scholarly journals Formulasi Whey Fermentasi dengan Penambahan Buah Merah (Pandanus conoideus Lam) sebagai Tabir Surya

2022 ◽  
Vol 10 (1) ◽  
pp. 28-33
Author(s):  
M. Ranggawati ◽  
I. I. Arief ◽  
Z. Wulandari
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Streptococcus Thermophilus ◽  
Lactobacillus Bulgaricus ◽  
Coarse Particles ◽  
Active Ingredients ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Fruit Oil ◽  
Sunscreen Formulation

Whey produced from cheese processing can be used as non-food products such as sunscreen by fermenting using Streptococcus thermophilus and Lactobacillus bulgaricus. Adding active ingredients such as red fruit oil which contains carotenoids can increase the effectiveness of sunscreen. This study aims to determine the formulation of fermented whey and red fruit oil usage in sunscreen. The study began with the production of fermented whey and sunscreen formulations with ratios of 0, 1:1, 1:2, and 2:1 (fermented whey: red fruit oil). Furthermore, observe the organoleptic, antioxidant, SPF, pH, and homogeneity value. Adding fermented whey and red fruit oil showed no significant effect (P>0.05) onthe organoleptic test of aroma, but significantly (P<0.05) on color and texture. The antioxidant activity value test showed significantly different results (P<0.05) with IC50 values classified as moderate to high.Determination of the SPF value of sunscreen showed significantly different results  (P<0.05), but this value was classified as low protection. Although there was no significant effect on pH testing (P>0.05), the pH of sunscreen cream indicated that it was suitable for topical application. All samples showed homogeneous results and there were no coarse particles or separation between the oil and water phases. The best sunscreen formulation is 1:1 cream with the highest SPF value of 6.86 and strong antioxidant activity with an IC50 value of 56.15 g/mL. Fermented whey and red fruit oil usage as active ingredients are more effective in increasing the protection of the cream against UV rays and free radicals.

scholarly journals Aktivitas Antioksidan Ekstrak Metanol Daun Alpukat Menggunakan Metode DPPH

2021 ◽  
Vol 11 (2) ◽  
pp. 104-110
Author(s):  
Alfin Surya ◽  
Zaiyar Nazir ◽  
Anggun Syazulfa
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radicals ◽  
Methanol Extract ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Phytochemical Screening ◽  
Bioactive Components ◽  
Ic50 Value ◽  
Ic50 Values

Free radicals are molecules that lose electrons in their outer orbitals so that the number of electrons becomes odd and unstable. Free radicals can damage various macromolecule cells including proteins, carbohydrates, fats. Antioxidants are compounds that can donate one or more electrons to free radicals. Avocado leaves contain high bioactive components that can be used as a source of natural antioxidants. Avocado leaves that have been dried, mashed and then shifted with methanol. The extracts were tested by phytochemical screening, including flavonpoid, phenolic, tannin and antioxidant activity using the DPPH (2,2-diphenyl-1-picrylhidrazyl) method. This method is based on the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) against free radicals which causes a change from purple to yellow 2,2-diphenyl-1-picrylhydrazyl) (DPPH). From the research, the IC50 values ​​in avocado leaves were 118.8056 µg / mL and 7,276 µg / mL on ascorbic acid as a positive control. From the IC50 value obtained, it is known that the methanol extract of avocado leaves has strong antioxidant activity to fight free radicals.

scholarly journals Antioxidant and Anti-Hyaluronidase Activities of Dragon Fruit Peel Extract and Kaempferol-3-O-Rutinoside

2019 ◽  
Vol 30 (4) ◽  
pp. 247
Author(s):  
Liana Liana ◽  
Rizal Rizal ◽  
Wahyu Widowati ◽  
Fioni Fioni ◽  
Khainir Akbar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Human Life ◽  
Fruit Peel ◽  
Paired Samples ◽  
Dragon Fruit ◽  
Ic50 Value ◽  
Significant Difference ◽  
Ic50 Values ◽  
Peel Extract

