scholarly journals Antibiotic sensitivity of Pseudomonas aeruginosa strains isolated from milk of highly productive dairy cows

2021 ◽  
Author(s):  
O.A. Artemyeva
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Research Work ◽  
Antibiotic Sensitivity ◽  
Biochemical Properties ◽  
Sensitivity Testing ◽  
Black And White ◽  
Milk Samples ◽  
Living Bacterium ◽  
Clinically Significant ◽  
Phenotypic Methods

Pseudomonas aeruginosa is a free-living bacterium that is conditionally pathogenic in natural conditions, but when ingested by an animal can cause severe infectious diseases, depending on the location and stage of infection, increasing the time of convalescence. It is naturally resistant to a number of widely used antibacterial drugs: fluoroquinolones, tetracyclines, chloramphenicol; it is capable of forming a biofilm. The aim of this work was to assess the level of sensitivity of strains isolated from cow's milk to the main groups of antibiotics with clinically significant anti-pseudomonasal activities. The work provides microbiological monitoring data for 2020-2021. During this period, 350 milk samples were taken from Holstinized black-and-white cows of the dairy direction of various lactation ages. All isolates were characterized by generally accepted phenotypic methods, with confirmation of biochemical properties. A number of antibiotics selected in this work comply with the recommendations of EUCAST. Sensitivity testing of the isolated strains was carried out by the disco-diffuse method. Pseudomonas aeruginosa was detected in 69 milk samples, which accounted for 19.7% of the total number of milk samples. Pseudomonas Aeruginosa was isolated as a monoculture in 42.03% of cases, in association with gram (-) bacteria in 20.29% and gram (+) in 37.68%. The percentage of pigmented strains in our work was 98.55% of all isolated strains, and 1.45% of poorly pigmented. During the research work, it was found that more than 90% of the strains were resistant to cefepime, the rest showed partial resistance. Therefore, it can be recommended for use only after a correction for sensitivity to this drug. The aminoglycoside group drugs, amikacin and gentamicin, had the highest activity - over 90% against the isolated Ps.a. strains. The results obtained indicate that the isolated strains of Ps.a. they showed high resistance to representatives of the cephalosporin group, which is also increasing with respect to fluoroquinolones, which is a serious problem in the spread of antibiotic resistance.

scholarly journals ISOLATION AND IDENTIFICATION OF MICROFLORA FROM APPARENTLY HEALTHY CAGED PARROTS OF DHAKA ZOO OF BANGLADESH

1970 ◽  
Vol 8 (1) ◽  
pp. 05-10 ◽  
Author(s):  
J Akhter ◽  
MT Hossain ◽  
MT Islam ◽  
MP Siddique ◽  
MA Islam
Keyword(s):  
Research Work ◽  
Antibiotic Sensitivity ◽  
Biochemical Properties ◽  
Gram Negative Bacteria ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Gram Negative ◽  
E Coli ◽  
Apparently Healthy

The research work was conducted to isolate and identify the microflora from apparently healthy caged parrots. A total of 45 samples (oral swabs, cloacal swabs and feces) were collected from five types of caged parrots (Gray cockatiels, Rose ringed parakeet, Alexandriane parakeet, Red breast parakeet and Blossom headed parakeet) of Dhaka Zoo during the period from April to August 2009. The samples were cultured on different bacteriological media and the bacteria were identified by their cultural and biochemical properties. All the isolates were allowed for antibiogram study. The bacteria isolated in this study from different types of caged parrots were E. coli (64.44%), Salmonella spp. (46.67%), Staphylococcus spp. (46.67%), Pasteurella spp. (33.33%), Proteus spp. (6.67%) and some unidentified Gram-positive and Gram-negative bacteria. Of these isolates, E. coli was the most frequent isolate. The frequency of Gram-negative bacteria was higher in this study. The percentage of bacterial isolates recovered from each type of parrots was almost similar. Irrespective of types of parrots, the higher percentage of different bacteria was isolated from cloacal swab (77.78%) followed by feces (75.56%). The 68.89% isolates were recovered from oral swab. All the suspected isolates of Salmonella spp. were confirmed by slide agglutination test using Salmonella polyvalent ‘O’ antiserum. Among the 21 Salmonella spp. isolated in this study, 4 (19.05%) isolates were identified as S. Pullorum when tested with specific antisera against S. Pullorum. The results of antibiotic sensitivity tests revealed that ampicillin and amoxicillin were completely resistant to E. coli and Pasteurella spp.; ampicillin to Proteus spp.; and furazolidone to Salmonella spp. and Pasteurella spp. However, the antibiotics of fluoroquinolone group such as ciprofloxacin, norfloxacin and enrofloxacin showed moderate to high sensitivity against almost all the bacterial isolates. Of these, ciprofloxacin was found to be consistently highly sensitive to all the bacterial isolates. DOI = 10.3329/bjvm.v8i1.8349 Bangl. J. Vet. Med. (2010). 8(1): 05-10

