scholarly journals Bioremediation of artificially crude oil polluted soil of veritas University Abuja using poultry manure

2021 ◽  
Vol 6 (4) ◽  
pp. 498-507
Author(s):  
Ozioma L. Ugwu ◽  
Michael U. Orji ◽  
Odera R. Umeh ◽  
Benjamin Nma Yisa ◽  
Anthonia O. Oyegue
Keyword(s):  
Crude Oil ◽  
Natural Attenuation ◽  
Initial Moisture Content ◽  
Poultry Manure ◽  
Complete Removal ◽  
Polluted Soil ◽  
Bacillus Species ◽  
Isolation And Identification ◽  
Manure Treatment ◽  
Hydrocarbon Content

The removal of hydrocarbon compounds from the environment has always been a difficult undertaking for people all over the world. As a result, remedial efforts are required to safeguard the environment as well as to restore agriculture. This study looked at how poultry manure (dung) could help in the bioremediation of Veritas University's intentionally crude oil polluted soil. The physical properties of the soil and total petroleum hydrocarbon content of the crude oil polluted soil were determined, followed by the isolation and identification of microorganisms present in the soil and poultry manure before and after pollution with crude oil. These analyses were done according to standard operating procedures. Within a six-month timeframe, the natural attenuation and poultry manure amendment option for remediating the crude oil-polluted soil were monitored and the residual hydrocarbon content of the polluted soil after remediation evaluated. There was an overall decrease in pH level during the experimental units containing 4 g amendment, 2 g amendment and the untreated polluted soil in the order 8.4 to 7.1, 8.4 to 7.2 and 8.1 to 6.7, respectively. Temperature was within 27oC and 31oC, the treated soil that contained 4 g dung had an initial moisture content of 58%, and 35% after remediation. The hydrocarbon utilizing bacteria isolated were Pseudomonas species, Staphylococcus species, and Bacillus species, while the hydrocarbon utilizing fungi isolated were Aspergillus niger, Fusarium solani and Candida albicans. There was more significant microbial increase in the sample containing 4 g poultry manure treatment than 2 g poultry manure treatment and natural attenuation. Total hydrocarbon quantity significantly decreased after six months with the complete removal of C4, C7, C19 and C37 from the treated sample containing 4 g poultry manure. This indicated that the negative effects of crude oil on the environment can be mitigated by adding poultry manure.

scholarly journals Heavy Metal Tolerance Bacillus spp Isolated From Crude Oil Polluted Soil

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Muibat Fashola
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Crude Oil ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Polluted Soil ◽  
Diffusion Method ◽  
Bacillus Species ◽  
Antibiotics Resistance ◽  
Bacillus Spp

Introduction: Indiscriminate dumping of spent oils enriched with heavy metals has led to increase in heavy metals load in the soil. Heavy metals exert toxic effects on biodegradation of organic pollutant in cocontaminated soil and there is need to find suitable strategies for their removal. Aim: The aim of this study was to assess the heavy metals resistance capability of indigenous Bacillus species in hydrocarbon polluted soil to nickel (Ni), Cadmium (Cd), Lead (Pb) and Chromium (Cr). Materials and Methods: Heavy metal tolerant bacteria were isolated from hydrocarbon polluted soil using Luria-Berthani agar supplemented with the respective metals and spread plate techniques. The isolates were putatively identified on the basis of their colonial morphology and biochemical characteristics and their antibiotics susceptibility pattern were evaluated using disc diffusion method. Results: The maximum tolerable concentration (MTC) of the four heavy metals to the selected isolates was 2 mM. Four bacteria isolates able to withstand the MTC were putatively identified as Bacillus subtilis, Bacillus megaterium, Bacillus laterosporus and Bacillus polymyxa. Out of the four Bacillus species, only B. laterosporus did not show multiple tolerance to the tested antibiotics which show that there is correlation between heavy metal tolerance and antibiotics resistance by the isolates. Conclusion: Multiple heavy metal tolerance Bacillus spp. were isolated from crude oil polluted soil. These bacteria could be suitable agents for bioaugmentation of hydrocarbon polluted soil co-contaminated with heavy metals.

scholarly journals INDUCED DEGRADATION OF CRUDE OIL POLLUTED SOIL BY MICROBIAL AUGMENTATION

