scholarly journals ETIOLOGICAL AND PATHOLOGICAL STUDY OF EPIDEDE/lY'I‘IS IN GOATS IN BAGHDAD CITY

2022 ◽  
Vol 21 (1) ◽  
pp. 44-60
Author(s):  
Amir S.R. Al-Obaidil ◽  
Mohammed J. Alwan ◽  
Bushrah ‘ I. Al-Khaisi

Testes from 700 male goats aged l - 3 years , slaughtered at Baghdad abatteir, were examined during 6 months period. Histological and bacteriological examinations were done when gross lesions were observed. The results revealed that epidedimytis was observed in 21 (3%) of the examined animals ; 16 (2.3%) cases were unilaterally affected and S (0.7%) cases were bilaterally affected. Bacteriological isolations from infected organs included : Corynebacterium ovis (6 isolates) and Actinomyces pyogenes (3 isolates) both constituted the majonty of isolates ; as well as E. coli (4 iso-lates), and Pseudomonas aeruginosa , Staphylococcus epidermidis, Cory. bovis., Staph. aureus, Cory , ulcerams &. Yersinia pseuclotuberculosis ( 2 isolates of each ) and Campylobacter fetus (1 isolate). Histopathological examination showed 6 pathological patterns of epididmytis including acute epididymitis (14.2% of infected cases ) , subacute nomsuppurative epididymilis (9.5% od cases ) , chronic suppurative epididymitisd (28.5% of cases ), chronic non—suppurative epididymitis (19% of cases) and spermatic granuloma (14.2% of cases ).

2017 ◽  
Vol 1 (2) ◽  
pp. 48-60
Author(s):  
A.G. Salmanov ◽  
A.V. Rudenko

Мета роботи — вивчити резистентність до антибіотиків бактеріальних збудників інфекцій сечових шляхів (ІСШ), виділених у пацієнтів урологічного стаціонару в м. Києві. Матеріали і методи. Досліджено 1612 штамів бактерій, виділених із сечі хворих з ІСШ (цистит, уретрит, пієлонефрит), госпіталізованих в урологічне відділення ДУ «Інститут урології НАМН України» у м. Києві протягом 2016 р. Серед пацієнтів переважали жінки — 1201 (74,5 %). Вік хворих становив від 17 до 74 років. Для збору даних використано медичну документацію лікарні. Мікробіологічні дослідження виконано у лабораторії мікробіології ДУ «Інститут урології НАМН України». Аналізували результати культурального дослідження зразків сечі, зібраних за наявності клінічних ознак ІСШ. Дослідження клінічного матеріалу та інтерпретацію отриманих результатів проводили загальноприйнятими методами. Вивчено чутливість уропатогенів до 31 антибіотика дискодифузійним методом відповідно до рекомендацій Інституту клінічних та лабораторних стандартів США (Clinical and Laboratory Standards Institute (CLSI)). Результати та обговорення. Аналіз мікробного спектра сечі виявив домінування серед уропатогенів штамів Escherichia coli (32,0 %), Enterococcus faecalis (19,5 %), Klebsiella pneumoniae (10,9 %), Staphylococcus epidermidis (8,9 %), S. haemolyticus (6,5 %) та Pseudomonas aeruginosa (6,4 %). Частка Enterococcus faecium, Enterobacter aerogenes і Streptococcus viridans становила відповідно 2,5, 2,2 і 1,6 %, Enterobacter cloacae, Klebsiella oxytoca, Acinetobacter baumannii, Proteus vulgaris та Providencia rettgeri — менше 1,0 %. У більшості випадків (69,7 %) мікроорганізми виділено у монокультурі, у решті випадків — у мікробних асоціа- ціях. Високу резистентність до тестованих антибіотиків виявили штами E. aerogenes (45,1 %), E. cloacae (45,7 %), E. faecium (40,9 %), E. faecalis (40,7 %), E. coli (39,9 %), P. aeruginosa (34,0 %), K. pneumoniae (28,6 %). Найбільш активними до уропатогенів були іміпенем (E. coli — 87,6 %, P. aeruginosa — 75,7 %, E. cloacae — 67,3 %, E. aerogenes — 72,6 %, K. pneumoniae — 93,2 %), меропенем (E. coli — 89,1 %, P. aeruginosa — 76,7 %, K. pneumoniae — 82,6 %), лефлоцин (E. coli — 74,5 %, ентерококи — 78,7 %, P. aeruginosa — 76,7 %, E. cloacae — 73,9 %, E. aerogenes — 80,4 %, K. pneumoniae — 83,5 %), амоксицилін/клавуланат (ентерококи — 84,6 %), фурагін (ентерококи — 82,6 %), цефоперазон (K. pneumoniae — 89,2 %, P. aeruginosa — 73,8 %), цефтріаксон (K. pneumoniae — 80,1 %). Висновки. Антибіотикорезистентність збудників ІСШ — важлива терапевтична проблема. Найбільшою активністю до уропатогенів характеризуються іміпенем, меропенем, лефлоцин, амоксицилін/ клавуланат, фурагін, цефоперазон, цефтріаксон, які можна розглядати як препарат вибору для призначення стартової терапії ІСШ. Необхідно здійснювати постійний моніторинг за резистентністю до дії антибіотиків. Політику використання антибіотиків у кожному стаціонарі слід визначати залежно від локальних даних щодо резистентності до протимікробних препаратів.