scholarly journals Correlation of biofilm production with antibiotic susceptibility pattern of Pseudomonas aeruginosa from various clinical specimens

2022 ◽  
Vol 13 (1) ◽  
pp. 88-92
Author(s):  
M Swapna ◽  
G Sumathi ◽  
M Anitha
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Biofilm Formation ◽  
Antibiotic Sensitivity ◽  
Microtiter Plate ◽  
Resistance Pattern ◽  
Sensitivity Testing ◽  
Clinical Specimens ◽  
Biofilm Production ◽  
Microbiological Methods

Background: Pseudomonas aeruginosa is one of the most prevalent nosocomial pathogens that cause a life-threatening infection. One of the important characteristics of P. aeruginosa is biofilm formation which leads to antibiotic resistance. Aims and Objectives: The aim of the study was to study the antibiotic resistance pattern of P. aeruginosa isolates and correlation with their biofilm-production. Materials and Methods: A total of 87 P. aeruginosa isolates from different clinical specimens were processed and confirmed by conventional microbiological methods as per standard methodology. Antibiotic sensitivity testing was done for all isolates. Biofilm producing isolates were identified by the microtiter plate method (MTPM). Results: Of 87 P. aeruginosa isolates, majority were from pus 33 (38%), followed by urine 26 (30%), sputum 19 (22%), body fluids 7 (8%), and blood 2 (2%). Biofilm producing isolates showed more resistance in comparison to non-biofilm producers. The observed difference between biofilm formation for multidrug resistant and susceptible isolates was found to be statistically significant. Conclusion: MTPM method was an effective test for detection of biofilm formation and was also able to verify biofilm production by P. aeruginosa. This indicated a higher propensity among the clinical isolates of P. aeruginosa to form biofilm and revealed a positive correlation between biofilm formation and antibiotic resistance. This indicates the need for testing of even susceptible isolates for virulence factors such as biofilm production.

scholarly journals Pandoraea apista Bacteremia in a COVID-Positive Man: A Rare Coinfection Case Report from North India

2021 ◽  
Author(s):  
Sweta Singh ◽  
Chinmoy Sahu ◽  
Sangram Singh Patel ◽  
Atul Garg ◽  
Ujjala Ghoshal
Keyword(s):  
Unusual Case ◽  
Research Work ◽  
Clinical Manifestations ◽  
Antibiotic Sensitivity ◽  
Multidrug Resistant ◽  
North India ◽  
Sensitivity Testing ◽  
Negative Bacillus ◽  
Automated Methods ◽  
Respiratory Specimens

Abstract Pandoraea apista is a novel gram-negative bacillus usually isolated from respiratory specimens of cystic fibrosis patients. Few cases of bacteremia have also been reported due to this rare pathogen. Emergence of multidrug-resistant isolates of this bacillus is of grave concern. Here, we report a very interesting and unusual case of Pandoraea apista bacteremia in a coronavirus disease (COVID)–positive elderly diabetic man suffering from pneumonia. Prompt isolation and antibiotic sensitivity testing guided the patient’s treatment and yielded favorable outcome. The need of automated methods for identification and sensitivity testing limits the reporting of this rare but important pathogen in hospital settings. Detailed research work and studies are needed in this direction to better understand this pathogen and its clinical manifestations for better patient outcome.

scholarly journals Evaluation of blaGES-5 and bla veb-1 genes with multidrug-resistant extend, pandrug resistance patterns (MDR, XDR, PDR), and biofilm formation in Pseudomonas aeruginosa isolates

