scholarly journals Therapeutic effect of fenugreek (Trigonella foenum-graecum) seeds extract against arsenic induced toxicity in Charles Foster rats

2021 ◽  
Vol 13 (3) ◽  
pp. 1083-1093
Author(s):  
Vikas Kumar ◽  
Vivek Akhouri ◽  
Sushil Kumar Singh ◽  
Arun Kumar
Keyword(s):  
Body Weight ◽  
Sodium Arsenite ◽  
Arsenic Concentration ◽  
Treated Group ◽  
Fenugreek Seeds ◽  
Trigonella Foenum Graecum ◽  
Arsenic Accumulation ◽  
Ameliorative Effect ◽  
Liver And Kidney ◽  
Trigonella Foenum Graecum L

The prime objective of the present study was to establish fenugreek (Trigonella foenum-graecum L.) seeds extract as an antidote against arsenic induced hepato-renal toxicity in rats. The male Charles Foster rats (weighing 160-180 g) were selected to make arsenic intoxicated model. The arsenic treated group of rats were orally treated with sodium arsenite at the dose of 8 mg/kg body weight/day for 90 days. Thereafter, the arsenic pretreated rats were further administered with fenugreek ethanolic seeds extract at the dose of 250 mg/kg body weight/day for 90 days.  After the completion of the treatment, animals of all the groups were sacrificed for the biochemical and histopathological estimation. The arsenic treated rats showed significant (p < 0.0001) alterations at the various hepatic and renal biomarker parameters and at serum MDA levels in comparison to the control rats.  Significant (p < 0.0001) arsenic accumulation was also observed in the blood, liver and kidney tissues of the arsenic treated rats. However, after the administration with fenugreek seeds extract, significant (p < 0.0001) restoration was observed in the liver and kidney biomarker parameters and at haematological variables. Fenugreek seeds extract administration also significantly (p < 0.0001) reduced the serum MDA levels and arsenic concentration levels in blood, liver and kidney tissues, along with considerable restorations at the cellular architecture of liver and kidney tissues. The study concluded that fenugreek seeds possessed potential hepato-renal ameliorative effect against sodium arsenite induced toxicity in rats, and can be used for its therapeutic value against arsenic poisoning.

scholarly journals Histological changes of uterus after comparative treatment of female rats by Trigonella foenum-graecum seeds and combined oral contraceptive pills

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Ahmed Kaid Naji Allow ◽  
Ayah Rebhi Hilles ◽  
Zainab Yousef ◽  
Norbaiyah Mohamed Bakrim ◽  
Belqees Ahmed ◽  
...  
Keyword(s):  
Body Weight ◽  
Oral Contraceptive ◽  
Treated Group ◽  
Sprague Dawley ◽  
Histological Changes ◽  
Fenugreek Seeds ◽  
Oral Contraceptive Pills ◽  
Trigonella Foenum Graecum ◽  
Combined Oral Contraceptive ◽  
Contraceptive Pills

Introduction: This study evaluates the histological changes of uterine tissue in the female Sprague Dawley rats after administration of Trigonella foenum-graecum (fenugreek) seeds in comparison to combined oral contraceptive pills (COCPs). Methods: Twenty four female Sprague Dawley strain rats of 8 weeks old were divided into A, B, and C groups. Group A was the control and B was administrated with 0.05 mg/kg body weight of COCPs for 15 days. Group C was treated with 750 mg/kg body weight of fenugreek seed aqueous (FSA) extract for 15 days. The uterine tissues were isolated and studied histologically. Results: The uterine weight in the FSA extract treated group was normal, while in the COCPs treated group it was insignificantly increased. The endometrial thickness and endometrial gland density were normal in the FSA extract treated group, while in the COCPs treated group it was atrophied. Decidual-like cells were observed in the COCPs treated group only. Conclusions: Endometrial atrophy and decidual-like cells were noted after COCPs treatment while in the FSA extract and control groups they were absent. The findings suggest that fenugreek seeds could be used as an alternative natural and safe contraceptive product.

