scholarly journals Assessment of bioscompatibility of isocyanurate-containing polyurethanes with ifosphamide by in vivo and in vitro methods.

Morphologia ◽  
2021 ◽  
Vol 15 (1) ◽  
pp. 39-47
Author(s):  
N.A. Galatenko ◽  
D.V. Kuliesh ◽  
R.A. Rozhnova ◽  
V.P. Gritsenko ◽  
L.F. Narazhayko
Keyword(s):  
Tissue Culture ◽  
Composite Materials ◽  
Polymeric Composite ◽  
Culture Method ◽  
Experimental Animals ◽  
Polymeric Composite Materials ◽  
In Vitro Methods ◽  
Tissue Culture Method

Background. The creation of polymeric composite materials with pronounced biological activity, which are able to act as implants with local prolonged action of the immobilized substance can be widely used in medical practice. Objective. Study of cellular reactions of surrounding tissues of experimental animals to implantation of polymeric composite materials based on isocyanurate-containing polyurethanes with ifosfamide, study of histotoxicity of the obtained materials by tissue culture method. Methods. Polymeric composite materials based on isocyanate-containing polyurethanes without and with ifosfamide were implanted into the body of white laboratory Wistar rats. Cellular responses of the organism and possible changes in the structure of test specimens after implantation were studied by light microscopy by analysis of histological micropreparations. In order to study the peculiarities of the dynamics of growth and development of fibroblastic elements, the method of tissue culture was used. Results. Conducted biological studies by in vivo and in vitro methods allowed to evaluate the effect of immobilized ifosfamide in the structure of isocyanurate-containing polyurethanes on cellular reactions of surrounding tissues during implantation in experimental animals, as well as the effect of extracts from developed polymer samples on cultured cell growth. Conclusion. It was found that the implantation of isocyanurate-containing polyurethanes with ifosfamide led to the development of intense cellular reactions in the area of implant placement, primarily the reaction of round cell elements. The presence of ifosfamide in the structure of the polymeric implantation material probably affected the proliferation of cellular elements, inhibited regenerative processes in the early stages of the study and delayed the formation of a mature connective tissue capsule around the implanted samples. The tissue culture method showed that when making an extract of isocyanurate-containing polyurethanes with ifosfamide in the culture medium, there was a large variability of cell forms, which led to the appearance of macrophage-like elements.

scholarly journals In Vitro Effect of Erythropoietin on the Spleen of The Polycythemic Mouse

Blood ◽  
1967 ◽  
Vol 29 (6) ◽  
pp. 852-858
Author(s):  
YASUSADA MIURA ◽  
FUMIMARO TAKAKU ◽  
KIKU NAKAO
Keyword(s):  
Tissue Culture ◽  
Culture Method ◽  
Stem Cell Differentiation ◽  
Mouse Spleen ◽  
In Vitro Method ◽  
Heme Synthesis ◽  
Tissue Culture Method ◽  
Vitro Effect

Abstract 1. An in vitro method to observe radiosensitivity of stem cells was developed in the present study. In vivo and in vitro effect of 60Co irradiation on the erythropoietin-induced stem cell differentiation into erythroblasts was observed, using a tissue culture method of polycythemic mouse spleen. Response to erythropoietin was demonstrated by an appearance of heme synthesis and erythroblasts in spleen fragments. 2. A significant correlation between the rate of appearance of erythroblasts and heme synthesis of the spleen fragments was observed. 3. After irradiation, marked impairment of both heme synthesis and production of erythroblasts was observed, yielding D37 values in the vicinity of 70 r in vivo and 120 r in vitro irradiation, respectively. 4. Marked recovery of erythropoietin-induced heme synthesis in the polycythemic mouse spleen was observed 9 days after 300 r irradiation, with an "overshooting" phenomenon on the 12th day.

Toxin action b. Novyi (b. Oedematiens Weinberg-Sequin) on tissue growth in vitro and on cell metabolism. To the study of anaerobes and their toxin using the explantation method

1935 ◽  
Vol 31 (2) ◽  
pp. 271-272
Author(s):  
G. Barg
Keyword(s):  
Tissue Culture ◽  
Cell Metabolism ◽  
Anaerobic Bacteria ◽  
Culture Method ◽  
Tissue Growth ◽  
Tissue Culture Method ◽  
Series Of Experiments

The author has shown in a series of experiments that the tissue culture method is quite applicable for the study of anaerobic bacteria and their toxins, especially those of them that are significantly weakened by filtration.

scholarly journals Usage of the tissue culture method for the development of new forms of Fagopyrum esculentum Moench

2019 ◽  
pp. 3-6 ◽  
Author(s):  
E. N. Barsukova ◽  
A. G. Klykov ◽  
E. L. Chikina
Keyword(s):  
Heavy Metal ◽  
Tissue Culture ◽  
Copper Ions ◽  
Culture Method ◽  
Fagopyrum Esculentum ◽  
Tissue Culture Method ◽  
Fagopyrum Esculentum Moench ◽  
Effect Of Copper ◽  
Positive Effect

