scholarly journals Escherichia coli O157:H7 Resistan Antibiotik Pada Daging Kebab yang Dijual di Sekitar Kampus IPB Dramaga Bogor

2021 ◽  
Vol 9 (3) ◽  
pp. 179-186
Author(s):  
Devi Yanti Sari ◽  
Herwin Pisestyani ◽  
Denny Widaya Lukman
Keyword(s):  
Escherichia Coli ◽  
Clinical Laboratory ◽  
Multidrug Resistant ◽  
Disk Diffusion ◽  
Escherichia Coli O157 ◽  
Foodborne Disease ◽  
E Coli ◽  
Diffusion Data

Kebab merupakan salah satu makanan siap saji atau ready to eat (RTE) yang populer di seluruh dunia. Escherichia coli (E. coli) O157:H7 banyak dihubungkan dengan kejadian outbreak foodborne disease pada kebab. Kontaminasi E. coli O157:H7 resistan antibiotik pada kebab dapat menimbulkan masalah kesehatan serius. Penelitian ini bertujuan mengidentifikasi E. coli O157:H7 resistan antibiotik yang diisolasi dari daging kebab yang dijual di sekitar Kampus IPB Dramaga Bogor. Total 43 sampel daging kebab diambil dari seluruh pedagang kebab di sekitar Kampus IPB Dramaga dalam radius 2 km dari batas terluar Kampus. Isolasi dan identifikasi E. coli mengacu pada Standar Nasional Indonesia (SNI) 2897:2008 dari Badan Standardisasi Nasional tentang Metode Pengujian Cemaran Mikroba dalam Daging, Telur, dan Susu, serta Hasil Olahannya. Uji serotyping E. coli O157:H7 menggunakan uji Serologis. Uji resistansi E. coli O157:H7 mengacu pada standar Clinical Laboratory Standards Institute (CLSI) dan dilakukan terhadap 10 jenis antibiotik menggunakan metode Kirby-Bauer disk diffusion. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan enam isolat positif E. coli O157:H7 (31.6%; 6/19) yang resistan terhadap ampisilin, amoksisilin-asam klavulanat, sefotaksim, gentamisin, siprofloksasin, enrofloksasin, kolistin sulfat dengan satu isolat termasuk multidrug resistant (MDR). Semua isolat E. coli O157:H7 masih sensitif terhadap trimethoprim-sulfametoksasol, oksitetrasiklin, dan kloramfenikol.

scholarly journals Isolation and Identification of Escherichia coli O157:H7 Lytic Bacteriophage from Environment Sewage

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Tessa Sjahriani ◽  
Eddy Bagus Wasito ◽  
Wiwiek Tyasningsih
Keyword(s):  
Escherichia Coli ◽  
Host Specificity ◽  
Pathogenic Bacteria ◽  
Statistical Tests ◽  
Plaque Morphology ◽  
Escherichia Coli O157 ◽  
Lytic Bacteriophage ◽  
Foodborne Disease ◽  
Isolation And Identification ◽  
E Coli

Escherichia coli O157:H7 is one of the pathogenic bacteria causing foodborne disease. The use of lytic bacteriophages can be a good solution to overcome the disease. This study is aimed at isolating lytic bacteriophages from environmental sewage with E. coli O157:H7 bacterial cells. The sample used in this study was eight bacteriophages, and the technique used in identifying E. coli O157:H7 carriers of the stx1 and stx2 genes was PCR. The double layer plaque technique was used to classify bacteriophages. Plaque morphology, host specificity, and electron micrograph were used to identify the bacteriophages. The result obtained plaque morphology as a clear zone with the largest diameter size of 3.5 mm. Lytic bacteriophage could infect E. coli O157:H7 at the highest titer of 10 × 10 8   PFU / mL . Bacteriophages have been identified as Siphoviridae and Myoviridae. Phage 3, phage 4, and phage 8 could infect Atypical Diarrheagenic E. coli 1 (aDEC1) due to their host specificity. The Friedman statistical tests indicate that lytic bacteriophage can significantly lyse E. coli O157:H7 ( p = 0.012 ). The lysis of E. coli O157:H7 by phage 1, phage 2, phage 3, and phage 5 bacteriophages was statistically significant, according to Conover’s posthoc test ( p < 0.05 ). The conclusion obtained from this study is that lytic bacteriophages from environmental sewage could lyse E. coli O157:H7. Therefore, it could be an alternative biocontrol agent against E. coli O157:H7 that contaminates food causing foodborne disease.