2021 ◽  
Vol 67 (3) ◽  
pp. 52-60
Author(s):  
Fattma A. Ali ◽  
Sevan Hassan Bakir ◽  
Sayran Hamed Haji ◽  
Bashdar M. Hussen
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Phylogenetic Trees ◽  
Antibiotic Sensitivity ◽  
Multidrug Resistant ◽  
Clinical Samples ◽  
Sensitivity Testing ◽  
Automated Method ◽  
Resistance Patterns ◽  
Vitek 2

Pseudomonas aeruginosa is a ubiquitous microorganism that is difficult to treat due to the increasing prevalence of multidrug resistance patterns. A total of 227 samples were taken from different clinical samples during the study period from January 2018 to December 2018. The isolates were identified with antibiotic sensitivity testing with ESBL by the Vitek-2 automated method. MDR, XDR, and PDR were determined. 40 (17.6%) isolates were positive for P. aeruginosa, maximum of ESBL and MBL. Positive isolates were detected in the burn, coexisting ESBL + MBL enzymes in 21 (52.5%) of our isolates. Imipenem followed by Meropenem were found to be effective against ESBL and MBL producers. Resistance was reached between 72-100% to 5 antibiotics. The frequency of PDR, MDR, and XDR were 5%, 50%, and 45%, respectively. The frequency of co-production between MDR, XDR, and PDR with MBL, ESBL, and Biofilm was 35%, 12.5% and 5%, respectively. Among the ESBLs, the frequency of distribution of bla VEB-1gene and blaGES-5 gene was 50% and 40 %, respectively. Bacterial isolates simultaneously carrying blaVEB-1 gene with multiple ?-lactamases of different classes of biofilm, MDR, PDR, and XDR as same as a coexisting blaGES-5 gene. One isolate was detected as new isolates registered in global gene bank as locally P. aeruginosa isolates in Erbil city (LOCUS MN900953). The phylogenetic trees of the blaVEB gene isolates were demonstrated a genotype closely related to others, deposited in GenBank similar to the P. aeruginosa gene; gene sequencing revealed a 99% similarity with other isolates deposited in GenBank.

Multidrug-resistant Pseudomonas aeruginosa isolated from ear and wound swabs in some selected hospital laboratories in Sokoto Metropolis, Nigeria

2020 ◽  
Vol 3 ◽  
pp. 31-35
Author(s):  
A. I. Umar ◽  
I. Garba ◽  
M. L. Jidda ◽  
A. M. Ganau ◽  
A. S. Fana ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Agents ◽  
Opportunistic Infections ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Sensitivity Testing ◽  
Wide Range

Objective: Pseudomonas aeruginosa is a metabolically versatile bacterium that can cause a wide range of severe opportunistic infections in patients with serious underlying conditions and can be found in most communities in Nigeria. The study was to determine the prevalence, resistance pattern and distribution of multiple drug resistant P. aeruginosa (MDR-PA) isolated from ear and wound specimens in patients attending Specialist Hospital Sokoto and Maryam Abacha Women and Children Hospital Sokoto. Materials and Methods: A total of 150 samples were analysed by standard bacteriological methods. Screening for MDR-PA was carried out by antibiotic sensitivity testing using disc diffusion method with ceftazidime (30 µg), ofloxacin (5 µg), cefuroxime (30 µg), cloxacillin (30 µg), amoxicillin (30 µg), ceftriaxone (30 µg), imipenem (10 µg), gentamycin (10 µg) and colistin (10 µg) discs on Mueller Hinton agar. Results: Of a total of 55 (36.7%) isolates of P. aeruginosa strains were obtained, 30 (54.5%) isolates were resistant to imipenem, 31 (56.3%) were resistant to ofloxacin, 44 (80.0%) to gentamycin, 53 (96.3%) to ceftazidime and cefuroxime, 50 (90.9%) to ceftrizone, 54 (98.1%) to cloxacillin and amoxycillin and lastly 15 (27.2%) to colistin. All the isolates were multi drug resistant, this probably due to improper use or over the counter purchase of antibiotics without professional oversight. Conclusion: Almost all the isolates were resistant to all the antibiotics used including colistin. There is need to improve on the public health awareness on the indiscriminate use of antimicrobial agents as it is one of the major ways microbes develop resistance to them.