1967 ◽  
Vol 5 (10) ◽  
pp. 39-40

Gentamicin sulphate (Cidomycin - Roussel; Genticin - British Schering) is an antibiotic related to the kanamycin group. It is produced by fermentation from an actinomycete, Micromonospora purpurea, and has a wide and unusual range of antibacterial activity.1 2 Most strains of E. coli, the Klebsiella-Aerobacter group, and all species of Proteus are highly or moderately susceptible to the drug, as are the smaller number of Shigella and Salmonella species so far tested. In this respect its range resembles that of kanamycin but, in addition, gentamicin is active against most strains of Pseudomonas aeruginosa (pyocyanea), thus combining the antibacterial activity of kanamycin with that of the polymyxins (to which Proteus is resistant). Gentamicin is also active against Staph. aureus, but streptococci are only moderately sensitive, and enterococci are often resistant.


Author(s):  
Miladys Esther Torrenegra Alarcón ◽  
Nerlis Paola Pájaro ◽  
Glicerio León Méndez

Se evaluó la actividad antibacteriana in vitro de aceites esenciales de diferentes especiesdel género Citrus frente a cepas ATCC de Staphylococcus aureus, Staphylococcus epidermidis,Klebsiella pneumoniae, Pseudomonas aeruginosa y Escherichia coli, determinandola concentración mínima inhibitoria (CMI) y la concentración mínima bactericida(CMB). Las bacterias se replicaron en medios de agar y caldos específicos. Se determinóel momento de máxima densidad óptica (DO620) para emplearlo como tiempode incubación; luego se hicieron pruebas de evaluación de sensibilidad con la exposiciónde las cepas a concentraciones a 1000 g/mL del extracto en caldo. Para solubilizarse empleó dimetilsulfóxido (DMSO) al 1%. Posteriormente, se le determinó laconcentración mínima inhibitoria mediante metodologías de microdilución en caldoy la concentración mínima bactericida. Encontrándose una actividad de los aceitesesenciales del género Citrus, con valores de CMI ≥ 600 mg/mL frente a S. aureus,S. epidermidis, K. pneumoniae, P. aeruginosa y E. coli. En función a los resultados obtenidos,se concluye que las diferentes especies del género Citrus son consideradas comopromisorias para el control del componente bacteriano.


2012 ◽  
Vol 36 (0A) ◽  
pp. 13-19
Author(s):  
احمد محمد تركي

The present study is conducted to in restigate the bacteria Pseudomonas aeruginosa, the impact of ultraviolet on the bacterial isolates under study and the resistance of these isolates to ultraviolet are studied in comparison to two standard isolates ( E . coli and Staphylococcus aureus ) which are considered sensitive to ultraviolet . The natures of the resistance of the isolates, under study, are also being investigated against the different antibiotics. The isolates are subjected to a test to examine their sensitivity to (12) types of antibiotics used routinely in the treatment of various infection of these bacteria. They are (streptomycin , cephalothin ,Gentamycin , cefotaxime ,nitrofurantion ,ampicillin, amoxicillin, rifampin, lincomycin, tetracycline, erythromycin and ciprofloxacin ).The lowest concentration installer ( MIC ) is also testified in accordance with six types of antibiotics (streptomycin, cefotaxime , rifampin , nitrofurantion , Gentamycin , amoxicillin ).The biologic effectiveness of the overlap between the bacterial isolates , under study, is examined against four bacteria (klebseilla pneumonia , Staphylococcus aureus , Enterobacter , Proteus ) The result of using the ultraviolet with different wavelength show the ability of the five local isolates used to resistance of ultraviolet reaching (180 s.) in comparison to the isolates E.coli and staph. aureus in which the ratio of killing is %100 at a time of exposing 40 , 60 sec. respectively. The results indicated that the five local bacterial isolates have high resistance to the most tested antibiotics, It is shouted that all of them have resistance to (erythromycin , tetracycline , lincomycin , Gentamycin ) but they are sensitive towards antibiotic streptomycin . as for the other antibiotics , over can find that the isolates are varied of them for being resisting or sensitive towards them .The results of testing the inhabited effectiveness of the five bacterial isolates towards some other bacterial isolates show the efficiency of the five local isolates in the inhabitation of growth of the five studied bacterial isolates.