scholarly journals EFFECT OF SPINACH AGAINST ARSENIC TOXICITY IN RATS

1970 ◽  
Vol 7 (2) ◽  
pp. 358-363 ◽  
Author(s):  
MZ Islam ◽  
MA Awal ◽  
M Mostofa ◽  
A Ghosh ◽  
A Khair
Keyword(s):  
Body Weight ◽  
Serum Creatinine ◽  
Sodium Arsenite ◽  
Hydride Generation ◽  
Body Burden ◽  
Treated Group ◽  
Liver And Kidney ◽  
Serum Glutamate Pyruvate Transaminase ◽  
T1 And T2 ◽  
Serum Glutamate

The present study was undertaken with a view to evaluate the efficacy of spinach against arsenic (As) induced toxicity in rats during the period between July to October 2008. Thirty six female Long Evans rats (age about 120days; average body weight at day 0 = 154.5g) were randomly divided into three equal groups (n=12) and marked as T0, T1 and T2 groups. Rats of T0 group were given normal feed and water and kept as control. Rats of T1 and T2 groups were given 5mg Sodium arsenite/kg body weight (BW) and 5mg Sodium arsenite/kg (BW) plus spinach extract 100 mg/kg body weight respectively daily for 30 days orally. Four rats from each group were sacrificed at 10 days interval in order to quantitatively determine the As content in liver, lungs and kidney by using Hydride Generation Atomic Absorption Spectrophotometer. Serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and serum creatinine were determined by Autoanalyser. No visible clinical sign were observed in any group of experimental rats except loss of body weight in the spinach treated group. Tissue (lung, liver and kidney) concentration of As was significantly (p<0.01) higher in T1 group rats compared to that of T0 and T2 groups and the highest concentration of As was found in kidney followed by lung and liver in T1 group rats. After 30 days of feeding, spinach significantly (p<0.01) decreased As from lung, liver and kidney. As intoxication significantly (p<0.01) increased SGOT values but insignificantly decrease SGPT values and spinach treatment improve these condition. There was no significant effect found in serum creatinine level.  It can be concluded that feeding of spinach could reduce body burden of As in rats.

Amelioration of oxidative stress by dietary fenugreek (Trigonella foenum-graecum L.) seeds is potentiated by onion (Allium cepa L.) in streptozotocin-induced diabetic rats

2017 ◽  
Vol 42 (8) ◽  
pp. 816-828 ◽  
Author(s):  
Seetur R. Pradeep ◽  
Krishnapura Srinivasan
Keyword(s):  
Oxidative Stress ◽  
Beneficial Effect ◽  
Allium Cepa ◽  
Diabetic Rats ◽  
Dietary Interventions ◽  
Fenugreek Seeds ◽  
Soluble Fibre ◽  
Trigonella Foenum Graecum ◽  
Allium Cepa L ◽  
Trigonella Foenum Graecum L

Oxidative stress has a crucial role in the progression of diabetes and its complications. Soluble fibre-rich fenugreek seeds (Trigonella foenum-graecum L.) are understood to have a beneficial effect in the management of diabetes. Previously, we have shown that the amelioration of diabetic hyperglycemia and related metabolic abnormalities was potentiated by onion (Allium cepa L.) in experimental rats. The present study evaluated the additive beneficial effect of dietary fenugreek seeds (10%) and onion (3%) on oxidative stress in diabetic rats. These dietary interventions lowered oxidative stress, the combination producing a higher beneficial effect (p < 0.05), although not additive. Dietary fenugreek, onion, or fenugreek+onion countered hypercholesterolemia (p < 0.05), especially from low-density lipoprotein−associated fraction by 43%, 35%, and 54%, respectively. Elevated concentrations of cholesterol and triglycerides in the liver and heart under diabetic conditions were significantly counteracted by these dietary interventions, with the additive combination resulting in greater effect. These findings were also corroborated by restoration of histopathological abnormalities of heart and liver tissues along with lowered heart and liver weights. It is inferred that an alleviation of oxidative stress contributes further to the antidiabetic influence and this nutraceutical potential of fenugreek seeds and onion was higher when consumed together.

scholarly journals TRIGONELLA FOENUM-GRAECUM L. EXHIBITS ESTROGENIC EFFECT THROUGH PRESENILIN 2 GENE EXPRESSION IN THE BREAST CANCER CELL LINE MCF-7

