scholarly journals Noni (Morinda citrifolia L.) relationship analysis based on morphology character and random amplified polymorphic DNA (RAPD)

2021 ◽  
Author(s):  
R Heryanto ◽  
T Arlianti
Keyword(s):  
Random Amplified Polymorphic Dna ◽  
Morphological Characters ◽  
Fruit Shape ◽  
Fruit Weight ◽  
Morinda Citrifolia ◽  
Research Station ◽  
Relationship Analysis ◽  
Tropical Plant ◽  
Materials Used ◽  
Number Of Seeds

Noni (Morinda citrifolia L.) is a tropical plant with many benefits, one of which istraditional medicine. Moreover, noni can grow in all types of climates. Plant diversity is themain key in plant breeding. The existence of a diverse plant makes it easy for breeders toassemble varieties. This study aims to determine the diversity and relationship of nonigermplasm based on morphological characters and RAPD markers. The materials used ten noniaccessions at Cimanggu Research Station, Indonesian Spice and Medicinal Crops ResearchInstitute (ISMCRI), Bogor, West Java. The morphological characters observed were leaflength, leaf width, number of pods, fruit diameter, fruit weight, and number of seeds. Therewere 20 primers used for RAPD. The results showed that morphological characters of leafshape, fruit shape, and the number of seeds could distinguish noni. Molecular analysis showedthat three primers (OPA 9, OPA 17, and OPB 18) could produce polymorphic DNA bands.Based on differences in DNA band patterns, 10 noni accessions were divided into two groups.There is no specific DNA band pattern that distinguishes a total of seeds noni.

scholarly journals Phenotypic and genetic diversity of watermelon (Citrullus lanatus) in East Java, Indonesia

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
Achmad Amzeri ◽  
Kaswan Badami ◽  
Pawana Gita ◽  
Moh. Alfiyan Syah ◽  
Budi Setiadi Daryono
Keyword(s):  
Phenotypic Diversity ◽  
Citrullus Lanatus ◽  
Morphological Diversity ◽  
Morphological Characters ◽  
Morphological Characterization ◽  
Fruit Weight ◽  
Skin Thickness ◽  
Quantitative Characters ◽  
Number Of Seeds ◽  
Phenotypic And Genetic Diversity

Abstract. Amzeri A, Badami K, Pawana G, Alfiyan Syah M, Daryono BS. 2021. Phenotypic and genetic diversity of watermelon (Citrullus lanatus) in East Java, Indonesia. Biodiversitas 22: 5223-5230. The assembly of hybrid watermelon (Citrullus lanatus Thumb.) varieties with superior characters is an effort to meet the needs of watermelon seeds in Indonesia and reduce dependence on imports of watermelon seeds.  The morphological characterization of exploratory watermelon plants is needed to support the assembly of superior varieties because morphological characterization will reveal the characteristics of each watermelon tested.  In addition, the genetic and phenotypic diversity coefficients, heritability, and correlation between characters are needed to support the assembling of the desired variety.  This research aimed to determine morphological diversity, genetic and phenotypic diversity coefficients, heritability, and correlation among characters of watermelon from East Java, Indonesia.  The research used a randomized complete block design with ten genotypes as treatment and three replications,. The observed morphological characters were quantitative and qualitative characters.  The number of characters was 60 characters.  Eight quantitative characters, i.e., flowering date, harvesting date, fruit length, fruit diameter, skin thickness, fruit total soluble solids, number of seeds, and fruit weight were used to calculate the genetic and phenotypic diversity coefficient, heritability, and correlation between characters.  Quantitative character data were subjected to analysis of variance, followed with a Duncan Multiple Range Test (p<0.05).  The results showed that (i) Grouping based on morphological characters produces dendrograms with similarity coefficients ranging from 0.58 to 0.86 or there was a morphological diversity of 0.14 to 0.42, (ii) The phenotypic diversity coefficient (PDC) was greater than the genotypic diversity coefficient (PDC) in all observed quantitative characters, (iii) The broad sense Heritability values ?? of the tested watermelon genotypes ranged from 0.33 to 0.99, (iv) Fruit weight was significantly and positively correlated with fruit diameter, skin thickness, and number of seeds, (v) G1, G2, and G6 were the genotypes that can be used as parents to assemble superior watermelon varieties.