Aging is a natural process in human life and is triggered by the presence of free radicals (ROS). The use of antioxidants from natural ingredients is one of the breakthroughs to overcome aging and counteract the harmful effects caused by the free radicals. This study aimed to determine and compare the antioxidant activity of H2O2 scavenging and hyaluronidase inhibition of red dragon fruit peel extract (DFPE) and kaempferol-3-o-rutinoside (KOR) compounds. Dragon fruit peel extract (DFPE) is obtained through extraction by maceration method using 70% ethanol solvent. The design of this study included antioxidant and anti-aging activity assay of EKBN and KOR at the series concentration of 15.63; 31.25; 62.50; 125; 250; 500 µg/mL through H2O2 scavenging, as well as the DFPE and KOR hyaluronidase inhibition assay at the series concentration of 5.21; 10.42; 20.83; 41.7; 83.33; 166.67 µg/mL. EKBN shows that the average activity of H2O2 scavenging is lower than KOR. In addition, the IC50 values of KOR for H2O2 scavenging is lower (351.46±2.30ug/mL) than DFPE (409.64±23.17ug/mL). While, KOR also has higher values of inhibitory activity than of the DFPE. However, the IC50 value of KOR for hyaluronidase inhibition activity was 84.07±10.46µg/mL, equivalent to the IC50 value of DFPE (85.32±10.24µg/mL). The presence of antioxidant and anti-aging activity in the EKBN is probably caused by betalain and the KOR compound itself contained in red dragon fruit. The results of the paired-samples T-test on antioxidant activity and anti-aging of DFPE and KOR showed non-significant difference. Thus, DFPE has an equivalent antioxidant and anti-aging through H2O2 scavenging and hyaluronidase activity as possessed by the KOR compound.

scholarly journals SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOLIK BUAH BUNI (Antidesma bunius (L.) SPRENG)

2016 ◽  
Vol 3 (2) ◽  
pp. 159-163
Author(s):  
Arif Rahman ◽  
Abd Malik ◽  
Aktsar Roskiana Ahmad
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Free Radical Scavengers ◽  
Bioactive Components ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Dried Fruit ◽  
Ethanol Extracts

Free radicals play a role in the occurrence of various degenerative diseases that require free-radical scavengers or antioxidants. Buni fruit (Antidesma bunius (L.) Spreng) has the bioactive components are Anthocyanins (flavonoids) that serves to the free radicals. This study aimed to measure the antioxidant activity of theethanol extract contained 70% fruit Buni obtained by using the method of nitric oxide. Simplicia buni macerated dried fruit with 70% ethanol. Extracts were obtained in the test antioxidant activity against nitric oxide radicals. The antioxidant activity against free radical absorbance measured by means of UV-Vis spectrophotometry at a wavelength of 546 nm and calcul ated IC50 values. The results shows that the fruit buni has potential as a free radical with IC50 value of 2.28 µg/mL and a comparison of quercetin with IC50 value of 5.88 µg/mL.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK BAWANG HITAM (BLACK GARLIC) DENGAN VARIASI LAMA PEMANASAN

2020 ◽  
Vol 13 (1) ◽  
pp. 39-50
Author(s):  
Eva Agustina ◽  
Funsu Andiarna ◽  
Irul Hidayati
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Heating Time ◽  
The Body ◽  
Garlic Extract ◽  
Active Compounds ◽  
Rotary Evaporator ◽  
Ic50 Value ◽  
Ic50 Values ◽  
The Difference

