scholarly journals Comparative Study of Laser- attenuated and Gamma attenuated irradiated larval Vaccines of Dictyocaulus filaria in goats.

2021 ◽  
Vol 25 (1) ◽  
pp. 91-104
Author(s):  
AL- Mashhadani, F.J. K. ◽  
AL-Samarrae, S.A. G

A comparative trial between the effect of 3rd stage Dictyocaulus filaria larval vaccine attenuated by continuous emission of visible Helium – Neon Laser of ImW and wave length of ( 632.8 ) nm of (5) minutes exposure that of gamma attenuated larvae at 0.5 k. gray ( Co as radiation Source ) was designed. Each of the two attenuated larval vaccines was given to a group of ( 5 ) kids each . Double immunization doses at ( 35 ) days interval from each vaccine were given orally. The first dose contained ( 1000 ) larvae and the second one ( 2000 ) for each . A challenge dose of ( 100 ) non- irradiated larvae /kg body weight was given after ( 5 ) week to the vaccinated groups and a control non- vaccinated (5 kids ) 3rd group. All animals were slaughtered after 6 weeks post challenge.  Results showed that both vaccines revealed 80.7% and 78.1% protection respectively for worm burden as compared to control. Laser attenuated vaccine exhibited a statistically significant inhibition in the fecundity of female worms and larvae secretion in faeces in comparison with the gamma irradiated vaccine. 

2001 ◽  
Vol 25 (1) ◽  
pp. 63-76
Author(s):  
AL- Samarrae , S.A. G ◽  
AL- Mashhadani, F. J. K.

Irradiated D. filaria 3rd stage larvae with cantinuous emission of visible Helium- Neon Laser of ImW and a wave length of ( 632.8 ) nm. for three different exposure times of 5 , 10 and 15 minutes were used to vaccinate three groups of kids of five each at double immunization doses orally. The first dose contained ( 1000 ) larvae and the second was ( 2000 ) larvae at an interval of ( 4 ) weeks between then . After ( 5 ) weeks a challenge dose of ( 100 ) larvae per kg. Body weight ( non irradiated ) was given to all the groups in addition to a control group ( 4th ) of ( 5 ) kids . All animals were slaughtered after ( 6 ) weeks post-challenge. Results showed that the attenuated larvae at ( 5 ) minutes exposure to Laser irradiation was the most appropriate one and can be used as an attenuated irradiated larval vaccine effectively and safely. The assessment was based on clinical signs, body weight gains ,eosinophil counts of blood, fecal larval counts , mean worm burden and female sexual maturity, and gross pathological changes including lung lesion scores.


2000 ◽  
Vol 24 (2) ◽  
pp. 24-34
Author(s):  
S. A. G AL-S amarrae ◽  
F. J. K. AL- Mishhadani

Attenuated 3rd stage larvae of Dictyocaulus filaria, by continuos emission of visible Helium- Neon laser of 1mW and a wave length of 632.8 nm for 2.5,5,10 and 15 minutes, were dosed twice orally to four groups of five kids each. The first dose contained 1000 larvae and after five weeks a second dose of 2000 larvae was given . A 5 th group of five kids was left as control.  Results showed that attenuation at 2.5 min. exposure was insufficient as indicated by the increase in respiratory rate, eosinophilia and shading of larvae in the feaces. The results indicated that attenuation of Dictyocaulus filaria larvae at (5, 10 , and 15) min. exposure to laser radiation were efficient and safe to be used as attenuated vaccine .


1966 ◽  
Vol 54 (10) ◽  
pp. 1290-1294 ◽  
Author(s):  
A.L. Bloom ◽  
D.L. Wright

Author(s):  
M.V. Chirkin ◽  
◽  
S.V. Ustinov ◽  
V.Yu. Mishin ◽  
◽  
...  

1990 ◽  
Vol 94 (6) ◽  
pp. 822-826 ◽  
Author(s):  
Ann F. Haas ◽  
R. Rivkah Isseroff ◽  
Ronald G. Wheeland ◽  
Pamela A. Rood ◽  
Phillip J. Graves

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