scholarly journals Genetic diversity of some apple cultivars in the south of Syria based on morphological characters

2015 ◽  
Vol 4 (4) ◽  
pp. 86-99 ◽  
Author(s):  
O. Al-Halabi ◽  
B. Muzher
Keyword(s):  
Cross Section ◽  
Leaf Shape ◽  
Morphological Characters ◽  
Fruit Shape ◽  
Mean Value ◽  
Fruit Weight ◽  
Leaf Margin ◽  
Mean Values ◽  
Apple Cultivars ◽  
Fruit Diameter

This work was aimed to evaluate genetic diversity among five local and six introduced apple cultivars in the germplasm which located in the South of Syria based on 29 morphological characters of leaf and fruit. The analysis of variance showed significant differences at P < 0.05 for all measured parameters, however the mean values of Limb length and width, fruit weight, length, and width indicated to a wide range of diversity between local and introduced cultivars, that the introduced cultivars have mostly the highest mean value while the local cultivars have the lowest mean values, that clearly found in the local apple cultivar Ksairi (K) which revealed the lowest mean value of fruit weight (FW), fruit length (FL) and fruit diameter (FD) in the comparison with all studied cultivars. Likewise, principal component analysis (PCA) was used and the first 4 principal components accounted for 76.4% of the total variance, with eigen values 29.2%, 18.8%, 16.9% and 11.5% respectively. The main important characters in PC1 are correlated with the high mean value of width of stalk cavity (WP), fruit weight (FW), fruit diameter (FD), fruit length (FL), core length (CL), width of calyx cavity (WC), core width (CW), depth of stalk cavity (DP), stalk diameter (SD), seed width (SeW) and leaf margin (LM). PC2 discriminated among cultivars depending on Fruit shape (FS), leaf shape (LS), over color (OC), maturity time (MT), core shape (CS), limp length (LL) and leaf base (LB) which were able to differentiate between all studied cultivars . PC3 is correlated with the high mean value of Stalk length (SL), titratable acidity (TA), total sugar (TS), and total soluble solids (TSS). PC4 is correlated with the high mean value of depth of calyx cavity (DC), leaf apices (LA) and fruit cross section (FCS). Cluster analysis was also used that divided the studied cultivars into two clusters, the first cluster included all local apple cultivars, in addition to Leaz Golden (LG) cultivar, however AbouGhabra (AG) and Ksairi (K) cultivars revealed the highest similarity, which have the same fruit shape(FS), background color (BC), core shape (CS), fruit cross section (FCS), leaf shape (Ls) and leaf margin (LM). While the second cluster included all the other introduced cultivars.International Journal of Environment Vol.4(4) 2015: 86-99

scholarly journals SPECIFIC FEATURES IN THE EXPRESSION OF INTRASPECIES VARIABILITY OF PRUNUS PUMILA FRUITS IN CHELYABINSK PROVINCE IN THE PROCESS OF INTRODUCTION

2019 ◽  
Vol 180 (3) ◽  
pp. 82-90
Author(s):  
M. S. Lezin ◽  
V. S. Simagin ◽  
A. V. Lokteva
Keyword(s):  
Natural Habitat ◽  
Morphological Characters ◽  
Fruit Shape ◽  
Fruit Weight ◽  
Natural Environments ◽  
Morphometric Traits ◽  
Intraspecies Variability ◽  
Peduncle Length ◽  
Almost All ◽  
Introduced Population