Efficacy of Chemical Interventions against Escherichia coli O157:H7 and Multidrug-Resistant and Antibiotic-Susceptible Salmonella on Inoculated Beef Trimmings

2012 ◽  
Vol 75 (11) ◽  
pp. 1960-1967 ◽  
Author(s):  
IFIGENIA GEORNARAS ◽  
HUA YANG ◽  
GALATIOS MOSCHONAS ◽  
MATTHEW C. NUNNELLY ◽  
KEITH E. BELK ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Chemical Treatment ◽  
Sodium Metasilicate ◽  
Multidrug Resistant ◽  
Antimicrobial Treatment ◽  
Sodium Chlorite ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Salmonella Newport

Studies were conducted to compare the decontamination efficacy of six chemical treatments against Escherichia coli O157:H7 and multidrug-resistant and antibiotic-susceptible Salmonella inoculated on beef trimmings. The inocula, comprising four-strain mixtures of rifampin-resistant E. coli O157:H7 and antibiotic-susceptible or multidrug-resistant (MDR and/or MDR-AmpC) Salmonella Newport and Salmonella Typhimurium, were inoculated (3 log CFU/cm2) separately onto samples (10 by 5 by 1 cm) derived from beef chuck rolls. Samples were left untreated (control), were immersed for 30 s in acidified sodium chlorite (0.1%, pH 2.5), peroxyacetic acid (0.02%, pH 3.8), sodium metasilicate (4%, pH 12.6), Bromitize Plus (0.0225% active bromine, pH 6.6), or AFTEC 3000 (pH 1.2), or were immersed for 5 s in SYNTRx 3300 (pH 1.0). Levels of surviving Salmonella on treated trimmings were not influenced by serotype or antibiotic resistance phenotype and were generally similar (P &gt; 0.05) or lower (P &lt; 0.05) than levels of surviving E. coli O157:H7 regardless of antimicrobial treatment. Overall, depending on chemical treatment (reductions within each chemical treatment were similar among all tested inocula), initial counts of E. coli O157:H7 (2.7 to 3.1 log CFU/cm2) were reduced (P &lt; 0.05) by 0.2 to 1.4 log CFU/cm2. Similarly, initial counts of the tested Salmonella inocula (2.8 to 3.3 log CFU/cm2) were reduced (P &lt; 0.05) by 0.4 to 1.4 (Salmonella Newport, antibiotic susceptible), 0.3 to 1.4 (Salmonella Newport, MDRAmpC), 0.2 to 1.5 (Salmonella Typhimurium, antibiotic susceptible), 0.4 to 1.3 (Salmonella Typhimurium, MDR), and 0.4 to 1.5 (Salmonella Typhimurium, MDR-AmpC) log CFU/cm2, depending on antimicrobial treatment. Reductions obtained with sodium metasilicate were 1.3 to 1.5 log CFU/cm2, regardless of inoculum, and reductions obtained with the five remaining antimicrobial treatments were 0.2 to 0.7 log CFU/cm2 (depending on treatment). Findings of this study should be useful to regulatory authorities and the meat industry as they consider Salmonella contamination on beef trimmings.

Prevalence of Salmonella Serovars, Listeria monocytogenes, and Escherichia coli O157:H7 in Mediterranean Ready-to-Eat Meat Products in Jordan

2014 ◽  
Vol 77 (1) ◽  
pp. 106-111 ◽  
Author(s):  
TAREQ M. OSAILI ◽  
ANAS A. AL-NABULSI ◽  
REYAD R. SHAKER ◽  
ZIAD W. JARADAT ◽  
MOHAMMAD TAHA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Meat Products ◽  
Multidrug Resistant ◽  
Automated System ◽  
Escherichia Coli O157 ◽  
Food Control ◽  
E Coli ◽  
Beef Products ◽  
Salmonella Serovars