The article presents results of usage of the tissue culture method on buckwheat (Fagopyrum esculentum Moench) together with the selective factor increased concentrations of heavy metal ions in the nutrient medium. On the basis of Izumrud (Russia), Kitavase (Japan), hybrid Izumrud x Kitavase there were developed regenerant lines tolerant to copper and zinc. Negative action effect of copper ions was observed from the second generation in the second posterity of the plants-regenerants, developed from the callus, in the form of the chlorophyll-free mutants. Positive effect of the heavy metals was observed on increasing of rutin content of the plants-regenerants in vitro. Selective evaluation in the field conditions of the samples tolerant to heavy metal let to choose from them the most adaptive to monsoon conditions in Primorskykrai, which combine valuable traits of big grain (weight of 1000 grains 36-38 g), high seed productivity (1,9-3,2 g) and high rutin content in aboveground mass of plants (2,8-4,3 %).

Development of explanted rat embryos in circulating medium

Development ◽  
1967 ◽  
Vol 17 (3) ◽  
pp. 513-525
Author(s):  
D. A. T. New
Keyword(s):  
Tissue Culture ◽  
Culture Method ◽  
Rat Embryos ◽  
Embryo Extract ◽  
Tissue Culture Method

The inaccessibility of rat embryos in vivo has prompted several previous attempts to grow them in culture. It has been shown that embryos explanted at head fold or early somite stages with their membranes will develop on the surface of plasma-embryo extract clots (New & Stein, 1964; New, 1966 a) or in homologous liquid plasma or serum (Nicholas & Rudnick, 1934, 1938; Jolly & Lieure, 1938; New, 1966a, b). The explants can conveniently be cultured in watch glasses, as in the standard tissue culture method of Fell & Robison (1929), and development is improved if they are incubated in 60–90% O2 and 3–5% CO2. Under these conditions the older embryos grow well for 30–40 h developing to 25- to 30-somite stages. However, no embryos have been observed to develop much further than this, and those explanted at stages more advanced than 30 somites have always died fairly quickly.

scholarly journals MICROPROPOGATION AND ANALYSIS OF PHYTOCHEMICAL PROFILE OF TISSUE CULTURE GROWN PLANTAGO OVATA FORSK.

2017 ◽  
Vol 10 (4) ◽  
pp. 202 ◽  
Author(s):  
Mamta Sharma ◽  
Amita Kumari ◽  
Eshita Mahant
Keyword(s):  
Tissue Culture ◽  
Ecological Factors ◽  
Culture Method ◽  
Culture Conditions ◽  
Culture Technique ◽  
Plantago Ovata ◽  
Tissue Culture Technique ◽  
Grown Plant

Objectives : Plantago ovata is an important medicinal plant of Himalayan region greatly used in herbal dugs manufacturing. The plant is multipurpose and strictly present in the Himalaya. Plantago has many medicinal properties such as antioxidant, anti-inflammatory and hematopoiesis effects and protects the liver and is used for the treatment of cancer. The plant being medicinal possesses complex phytochemicals. The investigation of various Plantago organ (leaves, stem etc) revealed their high potential to produce a wide array of bioactive secondary metabolites. In present study the a new method of micropropagation through tissue culture  was developed for Plantago so as to meet the future demand of plant. Futher a morphological and physiochemical comparison of tissue culture grown plant was done with in vivo grown plants.Methods:  Plantago ovata was grown in -vitro through tissue culture technique using MS media and in-vivo in the nursery area of Shoolini University. In vitro culture of  Plantago ovata forsk. were managed to restrict the ecological factors and to control the culture conditions. Experimental culture parameter including germination and phytochemical constituents of Plantago ovata in vivo and in vitro conditions were observed.Results: The result revealed changes in the concentration of phytochemical constituent’s in tissue culture grown Plantago. Phytochemicals constituents (carbohydrate, tannin, chlorophyll, saponin) was reduced in tissue culture grown plant where as some phytochemicals (phenol, alkaloid, flavanoid, protein, phytosterol) increased in tissue culture grown plant than in vivo plant.  A reduction in morphological trait was found in tissue cultured plant.Conclusion: The developed tissue culture method for the micropropagation of  Plantago ovata can be used as milestone to meet the industrial need in near future.Keywords: Plantago, Tissue Culture Technique, germination, phytochemicals.

scholarly journals Genotypic variability of Pinus sylvestris L. on the drought-resistance attribute