Background. The studied population of Prunus pumila L. has been introduced into Chelyabinsk Province, as its natural area of distribution is in North America. Of interest for researchers is the introduced population’s variability, induced by the effect of soil and climate factors natural for its new habitat.Materials and methods. A collection of 197 plants was employed to study the variability of P. pumila. Morphological characters were described in compliance with atlases dedicated to seed and fruit descriptive morphology, with some adjustments concerning specific features of the subfamily Prunoideae Focke. The confidence interval for means was calculated using the reliability criterion of t2 = 2.576. The degree of variation was assessed for the studied characters according to S. A. Mamaev’s scale.Results and conclusion. The average fruit weight was 2.49±0.11 g and varied from 1.25 to 5.15 g. The average stone weight was 0.25±0.01 g. Out of the number of morphometric traits, almost all linear characters of fruit and stone size had low variability (coefficient of variation [V]: 8–12%). Of those linear characters, only the peduncle length showed medium variability (V = 13–20%). The stone weight also had medium variability, while the fruit weight demonstrated high variability (V = 21–30%). The fruit shape was subglobose (40.3%), less frequently ovate (20.9%), ovoid (20.9%), broadly ovoid (9.7%), cordate (6.1%) or elliptic (2.0%). The ventral suture was usually barely noticeable, less frequently moderately (3.1%) or strongly expressed (1.0%). Polymorphism was observed in the pulp color, which was usually green-pink (72.8%). The P. pumila stone shape was elliptic (38.1%) or subglobose (34.0%), less frequently prolate (10.7%), ovoid (13.2%) or obovate (4.1%). The stone was symmetrically shaped from the ventral suture and sides, usually looking subglobose from the sides. The shape of the stone’s apex was rounded (85.3%) or, less frequently, obtusely mucronate (15.7%); its base was rounded (80.7%), often attenuate (15.7%), less frequently slightly slanted (3.6%). The stone surface texture was smooth (57.4%), often slightly costate (36.5%). There were several cases of a more costate surface of the stone (6.1%). Thus, the introduced population was found to have broad polymorphism of morphological characters in the fruit and the stone. A considerable increase was observed in morphometric indicators, including the size of fruit and stone, compared with those typical for the species in natural environments, which may be of special significance for breeders. The fusiform shape of the stone, characteristic of var. depressa Pursh. in its natural habitat, was absent in the introduced population.

scholarly journals Performance of hybrid lines of pointed gourd (Trichosanthes dioica Roxb) for yield and yield attributes

2018 ◽  
Vol 43 (3) ◽  
pp. 383-393
Author(s):  
N Ara ◽  
M Moniruzzaman ◽  
KS Rahman ◽  
M Moniruzzaman ◽  
R Sultana
Keyword(s):  
Agricultural Research ◽  
Block Design ◽  
Morphological Characters ◽  
Fruit Weight ◽  
Research Station ◽  
Leaf Margin ◽  
Yield Attributes ◽  
Pointed Gourd ◽  
Hybrid Lines ◽  
Light Green

A field experiment was conducted at the Regional Agricultural Research Station, Ishurdi, Pabna during the growing season of 2013-14 with eighteen hybrid lines of pointed gourd and BARI Hybrid Patal -1 as check to observe their performances for yield, yield attributes and other morphological characters. The experiment was laid out in randomized complete block design with three replications. The maximum vine length (242.00 cm) and nodes/plant (18.30) was recorded from PG018xM2. Shoots/plant ranged from 12.50 (PG027xM2) to 4.66 (PG008 M1). The maximum number of fruits/plant (160.00) was obtained from PG009xM2 followed by PG012xM1 (154.66). Individual fruit weight was recorded highest (50.10 g) in PG027xM2, which was very close to PG008xM2 (48.00) and PG018xM2 (47.00 g). Weight of fruits/plant ranged from PG009xM2 (6.86 kg) to PG022xM1 (3.01 kg). The highest pulp weight was recorded in PG027xM2 (44.20 g) which was statistically similar to PG008xM2 (42.20 g). Three different leaf colour (light green, green and deep green), two types of leaf tip (pointed and blunt) and four types of leaf margin (slightly serrated, serrated, entire and undulated) were found among the hybrid lines. Four fruit colour (whitish, light green, green and dark green), four fruit stripes (no stripe, white, green white and light green) and three types of fruit curvature (slightly curved, curved and straight) were observed in different lines. The line PG009xM2 showed better performance in respect of fruits/plant and weight of fruits/plant and thus gave the highest yield (45.74 t/ha). The lines PG008xM2, PG007xM2, PG017xM2, PG027xM2 and PG014xM1 also produced better yield (39.23 - 35.58 t/ha). Therefore, the lines PG014xM1, PG007xM2, PG008xM2, PG009xM2 and PG017xM2 should be subjected for further evaluation to release as variety.Bangladesh J. Agril. Res. 43(3): 383-393, September 2018