The presence of Salmonella, Listeria monocytogenes, and Escherichia coli O157:H7 in ready-to-eat (RTE) meat products is considered a major concern for food control authorities worldwide. The aims of this study were to determine (i) the prevalence of Salmonella, L. monocytogenes, and E. coli O157:H7 in Mediterranean RTE chicken and beef (CB) products sold in Jordanian restaurants and (ii) the susceptibility of the isolates to antibiotics. A total of 1,028 samples of various types of RTE CB products (550 RTE chicken and 478 RTE beef products) were analyzed by methods described by the International Organization for Standardization followed by molecular confirmation of the isolates. The VITEK2 automated system was used for testing antibiotic susceptibility of the isolates. The overall prevalence of Salmonella serovars in RTE CB products was 0.5%, with 0.8 and 0.2% in RTE chicken and RTE beef, respectively. The overall prevalence of L. monocytogenes in RTE CB products was 2%, with 2.7 and 1.5% in RTE chicken and RTE beef products, respectively. E. coli O157:H7 was not isolated from any of the tested samples. Multidrug-resistant Salmonella and L. monocytogenes isolates were found. The majority of Salmonella isolates were sensitive to most of the tested antibiotics, and all of the isolates were resistant to more than one antibiotic. Similarly, more than 85% of L. monocytogenes isolates were sensitive to nine antibiotics, and the majority of L. monocytogenes isolates were resistant to fosfomycin and oxacillin.

scholarly journals Molecular Epidemiology of Ceftiofur-Resistant Escherichia coli Isolates from Dairy Calves

2006 ◽  
Vol 72 (6) ◽  
pp. 3940-3948 ◽  
Author(s):  
Sarah C. Donaldson ◽  
Beth A. Straley ◽  
Narasimha V. Hegde ◽  
Ashish A. Sawant ◽  
Chitrita DebRoy ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Dairy Herd ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Dairy Calves ◽  
Disk Diffusion ◽  
Pulsed Field ◽  
E Coli ◽  
Clonal Type

ABSTRACT Healthy calves (n = 96, 1 to 9 weeks old) from a dairy herd in central Pennsylvania were examined each month over a five-month period for fecal shedding of ceftiofur-resistant gram-negative bacteria. Ceftiofur-resistant Escherichia coli isolates (n = 122) were characterized by antimicrobial resistance (disk diffusion and MIC), serotype, pulsed-field gel electrophoresis subtypes, beta-lactamase genes, and virulence genes. Antibiotic disk diffusion assays showed that the isolates were resistant to ampicillin (100%), ceftiofur (100%), chloramphenicol (94%), florfenicol (93%), gentamicin (89%), spectinomycin (72%), tetracycline (98%), ticarcillin (99%), and ticarcillin-clavulanic acid (99%). All isolates were multidrug resistant and displayed elevated MICs. The E. coli isolates belonged to 42 serotypes, of which O8:H25 was the predominant serotype (49.2%). Pulsed-field gel electrophoresis classified the E. coli isolates into 27 profiles. Cluster analysis showed that 77 isolates (63.1%) belonged to one unique group. The prevalence of pathogenic E. coli was low (8%). A total of 117 ceftiofur-resistant E. coli isolates (96%) possessed the bla CMY2 gene. Based on phenotypic and genotypic characterization, the ceftiofur-resistant E. coli isolates belonged to 59 clonal types. There was no significant relationship between calf age and clonal type. The findings of this study revealed that healthy dairy calves were rapidly colonized by antibiotic-resistant strains of E. coli shortly after birth. The high prevalence of multidrug-resistant nonpathogenic E. coli in calves could be a significant source of resistance genes to other bacteria that share the same environment.

scholarly journals First Isolation and Molecular Characterization of blaCTX-M-121-Producing Escherichia coli O157:H7 From Cattle in Xinjiang, China

2021 ◽  
Vol 8 ◽  
Author(s):  
Zhanqiang Su ◽  
Panpan Tong ◽  
Ling Zhang ◽  
Mengmeng Zhang ◽  
Dong Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogen ◽  
Virulence Gene ◽  
Multidrug Resistant ◽  
Escherichia Coli O157 ◽  
Bacterial Strains ◽  
E Coli ◽  
Uremic Syndrome ◽  
Cattle Farms