2017 ◽  
Vol 23 (4) ◽  
pp. 37-44
Author(s):  
SYLVESTER UWADIAE ◽  
EMIKE OMOAYENA
Keyword(s):  
Crude Oil ◽  
Soil Sample ◽  
Contaminated Soil ◽  
Polluted Soil ◽  
Soil Samples ◽  
The Other ◽  
Pseudomonas Sp ◽  
Hydrocarbon Content ◽  
Percentage Removal

This study was aimed at assessing the effectiveness of indigenous microbes for remediation of hydrocarbon contaminated soil by first increasing the population of the indigenous microbes via bioaugmentation. Soil samples were treated using a consortium of bacteria: Bacillus substilis and Pseudomonas sp. which were isolated and cultured from the contaminated soil. The non-bacteria injected soil sample had the highest hydrocarbon content (THC) of 271.021 in comparison with the other soil samples. The THC percentage removal of B1 (96.885 %), B2 (97.562 %), B3 (98.835 %), B4 (99.594 %) and B5 (99.540 %) were higher than that of the control; indicating that biodegradation actually took place.

scholarly journals INDUCED DEGRADATION OF CRUDE OIL POLLUTED SOIL BY MICROBIAL AUGMENTATION

2018 ◽  
Vol 23 (4) ◽  
Author(s):  
SYLVESTER UWADIAE ◽  
EMIKE OMOAYENA
Keyword(s):  
Crude Oil ◽  
Soil Sample ◽  
Contaminated Soil ◽  
Polluted Soil ◽  
Soil Samples ◽  
The Other ◽  
Pseudomonas Sp ◽  
Hydrocarbon Content ◽  
Percentage Removal

<p>This study was aimed at assessing the effectiveness of indigenous microbes for remediation of hydrocarbon contaminated soil by first increasing the population of the indigenous microbes via bioaugmentation. Soil samples were treated using a consortium of bacteria: <em>Bacillus substilis</em> and <em>Pseudomonas sp</em>. which were isolated and cultured from the contaminated soil. The non-bacteria injected soil sample had the highest hydrocarbon content (THC) of 271.021 in comparison with the other soil samples. The THC percentage removal of B1 (96.885 %), B2 (97.562 %), B3 (98.835 %), B4 (99.594 %) and B5    (99.540 %) were higher than that of the control; indicating that biodegradation actually took place.</p>

scholarly journals Stimulating the Rate of Phytoremediation of Chromolaena odorata in Crude Oil Contaminated Soil Using Inorganic Urea

2020 ◽  
pp. 13-25
Author(s):  
F. B. G. Tanee ◽  
K. Jude
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Latin Square ◽  
Polluted Soil ◽  
Total Petroleum Hydrocarbon ◽  
Test Plant ◽  
Chromolaena Odorata ◽  
Hydrocarbon Content ◽  
Total Hydrocarbon Content

Investigation on the use of urea in stimulating the phytoremediation of Chromolaena odorata in a crude oil contaminated soil was carried out at a crude oil spilled site at Botem-Tai, Ogoni, Nigeria. Three phytoremediation treatments labeled A – C in addition to the control (D) were used. The treatments were: A (Chromolaena odorata only), B (Chromolaena odorata + 20 g/m2 urea), C (Chromolaena odorata + 40 g/m2 urea), D (polluted soil without phytoremediation) arranged using Latin Square Design (LSD). Total petroleum hydrocarbon (TPH) and Total hydrocarbon content (THC) in soil and plant samples from the different treatment plots in addition to other soil nutrients were analyzed. The percentage reduction in TPH and THC in soil were as follows: Treatment B, {TPH (92.08%) and THC (95.37%)} > treatment A {TPH (88.95%) and THC (93.37%)}> C {TPH (78.78%) and THC (83.29%)} > Control {TPH (14.76%) and THC (32.90%)}. Treatment C had the highest TPH (2.67 mg/kg) and THC (20.57 mg/kg) accumulation in test   plant. Combining stimulant (urea) with phytoremediation also improved soil properties such  as pH, Nitrogen and Potassium. With the highest reduction of TPH and THC in treatment B (phytoremediation with 20 g/m2 urea) than other treatments is an indication that low   concentration of urea has a stimulatory effect on phytoremediation of crude oil by Chromolaena odorata.

scholarly journals Remediative Capacity of Crude Oil-polluted Soil After Exposure to Poultry Manure and Phosphate Minerals