scholarly journals Preliminary Preliminary Evaluation of Morphological and Pomological Characters to Illustrate Genetic Diversity of Apricots (Prunus armeniaca L.) in Afghanistan

2020 ◽  
Vol 2 (5) ◽  
Author(s):  
Hedayatullah Salari ◽  
Alam Khan Samim ◽  
Saifuddin Ahadi ◽  
Sayed Ahmad Etemadi
Keyword(s):  
Genetic Diversity ◽  
Skin Color ◽  
Leaf Blade ◽  
Fruit Shape ◽  
Fruit Weight ◽  
Kernel Weight ◽  
Prunus Armeniaca ◽  
Soluble Solids ◽  
Research Station ◽  
Preliminary Evaluation

Afghanistan assumed to be a part of the diversity center of Central Asian group of apricots but due to lack of proper collection, precise detection and documentation of distinct variability, the apricot diversity in the country is under unknown and unprecedented threat. This study, therefore, was run in Badam-Bagh research station for two consecutive seasons (2016-2017) to assess 28 various characters of 20 native Afghan apricot accessions. Results showed a high variability among studied genotypes. Leaf and fruit characteristics such as leaf blade length, leaf blade width, fruit weight, kernel weight, mesocarp percentage, kernel bitterness and fruit quality like total soluble solids, pH, and titratable acidity, skin color and fruit shape which subsequently determines customer appeal and marketability, displayed huge variation. Based on fruit weight, there were two groups of apricot genotypes; small fruits (<37 g) and large fruits (>37 g). Accessions with large fruits embrace: “Amiri”, “Bid-Mushk”, “Hassan-Khili”, “Jibraelli”, “Koorpa”, “Qandak”, “Pir-Naqshi”, “Seyed-Abadi”, “Salari-Sorkh”, and “Saqi”. Most genotypes were early flowering and intermediately ripening in nature. Kernels of only “Walayati” accession had strong bitterness and remained were sweet-kernelled. The mesocarps of most accessions were very weakly adhered to stones. PCA showed 86.15% of total variance among genotypes to be due to first seven components. UPGMA cluster analysis separated 20 accessions into 2 main groups (13 accessions in the first and 7 accessions in the second groups). The results exhibited that morphological characters of leaf and fruit and pomological characters are of immense importance in fast and easy description of apricot genotypes. This is per se the first study on genetic diversity of apricot accessions performed in Afghanistan. The results would provide opportunity for apricot breeders to select accessions with elite and premium characters in terms of marketability and adaptability for particular growing sites.

scholarly journals GENETIC VARIABILITY OF INDONESIAN PAPAYA ACCESSIONS AS REVEALED BY RANDOM AMPLIFIED POLYMORPHIC DNA AND MORPHOLOGICAL CHARACTERIZATION

2019 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Riry Prihatini ◽  
Tri Budiyanti ◽  
Noflindawati Noflindawati
Keyword(s):  
Genetic Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Principal Component ◽  
Accurate Method ◽  
Morphological Characters ◽  
Morphological Characterization ◽  
Fruit Shape ◽  
Morphological Data ◽  
Group Method ◽  
Pair Group