The bovine Escherichia coli O157:H7 is a major foodborne pathogen causing severe bloody diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome in humans. Cattle are recognized major reservoir and source of E. coli O157:H7. We investigated the antibiotic resistance, molecular profiles, and intrinsic relationship between 21 isolates of E. coli O157:H7 from cattle farms and slaughtering houses in Xinjiang. Using pulsed-field gel electrophoresis (PFGE) molecular typing, two types of PFGE were revealed through cluster analysis, including clusters I and II, with 66 and 100% similarity of PFGE spectra between 21 isolates. We also detected that 18 isolates (86%) carried at least one virulence gene, 16 isolates (76%) carried the eae gene, and 7 (33%) carried the stx1 + stx2 + eae + hly + tccp genes. Eighteen isolates were susceptible to antibiotics. Three isolates were resistant to antibiotics, and two were multidrug resistant. One of the two multidrug-resistant isolates detectably carried the blaCTX−M−121 gene. This is the first finding of the blaCTX−M−121 gene detected in E. coli O157:H7 isolated from cattle in Xinjiang. The blaCTX−M−121 gene is transferable between the bacterial strains via plasmid transmission. The results indicated that E. coli O157:H7 may have undergone clonal propagation in cattle population and cross-regional transmission in Xinjiang, China.

scholarly journals Isolation and Characterization of Escherichia coli Sequence Type 131 and Other Antimicrobial-Resistant Gram-Negative Bacilli from Clinical Stool Samples from Veterans

2016 ◽  
Vol 60 (8) ◽  
pp. 4638-4645 ◽  
Author(s):  
Muhanad Mohamed ◽  
Connie Clabots ◽  
Stephen B. Porter ◽  
Paul Thuras ◽  
James R. Johnson
Keyword(s):  
Escherichia Coli ◽  
Clinical Laboratory ◽  
Medical Center ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Gram Negative ◽  
Content Type ◽  
E Coli ◽  
Isolation And Characterization ◽  
Stool Samples

ABSTRACTEmerging multidrug-resistant (MDR) Gram-negative bacilli (GNB), includingEscherichia colisequence type 131 (ST131) and its resistance-associatedH30 subclone, constitute an ever-growing public health threat. Their reservoirs and transmission pathways are incompletely defined. To assess diarrheal stools as a potential reservoir for ST131-H30 and other MDR GNB, we cultured 100 clinical stool samples from a Veterans Affairs Medical Center clinical laboratory (October to December 2011) for fluoroquinolone- and extended-spectrum cephalosporin (ESC)-resistantE. coliand other GNB, plus totalE. coli. We then characterized selected resistant and susceptibleE. coliisolates by clonal group, phylogenetic group, virulence genotype, and pulsotype and screened all isolates for antimicrobial resistance. Overall, 79 of 100 stool samples yielded GNB (52E. coli; 48 other GNB). Fifteen samples yielded fluoroquinolone-resistantE. coli(10 were ST131, of which 9 wereH30), 6 yielded ESC-resistantE. coli(2 were ST131, both non-H30), and 31 yielded susceptibleE. coli(1 was ST131, non-H30), for 13 total ST131-positive samples. Fourteen non-E. coliGNB were ESC resistant, and three were fluoroquinolone resistant. Regardless of species, almost half (46%) of the fluoroquinolone-resistant and/or ESC-resistant non-E. coliGNB were resistant to at least three drug classes. Fecal ST131 isolates closely resembled reference clinical ST131 isolates according to virulence genotypes and pulsed-field gel electrophoresis (PFGE) profiles. Thus, a substantial minority (30%) of veterans with diarrhea who undergo stool testing excrete antibiotic-resistant GNB, includingE. coliST131. Consequently, diarrhea may pose transmission risks for more than just diarrheal pathogens and may help disseminate clinically relevant ST131 strains and other MDR GNB within hospitals and the community.

Evaluation of Potential for Inhibition of Growth of Escherichia coli O157:H7 and Multidrug-Resistant Salmonella Serovars in Raw Beef by Addition of a Presumptive Lactobacillus sakei Ground Beef Isolate

2009 ◽  
Vol 72 (2) ◽  
pp. 251-259 ◽  
Author(s):  
JOHN R. RUBY ◽  
STEVEN C. INGHAM
Keyword(s):  
Escherichia Coli ◽  
Ground Beef ◽  
Multidrug Resistant ◽  
Significant Inhibition ◽  
Escherichia Coli O157 ◽  
Lactobacillus Sakei ◽  
E Coli ◽  
Inhibition Of Growth ◽  
Salmonella Serovars

