scholarly journals Translational Medicine and Autologous Fat Transfer: From bench to bedside. Regenerative and proliferative potential of adipose tissue

Author(s):  
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Aim: Autologous fat transfer (AFT) for the correction of maxillofacial defects was first reported at the end of the 19th century. AFT was introduced as a way of improving facial esthetics and in the last few years has expanded into applications in craniomaxillofacial reconstructive surgery and regenerative therapy. This protocol is part of translational medicine. The aim of translational medicine, or translational science, is to combine disciplines, resources, expertise, and techniques based on three main pillars: bench, bedside, and community. The primary goals to coalesce assets of various natures to significantly improve the global healthcare system. AFT is thus part of translational medicine in tissue healing, regeneration and augmentation. Methods: Several techniques have been suggested for harvesting and grafting the fat. The Authors follow Coleman’s technique with centrifugation and infiltration using different types and sizes of cannulas. Conclusion: This review provides a fairly comprehensive summary of the many exciting possibilities that exist in the field of facial fat grafting. Five clinical cases are presented and discussed. Translational medicine is the basis of this new reconstructive and regenerative field of science and research.

2016 ◽  
Vol 02 (02) ◽  
pp. e19-e30 ◽  
Author(s):  
Daniel Muench

Background The BEAULI -method (Breast Augmentation by Lipotransfer) is available for extraction and processing of large transplantable fat quantities. The aim of this work is to describe the surgical technique precisely and reproducibly and to provide an overview of the autologous fat transfer based on surgical experience. Method The author performed 300 autologous fat transplantations on 254 women between September 3, 2010, and May 13, 2015. Patients desiring moderate volume increase, fuller and firmer breasts, as well as an optimization of the silhouette, ideally with the concurrent desire of the correction of unwanted fat deposits, were selected. The fat was extracted via water-jet assisted liposuction (Body-jet, Human Med AG, Schwerin, Germany), and the fat cells were subsequently separated with the Lipocollector® (Human Med AG, Schwerin, Germany). Results The results were assessed with a control exam and photo comparison and were based on the responses on a questionnaire. Overall, 35.9% of the patients defined the result as very good, 38.6% as good, 22.4% as satisfactory, and 3.1% as poor. Conclusion This study shows that the autologous fat cell transplantation into the female breast via water-jet assisted liposuction achieves a moderate and harmoniously appearing breast volume enlargement as well as contour improvement. Further studies with more cases and longer observation periods over several years could contribute to improving the method of the autologous fat transfer regarding the grow-in rate, efficiency, and safety.


Author(s):  
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Aim: Frontal linear scleroderma, also known as ‘‘en coup de sabre,’’ is a congenital deformity characterized by atrophy and furrowing of the skin of the front parietal area above the level of the eyebrows. In most cases it occurs as a single paramedian line that may be associated with hypoplasia of underlying structures and facial hemiatrophy. In case of a wide lesion many reconstructive strategies have been proposed. The modern approach is aimed at augmentation of the tissue deficiency by using lipostructure and tissue regeneration. Autologous fat transfer (AFT) seems to be an effective strategy to restore the normal volume and contour of the face while providing a source of adipose-derived stem cells (ADSCs) with a multilineage differentiation potential. Methods: In this report, we present a rare case of linear scleroderma en coupe de sabre which was successfully managed with three stages of autologous fat grafting. Conclusion: The proposed uses for ADSCs in tissue repair and regeneration are quite impressive. Recent works on ADSCs would suggest that adult cells may prove to be an equally powerful regenerative tool in treating congenital and acquired maxillofacial disorders. More importantly, physicians, researchers and international associations need to cooperate in informing clinicians about what practices are based on evidence and to encourage support of additional research. There is increasing interest in a possible therapeutic effect of ADSCs from processed lipoaspirate for a wide spectrum of clinical applications in the facial and craniofacial area. AFT can be used in any facial area where soft tissue is lacking or where there is scarring, producing natural and long-lasting results. Mesenchymal stem cells represent a great tool in regenerative medicine. However, more definitive studies are needed to answer specific questions regarding the best technique to be used and the role of ADSCs. Autologous fat grafting provides a safe and easy approach for the treatment of linear scleroderma en coup de sabre, long-term clinically satisfactory results can be obtained.


2003 ◽  
Vol 20 (1) ◽  
pp. 17-25 ◽  
Author(s):  
John G. Rose ◽  
Bradley N. Lemke ◽  
Mark J. Lucarelli ◽  
Cynthia A. Boxrud ◽  
Kathryn L. Dortzbach ◽  
...  

Introduction: Autologous fat transfer is an increasingly popular treatment for the aging face. Recently, studies have suggested increased duration of effect with the injection of fat into or adjacent to muscles. Subcutaneous depths of facial muscles have been demonstrated using cadaver dissections, but to our knowledge this is the first study to use radiologic measurements in living subjects in order to quantify muscle depths in the face. Materials and Methods: Forty computed tomography (CT) scans were reviewed retrospectively using digital radio imaging software with a calibrated digital measurement tool that was used to measure the depths of the muscles of facial expression corresponding to fat-grafting sites. Correlation was made to formalin-preserved cadaver dissections. Results: Subcutaneous depths of facial muscles are listed as measured radio graphically and on cadaver sections. Discussion: The depths of the muscles of facial expression are more accurately measured using digital radiographs; these depths are important landmarks for facial cosmetic surgeons performing an autologous fat transfer. To our knowledge, this is the first study to characterize the anatomic depth of the facial muscles in the living human.