AbstrakSenyawa radikal dalam tubuh sangat reaktif terhadap sel-sel, sehingga dapat menyebabkan berbagai penyakit. Senyawa yang mampu menangkal radikal bebas disebut antioksidan. Bawang hitam diketahui mengandung senyawa aktif fenolik dan turunannya sehingga dapat digunakan sebagai antioksidan. Penelitian tentang penggunaan bawang hitam sebagai antioksidan ini bertujuan untuk mengetahui perbedaan waktu pemanasan (15, 25, dan 35 hari) terhadap aktivitas antioksidan pada ekstrak bawang hitam. Bawang hitam diperoleh dengan cara memanaskan bawang putih selama 15, 25, dan 35 hari. Bawang hitam selanjutnya diekstraksi dengan pelarut metanol dengan metode maserasi. Filtrat hasil ekstraksi dipekatkan dengan rotary evaporator untuk mendapatkan ekstrak bawang hitam. Ekstrak bawang hitam diuji fitokimia untuk mengetahui kandungan senyawa aktif. Aktivitas antioksidan ekstrak bawang hitam ditentukan dengan metode penangkalan radikal 2,2-diphenyl-1-picrylhydrazyl (DPPH). Hasilnya menunjukkan ekstrak bawang hitam memiliki aktivitas antioksidan yang sangat kuat dengan nilai IC50 masing-masing 15 hari yaitu 2,41 µg/mL; 25 hari yaitu 2,93 µg/mL; 35 hari yaitu 2,27 µg/mL. Nilai IC50 <10 μg/mL menunjukkan bahwa ekstrak bawang hitam dengan waktu pemanasan 15, 25, dan 35 hari memiliki potensi antioksidan yang sangat kuat. Waktu pemanasan yang paling optimum dalam penangkalan radikal bebas adalah ekstrak bawang hitam dalam waktu pemanasan 35 hari, karena banyak terdapat kandungan senyawa flavonoid, tanin, sterol, dan saponin.Abstract Radical compounds in the body are very reactive to cells that can cause various diseases. Compounds that are able to ward off free radicals are called antioxidants. Black garlic are known to contain phenolic active compounds and their derivatives so that they can be used as antioxidants. Research on the use of black garlic as an antioxidant aims to determine the difference in heating time (15, 25, and 35 days) on the antioxidant activity of black garlic extract. Black garlic are obtained by heating the garlic for 15, 25, and 35 days. The black garlic is then extracted with methanol solvent by maceration method. The extracted filtrate was concentrated with a rotary evaporator to obtain the black garlic extract. Black garlic extract was tested for phytochemistry to determine the content of active compounds. The antioxidant activity of black garlic extract was determined by the radical deterrence method 2,2-diphenyl-1-picrylhydrazyl (DPPH). The results showed that the onion extract had a very strong antioxidant activity with an IC50 value of 15 days each of 2.41 µg/mL; 25 days which is 2.93 μg/mL; 35 days which is 2.27 µg/mL. IC50 values <10 μg/mL indicate that the black garlic extract with a heating time of 15, 25, and 35 days has a very strong antioxidant potential. The most optimum heating time in deterring free radicals is black garlic extract within 35 days of heating because there are many compounds containing flavonoids, tannins, sterols, and saponins.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Etanol Batang Daun dan Bunga Jumpai (Glinus oppositifolius (L.) Aug. DC.)

2021 ◽  
Vol 6 (1) ◽  
pp. 14
Author(s):  
Astuti Amin ◽  
Andi Paluseri ◽  
Rahmat Priyandi Linggotu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Antioxidant Potential ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Stem Leaf

 Jumpai (Glinus Oppoitifolius (L.) Aug. DC) contains flavonoids compounds that can act as antioxidants by donating hydrogen so as to stabilize the lack of electrons in free radicals. This study aims to determine the antioxidant potential of stem, leaf, and flower extracts by looking at the IC50 value. The stems, leaves, and flowers were extracted by maceration using 70% l ethanol solvent. The results of the antioxidant activity test using the DPPH method (2,2-diphenyl-1-picrilhydrazil) showed very strong antioxidant activity with IC50 values of 9.523 µg / ml stem, 32.89 µg / ml leaves, and 23.07 µg / ml flowers with vitamin comparisons. C obtained IC50 value of 1,698 µg / ml. Based on these results, it can be rejected that the stems, leaves, and flowers have antioxidant activity with a very strong category against DPPH free radicals (2,2-diphenyl-1-picrilhydrazil). 

