scholarly journals Bacteriological and heavy metal assessment of a tropical crude oil- polluted soil, water and sediment

2021 ◽  
Vol 16 (2) ◽  
pp. 159-165
Author(s):  
Maryjoy Chidinma Maduwuba ◽  
Gideon Chijioke Okpokwasili ◽  
Abiye Anthony Ibiene

Crude oil-polluted environment serves as a reservoir of microbial species, especially bacteria. Determining these bacterial genera is of great benefit to environmental assessment and recovery. This study evaluated the bacteria and selected heavy metals present in crude oil-polluted sites in K-dere community, Ogoniland. The study was carried out at the Department of Microbiology, University of Port Harcourt, Rivers State, Nigeria. Crude oil-polluted soil, water, and sediment samples were collected from K-dere community, Ogoniland. The pH and heavy metals composition of each sample was determined using pH meter and spectrophotometer, respectively, while isolation and biochemical characterization were done to evaluate the heterotrophic and hydrocarbon utilizing bacteria present in each sample. Results revealed that iron (Fe) had the highest concentration in the soil (898.77±0.022 mg/kg) and sediment (1556.7±0.163 mg/kg) samples, while lead (Pb) had the lowest overall concentration. The concentration of total petroleum hydrocarbon (TPH) (10410.5 mg/kg) in soil sample exceeded the DPR standard limit of 5000 mg/kg for soil. The results of total culturable heterotrophic bacterial counts (TCHBC) and total culturable hydrocarbon utilizing bacterial counts (TCHUBC) showed that soil and sediment samples had the highest TCHBC and TCHUBC values of 2.66 ± 0.03 x 108 CFU/mL and 4.7 ± 0.14 x 107 CFU/mL respectively. Morphological and biochemical characterization of the isolates revealed the presence of Pseudomonas spp, Bacillus spp, Acidiphilium spp, Mycobacterium spp and Leptospirillum spp in the samples with Pseudomonas spp having the highest percentage occurrence. This study has revealed the presence of useful bacterial species in the sampled sites which can be harnessed for an in situ cleaning of crude oil- contaminated site, especially in the tropical region.

2020 ◽  
Vol 65 (2) ◽  
pp. 17-37
Author(s):  
Georgiana Grosu ◽  
◽  
Carmen Andreea Roba ◽  
Ramona Bălc ◽  
Maria Lucia Bizău-Cârstea ◽  
...  

The present study was conducted in the proximity of a contaminated site from Cluj-Napoca city (Cluj County, Romania), where metal processing activities have been carried out for decades. Metal content and physico-chemical parameters were analyzed in soil, water and sediment samples, while organic matter (OM) and total organic carbon (TOC) was additionally analyzed for the soil samples. The sources of heavy metals were evaluated based on multivariate statistical analysis, while the soil and sediment contamination degree was assessed based on specific pollution indices. The calculated indices indicated a significant pollution with Cd and Pb, which may represent a risk if the area would become a residential area. Keywords: heavy metals, contaminated site, soil pollution indices, multivariate statistical analysis, Cluj-Napoca


Author(s):  
Muibat Fashola

Introduction: Indiscriminate dumping of spent oils enriched with heavy metals has led to increase in heavy metals load in the soil. Heavy metals exert toxic effects on biodegradation of organic pollutant in cocontaminated soil and there is need to find suitable strategies for their removal. Aim: The aim of this study was to assess the heavy metals resistance capability of indigenous Bacillus species in hydrocarbon polluted soil to nickel (Ni), Cadmium (Cd), Lead (Pb) and Chromium (Cr). Materials and Methods: Heavy metal tolerant bacteria were isolated from hydrocarbon polluted soil using Luria-Berthani agar supplemented with the respective metals and spread plate techniques. The isolates were putatively identified on the basis of their colonial morphology and biochemical characteristics and their antibiotics susceptibility pattern were evaluated using disc diffusion method. Results: The maximum tolerable concentration (MTC) of the four heavy metals to the selected isolates was 2 mM. Four bacteria isolates able to withstand the MTC were putatively identified as Bacillus subtilis, Bacillus megaterium, Bacillus laterosporus and Bacillus polymyxa. Out of the four Bacillus species, only B. laterosporus did not show multiple tolerance to the tested antibiotics which show that there is correlation between heavy metal tolerance and antibiotics resistance by the isolates. Conclusion: Multiple heavy metal tolerance Bacillus spp. were isolated from crude oil polluted soil. These bacteria could be suitable agents for bioaugmentation of hydrocarbon polluted soil co-contaminated with heavy metals.