<p class="abstrakinggris">Diverse papaya (<em>Carica</em> sp.) accessions are found in many regions in Indonesia, but their genetic diversity have not yet been studied. Random Amplified Polymorphic DNA (RAPD) is a simple yet accurate method that can be used to examine the genetic diversity of papaya. The study aimed to examine the genetic diversity of Indonesian papaya accessions using RAPD markers and morphological characters. The RAPD was applied on 23 papaya accessions using 30 primers. The appearing bands were further analyzed with the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) and Principal Component Analysis (PCA). The molecular results were then compared to the fruit morphological data, including fruit shape, size, flesh color, texture, and flavor. The RAPD analysis revealed that the 23 papaya accessions clustered into six main clades with Dice-Sorensen coefficient similarity ranged from 0.71 to 0.98. The first group consisted of 11 accessions, including both the hybrids and local accessions. The second group consisted of eight accessions especially six Indonesian hybrids, a Mexican Hybrid and a Hawaiian hybrid. The other four groups had a single member namely Sicincin Panjang, Lokal Sumani, Cariso, and Carica. The molecular grouping, however, did not align with the fruit character grouping. Overall, it was implied that the Indonesian papaya accessions were genetically narrow, of which some accessions were closely related to Hawaiian and Mexican accessions. These results can be used as a reference on papaya crossbreeding program in Indonesia.</p>

scholarly journals Study on genetic diversity of pointed gourd using morphological characters

1970 ◽  
Vol 33 (4) ◽  
pp. 607-616 ◽  
Author(s):  
ASMR Khan ◽  
MG Rabbani ◽  
MA Siddique ◽  
MI Hossain
Keyword(s):  
Genetic Diversity ◽  
Agricultural Research ◽  
Morphological Characters ◽  
Mean Value ◽  
Fruit Weight ◽  
Research Station ◽  
Cluster Distance ◽  
Total Divergence ◽  
Pointed Gourd ◽  
Five Axes

The genetic diversity among 64 pointed gourd genotypes were assessed through multivariate analysis from an experiment conducted in Regional Agricultural Research Station, lshurdi, Pabna during the growing season 2002-2003. The genotypes were grouped into twelve clusters. The cluster V consisted of highest number of genotypes and it was nine, the cluster VI and cluster VIII contained the lowest number of genotypes and it was two in each. The clustering pattern of the genotypes under this study revealed that the genotypes collected from the same location were grouped into different clusters. The genotypes of Jessore were distributed in different clusters. The highest inter genotype distance as 366.3 observed between the genotypes P0022 and P0007 and the lowest 2.6 as observed between the genotypes P0043 and P0044. Cluster V had the highest cluster mean value for internode length, fruit weight per plant and yield the highest inter-cluster distance was noticed between cluster III and II (45.71) and the lowest between cluster VII and VI (3.33). ‘The highest intra cluster distance was computed for cluster III and that was lowest for the cluster II. The first five axes accounted for 77.65% of the total variation among the 13 characters describing 64 pointed gourd genotypes. Fruit weight, seeds per fruit and fruit weight per plant contributed maximum to the total divergence. The results obtained by D2 analysis were confirmed by canonical analysis. Key Words: Diversity pointed gourd, morphological characters. doi: 10.3329/bjar.v33i4.2304 Bangladesh J. Agril. Res. 33(4) : 607-616, December 2008

scholarly journals ANALISIS KLASTER DURIAN (Durio zibethinus Murr.) UNGGUL LOKAL DI KABUPATEN JEMBER DAN BANYUWANGI

2021 ◽  
Vol 11 (2) ◽  
pp. 53
Author(s):  
Vega Kartika Sari ◽  
Eva Rosdiana ◽  
Sepdian Luri Asmono
Keyword(s):  
Water Content ◽  
Sugar Content ◽  
Morphological Characters ◽  
Fruit Weight ◽  
Leaf Length ◽  
Leaf Width ◽  
Durio Zibethinus ◽  
Chemical Content ◽  
Materials Used ◽  
Questionnaire Research

