scholarly journals Host range testing of a nucleopolyhedrovirus of the lightbrown apple moth Epiphyas postvittana

2007 ◽  
Vol 60 ◽  
pp. 26-32
Author(s):  
J. Poulton ◽  
N.P. Markwick ◽  
V.K Ward ◽  
V. Young

Epiphyas postvittana nucleopolyhedrovirus (EppoNPV) has considerable potential as a biocontrol agent or biopesticide for control of lightbrown apple moth Epiphyas postvittana a pest of horticultural crops in New Zealand and a market access problem in export fruit To obtain regulatory approval for EppoNPV in either role EppoNPV must not negatively impact on nontarget organisms In this study eight nontarget insect species from one hymenopteran and five lepidopteran families were inoculated with EppoNPV at high titre (109 PIB/ml) Larval survival growth rates pupation and pupal weights were measured and larvae examined for EppoNPV Minor differences in one parameter were found in Helicoverpa armigera and Cydia pomonella but no viral infection Growth and survival were compromised in virusfed individuals in only one species Tyria jacobaeae The majority of T jacobaeae larvae had high microsporidal infections and EppoNPV polyhedra were found in only one larva suggesting a very low likelihood of field infectivity

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Risaldo Tjodi ◽  
Ockstan J Kalesaran ◽  
Juliaan Ch Watung

This study aimed to determine the best combination of feed on the growth and survival of larval fish catfish (Clarias gariepinus). This study  used completely randomized with six treatments including treatment A: Artemia, B: Tubifex C: Pellet, D:  combination of (50%) Artemia + (50%) Tubifex, E:  combination of (50%) Artemia + (50%) Pellet, and      F:  combination of (50%) Tubifex + (50%) Pellet. Each treatment consisted of three replications. The result showed that the combination of  50% artemia + 50% tubifex could promote the growth length of larvae of 4,003 cm, weight of larval of 0.633 g,  and larval survival was 79%.  This combination was the best combination for the growth and survival of catfish larvae.   Keywords: Combination of feed, Clarias gariepinus, larvae, growth, survival


2017 ◽  
Vol 70 ◽  
pp. 322
Author(s):  
R.T. Wilkinson ◽  
L.E. Jamieson ◽  
A.J. Hawthorne ◽  
D.E. Hartnett ◽  
N.E.M. Page-Weir ◽  
...  

Codling moth, Cydia pomonella (Lepidoptera: Tortricidae), is a key pest of apples exported from New Zealand and is dif cult to control at levels required to ensure quarantine security demanded by many countries. Market access for New Zealand apples into countries with strict codling-moth quarantine regulations currently relies on methyl bromide fumigation combined with cold treatment (e.g. Japan) or the use of a rigorous systems approach (e.g. Taiwan). Detection of codling moth in apples would enable the very few apples in the packhouse with codling moth to be graded out. In this study, a commercially available Compac Spectrim grading system was used to determine if codling moth entry holes could be detected. This system provides high-clarity images through enhanced lighting and optics, as well as using various infrared wavelengths to target different defects and machine-learning algorithms to differentiate defects. Apples infested with first- or third-instar codling moth larvae were processed through the Spectrim machine. The system successfully identi ed 100% of apples infested with rst- instar larvae and 96% of apples infested with third-instar larvae. Additionally, damage caused by the two life stages was able to be differentiated.


2016 ◽  
Author(s):  
A. L. Knight ◽  
E. Basoalto ◽  
G. J. R. Judd ◽  
R. Hilton ◽  
D. M. Suckling ◽  
...  

ABSTRACTA recent discovery have demonstrated that herbivore induced plant volatile compounds from apple tree infested with leafrollers were highly attractive to con-specific adult male and female leafrollers. However, this work has been conducted in New Zealand and Canada testing only low doses of kairomone. This study has been conducted in US to assess the attractiveness of higher doses of the six apple volatiles provisory identified in apple trees infested by tortricid larvaeto the leafroller, Pandemis pyrusana Kearfott. These volatiles included, β-caryophyllene, germacrene D, benzyl alcohol, phenylacetonitrile, (E)-nerolidol, and indole. No volatiles were attractive to P. pyrusana when used alone. However, traps baited with phenylacetonitrile plus acetic acid caught both sexes of P. pyrusana. Traps baited with the other volatiles plus acetic acid caught zero to only incidental numbers of moths, ≤ 1.0. Adding phenylacetonitrile to traps baited with pear ester, ethyl (E,Z)-2,4-decadienoate plus acetic acid significantly reduced catches of codling moth, Cydia pomonella (L.). However, adding phenylacetonitrile to traps baited with codling moth sex pheromone, pear ester, and acetic acid did not similarly reduce moth catches of C. pomonella. Interestingly, traps baited with phenylacetonitrile plus acetic acid caught significantly more P. pyrusana than traps baited with a commercial sex pheromone lure. The evaporation rate of the acetic acid co-lure was an important factor affecting catches of P. pyrusana with phenylacetonitrile, and studies are needed to optimize the emission rates of both lure components. Further studies are warranted to develop phenylacetonitrile and possibly other aromatic plant volatiles as bisexual lures for the range of tortricid pests attacking horticultural crops.