1970 ◽  
Vol 25 (1) ◽  
pp. 9-16 ◽  
Author(s):  
T Ferdausi ◽  
MG Haider ◽  
KJ Alam ◽  
MA Baki ◽  
MM Hossain

Pathological conditions in lungs of slaughtered goats were studied. Sixty lungs were examined and tissue samples and swabs obtained for histopathology and bacterial isolation, respectively. The prevalence of lung diseases was 58.3% (n=35). Gross lesions were categorized into: (a) haemorrhage and congestion 25% (b) emphysema 21.7% (c) hepatization 3.3% and (d) granulomatous nodules about 1 mm diameter 8.3%. On histopathological examination, 10 types of lesions were found: (a) bronchitis 6.7%, (b) small cell anaplastic carcinoma 3.3%, (c) pneumonia 6.7%, (d) interstitial pneumonia 15%, (e) emphysema 6.7%, (f) bronchopneumonia 3.3%, (g) purulent pneumonia 5%, (h) haemorrhagic pneumonia 3.3%, (i) pulmonary adenomatosis 1.7% and (j) no lesions 6.7%. Pasteurella sp. (11.7%), Escherichia coli (E. coli; 6.7%), Staphylococcus sp. (36.7%) and Bacillus sp. (3.3%) were isolated from the lungs. Pasteurella sp. was found in haemorrhagic pneumonia, interstitial pneumonia, small cell anaplastic carcinoma and bronchitis, followed by Bacillus sp. in haemorrhagic pneumonia, E. coli in interstitial pneumonia and pulmonary adenomatosis and Staphylococcus sp. from emphysema, bronchopneumonia, pneumonia, bronchitis and purulent pneumonia. DOI: 10.3329/bvet.v25i1.4613 Bangl. vet. 2008. Vol. 25, No. 1, 9-16


1989 ◽  
Vol 102 (3) ◽  
pp. 447-458 ◽  
Author(s):  
G. R. Smith ◽  
D. Till ◽  
L. M. Wallace ◽  
D. E. Noakes

SUMMARYNecrobacillosis is caused byFusobacterium necrophorum(FN), but other organisms are often present in the lesions. Their possible role was studied in experiments made with a virulent FN strain which, by itself, produced fatal necrobacillosis in mice provided that large doses ( > 106organisms, subcutaneously) were given. Mice were inoculated subcutaneously with FN suspended in sub-lethal doses (0·1 ml) of undiluted or diluted broth cultures of other bacteria. Undiluted culture of a strain ofEscherichia colireduced the infective dose of FX to < 10 organisms: in the necrobacillosis lesions that developed, fusobacteria greatly outnumberedE. coli. A heat-killed preparation or sterile filtrate ofE. coliculture had little if any effect on FN.Citrobacter freundiiand comparativelv small numbers ofCorynebacterium (Actinomyces) pyogenesproduced effects similar to that ofE. coli. An α-haemolvtic streptococcus.Pseudomonas aeruginosa.Bacteroides fragilisandFusobacterium nudeatumalso enhanced the infectivity of FN. though less strikingly thanE. coli. FN increased the persistence invivoof the α-haemolytic streptococcus andB. fragilis, and enabled the latter to multiply profusely.