1970 ◽  
Vol 23 (10) ◽  
pp. 1893-1900
Author(s):  
N.O Evbuomwan ◽  
F.E. Oviasogie ◽  
P.O. Oviasogie ◽  
B. Ikhajiagbe
Keyword(s):  
Crude Oil ◽  
Phosphate Rock ◽  
Poultry Manure ◽  
Nitrogen And Phosphorus ◽  
Total Hydrocarbon ◽  
Hydrocarbon Content ◽  
Phosphate Mineral ◽  
Two Factors ◽  
Free Environment ◽  
The Impact

This study investigated the impact of two soil amendments (poultry manure and phosphate rock minerals) on the intrinsic remediation capacity of a crude oil-contaminated soil. Well drained top soil (0-10 cm) was polluted with crude oil at the rate of 0.02mL/g of soil and amended with phosphate rock (PR) and poultry manure in three batches at 20 g, 30 g, 40 g and 50 g per 500 g of soil respectively in plastic bowls. Soil samples were collected from plastic bowls for physicochemical, microbiological, biodegradability and total hydrocarbon contents analyses. Results revealed improvement in physicochemical parameters in pH, total nitrogen and phosphorus of 7.00-7.50, 0.12-0.22 %, and 13.20- 65.42 mg/100 g after remediation against 4.30, 0.02 % and 6.05 mg/100 g recorded in day zero respectively. Bacillus subtilis (2.01), Pseudomonas aeruginosa (1.94), Mucor mucedo (2.47) and Penicillium notatum (2.43) had high biodegradation potential (NTU). The remediation efficiency of total hydrocarbon content after remediation was enhanced by two factors; increased concentrations of amendments of 50 g of poultry manure (4.62 mg/100 g; 66.45 %) and phosphate rock (0.33 mg/100 g; 97.60 %); And by application of combined amendments of both poultry manure and phosphate rock (0.19 mg/100 g; 98.62 %) compared to control (8.46mg/100 g; 38.56 %). Poultry manure and phosphate rock amendments enhance bioremediation efficiency in clean-up of crude oil polluted site and obviously a contamination free environment is a healthy and safe environment for all.Keywords: Bioremediation, Poultry manure, Phosphate mineral, Hydrocarbon content

scholarly journals Enhancement of the soil quality of an oil-polluted ultisol using livestock wastes

2020 ◽  
Vol 12 (2) ◽  
pp. 387-398
Author(s):  
Sylvia O. OGOANAH ◽  
Uzoamaka N. NGWOKE ◽  
Edokpolor O. OHANMU ◽  
Pascal C. OKOYE ◽  
Beckley IKHAJIAGBE
Keyword(s):  
Soil Quality ◽  
Polluted Soil ◽  
Lubricating Oil ◽  
Panicum Maximum ◽  
Potential Candidate ◽  
Bacterial Colony ◽  
Total Hydrocarbon ◽  
Hydrocarbon Content ◽  
Total Hydrocarbon Content

The study investigated the enhancement of soil quality of an oil-polluted ultisol using livestock wastes. Top soil (0 - 10 cm) was obtained as a pooled sample and polluted with spent lubricating oil at 10% w/w. The soil was subsequently amended with sun-dried goat (GT), rabbit (RB), and poultry (PG) dung at 10% w/w on dry weight basis both in singles, double-mixed, and triple-mixed combinations. Twelve weeks after treatment application, results showed that there was a 93.9% decrease (p<0.05) in bacterial colony count in the oil-polluted soil compared to the control. Penicillium notatum and Aspergillus niger as well as Bacillus sp. and Proteus sp. were the prominent fungal and bacterial species identified respectively. The most abundant plant in the soil seed bank was Panicum maximum with 10.4% abundance and this showed possible involvement of the plant in remediation of oil-pollution. The total hydrocarbon content of the oil-polluted soil was 9984.0 mg/kg, compared to 3170.6 mg/kg when amended with RB+GT, implying 76.77% remediation efficiency. Among several trials employed in this study, the combination of rabbit and goat wastes proved to be more effective in reducing the total hydrocarbon content of oil-polluted soil and therefore, is recommended as a potential candidate for application in the bioremediation of such soil.

scholarly journals Crude oil polluted soil remediation using poultry dung (chicken manure)

2019 ◽  
Vol 13 (10) ◽  
pp. 402-409
Author(s):  
Olusanya Emmanuel Oludele ◽  
Damilola Tope Ogundele ◽  
Kayode Odeniyi ◽  
Olayinka Shoyode
Keyword(s):  
Crude Oil ◽  
Soil Remediation ◽  
Polluted Soil ◽  
Chicken Manure
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