scholarly journals Phylogenetic Analysis of Striped Hyaena (Hyaena hyaena: Hyaenidae, Carnivora) in Hatay Province in Turkey

Author(s):  
Erol Atay ◽  
Mustafa Ersal ◽  
Kemal Karabağ ◽  
İsmail Turan Çetin

Striped hyaena (Hyaena hyaena) is one of the species in danger of extinction and categorized globally as “Under Threatened Organism”. From time to time, different tissue samples and carcasses of the striped hyena are reported in different regions of Anatolia. In this study, 571 bp length of Cytochrome C Oxidase Subunit II (COX 2) of mitochondiral DNA from hair, ears, nails and teeth specimens from six striped hyaenas were amplified and sequenced to determined phylogenetic relationships between close and distant species related to hyaena. Tissue samples using in this study were found randomly at different times in Hatay province, Turkey. According to our results, all colected samples located in Hatay region are the members of H. hyaena species. Moreover, this research is the first molecular research using COX2 gene region for phylogenetic analysis in Turkey. Further investigation can be performed on studies that suggest determining phylogenetic status of striped hyaenas.

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 299
Author(s):  
Georgios Sioutas ◽  
Styliani Minoudi ◽  
Katerina Tiligada ◽  
Caterina Chliva ◽  
Alexandros Triantafyllidis ◽  
...  

Dermanyssus gallinae (the poultry red mite, PRM) is an important ectoparasite in the laying hen industry. PRM can also infest humans, causing gamasoidosis, which is manifested as skin lesions characterized by rash and itching. Recently, there has been an increase in the reported number of human infestation cases with D. gallinae, mostly associated with the proliferation of pigeons in cities where they build their nests. The human form of the disease has not been linked to swallows (Hirundinidae) before. In this report, we describe an incident of human gamasoidosis linked to a nest of swallows built on the window ledge of an apartment in the island of Kefalonia, Greece. Mites were identified as D. gallinae using morphological keys and amplifying the Cytochrome C oxidase subunit I (COI) gene by PCR. Bayesian phylogenetic analysis and median-joining network supported the identification of three PRM haplogroups and the haplotype isolated from swallows was identical to three PRM sequences isolated from hens in Portugal. The patient was treated with topical corticosteroids, while the house was sprayed with deltamethrin. After one week, the mites disappeared and clinical symptoms subsided. The current study is the first report of human gamasoidosis from PRM found in swallows’ nest.


2021 ◽  
Vol Vol 66 (1) (January (1)) ◽  
pp. 1-5
Author(s):  
Jerome Goddard ◽  
Gerald Baker ◽  
Petra Jericke ◽  
Lawrence Birchman ◽  
Ethan Woodward ◽  
...  

Ultrastructural and molecular data are provided from a single adult female pentastomid opportunistically collected from a road-killed rattlesnake in Russell, KS. Ultrastructural data consisted of light and SEM microscopy of the pentastomid and its eggs, while molecular data consisted of partial 18S and 28S ribosomal sequences and a partial cytochrome c oxidase subunit 1 sequence from the same specimen used for SEM. Ultrastructural and molecular data support generic identification of the pentastomid as Porocephalus sp. These molecular data were also used with previously published pentastomid sequence data for a concatenated phylogenetic analysis, which support the current, morphology-based taxonomic placement of the genus.


2017 ◽  
pp. 155-176
Author(s):  
Miguel Lozano-Terol ◽  
María Juliana Rodríguez-García ◽  
José Galián