2015 ◽  
Vol 6 (2) ◽  
Author(s):  
Sophia L Sagala ◽  
Rikha Bramawanto ◽  
Anastasia R.T.D Kuswardani ◽  
Widodo S Pranowo

ABSTRACTThe study of Pb, Cd, Cu, Hg, and As and Fe in Natuna coastal waters, Riau Islands was conducted in November 2012. Water and sediment samples were collected from 35 stations by purposive sampling method and those heavy metals were analysed using Atomic Absorption Spectrophotometer, flame type (FAAS). The results showed that concentrations of total Pb, Cd, Cu, Hg, and As in surface water were below instrument detection limit (2 µg/L for Pb, Cd, and Cu; 0.02 µg/L for Hg and As). Moreover, the concentration of metal Fe in surface water was 0.021-0.054 mg/L. Concentrations of Pb and Cu in sediment were 0.05-22.67 mg/kg and 3.77-11.00 mg/kg, respectively, at which the highest concentrations were found near Binjai Estuary. Those concentrations were significantly below the standard levels set by CCME (2002) and ANZECC/ARMCANZ (2000). Concentration of Fe in sediment varied from 751.13-2309.12 mg/kg and showed similar spatial distribution to Cu-in-sediment. No standard level for Fe in waters was available. Generally, it can be concluded that Natuna coastal water was still uncontaminated.  Keywords: heavy metals, iron metal, Natuna coastal waters.


2019 ◽  
Vol 22 (2) ◽  
pp. 109
Author(s):  
Derra Alianie Tawa ◽  
Budi Afriyansyah ◽  
Muhammad Ihsan ◽  
Mohammad Agung Nugraha

Illegal tin mining or unconventional mining activities produce tailings. Tailings contain dangerous heavy metals one of which is Lead (Pb). The increasing concentration of Pb in the waters will also increase its concentration in the body of the biota, one of which is shrimp. The purpose of this study was to analyse the Pb content in gills, hepatopancreas and muscle in Penaeus merguiensis, analyse the Pb content in water and sediments, measure the ability of Penaeus merguiensis in accumulating Pb in water and sediments, and determine the safe limit for consumption. Shrimp, water and sediment samples were analysed using AAS. The results showed that the highest Pb concentration in Penaeus merguiensis was hepatopancreas> gill> meat, with an average range of 0,1897–0,4064 mg/kg, gills 0,2424-0,4770 mg/kg, and meat 0,1348-0,1636 mg/kg. The average Pb concentration in water ranged from 0,2624 to 0,5713 mg/L, while the sediment ranged from 0,2783 to 0,9760 mg/kg. The ability of Penaeus merguensis to accumulate Pb included in the low category. The value of daily intake is around 9,760-11,128 kg/week. Aktivitas penambangan timah illegal atau tambang inkonvensional menghasilkan sisa limbah buangan (Tailing). Tailing mengandung logam berat berbahaya salah satunya Timbal (Pb). Meningkatnya konsentrasi Pb di perairan akan meningkat pula konsentrasinya dalam tubuh biota, salah satunya udang. Tujuan dilakukannya penelitian ini adalah menganalisis kandungan Pb pada insang, hepatopankreas dan daging pada Penaeus merguiensis, menganalisis kandungan Pb pada air dan sedimen, mengukur kemampuan Penaeus merguiensis dalam mengakumulasikan Pb di air dan sedimen, dan menentukan nilai batas aman konsumsi. Sampel udang, air dan sedimen dianalisis menggunakan AAS. Hasil penelitian menunjukkan bahwa konsentrasi Pb tertinggi pada Penaeus merguiensis adalah hepatopankreas>insang>daging, dengan kisaran rata-rata 0,1897–0,4064 mg/kg, insang 0,2424-0,4770 mg/kg, dan daging 0,1348-0,1636 mg/kg. Konsentrasi Pb rata-rata dalam air berkisar antara 0,2624-0,5713 mg/L, sementara sedimen berkisar dari 0,2783-0,9760 mg/kg. Kemampuan Penaeus merguensis untuk mengakumulasi Pb termasuk dalam kategori rendah. Nilai asupan harian sekitar 9,760-11,128 kg/minggu.