The efficacy of adding presumptive Lactobacillus sakei (LS) strain 10-EGR-a, the most inhibitory from among 12 ground beef Lactobacillus isolates, to inhibit growth by Escherichia coli O157:H7 and multidrug-resistant (MDR) Salmonella (serovars Newport and Typhimurium) was evaluated in a beef-derived broth medium at 10°C and in fresh raw ground beef at 10 and 5°C. Pathogen inhibition was observed in the broth medium at both high (108:105 to 107:105) and low (106:105 to 105:105) LS:pathogen ratios. After 9 days at 10°C, in broth medium with high LS:pathogen ratios, growth of E. coli O157:H7 and MDR Salmonella was inhibited by an average of 2.6 and 3.2 log CFU/ml, respectively, whereas in broth medium with low LS:pathogen ratios, E. coli O157:H7 and MDR Salmonella growth was inhibited by an average of 2.8 and 1.8 log CFU/ml, respectively. However, in raw ground beef no significant inhibition was seen with LS:pathogen ratios of 105:102 to 105:103. Significant inhibition was seen at very high LS:pathogen ratios (106 to 107:102 to 103), but gross spoilage of the product occurred by day 6. Although presumptive LS 10-EGR-a can inhibit growth of E. coli O157:H7 and MDR Salmonella in a beef-derived broth medium, the inability to produce similar results in ground beef without deleteriously affecting the quality of the product is a limitation that needs further investigation.

Sensitivity of Shiga Toxin–Producing Escherichia coli, Multidrug-Resistant Salmonella, and Antibiotic-Susceptible Salmonella to Lactic Acid on Inoculated Beef Trimmings

2012 ◽  
Vol 75 (10) ◽  
pp. 1751-1758 ◽  
Author(s):  
ALIYAR FOULADKHAH ◽  
IFIGENIA GEORNARAS ◽  
HUA YANG ◽  
KEITH E. BELK ◽  
KENDRA K. NIGHTINGALE ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Salmonella Typhimurium ◽  
Shiga Toxin ◽  
Multidrug Resistant ◽  
Escherichia Coli O157 ◽  
Acid Solutions ◽  
E Coli ◽  
Salmonella Newport ◽  
Acid Treatments

Studies were performed to determine whether lactic acid treatments used to reduce Escherichia coli O157:H7 on beef trimmings are also effective in controlling non-O157 Shiga toxin–producing E. coli (nSTEC), and multidrug-resistant and antibiotic-susceptible Salmonella. Beef trimming pieces (10 by 5 by 1 cm) were inoculated (3 log CFU/cm2) separately with four-strain mixtures of rifampin-resistant E. coli O157:H7, O26, O45, O103, O111, O121, and O145. Similarly, in a second study, trimmings were separately inoculated with rifampin-resistant E. coli O157:H7, and antibiotic-susceptible or multidrug-resistant (MDR and/or MDR-AmpC) Salmonella Newport and Salmonella Typhimurium. Inoculated trimmings were left untreated (control) or were immersed for 30 s in 5% lactic acid solutions (25 or 55°C). No differences (P ≥ 0.05) were obtained among surviving counts of E. coli O157:H7 and those of the tested nSTEC serogroups on lactic acid–treated (25 or 55°C) samples. Counts (3.1 to 3.3 log CFU/cm2) of E. coli O157:H7 and nSTEC were reduced (P &lt; 0.05) by 0.5 to 0.9 (25°C lactic acid) and 1.0 to 1.4 (55°C lactic acid) log CFU/cm2. Surviving counts of Salmonella on treated trimmings were not influenced by serotype or antibiotic resistance phenotype and were similar (P ≥ 0.05) or lower (P &lt; 0.05) than surviving counts of E. coli O157:H7. Counts (3.0 to 3.3 log CFU/cm2) were reduced (P &lt; 0.05) by 0.5 to 0.8 (E. coli O157:H7) and 1.3 to 1.5 (Salmonella) log CFU/cm2 after treatment of samples with 25°C lactic acid. Corresponding reductions following treatment with lactic acid at 55°C were 1.2 to 1.5 (E. coli O157:H7) and 1.6 to 1.9 (Salmonella) log CFU/cm2. Overall, the results indicated that lactic acid treatments used against E. coli O157:H7 on beef trimmings should be similarly or more effective against the six nSTEC serogroups and against multidrug-resistant and antibiotic-susceptible Salmonella Newport and Salmonella Typhimurium.

