scholarly journals Maternal and neonatal outcome of vertical transmission in pregnant women during COVID-19 pandemic at Quetta, Balochistan; developing a regional consensus on existing evidence.

2021 ◽  
Vol 28 (05) ◽  
pp. 742-748
Author(s):  
Nadeem Ahmed Sheikh ◽  
Faiza Khanum ◽  
Erum Pervaiz ◽  
Kanwal Nadeem
Keyword(s):  
Polymerase Chain Reaction ◽  
Pregnant Women ◽  
Real Time ◽  
Gestational Hypertension ◽  
Adverse Pregnancy Outcome ◽  
Well Being ◽  
Military Hospital ◽  
Nasopharyngeal Swab ◽  
Chain Reaction ◽  
Polymerase Chain

Objectives: To evaluate deleterious impact of novel coronavirus infection 2019 (COVID-19), on both maternal and fetal well being during pregnancy. Study Design: Hospital-Based Cross-sectional Survey. Setting: Department of Gynecology & Obstetrics, Combined Military Hospital, Chiltan Road, Quetta (Balochistan); Department of Gynecology & Obstetrics, Frontier Corps Hospital, Quetta Cantonment (Balochistan), and the Department of Otorhinolaryngology (ENT), Combined Military Hospital, Chiltan Road, Quetta (Balochistan). Period: March 2020 till July 2020. Material & Methods: Careful history, clinical and obstetrical examination radiology and real time polymerase chain reaction of nasopharyngeal swab were carried out in pregnant patients presenting for childbirth. Deliveries were conducted through spontaneous vaginal birth and caesarean section as per indication. Neonatal evaluation and nasopharyngeal swab for COVID-19 real time polymerase chain reaction in all delivered fetuses were performed. Results: 516 pregnant women underwent spontaneous vaginal delivery and caesarean sections at these hospitals during the study duration. 4.06% (n=21) were confirmed as COVID-19 infected. All of the fetuses born to these infected mothers were delivered healthy and COVID-19 negative, except for one preterm fetus born at 22nd week of gestation on account of maternal gestational hypertension. Conclusion: Trans-placental spread of COVID-19 infection to the fetus is unlikely. However, the infection places a pregnant mother at much higher risk to develop complications which may occasionally lead to adverse pregnancy outcome.

The results of the examination of cervical-vaginal microbiota in pregnant women with threatened preterm birth using a real-time polymerase chain reaction

2018 ◽  
Vol 11_2018 ◽  
pp. 50-59 ◽  
Author(s):  
Dobrokhotova Yu.E. Dobrokhotova ◽  
Bondarenko K.R. Bondarenko ◽  
Gushchin A.E. Gushchin ◽  
Rumyantseva T.A. Rumyantseva ◽  
Dolgova T.V. Dolgova ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Preterm Birth ◽  
Pregnant Women ◽  
Real Time ◽  
Vaginal Microbiota ◽  
Chain Reaction ◽  
Polymerase Chain

Accuracy of a rapid real-time polymerase chain reaction assay for diagnosis of group B Streptococcus colonization in a cohort of HIV-infected pregnant women

2016 ◽  
Vol 30 (9) ◽  
pp. 1096-1101 ◽  
Author(s):  
Maria Isabel S. Gouvea ◽  
Esau C. Joao ◽  
Maria de Lourdes B. Teixeira ◽  
Jennifer S. Read ◽  
Sergio E. L. Fracalanzza ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pregnant Women ◽  
Real Time ◽  
Polymerase Chain Reaction Assay ◽  
Group B Streptococcus ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Group B

scholarly journals COMPARISON OF MOLECULAR AND SEROLOGICAL TESTS FOR SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS 2 (SARS-COV-2) IN POST-EXPOSURE EMPLOYEES OF THE NEPHROLOGY DEPARTMENT

2020 ◽  
Vol 73 (12) ◽  
pp. 2572-2575
Author(s):  
Marlena Kwiatkowska ◽  
Inga Chomicka ◽  
Jolanta Malyszko
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Serological Test ◽  
Qualitative Assessment ◽  
Nasopharyngeal Swab ◽  
Molecular Testing ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Post Exposure ◽  
Polymerase Chain

Introduction: A novel coronavirus SARS-CoV-2 RNA, detected by reverse-transcription polymerase chain reaction (RT-PCR) was identified as the cause of a cluster of pneumonia cases in Wuhan, China. It rapidly spread, at first in China, then resulting in an epidemic in other countries throughout the world. One of such controversial topics is the issue of diagnostics and interpretation of test for COVID-19. According to Polish and global guidelines, the basis for diagnosis is molecular testing – real-time reverse transcriptasepolymerase chain reaction (RT-PCR). Taking all these data into consideration, the aim of the study was to compare RT-PCR with serological test in our employees post-exposure. According to Polish and global guidelines, the basis for diagnosis is molecular testing, real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The aim: To compare RT-PCR with serological test in our employees post-exposure. Material and methods: 79 employees of the Clinic, 19 men and 60 women in the age range 27-69 years were evaluated. Tests were begun four days after information about the positive test in our „Employee 0” and lasted for 7 days. At first, we made RT-PCR tests on the specimen from nasopharyngeal swab. Then, we accomplished rapid antibodies tests. This test is based on the qualitative assessment of the presence of IgM and IgG antibodies by immunochromatography using a sample of capillary blood from the fingertip. Results: All the tests were negative. No employee developed symptoms during the 7-day follow-up after the end of the tests. Conclusions: As routine tests for patients have been implemented widely, but similar solutions for employees have not gained popularity. Use of personal protective equipment (PPE) e.g. facemask and shields, transparent screens, disposable medical uniforms, minimalization the contact time, increasing distance from both colleagues and patients (if possible), and strictly follow sanitary procedures largely contributed to the absence of illness in the surveyed group of employees.

Clinical Case: SARS-CoV-2 Reactivation After a Heavy Workout

2020 ◽  
Vol 47 (3) ◽  
pp. 38-40
Author(s):  
S. Marchev ◽  
N. Hadzhimineva ◽  
A. Simidchiev
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Clinical Case ◽  
Clinical Symptoms ◽  
Strenuous Exercise ◽  
Nasopharyngeal Swab ◽  
Shortness Of Breath ◽  
Swab Sample ◽  
Chain Reaction ◽  
Polymerase Chain

AbstractWe present a clinical case of a patient with reactivation of SARS-CoV-2 infection after strenuous exercise in the gym. Reactivation of the virus was confirmed after detecting SARS-CoV-2 in a nasopharyngeal swab sample using real-time polymerase chain reaction (PCR). The clinical symptoms of the patient – fever and shortness of breath, as well as the presence of IgM antibodies further confirmed the diagnosis. The subjective complaints of persistent shortness of breath for more than a few weeks were noted.

Non-invasive fetal RHD genotyping by real-time polymerase chain reaction using plasma from D-negative Brazilian pregnant women

2007 ◽  
Vol 75 (1) ◽  
pp. A8-A9 ◽  
Author(s):  
P.A. Chinen ◽  
L.M.M. Nardozza ◽  
L. Camano ◽  
A.F. Moron ◽  
D.B.S. Pares ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pregnant Women ◽  
Real Time ◽  
Chain Reaction ◽  
Non Invasive ◽  
Polymerase Chain
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