2019 ◽  
Vol 26 (32) ◽  
pp. 33532-33540
Author(s):  
Rennan Cabral Nascimento ◽  
Yuri Jacques Agra Bezerra da Silva ◽  
Clístenes Williams Araújo do Nascimento ◽  
Ygor Jacques Agra Bezerra da Silva ◽  
Rayanna Jacques Agra Bezerra da Silva ◽  
...  

Author(s):  
Astom Mondal ◽  
Sankar Narayan Sinha

The country of India is the unique where different cultural heritage as well as festivals were found. Mainly the West Bengal state is known as various religious festivals, but Durga Puja is one of the biggest and end of this festival idol are immersed in the water bodies. Sainthia town found in the district of Birbhun, West Bengal, India which is present in the south bank of the Mayurakshi river. The idol immersion mainly effect on water as well as sediment quality of this river Mayurakshi near railway bridge at Sainthia. The study will be discussed for immersion effect of this sampling station, because in this point totally Sainthia town Durga idols were immersed. The water and sediment samples were collected at 7:30 am on 9th October 2019 during pre-immersion (before immersion) and 7:30am on 10th October 2019 during the post immersion (after immersion) in this time of periods. Mainly, the Durga idols are been made up by non-biodegrable substances such as clay, plaster of paris, cloths, paper woods, thermocols, jutes and synthetic paints etc. these are mainly toxic substances. Over all the used materials in making of idol, the thermocol is nonbiodegradable while paints contained highly toxic heavy metals like Cr, Cd and Pb. In this study has been made to analysis the physicochemical parameters like temperature, pH, TS, TDS, TSS, EC and three heavy metals like Cr, Cd and Pb on the collected water and sediment samples of the Mayurakshi river on before and after idol immersion periods of near railway bridge sampling station. The analysed results by based on various physicochemical parameters showed that the after immersion in the river Mayurakshi increases concentration of heavy metals and changes other physicochemical parameters of the both water and sediment samples. Finally concluded that, these changes of different parameters are harmful for aquatic life and human beings as well as the level of water pollution highly increases in this Mayurakshi river. So, the huge religious activities mainly causes of the water pollution where huge disturbing aquatic ecosystem.


1984 ◽  
Vol 30 (5) ◽  
pp. 565-571 ◽  
Author(s):  
P. M. Fedorak ◽  
K. M. Semple ◽  
D. W. S. Westlake

Seventy-four yeasts and 224 fungi were isolated from marine water and sediment samples taken from the Strait of Juan de Fuca and northern Puget Sound. When these isolates were grown in the presence of Prudhoe Bay crude oil, only three yeasts and 63 fungi were able to degrade some or all of the n-alkanes. None degraded the isoprenoids, pristane and phytane. Forty-seven isolates were identified as Penicillium species and of these, 39 attacked the n-alkanes in the crude oil. Twelve organisms which degrade n-alkanes were tested for their ability to mineralize [14C]naphthalene and [14C]phenanthrene which had been added to the crude oil. No 14CO2 was detected from any of the cultures containing these compounds. Capillary gas chromatographic analyses of the aromatic fractions from these 12 cultures showed no loss of hydrocarbons or sulfur hetero-cycles, indicating that they were unable to completely or partially oxidize any of the resolvable compounds in this fraction.


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