scholarly journals Antioksidan Ekstrak Daun Sumpit (Brucea javanica (L). Merr) dengan Metode DPPH

2019 ◽  
Vol 2 (1) ◽  
pp. 57-62
Nurul Fitriani ◽  
Herman Herman ◽  
Laode Rijai

Telah dilakukan penelitian yang berjudul Aktivitas Antioksidan Ekstrak Daun Sumpit (Brucea javanica (L.) Merr.) dengan metode DPPH. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan, nilai IC50, ekstrak yang memiliki aktivitas terbaik sebagai antioksidan, serta mengetahui potensi antioksidan daun sumpit (Brucea javanica (L.) Merr.) dibandingkan dengan kontrol positif vitamin C. Penelitian ini menggunakan metode peredaman radikal bebas DPPH (1,1-diphenyl-2-picrylhydrazyl) yang diujikan pada ekstrak kasar metanol, ekstrak fraksi n-heksana, ekstrak fraksi etil asetat, dan ekstrak fraksi n-butanol daun sumpit (Brucea javanica (L.) Merr.). Nilai IC50 untuk masing-masing sampel antara lain 152 ppm pada ekstrak kasar, 227 ppm pada ekstrak fraksi n-heksana, 91,12 ppm pada ekstrak fraksi etil asetat, dan 154,54 ppm pada ekstrak fraksi n-butanol. Dari pengujian aktivitas antioksidan diketahui bahwa ekstrak yang memberikan aktivitas antioksidan terbaik adalah ekstrak fraksi etil asetat.

2019 ◽  
Vol 3 (2) ◽  
pp. 29
Maria Eka Patri Yuliati ◽  
I Gusti Made Aman ◽  
Ni Nyoman Ayu Dewi

Introduction: Paraquat exposure causes aging because it induces oxidative stress marked by decreased level of SOD and increased MDA serum level. Macassar fruit contains bioactive compounds such as vitamin C, flavonoids, tannins and polyphenol that have antioxidant activity. The purpose of this study was to prove that Macassar fruit extract increased the level of Superoxide Dismutase (SOD) enzyme and reduce the level of Malodialdehid (MDA) in male Swiss Webster mice treated by paraquat. Methods: A randomized pretest-posttest control group study was conducted using 14 male mice which were 2-3 months old, healthy and had 25-30 gram in weight. They divided into 2 groups namely P0 (control) and P1 (Treatment). Both groups were treated by paraquat but only group P1 received 20 mg Macassar fruit extract while the P0 only got 1 cc placebo for 14 days. Results: Our result showed that the level of SOD was increased in P1 group from 17.18±1.69 U/ml to 67.56±3.65 U/ml (p<0.01) while no change was observed in P0 group 16.97 ±1.45U/ml to 17.07±1.89 U/ml (p>0.05). However, no effect on MDA level was observed as the level of MDA tended to slightly decrease in both groups. Conclusion: It can be concluded that Macassar fruit extract 20 mg/kgBB significantly increased the level of SOD whilehave no effect on MDA level in male mice Swiss Webster treated by paraquat.

2011 ◽  
Vol 81 (1) ◽  
pp. 43-48 ◽  
Rahime Bedir Findik ◽  
Nurcihan Karakurt Hascelik ◽  
Kadir Okhan Akin ◽  
Ayse Nurcan Unluer ◽  
Jale Karakaya

Background: Striae gravidarum, a clinical condition commonly seen in pregnant women, produces serious cosmetic problems and may lead to psychological problems. Aim: The present study investigated whether there was any relation between the presence of striae in primigravid pregnant women and blood vitamin C levels, and factors thought to contribute to the formation of striae such as family history, weight gained during pregnancy, smoking status, abdominal and thigh circumference, and age. Methods: Overall, 69 primigravid women attending routine antenatal follow-up and, using prophylactic iron and vitamin preparations, underwent investigation. All were pregnant 36 or more weeks. Scoring was based on striae examination and whether striae were present. The relation between the presence of striae, vitamin C blood levels, and other factors was investigated. Results and Conclusions: Multiple logistic regression analysis showed a significant relation between the presence of striae and blood vitamin C levels (p = 0.046) and between the presence of striae and family history (p = 0.023). No significant relation was found between the presence of striae and age, weight gained during pregnancy, abdominal and thigh circumference, or smoking status. It was concluded that further, more comprehensive studies on the issue are required.