En este estudio se analizan dos fragmentos del gen de la citocromo c oxidasa subunidad I (COX1) del ADN mitocondrial de 61 individuos del género Rhynchophorus colectados en la Región de Murcia a fin de determinar su procedencia. El análisis filogenético del fragmento 1 de las muestras de la Región de Murcia conjuntamente con las secuencias disponibles en GenBank indica que los individuos corresponden a la especie Rhynchophorus ferrugineus.Las secuencias de Murcia se colapsan en un único haplotipo (H8 mediterráneo) que aparece dentro del clado de R. ferrugineus. De los análisis filogeográficos se infiere que el origen de los individuos de Murcia es Egipto. Adicionalmente, se examinó una región contigua del COX1 (fragmento 2) en la que las secuencias se colapsaron en dos haplotipos. In this research two fragments of the cytochrome c oxidase subunit I (COX1) gene of the mitochondrial DNA were analyzed in 61 individuals of the genus Rhynchophorus collected in the Region of Murcia with the aim of determining their origin. Phylogenetic analysis of fragment 1 of the samples collected in the Region of Murcia together with the available sequences in GenBank, indicated that these individuals correspond to the species R. ferrugineus. Sequences from Murcia collapsed into the H8 Mediterranean haplotype, which cluster into the R. ferrugineus clade. Phylogeographic analysis shows that the origin of the individuals collected in the Region of Murcia is Egypt. Additionally, a contiguous fragment of COX1 (fragment 2) was analyzed and the sequences collapsed into two haplotypes.


Zootaxa ◽  
2008 ◽  
Vol 1691 (1) ◽  
pp. 67 ◽  
Author(s):  
M. ALEX SMITH

The 5' end (Folmer or Barcode region) of cytochrome c oxidase 1 (CO1) has been proposed as the gene region of choice for a standardized animal DNA barcode (Hebert et al. 2003). Concerns have been raised regarding the decision to utilize this particular mitochondrial gene region as a barcode. Nevertheless, widely divergent taxonomic groups have reported success using CO1 for both species identification and discovery. The utility of CO1 for barcoding amphibians was raised early on (Vences, et al. 2005) and concerns for this group were reported widely (Waugh 2007)—although some considered that the reporting of the concerns outstripped the data that had been analyzed at that point (Smith et al. 2008). Indeed, our analysis of CO1 for a small group of Holarctic amphibians was neither more difficult to generate nor to analyze than for other groups where we have utilized the technique.


2009 ◽  
Vol 23 (3) ◽  
pp. 223 ◽  
Author(s):  
Y. W. Sin ◽  
Joelle C. Y. Lai ◽  
Peter K. L. Ng ◽  
K. H. Chu

The phylogenetic relationships between 10 of 13 genera of crabs from the superfamily Dorippoidea were investigated using mitochondrial 16S rRNA, 12S rRNA and cytochrome c oxidase subunit I gene sequences. The resultant phylogenetic trees based on the three molecular markers support the division of Dorippidae and Ethusidae as monophyletic families within the Dorippoidea. The inferred inter-generic relationships within Dorippidae concur with groupings based on the overall morphology of the carapace and structures of the male first pleopods.


Zootaxa ◽  
2017 ◽  
Vol 4247 (3) ◽  
pp. 201 ◽  
Author(s):  
JIA HUANG ◽  
YIRUI SU ◽  
HONGWEI CHEN

Seventeen species of Leucophenga subpollinosa species group are described from China (including 11 new species): L. argentina (de Meijere, 1924); L. flavicosta Duda, 1926; L. formosa Okada, 1987; L. nigroscutellata Duda, 1924; L. subpollinosa (de Meijere, 1914); L. umbratula Duda, 1924; L. aculeata sp. nov.; L. acuticauda sp. nov.; L. cultella sp. nov.; L. cyclophylla sp. nov.; L. flaviclypeata sp. nov.; L. fuscipedes sp. nov.; L. gracilenta sp. nov.; L. latipenis sp. nov.; L. magnicauda sp. nov.; L. rhombura sp. nov.; L. rugatifolia sp. nov. A key to all examined species of the subpollinosa group in the present study is provided. The phylogenetic relationships among the 17 species of the subpollinosa group are reconstructed by NJ (Neighbor-joining) and Bayesian analyses using 98 DNA sequences of COI (cytochrome c oxidase subunit I) gene. The pairwise intra- and interspecific p-distances of aforementioned sequences are summarized. 


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