scholarly journals Determination of Antibacterial Effect of Nannochloropsis oculata Against Some Rainbow Trout Pathogens

2021 ◽  
Vol 6 (2) ◽  
pp. 87-95
Author(s):  
I.tülay ÇAĞATAY ◽  
Mehmet ÖZBAŞ ◽  
H. Emre YILMAZ ◽  
Noha ALİ
Keyword(s):  
Rainbow Trout ◽  
Antibacterial Effect ◽  
Nannochloropsis Oculata

scholarly journals Advisability of using early generation Cephalosporines for prevention and treatment of postpartum infection

2017 ◽  
Vol 66 (3) ◽  
pp. 71-74
Author(s):  
Nikolay A. Korobkov
Keyword(s):  
Antibacterial Effect ◽  
Penetration Rate ◽  
Venous Blood ◽  
Uterine Cavity ◽  
Surgical Site Infections ◽  
Lime Treatment ◽  
Early Generation ◽  
E Coli ◽  
Highperformance Liquid Chromatography

Introduction: the following research investigates an availability of a long-lime treatment of postpartum infection by using of Cephalozolin (the Cephalosporine of generation I). Matherials and Methods. Lochia samples were obtained from the uterine cavities of 21 puerperal women who did not receive drug after delivery and were cultured for the identification and the determination of the susceptibilities of the clinical isolates to Cephalozolin. The concentrations of Cephalozolin in the supernatants were measured by highperformance liquid chromatography. Results. We determined the sensivity of the most clinically important bacteria to Cephazoline and its penetration rate to the uterine cavity. We found out the following bacteria which are sensible to Cephazoline – S. agalactiae, E. coli, K. pneumoniae, E. aerogenes. A low rate of Cephazoline ≤ 0,78 mg/ml was inhibited the growth of mentioned above bacteria. Cephazoline is also active as for S. aureus – MIC90 and was equal 3.13 mg/ml. In 3 hours after the injection of 1.0 gr of Cephazoline there was a maximum concentration in a venous blood – 1.63 mg/ml. The concentration of Cephazoline in lochia was rising gradually and it maximum in the uterus cavity in 5 hours was 1.26 mg/ml, and then its slowly went down. Conclusions. The mention above results let us suggest that Cephazoline is penetrates actively to lochia and its still has a good antibacterial effect. Cephazoline is still a treatment of choice as for prevention and a treatment of postpartum surgical site infections by a sensitive microorganism.

scholarly journals Determination of 3-Trifluoromethyl-4-Nitrophenol and 3-Trifluoromethyl-4-Nitrophenol Glucuronide in Edible Fillet Tissue of Rainbow Trout and Channel Catfish by Solid-Phase Extraction and Liquid Chromatography

2001 ◽  
Vol 84 (2) ◽  
pp. 392-398 ◽  
Author(s):  
Terrance D Hubert ◽  
Chue Vue ◽  
Jeff A Bernardy ◽  
Daniel L Van Horsen ◽  
Michelle I Rossulek
Keyword(s):  
Liquid Chromatography ◽  
Rainbow Trout ◽  
Solid Phase Extraction ◽  
Channel Catfish ◽  
Solid Phase ◽  
Phase Extraction ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Sea Lampreys ◽  
Uv Visible

Abstract 3-Trifluoromethyl-4-nitrophenol (TFM) is a pesticide used for the selective control of sea lampreys (Petromyzon marinus) in stream and river tributaries of the Great Lakes. To determine concentrations of TFM and TFM glucuronide in the edible fillet tissue of fish during sea lamprey control treatments, an analytical method was developed to determine the concentrations of these residues in rainbow trout (Oncorhynchus mykiss; RBT) and channel catfish (Ictalurus punctatis; CCF). Homogenized fillets were extracted with methanol–water (80 + 20). TFM and TFM glucuronide were isolated from coextractives by C18 solid-phase extraction. TFM glucuronide was hydrolyzed to TFM by the addition of β-glucuronidase to the TFM glucuronide extract. The extracts were analyzed separately by liquid chromatography with UV–visible detection. Recoveries from TFM-fortified CCF and RBT tissues were 84.1 and 96.1%, respectively. The method detection limits (MDLs) are 2.4 ng/g for TFM-fortified tissues of CCF and 3 ng/g for those of RBT. Recoveries were 78.8 and 77%from TFM glucuronide-fortified CCF and RBT tissues, respectively. The MDLs for TFM glucuronide-fortified tissues are 3.5 and 6.9 ng/g for CCF and RBT, respectively.

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