scholarly journals Changes in the compound zeta potential of erythrocyte membranes in patients with arterial hypertension and obesity

2021 ◽  
Vol 99 (5-6) ◽  
pp. 342-346
Author(s):  
V. I. Podzolkov ◽  
T. V. Koroleva ◽  
A. E. Bragina ◽  
A. I. Tarzimanova ◽  
M. G. Kudryavtseva ◽  
...  

The development of microcirculatory disorders is considered to be one of the earliest changes in the cardiovascular system with a combination of arterial hypertension (AH) and obesity. The rheological properties of blood play a significant role in the system of microcirculation. An important place in changing the rheological characteristics of blood is largely assigned to erythrocytes. Aggregation of erythrocytes is closely related to the magnitude of the surface-bound charge of their membranes or zeta potential of erythrocyte membranes. Purpose. To study the state of compound zeta potential of erythrocyte membranes in patients with hypertension and obesity. Material and methods. The study included 112 patients with AH and Index of Mass Corporal more than 30 kg/m2 (main group); the control group consisted of 25 people without AH and obesity. All patients of the main group received standard antihypertensive, lipid-lowering and hypoglycemic therapy to achieve the target levels of the indices under correction. Results. The level of compound zeta potential of erythrocyte membranes in patients with AH and obesity was significantly lower than in the control group and amounted to 1.57 ± 0.06 × 107 and 1.67 ± 0.03 × 107, respectively (p < 0.05). At the same time, in patients of the main group with an increase in AH, lower indices were noted. In patients with AH and obesity, a significant inverse correlation was found between the compound zeta potential of erythrocyte membranes and the degree of hypertension, which indicates a negative effect of hypertension on the rheological properties of blood at the microvasculature level. A correlation analysis was carried out to evaluate the association between Index of Mass Corporal and zeta potential of erythrocyte membranes in obese hypertensive patients. A negative direct correlation was revealed (r = 0.7, p < 0.05). Conclusion: a decrease in the total charge of erythrocytes can be considered as an early sign of microrheological disorders in patients with a controlled course of arterial hypertension and obesity.

2019 ◽  
Vol 15 (5) ◽  
pp. 634-640
Author(s):  
S. Yu. Nikulina ◽  
V. A. Shulman ◽  
A. A. Chernova ◽  
S. V. Prokopenko ◽  
D. A. Nikulin ◽  
...  

Aim. To study the association of single nucleotide polymorphism rs556621 (G> T) with development of stroke in patients of the East Siberian population with cardiovascular pathology and risk factors.Material and methods. The study involved 260 patients (157 men and 103 women) with stroke (mean age 57.0 [51.0-62.0]) and 272 patients (170 men and 102 women) of the control group (mean age 55.0 [51.0-62.0]). The examination of the main group included: collection of complaints, anamnesis, clinical examination, computed tomography of the brain, electrocardiography, echocardioscopy, ultrasound duplex scanning of extracranial brachiocephalic arteries, daily blood pressure and heart rate monitoring, analysis of the blood coagulation system. The patients of the main group have arterial hypertension, paroxysmal supraventricular tachycardias, dyslipidemia, atherosclerosis of the brachiocephalic arteries, disorders of the hemostatic system. The control group was studied in the framework of the HAPIEE international project. Molecular genetic research was performed by real-time polymerase chain reaction.Results. There were no statistically significant differences in the frequencies of genotypes and single nucleotide polymorphism rs556621 alleles (G>T) in the subgroup of patients with stroke and those in the control group. The frequency of the rare TT genotype among patients with stroke was 13.3%±4.16, among healthy individuals – 8.8±3.37% (p=0.1). Gender differences when comparing the frequencies of genotypes and alleles were also not detected (p>0.05). The frequencies of the TT genotype were approximately the same in the subgroup of patients with arterial hypertension (13.1%±4.22) and in the control group (7.4±5.25%; p>0.05). No significant differences were observed in the frequencies of the rare genotype of the studied polymorphism in the subgroup of patients with supraventricular tachycardias (20.0±14.37%), hypercoagulability (15.9±7.64%) and the control group (8.8±3.37%), p>0.05. A statistically significant relationship was found between the rare genotype TT of single nucleotide polymorphism rs556621 (G>T) and the development of stroke in patients with dyslipidemia and atherosclerotic lesions of the coronary arteries (p=0.041; odds ratio 1.86, 95% confidence interval 1.02-3.41).Conclusion. The genotype of TTs of single nucleotide polymorphism rs556621 (G> T) increases the risk of developing stroke in patients with dyslipidemia and atherosclerosis of the brachiocephalic arteries compared with carriers of the GG and GT genotypes. The obtained data are recommended to be considered when prescribing lipid-lowering and antithrombotic therapy. 