Indonesia is one of the centers of durian diversity in the world and one of the regions that makes durian a superior local fruit crop is Jember and Banyuwangi. Clustering is useful to facilitate the search for certain features of the type of durian. This study aims to determine the durian grouping in two districts namely Jember and Banyuwangi. The materials used were local superior durian fruit and characterization questionnaire. Research using descriptive methods. Characterization data will be analyzed using NTSYS software.The results showed that local superior durian fruit were grouped into 2 groups based on morphological characters. Cluster 1 consists of 4 durians namely Klemben, Hijau, Bajul, and Lambau. Cluster 2 only consists of durian Musang King. Based on the chemical content of durian fruit, durian Lambau from Jember has the highest water content of 75.52%, Musang King from Banyuwangi has a fat content (2.29%), Bajul from Jember has the highest fiber content (11.89%), and Hijau from Banyuwangi has the highest protein (3.14%) and sugar (26.08%) content. Based on PCA showed that Musang King and Hijau have similirity on some morphological characters and chemical fruit contents. The results of correlation analysis of morphological characters and chemical content of durian fruit showed that leaf length was positively correlated with leaf width and protein content. Leaf width is positively correlated with sugar content. Fruit weight is positively correlated with water content.

VARIATION AMONG BRITISH COLUMBIA AND NORTHERN ALBERTA POPULATIONS OF RASPBERRIES, RUBUS IDAEUS SUBSPP. STRIGOSUS MICHX.

1972 ◽  
Vol 52 (6) ◽  
pp. 1067-1072 ◽  
Author(s):  
M. C. J. VAN ADRICHEM
Keyword(s):  
British Columbia ◽  
Seed Weight ◽  
Fruit Weight ◽  
Winter Hardiness ◽  
Rubus Idaeus ◽  
Specific Character ◽  
Positive Correlation ◽  
Total Seed ◽  
Northern Alberta ◽  
Number Of Seeds

Several population samples of Rubus idaeus subspp. strigosus Michx. (raspberries) were collected in British Columbia and northern Alberta and a number of characters were studied and compared with the cultivar Trent. Significant differences between populations were found for cane length, number of buds per cane, percentage of buds growing, number of inflorescences and flowers per bud, fruit and seed weight. There was a positive correlation between the total seed weight and the number of seeds, and between the total seed and fruit weight. No distinct types could be established based on location or elevation. None of the populations showed significantly better winter hardiness than the cultivar Trent, and although some had a specific character that was superior, none was found to have overall superiority.

scholarly journals ASSOCIATIONS BETWEEN TRAITS IN FISÁLIS: A TOOL FOR INDIRECT SELECTION OF SUPERIOR PLANTS

2017 ◽  
Vol 39 (4) ◽  
Author(s):  
NICOLE TREVISANI ◽  
RITA CAROLINA DE MELO ◽  
MAURO PORTO COLLI ◽  
JEFFERSON LUÍS MEIRELLES COIMBRA ◽  
ALTAMIR FREDERICO GUIDOLIN
Keyword(s):  
Path Analysis ◽  
Pearson Correlation ◽  
Block Design ◽  
Correlation Coefficients ◽  
Indirect Selection ◽  
Fruit Weight ◽  
Physalis Peruviana ◽  
Randomized Block Design ◽  
Number Of Seeds ◽  
Fruit Diameter

ABSTRACT Knowledge about associations between traits is fundamental for plant breeding, since indirect selection can accelerate the development of promising genotypes. This study assessed the magnitude of associations between agronomically important traits in fisális (Physalis peruviana L). The experiment was conducted in Lages, Santa Catarina, and the treatments consisted of six fisális populations. The experiment was arranged in a randomized block design, with two replications and seven plants per plot. The correlations between traits were estimated based on the Pearson correlation coefficients and partitioned into direct and indirect effects through path analysis. Fruit weight was positively correlated with number of seeds (0.874), equatorial fruit diameter (0.738) and polar fruit diameter (0.672). By path analysis, number of seeds was identified as the trait with the highest direct contribution to fruit weight. The high phenotypic correlations between the equatorial and polar fruit diameters with fruit weight were mainly due to the indirect effect via number of seeds (0.505 and 0.459). The selection for heavier fisális fruits was strongly influenced by number of seeds, i.e., this trait should be taken into account for selection.

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