2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Marcos Sforza ◽  
Nicole Martinez ◽  
Nathalia Araujo ◽  
Roberto de Mezerville ◽  
Jose Andrés Castro

Abstract Autologous fat transfer is a common technique to refine the contour of the breast after prosthetic augmentation or reconstruction, correcting remaining asymmetries by injecting previously harvested fat tissue with a cannula. Current procedures are often performed without visualization of the cannula at the delivery site and may require subsequent verification of the implant’s integrity. The present paper aims to evaluate the safety of a new bulb tip cannula to be used during breast implant procedures for injecting fat adjacent to a breast implant that reduce the risk of damaging the implant. Two conventional cannulae and 3 bulb cannulae, which have an atraumatic distal tip, were tested in a simulated implant-puncture setting in 3 positions (at 0°, 45°, and 90° of incidence). A Tensile Tester (Instron, High Wycombe, UK) was used to apply force with each cannula device and record the amount of force applied in the attempt to penetrate the implants used, with shell layers having a variable thickness. No implant rupture was observed with the bulb tip cannulae regardless of size or the position in which the cannulae were pressed against the implants. The cannula opening was not impeded and tended to bend instead; 27% of the cases with the conventional lipo-cannulae caused an implant rupture. The bulb tip cannula could enhance the safety of the fat transfer procedure by ensuring no iatrogenic implant disruption and optimal delivery of the fat tissue.


2020 ◽  
pp. 074880682093856
Author(s):  
Alan Wong ◽  
Alexander D. J. Selsky ◽  
Alexander Zuriarrain

Gluteal fat grafting (or autologous fat transfer to the buttocks) is one of the fastest growing procedures in plastic surgery. However, as the popularity of the procedure has grown, so has the number of fatalities and complications. Fatalities are mainly linked to fat embolism, which can occur when fat is injected into the gluteal muscles, instead of subcutaneously. The use of wireless ultrasound imaging during the procedure can help reduce the chances of intramuscular fat injection and improve patient safety. Here, we present a 36-year-old female underwent circumferential liposuction with autologous fat transfer to the bilateral buttocks. From liposuction, 3500 mL of fat was harvested and 1000 mL of fat was injected into each buttock with wireless ultrasound guidance. We identified the subcutaneous plane where fat was to be injected, avoided penetrating the muscular fascia, and observed (in real-time) fat being introduced into the subcutaneous tissue. Wireless ultrasound-guided gluteal fat grafting is an effective and reliable method of visualizing the anatomy of the gluteal region and preventing intramuscular injection of fat. The technology is readily available, very user-friendly and serves as a great learning tool. Based on our positive experience using ultrasound guidance, larger comparative studies should be conducted to confirm its value.


2014 ◽  
Vol 3 (1) ◽  
pp. 62-69
Author(s):  
Kshemendra Senarath-Yapa ◽  
Rebecca Garza ◽  
Adrian McArdle ◽  
Graham Walmsley ◽  
Michael Hu ◽  
...  

2014 ◽  
pp. 431-448
Author(s):  
Paolo Persichetti ◽  
Barbara Cagli ◽  
Tiziano Pallara ◽  
Donata Vaccaro ◽  
Carlo Mallio ◽  
...  

2017 ◽  
Vol 4 (S) ◽  
pp. 100
Author(s):  
M.I. Muradov ◽  
K.B. Mukhamedkerim ◽  
A. ABaiguzeva ◽  
K.E. Kazantaev ◽  
D.Zh. Koshkarbaev

Background: To provide quantitative objective data demonstrating the longevity and amount of volume augmentation in the fatty dystrophy of the facial tissue obtained with autologous lipofilling.   Methods: In our clinic had been operated 8 patients for last 2 years with fatty dystrophy of the facial tissue. A prospective analysis of all patients who underwent at our private practice and were followed up for at least 1,5 year. Surgery was performed under general anesthesia it is necessary for clear results tissue correction. We based on the literature has seen numerous clinical reports highlighting the benefits of autologous fat transfer for face from that areas, fat was collected from the abdomen (most frequently used donor site), hips, outer thighs (saddle-bags), internal knee or thigh, with quantitative volume measurements evaluating the amount of postoperative volume change.   Results: Twenty eight patients were included in the study. The mean follow-up time was 18 months. The mean amount of autologous fat injected into each face region was 10-70 mL. Hypercorrection was performed after 3 months and it was 20-50% of the initial injected fat volume. Overall, the mean absolute volume augmentation measured at their last (after 6 month) post operative visit was 10-25%. There was variability between patients in the volume amount and percentage that remained. The resorption process was observed in two patients after 6 month. We made correction with hyaluronic acid and silicone implants.   Conclusion: To our knowledge, this study is the first clinical quantification in our practise of autologous fat transfer and/or grafting in the literature that provides definitive evidence on the amount as well as the resultant longevity in the face. Autologous fat transfer to the face has definite long-term volume augmentation results. On average, approximately 25-35% of the injected volume remains at 18 months. However, some variability exists in the percentage of  volume that remains that may require a touch-up procedure.


2021 ◽  
Vol 9 (8) ◽  
pp. e3734
Author(s):  
Shigeki Sakai ◽  
Naohiro Ishii ◽  
Yukie Nakamura ◽  
Kyoichi Matsuzaki ◽  
Shigemi Sakai ◽  
...  

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