Author(s):  
T.I. Viun ◽  
L.M. Pasieshvili ◽  
S.V. Viun ◽  
A.S. Marchenko ◽  
O.V. Karaya

The prevalence of comorbidities has been growing for the last decades. Therefore, the detection of biomarkers for diagnostic and prognostic purposes is of great practical importance. The aim of this study was to assess the biomarkers of osteo-defficiency in the course of secondary osteoporosis in patients with comorbid chronic pancreatitis and arterial hypertension. We examined 110 patients with chronic pancreatitis: 70 of them had comorbid hypertension, and 40 patients were found as having no comorbidities. The age of patients ranged from 33.2 ± 2.1 (main group) and 32.9 ± 3.1 years (comparison group); women predominated (72.9% and 70%, respectively). The control group includes 78 healthy individuals of the same age and sex. Diagnostic investigation included studying clinical and anamnestic characteristics of patients (duration of the disease, manifestations of the course, frequency of recrudescence, fractures) and biochemical parameters of bone metabolism: osteocalcin, total bone phosphatase and tartrate-resistant acid phosphatase and the establishment of correlations between these parameters and incidence of complications. It was found that in the isolated course of chronic pancreatitis there is a high (R = 0.60) statistically significant (p <0.01) correlation between the levels of osteocalcin and pancreatic elastase-1. A negative statistically significant (p <0.01) mean correlation (R = -0.49) was found between the content of tartrate-resistant acid phosphatase and age of the patients having comorbidity of chronic pancreatitis and hypertension, as well as there is a moderate correlation between the content of tartrate-resistant acid phosphatase and the duration of hypertension, which is statistically significant (R = 0.36, p <0.01). The levels of total bone phosphatase and tartrate-resistant acid phosphatase in the main group exceeded the reference values in 2.5 and 1.9 times respectively (CMU; U = 866.0; p <0.01), while in the comparison group were 2 times (total bone phosphatase) and 1.3 times higher (tartrate-resistant acid phosphatase) times, respectively (CMU; U = 821.0; p <0.01) that enables to diagnose the development of osteopenic conditions. That is, the combined course of chronic pancreatitis and hypertension should be considered as unfavourable tandem in the development of secondary osteoporosis and requires early osteoporotic screening.


2012 ◽  
Vol 11 (6) ◽  
pp. 4-8
Author(s):  
I. S. Golovunina ◽  
F. Yu. Mukharlyamov ◽  
M. A. Rassulova ◽  
E. S. Ivanova

Aim. To assess the effects of the multiple-set resistance exercise on the functional status of patients with Stage II essential arterial hypertension (EAH). Material and methods. In total, 40 patients with Stage II EAH, aged 45-55 years, took part in the physical rehabilitation programme, including exercise training on the resistance exercise machine. In the control group, weight lifting was followed by the complete relaxation of involved muscle groups and, therefore, a fast weight lowering. In the main group, the muscle relaxation after weight lifting was only partial, with a slower weight lowering, which resulted in increased lactate production and muscle ache. Ergospirometry, volumetric compression oscillometry, and resistance tests were used. Results. Blood pressure normalization, myocardial contractility increase, and exercise capacity improvement were more pronounced in the main group (p<0,05), as a greater extent of neuromuscular strain stimulates the hormone production both during and after the exercise session. Conclusion. The complex multiple-set resistance exercise programme demonstrated a marked beneficial effect in patients with Stage II EAH.


2004 ◽  
Vol 57 (3-4) ◽  
pp. 153-158 ◽  
Author(s):  
Jovica Jovanovic ◽  
Milan Jovanovic