2001 ◽  
Vol 54 ◽  
pp. 33-36
Author(s):  
P.W. Shaw ◽  
P. Lo ◽  
D.R. Wallis

To assist the natural distribution of the leafroller parasitoid Trigonospila brevifacies to horticultural areas in the Nelson region 181 T brevifacies pupae and 329 adults were released in 1999 at four sites adjacent to apple orchards near Motueka Release material was supplied from fieldcollected parasitoids in Hawkes Bay and a laboratory colony held at the Mt Albert Research Centre in Auckland Successful establishment of the parasitoid was recorded at all four sites and at a nearby nonrelease site in 2000 In 2000 319 of fourth instar and older larvae were parasitised by T brevifacies while in 2001 parasitism was 1166 Important pest tortricid species including Epiphyas postvittana (Walker) (lightbrown apple moth) were among the hosts The successful establishment of T brevifacies in Nelson and its further natural dispersal will increase the potential for the reduction of economically important leafroller species by biocontrol agents


2015 ◽  
Vol 68 ◽  
pp. 193-196
Author(s):  
P.W. Shaw ◽  
D.R. Wallis

Leafroller larvae and pupae were collected during timed searches in January and April 2013 from host plants at six scrub vegetation sites in two Nelson horticultural districts approximately 40 km apart The numbers of leafrollers and parasitoid species recovered were similar in both districts Most of the leafroller larvae collected were Epiphyas postvittana (Walker) (lightbrown apple moth LBAM) and most of these were found on gorse and broom In total 293 larvae and 11 pupae were reared successfully with 39 parasitism A wide diversity of parasitoids was recovered comprising 10 species from five families with Dolichogenidea tasmanica (Braconidae) the most common (80) The diversity of parasitoids and parasitism of leafrollers in unmanaged environments outside orchards may help to reduce leafroller pressure and the risk of fruit infestation in surrounding commercial orchards


1999 ◽  
Vol 39 (3) ◽  
pp. 335 ◽  
Author(s):  
C. Chervin ◽  
S. L. Kreidl ◽  
P. R. Franz ◽  
A. J. Hamilton ◽  
S. R. Whitmore ◽  
...  

Australian pome fruit exports to northern America and Japan are rejected if they contain lightbrown apple moth (Epiphyas postvittana Walker) or codling moth (Cydia pomonella Linnaeus) respectively. A warm controlled atmosphere (CA) disinfestation treatment (72 h at 28˚C at 0, 1 or 2 kPa O2 ), followed by varying lengths of cold storage (5 weeks, 10 weeks or 6 months), is described. Four cultivars were studied: Packham’s Triumph pears (Pyrus communis Linnaeus); Royal Gala; Fuji and Pink Lady apples (Malus domestica Borkh.). Consumer panels found that fruit subjected to the 2 kPa O2 warm CA treatment and cold storage were as acceptable as control fruit. This is an important finding as previously published results for a harsher treatment described treated fruit (especially Pink Lady) as being less acceptable than controls. Some advanced ripening was observed for treated Packham’s Triumph pears. After 5 and 10 weeks cold storage, treated Royal Gala apples were generally firmer than the controls. Insects were also subjected to the 2 kPa O2 warm CA treatment. The most tolerant life stage (MTS) of lightbrown apple moth was the sixth instar with an LT95 of 37 days cold storage (0.5˚C) after the 2 kPa O2 warm CA treatment. Comparison with previous research suggests that the 2 kPa O2 warm CA treatment approximately halves the time required in cold storage for effective control of late instar lightbrown apple moth. A LT95 of 81 days was achieved for fifth instar codling moths (considered to be the MTS) and this may need to be reduced for export to Japan. Preliminary observations suggested that there may not be substantial difference between the resistance of non-diapausing and pre-diapausing codling moth larvae to the warm CA followed by cold treatment.


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