2019 ◽  
Vol 23 (1) ◽  
pp. 15-23
Author(s):  
E. Yu. Amineva ◽  
A. P. Gureev ◽  
T. M. Tabatskaya ◽  
O. S. Mashkina ◽  
V. N. Popov
Keyword(s):  
Gene Expression ◽  
Tissue Culture ◽  
Drought Resistance ◽  
Culture Method ◽  
Callus Cultures ◽  
Seed Progeny ◽  
Tissue Culture Method ◽  
Drought Resistant ◽  
Resistant Genotypes

There was an increase and intensification of droughts in connection with global climate change in recent decades. Not all plant organisms are able to adapt to changing environmental conditions. Therefore, the question of stressresistant (drought-resistant) genotypes selection for breeding is quite urgent. This problem also concerns forest tree plants, including Scots pine, which is one of the main foresters in the Voronezh region. The results of vegetative and generative sphere analysis of individual Scotch pine trees with the help of biotechnology, molecular-genetic and cytogenetic method are given in this study. The possibility of applying the tissue culture method in vitro for testing initial plants for stress resistance, including drought resistance, is explained by the interconnection of cells, tissues and the whole plant properties. It is shown that the cytogenetic characteristics of the seed progeny and the indicators of callusogenic reactions do not always coincide in different genotypes: in some cases energy resources are spent on ontogeny protection, while, in other cases, to reproductive function maintain. There are trees, in which the state the generative sphere in arid years is at the level of optimal years, and their callus cultures reaction remains unchanged even under simulated conditions of drought. Based on the results obtained for the selection of Scots pine drought-resistant genotypes we suggest applying a system of criteria characterizing both the ability of the vegetative sphere to survive in drought conditions on the basis of the tissue culture method in vitro (callus tissue formation speed, its viability, frequency of callusogenesis), and the state of the generative sphere with the help of cytogenetic analysis of seed progeny (frequency of mitosis pathologies, the proportion of cells with micronuclei, mitotic activity). The expediency of applying the biotechnological approach was proved by analysis of the gene expression level of stress proteins: the level of AbaH gene expression correlates greatly with the indicator of the viability of callus cultures, including ones on a nutrient medium with an additional stress agent (NaCl). Trees that can be assigned to drought-resistant ones, according to the results of the analysis, should be recommended for use in breeding.

scholarly journals 65 years of in vitro culture in Poland

2013 ◽  
Vol 82 (3) ◽  
pp. 183-192
Author(s):  
Maciej Zenkteler ◽  
Elżbieta Zenkteler
Keyword(s):  
Tissue Culture ◽  
In Vitro Culture ◽  
Plant Disease Resistance ◽  
Culture Method ◽  
Short Review ◽  
Meristem Culture ◽  
Biological Processes ◽  
Practical Applications ◽  
Tissue Culture Method

This paper is a short review of historical development of the tissue culture method in Poland. Similarly to the rest of the world, in vitro technology in Poland has progressed in many directions simultaneously. Its main fields are closely interconnected by natural sequences of biological processes and integrade one into another. The best results, driven by the prospects of practical applications, have been achieved within the areas of meristem culture and micropropagation of valuable genotypes, somaclonal variation, production of haploids and DH plants, somatic embryogenesis, in vitro culture of embryos and ovules, improvement of plant disease resistance, somatic hybridization and transformation of plants. Due to the fact that in vitro domain is a very broad science the authors are aware that this review might not fully cover all the scientists working with tissue culture and their achievements.

scholarly journals Microtubers production by using Temporary Immersion System (TIS) bioreactor to potato varieties

2021 ◽  
Vol 886 (1) ◽  
pp. 012005
Author(s):  
Septi Andriani ◽  
Luthfi Aziz Mahmud Siregar ◽  
Irda Safni
Keyword(s):  
Tissue Culture ◽  
Culture Method ◽  
Potato Seed ◽  
Temporary Immersion System ◽  
Temporary Immersion ◽  
Potato Varieties ◽  
Randomized Design ◽  
Tissue Culture Method ◽  
Certified Potato Seed

Abstract As the national potatoes growth has become lower from year to year, it is now known that the problems came from the lack of certified potato seed varieties and the minimum access to sophisticated technology to make a good potato variety. The solution that can be made is to utilize microtubers as an efficient factor. The purpose of this research is to find the most effective method in micro tubers cultivation in vitro by comparing the conventional tissue culture method and the Temporary Immersion System (TIS) bioreactor method to four different potato varieties (Granola L., Dayang Sumbi, Atlantic Malang, Maglia). This research uses a split-plot design with a completely randomized design by using two factorial. The result of this study shows that the microtubers in the multiplication and production step using the TIS bioreactor method has a higher average compared to the conventional tissue culture method. As the various details, Dayang Sumbi has the highest parameter such as most sprouts, primer roots, diameter, wet weights, and fastest time growth. Granola L. excel in planlet height and most tubers. Atlantic Malang in the most multiplication and nodus. Meanwhile Maglia excel in most leaves.

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