Introduction Arterial hypertension is an important medical, social and economic problem in the working population. Factors of occupational exposure lead to a much faster and more frequent occurrence of this disease and its consequential complications. Among these factors the following take an important place: noise, carbon monoxide, job dissatisfaction, microclimate conditions, chemical agents, shift work, night work, monotonous work and so on. The purpose of this study was to analyze working conditions, working environment, work demands and identification of occupational Stressors and their influence on development of arterial hypertension. Material and methods This investigation included 3470 workers and it examined technological processes, working time, job satisfaction, interpersonal relations and physical and chemical hazards of their workplaces. The exposed group consisted of 2270 workers occupationally exposed to Stressors. Control group included 1200 workers whose workplaces were without Stressors. Workers of exposed group were divided into 14 subgroups in regard to presence of occupational hazards. Results Arterial hypertension was established in 39.9% of workers of exposed group, which is statistically significantly more than in controls (25.7%). The highest prevalence of arterial hypertension was at workers who were dissatisfied with the job and who were at the same time occupationally exposed to noise and carbon monoxide. Family predisposition and smoking have cumulative effects with occupational hazards. Discussion Possible mechanisms of arterial hypertension development include activation of adrenergic nervous system, renin-angiotensin aldosteron system, higher concentrations of steroids, catecholamines and free radicals caused by occupational hazards. Conclusion Occupational Stressors are significant factors in development of arterial hypertension of exposed workers. The most important Stressors are noise, carbon monoxide and job dissatisfaction.


2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
K Yushko ◽  
S Koval ◽  
I Snihurska ◽  
O Mysnychenko

Abstract Introduction Blood pressure (BP) elevation leads to chronical increasing left ventricular (LV) overload, LV hypertrophy (LVH), impaired LV relaxation, development of LV diastolic dysfunction (DD), left atrial (LA) enlargement, which result in development of hypertension heart disease (HDD), increased risk of atrial fibrillation and heart failure. MicroRNA-133a is one the epigenetics factor, which has cardioprotective properties, but its role in the development of HHD in hypertension patients with obesity is not well understood. Purpose The aim of the study was to investigate the circulating microRNA-133a levels in patients with hypertension and obesity and to define its associations with components of HHD. Methods The study involved 82 patients with arterial hypertension grades 2–3 and obesity (44 men and 38 women) aged 43 to 70. All patients were separate in two groups: 45 patients with HHD (main group) and 37 patients without HHD (comparison group). The control group consisted of 21 practically healthy individuals of comparable gender and age. The HHD was defined as LVH in hypertension patients with/without LV DD. The LVH was determined according to the 2018 ESC/ESH guidelines. The LV DD was determined according to the 2016 ASE/EACVI recommendations. Obesity was diagnosed at body mass index ≥30 kg/m2. Circulating plasma microRNA-133a levels were obtained by polymerase chain reaction using the CFX96 Touch System, “TaqMan microRNA Assay” and “TaqMan® Universal PCR Master Mix” reagent kits. Results The levels of microRNA-133a in main group were significantly lower than in comparison group and control group (0.133 [0.099; 0.184] vs 0.238 [0.155; 0.410], p&lt;0.05 and 0.382 [0.198; 0.474]), p&lt;0.05). In the main group 23 patients had LV DD and 25 patients had normal LV diastolic function. In patients with LV DD the levels of circulating plasma microRNA-133a were significantly lower than in patients with normal LV diastolic function (0.094 [0.067; 0.147] vs 0.152 [0.092; 0.189]), p&lt;0.05). In the main group there was a statistically significant negative correlation between microRNA-133a levels and LV mass index (R=−0.37, p&lt;0.01) and no significant correlation with ejection fraction, LA size or E/e' (averaged) ratio (p&gt;0.05). In patients of the comparison group there were not significant associations of microRNA-133a levels with LV parameters (p&gt;0.05). Conclusion The results of the study indicate a significant role of decreased circulating plasma microRNA-133a levels in patients with arterial hypertension and obesity in the pathogenesis and development of HHD and such its components as LVH and LV DD. Funding Acknowledgement Type of funding source: None


2020 ◽  
Vol 92 (12) ◽  
pp. 25-30
Author(s):  
S. Yu. Nikulina ◽  
V. A. Shulman ◽  
A. A. Chernova ◽  
S. V. Prokopenko ◽  
D. A. Nikulin ◽  
...  