2006 ◽  
Vol 76 (6) ◽  
pp. 359-366 ◽  
Rodríguez-Rodríguez ◽  
Ortega ◽  
López-Sobaler ◽  
Aparicio ◽  
Bermejo ◽  

This study investigated the relationship between the intake of antioxidant nutrients and the suffering of cataracts in 177 institutionalized elderly people (61 men and 116 women) aged ≥ 65 years. Dietary intake was monitored for 7 consecutive days using a "precise individual weighing" method. Subjects, who during their earlier years were exposed by their work to sunlight, had a greater risk of suffering cataracts (OR = 3.2; Cl: 1.1–9.3, P < 0.05) than those who worked indoors. A relationship was found between increased vitamin C intake and a reduced prevalence of cataracts (i.e., when comparing those above P95 for vitamin C intake with those below P5; (OR = 0.08; Cl: 0.01–0.75, P 0.05). Among subjects with cataracts, 12.1% had vitamin C intakes of < 61 mg/day (P10) and only 2.2% had intakes of > 183 mg/day (P95) (p < 0.01). Subjects who consumed > 3290 μg/day (P95) of lutein were less likely to have cataracts (OR = 0.086; Cl: 0.007–1.084; p < 0.05) than those whose consumption was < 256 μg/day (P5). In men, high intakes of zeaxanthin seemed to provide a protective effect against the problem (OR = 0.96; Cl: 0.91–0.99; p < 0.05). The results suggest an association exists between exposure to sunlight and the development of cataracts, and that vitamin C, lutein, and zeaxanthin offer some protection against this disorder.

2018 ◽  
Vol 88 (5-6) ◽  
pp. 263-269
Seong-Hoon Park ◽  
A Lum Han ◽  
Na-Hyung Kim ◽  
Sae-Ron Shin

Abstract. Background: Vitamin C is a strong antioxidant, and the health effects of vitamin C megadoses have not been validated despite the apparent health benefits. Therefore, the present study sought to confirm the effects of vitamin C megadoses. Materials and Methods : Four groups of six guinea pigs were used. Each group was fed one of the following diets for three weeks: normal diet, methionine choline-deficient diet, methionine choline-deficient diet + vitamin C megadose (MCD + vit C 2.5 g/kg/day), and methionine-choline deficient diet + ursodeoxycholic acid (MCD + UDCA 30 mg/kg/day). The MCD diet was given to induce nonalcoholic steatohepatitis, and UDCA was used to treat nonalcoholic steatohepatitis. Three weeks after initial diet administration, the results of biochemical tests and liver biopsy were compared between the groups. Results: The cytoplasm state was similar in the MCD + vit C and MCD + UDCA groups, exhibiting clearing of the cytoplasm and ballooning degeneration. However, macrovesicular steatosis was not observed in the MCD + vit C group. Aspartate transaminase and alanine transaminase were elevated significantly following vitamin C administration. Conclusions: The present study confirmed that alone vitamin C megadoses are potential remedies for nonalcoholic steatohepatitis, based on the liver biopsy results of guinea pigs that were unable to synthesize vitamin C.

2015 ◽  
Vol 72 (7) ◽  
pp. 463-468
Beat Bächi

Zusammenfassung. Künstliches Vitamin C ist heute ein Massenprodukt. Dass sich Ascorbinsäure seit ihrer Erfindung 1933 zu einem alltäglichen Konsumgut mausern konnte, ist weniger durch ihr medizinisches Potential zu erklären, sondern verdankt sich vielmehr einem dynamischen Zusammenspiel von Produktion, Vermarktung und Gesundheitspolitik. Der Beitrag fokussiert insbesondere auf die komplexen Interaktionen zwischen Gesundheitskonzepten, diagnostischen Instrumenten und ärztlichem Blick. Dabei scheinen nicht nur die Differenzen zwischen „rein naturwissenschaftlichen“ und „ärztlich-biologischen“ Standpunkten auf, sondern es zeigt sich auch, dass Nahrungsbestandteile immer auch gesundheitspolitisch und ethisch aufgeladen sind.

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