scholarly journals Reaction of Sulfur and Sustainable Algae Oil for Polymer Synthesis and Enrichment of Saturated Triglycerides

2021 ◽  
Author(s):  
Adarsha Gupta ◽  
Max Worthington ◽  
Munish Puri ◽  
Justin Chalker
Keyword(s):  
Single Cell ◽  
Environmental Remediation ◽  
Downstream Processing ◽  
Single Step ◽  
Biodiesel Production ◽  
Direct Reaction ◽  
Oil Extract ◽  
Algae Oil ◽  
Novel Strategy ◽  
Unsaturated Triglycerides

There is growing interest in the bio-based production of lipids from algae. These lipids have a range of uses including nutritional supplements and precursors to biodiesel. Single-cell thraustochytrids are especially attractive in this regard in that they can produce over 50% of their weight as triglycerides. Furthermore, the distribution of saturated and unsaturated triglycerides can be modulated by changes in strain variation and modulation of fermentation conditions. Nonetheless, there remains a need for versatile downstream processing to enrich these so-called “single cell oils” into classes based on degree of unsaturation. In this study, we report a novel strategy for enriching saturated triglycerides produced in thraustochytrids. The method features direct reaction of elemental sulfur with the algae oil extract. The sulfur copolymerizes with >90% of the unsaturated triglycerides, providing a new route to a class of materials previously used in environmental remediation, Li-S battery cathodes, slow-release fertilisers, and insulation. The unreacted oil is enriched in saturated triglycerides, which can be isolated by extraction for potential use in biodiesel production. In this way, a single batch of sustainably produced algae oil can be converted into multiple useful products in a single step.

scholarly journals The Potential of Single-Cell Oils Derived From Filamentous Fungi as Alternative Feedstock Sources for Biodiesel Production

2021 ◽  
Vol 12 ◽  
Author(s):  
Sizwe I. Mhlongo ◽  
Obinna T. Ezeokoli ◽  
Ashira Roopnarain ◽  
Busiswa Ndaba ◽  
Patrick T. Sekoai ◽  
...  
Keyword(s):  
Single Cell ◽  
Filamentous Fungi ◽  
Process Integration ◽  
Scale Up ◽  
Recovery Process ◽  
Downstream Processing ◽  
Production Costs ◽  
Biodiesel Production ◽  
Industrial Processing ◽  
Single Cell Oils

Microbial lipids, also known as single-cell oils (SCOs), are highly attractive feedstocks for biodiesel production due to their fast production rates, minimal labor requirements, independence from seasonal and climatic changes, and ease of scale-up for industrial processing. Among the SCO producers, the less explored filamentous fungi (molds) exhibit desirable features such as a repertoire of hydrolyzing enzymes and a unique pellet morphology that facilitates downstream harvesting. Although several oleaginous filamentous fungi have been identified and explored for SCO production, high production costs and technical difficulties still make the process less attractive compared to conventional lipid sources for biodiesel production. This review aims to highlight the ability of filamentous fungi to hydrolyze various organic wastes for SCO production and explore current strategies to enhance the efficiency and cost-effectiveness of the SCO production and recovery process. The review also highlights the mechanisms and components governing lipogenic pathways, which can inform the rational designs of processing conditions and metabolic engineering efforts for increasing the quality and accumulation of lipids in filamentous fungi. Furthermore, we describe other process integration strategies such as the co-production with hydrogen using advanced fermentation processes as a step toward a biorefinery process. These innovative approaches allow for integrating upstream and downstream processing units, thus resulting in an efficient and cost-effective method of simultaneous SCO production and utilization for biodiesel production.

