scholarly journals IMPACT OF COVID-19 ON THE MICROBIAL LOAD OF MUD CRAB, SCYLLA SERRATA: A STUDY FROM SOUTH 24 PARGANAS DISTRICT OF WEST BENGAL, INDIA

Author(s):  
Sana Ahmed

This study was conducted to evaluate the microbial load in the flesh and gills of the mud crab (Scylla serrata) collected from the Nagendra Bazar area in Diamond Harbour in South 24 Parganas district of West Bengal, India. The period of collection coincided with the Pre-COVID-19 phase (June 2018 and June 2019) and the COVID lockdown phase (June 2020 and June 2021). It is observed that the total coliform loads in both muscle and gills are more in the pre-COVID phase compared to the COVID lockdown phase. ANOVA analysis also confirmed significant variations between years and body parts (muscle and gills) for both Total coliform (TC) and Fecal Coliform (FC) (p < 0.01). The present study is of considerable importance as crabs are consumed by local people as well as exported in several foreign countries like China, Singapore, Japan, and the U.S.A.

2013 ◽  
Vol 5 (3) ◽  
pp. 545-553 ◽  
Author(s):  
M. G. Sarower ◽  
S. Bilkis ◽  
M. A. Rauf ◽  
M. Khanom ◽  
M. S. Islam

The biochemical composition (proteins, lipid, ash and moisture) of different parts of the body (gill, meat, egg) of fattened and natural mud crab Scylla serrata was determined. Twenty four samples of S. serrata (12 from wild & 12 from fattening farm) of different sizes and sexes (half of the sample is male) were used in this study. The mean protein and lipid contents are significantly (p<0.05) higher in fattened crabs than natural in natural crabs regardless of size and sexes.  Moisture and ash were higher in natural crab than in fattened ones. The highest and lowest moisture content was noticed in gills and eggs in both natural and fattened crabs. Protein and lipid contents were comparatively higher in eggs than in other body parts. Female crabs contained high protein and lipid than males in both natural and fattened crabs.  From the results of this study it may considered that fattened crab might be comparatively superior to natural crabs as they appear to provide higher levels of protein and fat  for human nutrition. Keywords: Biochemical composition; Natural and Fattened mud crab; Scylla serrata   © 2013 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: http://dx.doi.org/10.3329/jsr.v5i3.14082 J. Sci. Res. 5 (3), 545-553 (2013)


Author(s):  
Pallavi Dutta

Microbial load in terms of Total Coliform (TC) and Fecal Coliform (FC) were documented in the water of a shrimp culture farm at Malancha region of North 24 Parganas for a period of 36 years (1984-2019). The region receives the wastewater from the city of Kolkata. A steady hike in the microbial load (comprising of both total and fecal coliform) is noticed. The primary reason behind this rise of the microbial load is the run-off from the nearby landmasses that brings various types of wastes in the shrimp farm under investigation. The sustainability of shrimp farms in this region is under question due to the huge microbial load as revealed from the output of NAR.


2000 ◽  
Vol 43 ◽  
pp. 233-239 ◽  
Author(s):  
LE Andersen ◽  
JH Norton ◽  
NH Levy

2015 ◽  
Vol 12 (1) ◽  
pp. 1 ◽  
Author(s):  
Rosmilah Misnan ◽  
Nurul Izzah Abdul Rahman ◽  
Zailatul Hani Mohd Yadzir ◽  
Noormalin Abdullah ◽  
Mohd Faizal Bakhtiar ◽  
...  

Crab meat is widely consumed in several countries around the world. However, when consumed, crab meats are frequent cause of allergic reactions throughout the world. Scylla serrata is among the most common mud crab in Malaysia. In a previous study two major allergens of mud crab at 36 and 41 kDa was identified. Thus, the aim of this study is to further identify these major allergens by a proteomic approach. Protein extract was prepared and resolved by 2-dimensional electrophoresis (2-DE). Immunoblotting was then performed using reactive sera from patients with crab allergy. Major allergenic spots were then excised from the 2-DE gel and analysed by mass spectrometry. The 2-DE profile of the extract revealed approximately >100 protein spots between pH of 4.00 to 8.00. Mass spectrometry analysis has identified the 36 and 41 kDa proteins as tropomyosin and arginine kinase, respectively. Our findings indicated that tropomyosin and arginine kinase play a major role in allergic reaction to mud crab meat among local patients with crab meat allergy, and should be included in diagnostics and therapeutic strategies of this allergy.


1995 ◽  
Vol 31 (12) ◽  
pp. 239-248 ◽  
Author(s):  
Ana Emparanza-Knörr ◽  
Francisco Torrella

The Salmonella presence and the microbiological quality indicators, total and fecal coliforms and coliphages of E. coli C, have been studied in a overloaded wastewater lagoon system treating urban wastewatrers of the village of Guardamar del Segura (Alicante, Spain). Classical microbiological technology to detect salmonellae was used, including pre-enrichment, enrichment, selective media plating and biochemical and serological confirmation. Water was physicochemically characterized according to COD, SS, temperature, pH and dissolved oxygen. The selective migration step through Rappaport-Vassiliadis semisolid agar medium was essential for the consistent detection of Salmonella in the different lagoon effluents. Total and fecal coliform levels of up to 105-106 MPN/100 ml were detected in the final effluent. High coliphage concentrations of 103-104 pfu/ml were also found in the effluent waters. Salmonella was always detected in 100 ml samples and eventually reached an order of value of 103 MPN/100 ml. Total coliform reduction was higher in the anaerobic ponds whereas fecal coliforms were more efficiently eliminated in the facultative (mostly “anoxic”) lagoons. Coliphage reduction was higher in the facultative lagoons when compared to the anaerobic ponds. On many occasions, no reduction or eventual increment of the concentration of salmonellae was detected in the effluents from the anaerobic ponds compared to concentrations of the patohogen in the influent raw wasterwaters. The possibility exists for a capacity of Salmonella to multiply in the anoxic phase of the wastewater treatment, but the presence of microorganisms in raw sewage waters which could maskSalmonella detection with the enrichment methodology employed cannot be ruled out.


2018 ◽  
Vol 16 (12) ◽  
pp. 895-905 ◽  
Author(s):  
David A. Crook ◽  
Brendan J. Adair ◽  
Mark A. Grubert ◽  
Thor M. Saunders ◽  
John R. Morrongiello ◽  
...  

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