2013 ◽  
Vol 12 (2) ◽  
pp. 119
Author(s):  
S. O. Youkhana

A pathological study of bracken fern ( Pteridium aquilinium ) toxicity was conducted in rats. Twenty albino rats were randomly divided in to 4 groups. The first three groups were received diet containing 10 %, 20 % and 30 % bracken fern powder respectively. The fourth group was served as control. The experimental rats were sacrificed after one month. Gross and histopathological examination reveal lesions in many organs . Gross lesions revealed congestion of all internal organs, while the histopathological lesions were characterized by spongiosis, perivascular cuffing and astrocytosis in the brain. Swollen hepatocytes accompanied by vacuolation and fatty degeneration in the liver. Suppurative pneumonia with infiltration of inflammatory cells in the lungs. In the intestine, the villi were fused with a proliferation of cryptal enterocytes. Interstitial nephritis associated by degenerative changes in the renal tubular epithelial cells. Acute cystitis with edematous lamina propria and neutrophilic infiltration were seen in the urinary bladder. These lesions were more sever in the group 3 incomparism with group 1 and 2 . No lesions were observed in the control group


Author(s):  
Y. H. Al Tarazi

Les publications concernant la pneumonie chez le dromadaire sont en général rares. Dans cette étude, 284 poumons de dromadaires, abattus entre 6 mois et 10 ans d’âge et provenant du nord de la Jordanie, ont été examinés. La prévalence de la pneumonie a été de 10,2 p. 100. Les lésions pathologiques des poumons atteints de pneumonie ont été classées selon qu’elles indiquaient la présence de la bronchopneumonie proliférative chronique, de la pleuropneumonie chronique ou de la pneumonie interstitielle. Les abcès du poumon ont aussi été enregistrés. La broncho-pneumonie proliférative chronique (20,69 p. 100) et la pleuropneumonie chronique (6,9 p. 100) ont été plus fréquentes chez les dromadaires âgés (10 ans environ), alors que la pneumonie interstitielle (58,6 p. 100) et les abcès du poumon (10,34 p. 100) ont été plus fréquents chez les jeunes dromadaires (âgés de 6 mois à 4 ans). Mannheimia haemolytica et Pseudomonas aeruginosa ont été les espèces les plus fréquemment isolées dans le cas de broncho-pneumonie proliférative chronique et de pleuropneumonie chronique, alors que, dans les cas de pneumonie interstitielle, c’étaient Escherichia coli et Klebsiella spp. qui l’ont été le plus. Dans les cas d’abcès du poumon c’étaient Staphylococcus aureus, Actinomyces pyogenes et des streptocoques hémolytiques qui ont été le plus fréquemment observés. Au total, 75 espèces bactériennes ont été isolées de 29 poumons. Les plus fréquentes ont été E. coli (26,66 p. 100), Klebsiella spp. (14,66 p. 100), Pseudomonas aeruginosa (12 p. 100), Staphylococcus aureus (10,66 p. 100), Mannheimia haemolytica (6,66 p. 100) et Actinomyces pyogenes (6,66 p. 100). L’espèce la plus souvent observée parmi les Klebsiella identifiées a été Klebsiella ozaenae.


2017 ◽  
Vol 45 ◽  
pp. 4
Author(s):  
Ruben Horn Vasconcelos ◽  
Windleyanne Gonçalves Amorim Bezerra ◽  
Raul Antunes Silva Siqueira ◽  
Pedro Henrique Quintela Soares De Medeiros ◽  
Ricardo Barbosa Lucena ◽  
...  

Background: Rock pigeon (Columba livia) is an exotic and invasive species of the Columbidae family found in several cities around the world, including Brazilian cities, and close to humans. This species is found in large populations often in public locations, where people may have contact. Several studies have demonstrated the isolation of human pathogens from these birds. However, there are scarce studies describing infections by Gram-negative bacteria to which pigeons are susceptible. Therefore, this report aimed to describe a case of natural coinfection of Pseudomonas aeruginosa and Enteroaggregative Escherichia coli in a feral pigeon.Case: A sick feral pigeon was delivered at the Laboratory of Ornithological Studies, State University of Ceará, Fortaleza, Brazil. Due to poor prognosis, the individual was euthanized with ketamine via intravenous injection and submitted to necropsy, in which samples were collected for microbiological and histopathological procedure. The procedure was performed aseptically and samples were collected from intestine, liver, spleen, heart and lung. Bacterial isolation was performed with culture media selective for Gram negative bacteria and strains were identified biochemically. Histopathological examination was performed with conventional method and slides were stained with hematoxylin and eosin. DNA from E. coli isolates was extracted with simples boiling method and submitted to uniplex conventional polymerase chain reaction (PCR) to diagnose diarrheagenic pathotypes with specific primers for the following genes: aaiC, aatA, eaeA, stx1, stx2, eltB, estA and ipaH. Escherichia coli was isolated from the intestine, liver, spleen and lung, while Pseudomonas aeruginosa was present in liver, spleen and lung. E. coli strains from liver and spleen were positive for the aaiC gene, which is a diagnostic gene for Enteroaggregative Escherichia coli (EAEC) pathotype. Necropsy revealed the presence of several caseous lesions around the head of the bird, cellulitis in the abdominal region, hepatomegaly, splenomegaly, hemorrhagic intestine and pericarditis. Microscopical findings were most intense in liver and lung samples, presenting vascular and inflammatory alterations. Histopathological alterations indicated an acute presentation of the infections and several lesions in different organs, demonstrating the septicemic characteristic of the microorganisms involved in this case.Discussion: Both pathogens in this report were identified mostly in the same organs, which may indicate that there was an association in the pathogenesis. However, the entry routes of infection in this case may have been different, considering that Pseudomonas aeruginosa was not isolated from the intestine. The histopathological findings were not pathognomonic for either pathogen. However, some characteristic lesions were observed, such as cellulitis, which is commonly attributed to Escherichia coli. EAEC strains are human pathogens that cause acute and persistent diarrhea around the world. These microorganisms could be potentially transmitted to humans, as suggested by other studies with feral pigeons and enteric pathogens. However, the absence of reports of pigeon-transmitted diarrheagenic infections in humans may suggest that pigeons are only reservoirs of these pathogens. In addition, as this report demonstrates, these birds may also suffer from these infections. In conclusion, the free-living pigeon presented a natural occurring case of coinfection by Pseudomonas aeruginosa and Enteroaggregative Escherichia coli. Both are human opportunistic pathogens, and may have public health implications