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Irfan Zamzani ◽  
Nita Triadisti
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Oxidation Process ◽  
Antioxidant Activities ◽  
Alternative Source ◽  
Dpph Method ◽  
Indigenous Plant ◽  
Ic50 Values

Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Daun Ganitri (Elaeocarpus Ganitrus Roxb.) dengan Metode DPPH (2,2 Difenil-1-Pikrilhidazil)

2021 ◽  
Vol 18 (1) ◽  
pp. 60-67
Author(s):  
Naelaz Zukhruf Wakhidatul Kiromah ◽  
Sadam Husein ◽  
Titi Pudji Rahayu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Synthetic Antioxidant ◽  
Activity Test ◽  
Impaired Liver ◽  
Ic50 Value ◽  
Dpph Method

Free radicals are one of the cause of various diseases. The use of synthetic antioxidant compounds could prevent the effect of the free radicals, however may cause adverse effects on the human body such as impaired liver, lung, intestinal and poisoning. Therefore antioxidant from natural resources needs to be developed. The purpose of this research was to determine the antioxidant activity and IC50 value of the ethanol extract of ganitri (Elaeocarpus ganitrus Roxbs.) leaves. Ganitri leaf ethanol extract activity test was carried out using DPPH method with vitamin C as a standard. Antioxidant activity was determined as a decreas in the absorbance of DPPH at 517 nm wavelength after an addition of the extract with the concentrations of 20, 40, 80, and 100 ppm. The antioxidant acitivity measurement of the ganitri leaf extract showed that the linier regression equation obtained was y = 0.3669x + 29.546, r = 0.4573 while the IC50 value was 54,12 ppm. Based on the result, it is concluded that the ethanol extract of ganitri (Elaeocarpus ganitrus Roxb.) leaf showed was categorized as strong antioxidant.

scholarly journals A Review: Testing Antioxidant Activity on Kawista Plants (Limonia acidissima L.) in Indonesia

2021 ◽  
Vol 9 (F) ◽  
pp. 281-287
Author(s):  
Syamsuri Syakri ◽  
Nur Azizah Syahrana ◽  
Asrul Ismail ◽  
Karlina Amir Tahir ◽  
Anshari Masri
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Oxidation Process ◽  
The Body ◽  
Test Method ◽  
Exclusion Criteria ◽  
Average Value ◽  
Literature Searches ◽  
Ic50 Value ◽  
Limonia Acidissima

BACKGROUND: Antioxidants are natural ingredients that can block the oxidation process of free radicals that enter the body so that damage to body cells can be prevented. Kawista plants are one of the plants that have antioxidant activity. AIM: This article review aims to determine the compounds that have antioxidant activity found in Kawista plants, the method used in testing antioxidants on Kawista plants, and the IC50 value found in antioxidant testing on Kawista plants. METHODS: The method used in this study is the Population, Intervention, Control, and Outcome method through inclusion and exclusion criteria using the keywords “Antioxidant activity of Limonia acidissima,” “Antioxidant activity of Feronia limonia,” “Antioxidant activity of Kawista,” “Testing Antioxidants in L. acidissima,” and “L. acidissima” with databases used for literature searches. RESULTS: This review shows that Kawista plants have antioxidant activity of various compounds based on the test method used. CONCLUSION: Compounds in Kawista plants that have antioxidant activity are phenols, especially phenolic acids, flavonoids, flavonols, triterpenoids, saponins, tannins, terpenes, steroids, alkaloids, and glycosides. Kawista plant activity tested had IC50 with an average value of very strong (16.45 g/mL), strong (77.85 g/mL), moderate (135.02 g/mL), weak (196.67 g/mL), and very weak (751.89 g/mL).

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