Human gingival fibroblasts proliferation and attachment to different novel implant-abutment materials: an in vitro study

2018 ◽  
Author(s):  
Ahmed Rozeik
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Implant Abutment

scholarly journals In vitro study of surface alterations to polyetheretherketone and titanium and their effect upon human gingival fibroblasts

2021 ◽  
Vol 125 (1) ◽  
pp. 155-164 ◽  
Author(s):  
Maryam Gheisarifar ◽  
Geoffrey A. Thompson ◽  
Carl Drago ◽  
Fahimeh Tabatabaei ◽  
Morteza Rasoulianboroujeni
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts

scholarly journals Evaluation of the Biological Behavior of Mucograft® in Human Gingival Fibroblasts: An In Vitro Study

2015 ◽  
Vol 26 (6) ◽  
pp. 602-606 ◽  
Author(s):  
Rafaela S. R. e Lima ◽  
Daiane C. Peruzzo ◽  
Marcelo H. Napimoga ◽  
Eduardo Saba-Chujfi ◽  
Silvio Antonio dos Santos-Pereira ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
In Vitro Study ◽  
Vitro Study ◽  
Human Gingival Fibroblasts ◽  
Soft Tissue Augmentation ◽  
Biological Behavior ◽  
Type Iii Collagen ◽  
Gingival Fibroblasts ◽  
Type Iii

Mucograft(r) is a resorbing porcine matrix composed of type I and type III collagen, used for soft tissue augmentation in guided tissue bony regeneration procedures. This in vitro study aimed to evaluate the biological behavior of Mucograft(r) in human gingival fibroblasts, as well as the ability of the matrix to induce production of extracellular matrix. Six resorbing Mucograft(r) matrices (MCG) were cut into 3 x 2 mm rectangles and 5 x 5 mm squares and were placed in 96- and 24-well plates, respectively. The control group (CTRL) consisted of cells plated on polystyrene without the MCG. After one, two, three and seven days, cell proliferation and viability were assessed using the Trypan exclusion method and MTT test, respectively. Type III collagen (COL 3A1) and vimentin (VIM) expression were also evaluated at 10 and 14 days, using Western blotting. Statistical analysis, using ANOVA with post hoc Bonferroni test, revealed that human gingival fibroblasts from MCG showed similar results (p>0.05) for proliferation and viability as the cells cultured on CTRL. After 14 days, a significant decrease in COL 3A1 expression (p<0.05) was observed when cultured with the MCG. VIM expression showed no significant difference at any time period (p>0.05). Although no increase in extracellular matrix secretion was observed in this in vitro study, Mucograft(r) presented cellular compatibility, being an option for a scaffold whenever it is required.

Comparison of the effect of root surface modification with citric acid, EDTA, and aPDT on adhesion and proliferation of human gingival fibroblasts and osteoblasts: an in vitro study

2017 ◽  
Vol 33 (3) ◽  
pp. 533-538 ◽  
Author(s):  
Rafael Ferreira ◽  
Renato Taddei de Toledo Barros ◽  
Paula Stephania Brandão Hage Karam ◽  
Adriana Campos Passanezi Sant’Ana ◽  
Sebastião Luiz Aguiar Greghi ◽  
...  
Keyword(s):  
Surface Modification ◽  
Citric Acid ◽  
In Vitro Study ◽  
Root Surface ◽  
Vitro Study ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts
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