2019 ◽  
Vol 12 (1) ◽  
pp. 438
Author(s):  
Kurnia Agustini ◽  
Michael Wink ◽  
Wahono Sumaryono ◽  
Frans Suyatna ◽  
Nurjati Chairani Siregar
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cancer Cell Line ◽  
Active Fraction ◽  
Rt Pcr ◽  
Fenugreek Seeds ◽  
Trigonella Foenum Graecum ◽  
Presenilin 2 ◽  
Trigonella Foenum Graecum L ◽  
Mcf 7

Objective: The objective of this study is to investigate the estrogenic and antiestrogenic activity of Fenugreek seeds, Trigonella foenum-graecum L. in the estrogen-dependent breast cancer cell line, MCF-7, including its effect on the expression of estrogen-dependent presenilin 2 (pS2) gene.Methods: An activity guided fractionation was carried out with extracts from fenugreek seeds in MCF-7 cells. Cytotoxic activity assays were conducted with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. Most fractions were also tested also tested in media with estradiol 10 nM We also analysed the expression of pS2 gene. For the analysis of pS2 gene expression we employed PCR primers for pS2 and for β-actin as a housekeeping gene using real-time polymerase chain reaction (RT-PCR).Results: Based on cytotoxic activity assay in MCF-7, the active fractions are ethyl acetic fraction and its phases ethyl acetic (EA) 2 and EA 2.2. The most active fraction was EA 2.2 (IC50=27.129 ppm), which exhibited a biphasic effect; at low concentrations, it stimulated the growth, and at high concentrations it showed strong cytotoxic effects. EA2.2 fraction in concentration 20 ppm, also could induce pS2 gene expression in media with and without estrogen.Conclusion: The most active fraction was the ethyl acetate phase and further subfractions. The most active fraction also induced the expression of pS2 gene which was studied by RT-PCR.

scholarly journals Hepatoprotective and nephroprotective effects of Trigonella foenum-graecum L. (Fenugreek) seed extract against sodium nitrite toxicity in rats

2019 ◽  
Vol 6 (5) ◽  
pp. 3142-3150 ◽  
Author(s):  
Gözde ATİLA USLU ◽  
Hamit USLU ◽  
Yasemen ADALI
Keyword(s):  
Sodium Nitrite ◽  
Feeding Habits ◽  
Interleukin 1 ◽  
Food Additives ◽  
Physiological Saline ◽  
Seed Extract ◽  
Protective Effects ◽  
Trigonella Foenum Graecum ◽  
Liver And Kidney ◽  
Trigonella Foenum Graecum L

Introduction: Feeding habits and environmental factors may rival genetic susceptibility as etiological factors related to various cancers. Humans are continuously exposed to many synthetic food additives, one of which is sodium nitrite (NaNO2). There is a direct correlation between increases in consumption of nitrite-treated products and incidence of tissue damage, hepatotoxicity, nephrotoxicity and some types of cancer. The objective of this study was to investigate the protective effects of Trigonella foenum-graecum (TFG) on NaNO2-induced hepatotoxicity and nephrotoxicity. Methods: Forty rats were randomly assigned (10 per group) to control (physiological saline solution), TFG (150 mg/kg/day), NaNO2 (80 mg/kg/day), and NaNO2+TFG (80 mg/kg/day + 150 mg/kg/day) groups. This group was offered TFG seed extract two hours before NaNO2. At the end of three months, the rats were decapitated, and blood, kidney and liver tissues were removed. Results: Three months of oral administration of NaNO2 increased serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, creatinine, and pro-inflammatory cytokine levels in the liver and kidney tissues [except for liver Interleukin 1 alpha (IL-1a)] of rats. Serum AST, ALT, urea, creatinine, liver IL-6, and kidney tumor necrosis factor-a (TNF-a), IL-6, IL-1a levels significantly decreased in the NaNO2+TFG group compared to the NaNO2 group. Pathological examinations, it was determined show that exogenously administered TFG could alleviate the effects of NaNO2 hepatotoxicity and nephrotoxicity. Conclusions: Our results suggest that exogenous TFG mitigates NaNO2-administration induced hepatotoxicity and nephrotoxicity. TFG extract exerted antioxidative and anti-inflammatory effects, and played a significant role in preventing hepatic and renal damage induced by chronic NaNO2 administration.  

scholarly journals A New Method for the Isolation and Purification of Trigonelline as Hydrochloride from Trigonella foenum-graecum L.

