloides buetschliiwas exposed to mCherry-taggedSalmonella entericaandEscherichia coliO157:H7 and its vector potential was assessed.Salmonellacells were more readily ingested by the nematodes compared toE. coliO157:H7. Adult nematodes ingested more bacteria compared to juveniles.Salmonellasurvived internally for at least 7 days without affecting the viability of nematodes. Bacterial ingestion byA. buetschliidid not vary for three testedSalmonellaserovars but was significantly lower forE. coliO157:H7. Considering the ubiquitous nature of pathogen and vector, these findings suggest thatA. buetschliican serve as a relevant model for studying nematode-Salmonellainteractions in an agricultural setting and as potential transport for food-borne pathogens from soil to crops.


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