2021 ◽  
Vol 2 (2) ◽  
pp. 3299-3310
Author(s):  
Galicia Grande Alejandro ◽  
Villagrán Padilla Claudy ◽  
Suárez Albores Patricia ◽  
Ruiz Tagle Alejandro ◽  
Nava Rodriguez I.

Las infecciones intrahospitalarias (IHH) se consideran de gran importancia en pacientes internados en el hospital de traumatología y ortopedia IMSS debido a que es uno de los grandes problemas que se presenta en los pacientes y experimenta en algún momento durante su estancia. El cultivo bacteriológico es un método específico y confiable para su diagnóstico. Como propósito de este estudio se tiene el determinar la frecuencia de microorganismos patógenos aislados en cultivos de infecciones a pacientes internados en el hospital de traumatología y ortopedia IMSS de la ciudad de Puebla. Se realizó un estudio de tipo transversal, prospectivo, y observacional para 844 muestras de infecciones provenientes de diversas áreas anatómicas provenientes de pacientes que se encuentran internados en el hospital de traumatología y ortopedia IMSS de la Ciudad de Puebla, en el periodo enero 2015 a diciembre 2015. A cada muestra se le realizó un cultivo bacteriológico, así mismo se procedió a la identificación del agente microbiano, y realización de antibiograma. Respecto al cultivo bacteriológico, el microorganismo más aislado fue E. coli con un 23%, seguido de Staphylococcus aureus 17%, Staphylococcus epidermidis 12%, Staphylococcus haemolyticus 9%, Pseudomonas aeruginosa 5%, Klebsiella pneumoniae 5%, Acinetobacter baumanii 5%, Enterobacter cloacae 5%, Enterobacter faecalis 4%, Staphylococcus coagulasa neg. 2%, Streptococcus pyogenes 19%, Morganella morgani 2%, Proteus mirabilis 1%, Pseudomonas sp. y Candida albicans ambas con 0.9%, Serratia marcescens 0.5%, Citrobacter freundii 0.4%. Bacterias de interés clínico ya que estas son causantes de infecciones que complican la salud de los pacientes. El antibiograma fue realizado a los cultivos positivos conforme a las solicitud requisitada, dio como resultado una baja sensibilidad en bacterias tanto grampositivas y gramnegativas, esto demuestra que las bacterias están mostrando resistencia a los antibióticos, debido a la administración y mal manejo de antibióticos de amplio espectro en los pacientes ya que en la mayoría de los casos el médico inicia el tratamiento sin tener un cultivo y antibiograma previo para tener un control de los medicamentos administrados. De acuerdo con los resultados obtenidos, se concluye que los cultivos bacteriológicos nos dieron información importante con respecto a este estudio en cuanto a que la presencia de E. coli nos da a entender que hay malas prácticas de higiene en cuanto a procedimientos médicos y manejo de los pacientes, pero también nos muestra un problema actual el cual es la resistencia de las bacterias a los antibióticos, siendo este un problema urgente que se debe de erradicar con información y buenas prácticas higiénicas.


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