scholarly journals Bacteriological Profile Of Ear Discharge And Their Antibiotic Sensitivity In Chronic Suppurative Otitis Media In Kashmir,India

2012 ◽  
Vol 11 (3) ◽  
pp. 212-216 ◽  
Author(s):  
A Yousuf ◽  
M Malik ◽  
IU Shamas ◽  
Z Beigh ◽  
S Kumari ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Otitis Media ◽  
Medical Science ◽  
Antibiotic Sensitivity ◽  
Chronic Suppurative Otitis Media ◽  
Diffusion Method ◽  
Sensitivity Testing ◽  
Disk Diffusion Method ◽  
Suppurative Otitis Media ◽  
Staph Aureus

The objective of the study was to study the microflora and the antibiograms of patients with chronic suppurative otitis media (CSOM) in Kashmir,in order to provide a guidelines for making a protocol for empirical antibiotic therapy. Study design:Prospective study Material and methods: This study was conducted on OPD basis in Department of Ear, Nose and Throat and HNS Government medical college Srinagar kashmir from july 2010 to june 1011 and one hundred twenty five(125) patients with unilateral or bilateral active chronic suppurative otitis media were prospectively studied.They had chronic ear discharge and had not received antibiotics for the previous five days. Swabs were taken from discharging ear's and sent to microbiological section of hospital were culture for bacteria was done Antibiotic sensitivity testing was done with standard antibiotic discs using Kirby-bauer disk diffusion method.4 Results:From the Clinical specimens off the 125 patients microbiological culture was positive in 110 specimens. The most common causal organisms isolated were Staphylococcus aureus (48%) including 8% of methcillin resistant staph aureus and Pseudomonas aeruginosa (16%) followed by coagulase negative.Antibiotic sensitivities of staph aureus (other than MRSA) showed that 72% isolates were sensitivity to gentamicin,55% to ciprofloxacin followed by 36% to cephalosporins (cetazidime,ceftriaxone) .For pseudomonas aeruginosa 72% isolates were sensitive to piperacillin/tazobactum 48% to ciprofloxacin and 32% to ceftazidime/ceftriaxone. Of the three antibiotics commonly available as topical eardrops,ciprofloxacin and gentamicin has the highest susceptibility rate. DOI: http://dx.doi.org/10.3329/bjms.v11i3.11731 Bangladesh Journal of Medical Science Vol. 11 No. 03 July’12

scholarly journals Assessment of the Physicochemical and Bacteriological Quality of Public Swimming Pool in Selected Hotels in Ado-Ekiti, Nigeria

2019 ◽  
pp. 1-9 ◽  
Author(s):  
Olajumoke Evelyn Onifade ◽  
Busayo Mutiat Olowe ◽  
John Obasanmi
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Enterococcus Faecalis ◽  
Water Samples ◽  
Antibiotic Sensitivity ◽  
Sensitivity Testing ◽  
Pool Water ◽  
Recreational Facility ◽  
Standard Procedures ◽  
Before And After

The physicochemical and bacteriological assessment of pool water samples from selected hotels in Ado-Ekiti, Nigeria was investigated. A total of 10 pool water samples were collected from five pools before and after use following standard procedures. Physicochemical and bacteriological analyses were performed using standard methods. Antibiotic sensitivity testing was carried out according to CLSI procedures. Physicochemical analyses showed that the pool water samples were clear and colourless. The different parameters examined for each of the hotel showed varying degree of values. On the average, it was observed that values for turbidity, temperature and total dissolved solids were above the WHO and EPA permissible limit after use. The total bacterial, coliform and Escherichia coli counts of the pool water samples were higher after use than before use. A total of 21 isolates were recovered and presumptively identified as Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa. From among the 21 isolates, 14 (66.7%) were Escherichia coli, 5 (23.8%) were Enterococcus faecalis while only 2 (9.5%) were Pseudomonas aeruginosa. All the isolates tested for antibiotic sensitivity showed considerable resistance to some of the antibiotics used. Findings from this study showed that some of the pools sampled did not meet up to WHO and EPA standards. Hence, the need for an effective and urgent intervention in constant monitoring of recreational facility to safeguard the health of the pool users.