Prevalence and Antimicrobial Resistance Profiles of Escherichia coli O157:H7 and Salmonella Isolated from Feedlot Lambs†

2009 ◽  
Vol 72 (8) ◽  
pp. 1713-1717 ◽  
Author(s):  
TOM S. EDRINGTON ◽  
MELISSA LONG ◽  
TIM T. ROSS ◽  
JACK D. THOMAS ◽  
TODD R. CALLAWAY ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Fecal Coliforms ◽  
Processing Plant ◽  
Escherichia Coli O157 ◽  
Fecal Samples ◽  
E Coli ◽  
Feedlot Lambs ◽  
Plant Processing ◽  
Carcass Contamination

The present study examined the incidence of Escherichia coli O157:H7 and Salmonella in feedlot lambs. Fifty-six feedlot lambs from eight sheep farming operations were grouped in a single drylot pen, fed, and managed as is typical in the southwestern United States. Fecal samples were collected on days 0, 46, 87, and 122 of the feeding period via rectal palpation. Wool samples (ventral midline) were collected one time only at the feedlot, immediately prior to shipping to the processing plant, and carcass swabs were collected following slaughter. All samples were cultured for E. coli O157:H7, Salmonella, and fecal coliforms, and select isolates were examined for antimicrobial susceptibility. Overall, the percentages of fecal and wool samples positive for E. coli O157:H7 averaged 9 and 18%, respectively. One carcass swab was E. coli O157:H7 positive. Of the 155 fecal samples collected, 11 (7%) were Salmonella positive. Salmonella was detected in nearly 50% of the wool samples collected prior to slaughter, while none of the carcasses were Salmonella positive 24 h postslaughter. All isolates (E. coli O157:H7, Salmonella, and fecal coliforms) were susceptible to ceftiofur, enrofloxacin, and trimethoprim-sulfamethoxazole. One E. coli O157:H7 isolate cultured from a carcass swab was resistant to seven antibiotics, and seven wool E. coli O157: H7 isolates were multidrug resistant. Results of this research demonstrate that feedlot sheep are naturally colonized with E. coli O157:H7 and Salmonella and wool can be a source of carcass contamination; however, in-plant processing procedures and intervention strategies were largely effective in preventing carcass contamination.

Comparison of Effects of Antimicrobial Interventions on Multidrug-Resistant Salmonella, Susceptible Salmonella, and Escherichia coli O157:H7†

2008 ◽  
Vol 71 (11) ◽  
pp. 2177-2181 ◽  
Author(s):  
TERRANCE M. ARTHUR ◽  
NORASAK KALCHAYANAND ◽  
JOSEPH M. BOSILEVAC ◽  
DAYNA M. BRICHTA-HARHAY ◽  
STEVEN D. SHACKELFORD ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Disease ◽  
Foodborne Pathogen ◽  
Multidrug Resistant ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Beef Processing ◽  
Processing Plants ◽  
Human Illness ◽  
Antimicrobial Interventions

Several strains of Salmonella have been identified as resistant to multiple antibiotics. What is not known is whether strains possessing multidrug resistance properties also have the ability to resist the killing effects of the antimicrobial interventions used in beef processing. The research project described herein was designed to determine whether antimicrobial interventions currently in place in beef processing facilities are adequate for reducing the foodborne pathogen loads on beef carcass surfaces contaminated with multidrug-resistant (MDR) Salmonella. The data presented here indicate that MDR Salmonella is reduced at least as effectively as are Escherichia coli O157:H7 and susceptible Salmonella when treated with antimicrobial interventions currently in use at most U.S. beef processing plants. The E. coli O157:H7 strains used in this study were divided into two groups, strains that have a genetic polymorphism associated with human disease and strains not typically found to cause human disease. No differences were detected in the abilities of these two strain types to survive antimicrobial interventions. These results indicate that neither the drug resistance status of a particular Salmonella strain nor the likelihood that a particular E. coli O157:H7 strain will cause human illness influences the antimicrobial efficacy of the interventions utilized by the modern beef processing plants.

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