Aim.To study the association of single-nucleotide polymorphismrs3025058(5а/6а) with the development of stroke in patients of the East Siberian population with cardiovascular pathology and risk factors for its development. Materials and methods.The study involved 260 patients with stroke (age [57.0; 51.062.0]) and 272 patients of the control group (age [55.0; 51.062.0]). Among the patients who underwent stroke, 157 men and 103 women. The control group included 170 men and 102 women. The examination of the main group included: collection of complaints, anamnesis, clinical examination, computed tomography of the brain, electrocardiography, echocardioscopy, ultrasound duplex scanning of the extracranial brachiocephalic arteries, 24-hour monitoring of blood pressure and heart rate, analysis of the blood coagulation system. The patients of the main group had the following cardiovascular pathology and risk factors: arterial hypertension, paroxysmal supraventricular tachycardias, dyslipidemia, atherosclerosis of extracranial brachiocephalic arteries, disorders of the hemostasis system. The control group was examined within the framework of the international project HAPIEE. Molecular genetic research was carried out by real-time PCR. Statistical processing of the material was carried out using the Statistica for Windows 7.0, Excel and SPSS 22 application software. Results.The study established statistically significant associations between the 5a/5a genotype and the 5a allele and stroke in the general group of patients, as well as in the subgroup of men, subgroups of patients with extracranial brachiocephalic arteries atherosclerosis and dyslipidemia. In the subgroup of patients with cardiac arrhythmias, statistically significant results were obtained only for allele 5a, and in the subgroup of women with stroke, subgroups of patients with arterial hypertension and hypercoagulation, no significant associations ofrs3025058(5a/6a) polymorphism with stroke were found. Conclusion.Genotype 5a/5a and allele 5a of the single-nucleotide polymorphismrs3025058(5а/6а) increase the risk of stroke in individuals from the East Siberian population, including those in the presence of such risk factors as extracranial brachiocephalic arteries atherosclerosis and dyslipidemia.


2020 ◽  
pp. 94-94
Author(s):  
D.B. Khamidov ◽  
Z.K. Kosimov ◽  
B.R. Boboev ◽  
U.R. Khalifaev ◽  
Sh.E. Kholnazarov

Objective. Study of the effect of Rheosorbilact on the rheological properties of blood in patients with acute peritonitis. Materials and methods. 62 patients with acute peritonitis at the age from 20 to 87 years were examined. The patients were divided into two groups depending on the inclusion of Rheosorbilact solution in the infusion therapy program. The first group (control) included 30 patients with the infusion therapy program consisted of conventional crystalloid and colloidal infusions. The second (main) group included 32 patients with the treatment of Rheosorbilact infusion therapy program at an average dose of 5.7-6.6 ml/kg (400 ml per day). The rheological properties of blood were studied by determining the relative blood viscosity with a VK-4 viscometer, hematocrit, fibrinogen and ESR according to generally accepted methods. Results. In patients with acute peritonitis, when Rheosorbilact (main group) is included in the infusion therapy program, there is a significant improvement in the rheological properties of blood compared with the results of the control group who received crystalloids and colloids. After treatment in patients of the control group, the indicators of the rheological properties of blood were characterized by a decrease in hematocrit – by 16.5 %, blood viscosity – by 11.6 %, fibrinogen content – by 15.2 %, ESR – by 18.2 % compared to the initial data. In patients of the main group who received Rheosorbilact in the infusion therapy program, the rheological properties of the blood significantly improved and amounted to 24.0 % in comparison with the initial data on hematocrit, 18.7 % in blood viscosity, 21.0 % in fibrinogen, and 23.4 % in ESR. Conclusions. In patients with acute peritonitis, a significant violation of the rheological properties of blood is observed. The inclusion of Rheosorbilact in the infusion therapy program contributes to the correction of impaired blood.


2016 ◽  
Vol 94 (5) ◽  
pp. 374-378
Author(s):  
Anatoliy A. Elgarov ◽  
M. A. Kalmykova ◽  
M. A. Elgarov

Aim: to determine the frequency of respiratory sleep disorders (RSD) in vehicle drivers, peculiarities of their clinical status and workability. Material and methods. The study included the main group of 106 vehicle drivers (VD) and control group of 104 of subjects of other professions. The following methods were used: fibrooptic endoscopy, standard questionnaire, pulsoxymetry, 24 hour blood pressure and ECG monitoring, psychophysiological testing of 86 VD with RSD, arterial hypertension with and without complications. Results. The clinical and instrumental signs of RSD were found in 65.1% VD of the main group and 47,1% of controls. Transitory ischemic attacks, cardiac arrhythmias and their combination occurred more frequently in VD (82.6, 59.2, and 52.2% respectively) than in controls (51.0, 44.8, and 32.6%). In most observations of VD, the smoking rate (98.1%), alcohol intake (95.3%), increased body mass (74.5%), obese (21,7%) dyslypoproteidemia (73.6%), left ventricular hypertrophy (54.7%) and arterial hypertension (57.5%) were significantly higher than in controls : S (76.9%), AI (75.0%), IBM (56.7%), O (18.3%), DLP (59.6%), LVH (41.3%), AH (39.4%). Chronic obstructive pulmonary diseases was diagnosed in 55.6% of VD and 51.9% of controls, diabetes mellitus in 34.9% and 20.2%, epidemiological forms of ischemic heart disease in 22.6% VD and 20.2% controls. Psychophysilogical testing of VD group revealed deterioration of professional qualities and functions of the drivers with respiratory sleep disorders. Conclusion. Significant frequency of RSD among VD (65.1%) in comparison with subjects of other professions (47.1%) was documented. RSD cause deterioration ofprofessional abilities of VD which increases the risk of traffic accidents.