Single cell oil and its application for biodiesel production

2017 ◽  
Vol 111 ◽  
pp. 747-756 ◽  
Author(s):  
M. Madani ◽  
M. Enshaeieh ◽  
A. Abdoli
Keyword(s):  
Single Cell ◽  
Biodiesel Production ◽  
Single Cell Oil

Synthesis of single-cell oil by Yarrowia lipolytica MTCC 9520 utilizing slaughterhouse lipid waste for biodiesel production

2020 ◽  
Author(s):  
P. Radha ◽  
Sanjana Narayanan ◽  
Angana Chaudhuri ◽  
Sameena Anjum ◽  
Deborah Lilly Thomas ◽  
...  
Keyword(s):  
Single Cell ◽  
Yarrowia Lipolytica ◽  
Biodiesel Production ◽  
Single Cell Oil

Single step synthesis of sulfonic group bearing graphene oxide: A promising carbo-nano material for biodiesel production

2016 ◽  
Vol 4 (3) ◽  
pp. 2933-2940 ◽  
Author(s):  
Triveni Kumar Mahto ◽  
Rajat Jain ◽  
Soumen Chandra ◽  
Dhrubojyoti Roy ◽  
Vikas Mahto ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Single Step ◽  
Biodiesel Production ◽  
Sulfonic Group ◽  
Nano Material

scholarly journals Activated Charcoal—A Potential Material in Glucoamylase Recovery

2011 ◽  
Vol 2011 ◽  
pp. 1-4 ◽  
Author(s):  
S. O. Kareem ◽  
I. Akpan ◽  
T. O. S. Popoola ◽  
L. O. Sanni
Keyword(s):  
Molecular Weight ◽  
Activated Charcoal ◽  
Contact Time ◽  
Enzyme Purification ◽  
Downstream Processing ◽  
Single Step ◽  
Major Protein ◽  
Rhizopus Oligosporus ◽  
Sds Page ◽  
Effects Of Ph

The potential of activated charcoal in the purification of fungal glucoamylase was investigated. Various concentrations of activated charcoal (1–4% w/v) were used to concentrate crude glucoamylase from Rhizopus oligosporus at different temperature values (30–50°C). Effects of pH (3.0–6.0) and contact time (0–60 min) on enzyme purification were also monitored. Activated charcoal (3% w/v) gave a 16-fold purification in a single-step purification at 50°C for 20 min and pH 5.5. The result of SDS-PAGE analysis of purified glucoamylase showed two major protein bands with corresponding molecular weight of 36 kDa and 50 kDa. The method is inexpensive, rapid, and simple which could facilitate downstream processing of industrial enzyme.

scholarly journals Spectroscopic Analysis of Heterogeneous Biocatalysts for Biodiesel Production from Expired Sunflower Cooking Oil

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Enoch Wembabazi ◽  
Patrick Joram Mugisha ◽  
Asumani Ratibu ◽  
Deborah Wendiro ◽  
Joseph Kyambadde ◽  
...  
Keyword(s):  
Calcium Oxide ◽  
Sunflower Oil ◽  
Active Form ◽  
Single Step ◽  
Biodiesel Production ◽  
Saw Dust ◽  
Heterogeneous Biocatalysts ◽  
Egg Shells ◽  
Heterogeneous Biocatalyst

The study characterized heterogeneous biocatalyst synthesized from sucrose, saw dust, and chicken egg shells using Fourier Transform Infrared (FTIR) spectroscopy coupled with Attenuated Total Reflectance (ATR) technique. Acidic sulphonate (–SO3H) groups were more visible in the spectrum generated for carbonized and sulphonated sucrose than in carbonized and sulphonated saw dust. This was highlighted further by the significantly higher conversion percentage achieved for sulphonated sucrose (62.5%) than sulphonated saw dust (46.6%) during esterification of expired sunflower oil (p=0.05). The spectra for calcinated egg shells also showed that the most active form of calcium oxide was produced at calcination temperature of 1000°C. This was confirmed in the single-step transesterification reaction in which calcium oxide generated at 1000°C yielded the highest biodiesel (87.8%) from expired sunflower oil. The study further demonstrated the versatility of the FTIR technique in qualitative analysis of biodiesel and regular diesel by confirming the presence of specific characteristic peaks of diagnostic importance. These findings therefore highlight the potential of FTIR-ATR as an inexpensive, fast, and accurate diagnostic means for easy identification and characterization of different materials and products.

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