2019 ◽  
Vol 16 (3) ◽  
pp. 0550
Author(s):  
Iqbal Hasan AL-Khateeb
Keyword(s):  
Convenient Method ◽  
Quaternary Ammonium ◽  
Classical Method ◽  
Steroidal Saponins ◽  
Physiochemical Properties ◽  
Fenugreek Seeds ◽  
Isolation And Purification ◽  
Trigonella Foenum Graecum ◽  
Other Substances ◽  
Trigonella Foenum Graecum L

Separation of Trigonelline, the major alkaloid in fenugreek seeds, is difficult because the extract of these seeds usually contains Trigonelline, choline, mucilage, and steroidal saponins, in addition to some other substances. This study amis to isolate the quaternary ammonium alkaloid (Trigonelline) and choline from fenugreek seeds (Trigonella-foenum graecum L.) which have similar physiochemical properties by modifying of the classical method. Seeds were defatted and then extracted with methanol. The presence of alkaloids was detected by using Mayer's and Dragendorff's reagents. In this work, trigonilline was isolated with traces of choline by subsequent processes of purification using analytical and preparative TLC techniques. Further identification was done by using HPLC, IR and MP. Pure Trigonelline was isolated from the seeds of Trigonella-foenum graecum excluding other alkaloid like choline. In this study, a new, fast and convenient method for isolation and purification of Trigonelline from fenugreek seeds has been established. Unlike other methods, this one excludes all the non-alkaloidal components from the fenugreek seeds extract.

scholarly journals The effectiveness of Pectin in the reduction of histological changes caused by exposure to monosodium glutamate in female mice: مدى فعالية البكتين في الحد من التغيرات النسيجية التي يسببها تناول جلوتامات أحادي الصوديوم على إناث الفئران

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Sarah Ibrahim Al Othman, Faten khalif Alanazi, Ghada Jaber S
Keyword(s):  
Drinking Water ◽  
Body Weight ◽  
Monosodium Glutamate ◽  
Inflammatory Cells ◽  
Treated Group ◽  
Control Group ◽  
Central Vein ◽  
Histological Changes ◽  
Female Mice ◽  
Liver And Kidney

Monosodium glutamate (MSG) is widely used as a food additive. Excessive consumption of monosodium glutamate has also been shown to affect the liver and kidneys, causing damage to these tissues because of oxidative stress leading to increased production of reactive oxygen species (ROS). The purpose of the study described in this paper was to find out how the liver and kidney toxicity caused by monosodium glutamate can be mitigated using pectin. To this end, 30 albino mice females were divided into four groups. The animals were distributed in special cages. 12-15 weeks with an average body weight of 60 grams. The animals were divided into four groups: the experimental control group (1) comprising 5 female mice were given normal drinking water and the treated group (2) comprising 10 female mice were given monosodium glutamate at a dose of 3 g/kg body weight in drinking water. For three weeks, the treatment group (3) comprising 10 female mice was given pectin at a dose of 300 mg/70 kg body weight in drinking water immediately after the monosodium glutamate dose for three weeks and the pectin group (4) comprising 5 female mice were given Pectin at a dose of 300 mg/70 kg body weight in drinking water for three weeks. The mice were then anesthetized, dissected, and liver and kidney samples were taken from female mice and kept in a 10% neutral formalin solution to make tissue segments. The results showed many histological changes in the liver, such as congestion of the central vein, widening of the sinuses, and the appearance of signs of the death of most hepatocytes, infiltration of the central vein and an invasion of inflammatory cells around the central vein with the emergence of several gaps within the cells. Many of them cavity with the death of most of the tubule cells, the closure of some of them and the expansion and infiltration in others and bleeding inside the tissue. Pectin therapy has led to the disappearance of most of these changes and the emergence of a clear improvement in hepatic and renal tissue.