2013 ◽  
Vol 12 (5) ◽  
pp. 56-61 ◽  
Author(s):  
Yu. V. Lukina ◽  
S. Yu. Martsevich ◽  
A. V. Zagrebelnyi ◽  
S. G. Tripkosh ◽  
F. A. Aidu ◽  
...  

Aim.Using the data from the PROFILE Registry and the patients’ questionnaire survey, to analyse the patients’ compliance with medical recommendations on lipid-lowering therapy (LLT).Material and methods.Over the period from May 1st 2011 to December 31st 2011, 274 patients were included in the PROFILE Registry: 82 were referred to a specialised medical centre for the first time (control group, CG); 167 were regularly attending the medical centre (main group A, MGA); and 25 last visited the centre >2 years ago (main group B, MGB). 262 patients completed a questionnaire on the therapy compliance, while 12 refused to participate in the questionnaire survey.Results.According to the results of the questionnaire survey, MGA patients were better informed about the LLT goals (65%) than their CG and MGB peers (35% and 48%, respectively; p<0,0001). The percentage of patients who took statins every day was 87% in MGA vs. 41,5% in CG and 64% in MGB (p=0,002). Statin therapy effectiveness was controlled regularly in MGA: cholesterol (CH) levels were measured every 6 months in 37,5% and every 12 months in 28%. For CG, these figures were 17% and 16%, while for MGB, they were 15% and 40%, respectively (p<0,0001). One-third of CG patients, every fourth MGB patient, and only 13% of MGA patients did not control their CH levels (p<0,0001).Conclusion.The MGA patients who were regularly attending the State Research Centre for Preventive Medicine were the most LLT-compliant: they were well-informed about their disease and therapy goals, were regularly taking prescribed medications (statins), and had better treatment control and the highest LLT effectiveness.


2019 ◽  
Vol 18 (1) ◽  
pp. 73-81 ◽  
Author(s):  
I. Е. Deneka ◽  
A. V. Rodionov ◽  
V. V. Fomin

Aim.To evaluate the effectiveness of telmisartan as a component of triple antihypertensive therapy in patients with obesity and refractory arterial hypertension.Material and methods.The study included 30 patients with obesity and refractory arterial hypertension. All patients received an angiotensin II receptor blocker (ARB) or an angiotensin-converting enzyme inhibitor (ACE inhibitor) in an adequate dosage as part of a triple antihypertensive therapy that also includes a calcium antagonist and thiazide diuretic. Participants were randomly divided into two equal groups. In the main group, telmisartan (Dr. Reddy’s Laboratories) was prescribed at a dosage of 80 mg/day instead of the previously taken ARB or ACE inhibitor. In the control group, patients continued to receive a previously prescribed ARB or an ACE inhibitor. The primary end point was a decrease in mean daily systolic and/or diastolic blood pressure (SBP and DBP) according to 24-hour blood pressure monitoring at 10 mm Hg and more than 20 weeks after the start of the study. The secondary end point was a decrease in the concentration of inflammatory markers and an increase in the level of adiponectin by 15%.Results.After 20 weeks, in the telmisartan group, we noted a significant decrease in the average daily SBP and DBP: in the main group from 145,9±5,4/95,6±4,8 mm Hg to 134,8±3,0/84,9±4,2 mm Hg, in the control group with 147,2±4,9/96,4±5,6 mm Hg to 142,4±4,3/96,9±62 mm Hg. We also determined an increase in the serum adiponectin concentration from 9,3±5,6 μg/ml to 13,4±6,6 μg/ml in the main group and a decrease from 8,8±5,2 μg/ml to 8,6±5,6 μg/ml in the control group, as well as a decrease in the concentration of highly sensitive C-reactive protein (main group from 8,8±3,0 to 6,0±2,8 mg/l and the control group from 7,4±3,8 to 6,9±4,9 mg/l) and interleukin-6 (main group from 16,2±6,1 to 12,3±2,8 pg/ml, control group from 22,5±4,2 to 19,9±5,2 pg/ml).Conclusion.Telmisartan can be considered as a drug of choice as part of multi-component therapy in the treatment of patients with obesity and refractor arterial hypertension.


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