Determination of Some Heavy Metals in Milk of Cow Grazing At Selected Areas, of Kaduna Metropolis

2017 ◽  
Vol 7 (7) ◽  
pp. 341 ◽  
Author(s):  
Mahmud Mohammed Imam ◽  
Zahra Muhammad ◽  
Amina Zakari
Keyword(s):  
Heavy Metals ◽  
Cadmium Toxicity ◽  
Research Work ◽  
The Body ◽  
Cow Milk ◽  
Scientific Model ◽  
Potential Health ◽  
Milk Samples ◽  
Lead And Cadmium ◽  
Digestion Technique

In this research work the concentration of zinc, copper, lead, chromium, cadmium, and nickel in cow milk samples obtained from four different grazing areas   (kakuri, kudendan, malali, kawo) of Kaduna metropolis. The samples were digested by wet digestion technique .The trace element were determined using bulk scientific model VPG 210 model  Atomic Absorption Spectrophotometer (AAS).. The concentration of the determined heavy metal were The result revealed that Cr,  Ni and Cd were not detected in milk samples from Kawo, Malali  and Kudendan whereas lead (Pb) is detected in all samples and found to be above  the stipulated limits of recommended dietary allowance (NRC,1989) given as 0.02mg/day. Cu and Zn are essential elements needed by the body for proper metabolism and as such their deficiency or excess is very dangerous for human health. However, they were found in all samples and are within the recommended limits while Cd (2.13 – 3.15 mg/kg) in milk samples from Kakuri was found to be above such limit (0.5mg/day). Cow milk samples analyzed for heavy metals in this research work pose a threat of lead and cadmium toxicity due to their exposure to direct sources of air, water and plants in these grazing areas, thereby, resulting to a potential health risk to the consumers.

The improvement of the type Karatomar of Black-and-White cattle breed under the conditions of Northern Kazakhstan

2020 ◽  
pp. 23-31
Author(s):  
L. Ovchinnikova ◽  
E. Babich
Keyword(s):  
Research Work ◽  
Cattle Breed ◽  
Reproductive Traits ◽  
Farm Animals ◽  
Development Indicators ◽  
Black And White ◽  
Breed Type ◽  
Northern Kazakhstan ◽  
White Breed ◽  
White Cattle

In the Republic of Kazakhstan over the past decade positive changes have been observed in increasing the number of farm animals, increasing their productivity, and increasing the gross production of milk, meat and other products. At the same time the development of dairy cattle breeding is mainly due to the breeding of Black-and-White, Red Steppe, Simmental breeds and their intra-breed types as well as imported from abroad Holstein breed. The purpose of the research was to analyze ways to improve the intra-breed type Karatomar of Black-and-White breed in Northern Kazakhstan. Research work has been carried out on breeding animals such as Karatomar type cattle of Black-and-White breed in LLP “Experimental farm Zarechnoe” in the Kostanay district in the Kostanay region. The dynamics of growth and development indicators, reproductive traits of heifers of a new intra-breed type Karatomar of Black-and-White cattle have been studied. A comprehensive assessment of their economically useful traits in the first lactation, determined by the resistance of animals to climatic and forage conditions, the efficiency of breeding of animals of different genotypes has been conducted. It has been found that the replacement heifers – daughters of American breeding sires inherited precocity and showed higher growth energy after 6 months of age and continued to grow rapidly until 18 months. The advantage in metabolism was in the daughters of Holstein sires of the American selection Orbit 4078, and the increased immune status of the organism in the daughters of the sire of the domestic selection Yamal 975 as the most adapted to the conditions of Northern Kazakhstan. The best ability to increasing the milk yield, stable lactation activity, high milk productivity, gross content of dry matter and nonfat milk solids have been characterized by the daughters of sires of the American selection Orbit 4078 and Lowrider 4129. Analysis of economic indicators of milk production has been shown that from cows – daughters of sires Orbit 4078 and Lowrider 4129 for 305 days of lactation received 15,8 and 15,2 % more milk of base fat, the expenditures of EFU per 1 kg of production was lower by 10,2 and 5,9 %. A higher level of profitability has been observed in the group of daughters of the sires Orbit 4078 – 35,5 % and Lowrider 4129 – 33,1 %, which was 8,5 and 10,8 % higher than in the group of daughters of the sire Yamal 975.

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