scholarly journals Beneficial effects of Fenugreek seeds (Trigonella foenum graecum L) added in the diet of diabetic rats

2012 ◽  
Vol 8 (2) ◽  
pp. 1555-1565 ◽  
Author(s):  
Najla Hfaiedh ◽  
Sabah Dhibi ◽  
Sakria Mbarki ◽  
Jean-Claude Murat ◽  
Abdel Fattah Elfeki
Keyword(s):  
Oxidative Stress ◽  
Alkaline Phosphatase ◽  
Thyroid Dysfunction ◽  
Diabetic Rats ◽  
Protective Effects ◽  
Fenugreek Seeds ◽  
Beneficial Effects ◽  
Trigonella Foenum Graecum ◽  
Trigonella Foenum Graecum L ◽  
And Oxidative Stress

Protective effects of Fenugreek seeds (Trigonella foenum graecum L), added in the diet, upon oxidative stress and dysfunctions in kidney, thyroid and liver of alloxan-diabetic rats were investigated.In our study, the alloxan-induced diabetes triggered 1) increased levels of glucose, total cholesterol and triglycerides in blood, 2) increased activities of alkaline phosphatase and transaminases in blood, 3) increased levels of creatinine, urea and protein in blood, 4) a decreased level of TSH and an increased level of free thyroxin in plasma.In addition, an oxidative stress, evidenced by an increase of lipids peroxidation level and superoxide dismutase activity associated with a decrease of glutathione peroxidase and catalase activities in hepatic and renal tissues, was observed.When Fenugreek seeds powder (100g/kg) was added in the food for 30 days, all this parameters were significantly shifted to more normal values.In conclusion, fenugreek seeds powder displays beneficial effects upon hepatotoxicity, nephropathy, thyroid dysfunction and oxidative stress in alloxan-diabetic rats. This property could be attributed to the presence of antioxidant components, such as complex polysaccharides and phenolic acids, as confirmed by analyses. 

scholarly journals Hypoglycemic and hypolipidemic activity of combined milk thistle and fenugreek seeds in alloxan-induced diabetic albino rats

2020 ◽  
Vol 13 (8) ◽  
pp. 1732-1736
Author(s):  
Mohamed Jamal Saadh
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Oral Administration ◽  
Glycosylated Hemoglobin ◽  
Diabetic Rats ◽  
Antidiabetic Drugs ◽  
Albino Rats ◽  
Milk Thistle ◽  
Fenugreek Seeds ◽  
Ameliorative Effect

Background and Aim: Despite the availability of antidiabetic drugs, they are not free from associated adverse side effects. This study aimed to evaluate the hypoglycemic and hypolipidemic effects of oral administration of seeds from two medicinal plants: (1) Milk thistle and (2) fenugreek. Materials and Methods: Plant seeds were washed in distilled water and ground with a coffee grinder. Alloxan was used to induce diabetes in 20 male albino rats. Diabetic rats were randomly divided into two groups: (1) Group 1 (n=10), diabetic rats fed with 0.5 g/kg milk thistle and 2 g/kg fenugreek seeds per day and (2) Group 2 (n=10), diabetic rats fed standard rodent food for 4 weeks. Results: Oral administration of milk thistle and fenugreek seeds for 2 weeks resulted in significant improvement in body weight, blood glucose, glycosylated hemoglobin (HbA1c), cholesterol, and triglyceride levels in alloxan-induced diabetic rats. After 4 weeks, this ameliorative effect was significantly elevated with respect to blood glucose (155.00±9.70 mg/ dL vs. 427.50±5.70 mg/dL; p<0.001), HbA1c (5.5±0.19% vs. 13.65±1.77%; p<0.001), cholesterol (281.50±10.95 mg/dL vs. 334.30±6.80 mg/dL; p<0.001), triglyceride (239.60±6.87 mg/dL vs. 284.20±9.95 mg/dL; p<0.01), and body weight (265.30±8.10 g vs. 207.40±11.4 g; p<0.01) as compared with non-treated diabetic rats. Conclusion: Milk thistle and fenugreek seeds possess hypoglycemic and hypolipidemic properties and could be used as natural compounds that are suitable as parent compounds for the development of new antidiabetic drugs.

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