scholarly journals Detection of epistasis through triple test cross (TTC) analysis in maize (Zea mays L.)

2017 ◽  
Vol 9 (4) ◽  
pp. 2496-2501
Author(s):  
R. Pavan ◽  
E. Gangappa ◽  
S. Ramesh ◽  
A. Mohan Rao ◽  
Hittalmani Shailaja

The present study was carried out to detect the epistasis present in two cross of maize through triple test cross (TTC) analysis. The mean squares due to total epistasis was highly significant at P≤0.01 for all the characters in both C-I and C-II, except for ear length in C-I. The i type of epistasis was highly significant for the traits such as days totasseling, days to silking, earlength, ear circumference, kernels row-1,100 grain weight and shelling percentage in C-I and in C-II, ‘i’ type was non-significant for ASI, ear length, kernels row-1and grain yield plot-1. Both j type and l type of epistasis were significant for all characters in both C-I and C-II, except for ear length in C-I and days to silking in C-II. The estimate of additive genetic component (D) was highly significant for all characters in both C-I and C-II. Epistasis played a significant role in the inheritance of all the characters in both C-I and C-II except for ear length in C-I. Both additive and dominance components of genetic variance with a predominance of dominance genetic variance played an important role in the inheritance of all the quantitative traits except ear length in C-I and kernel rows ear-1 in C-II.

2021 ◽  
Vol 20 (1) ◽  
pp. 17-28
Author(s):  
M.S. Afolabi ◽  
G.O. Agbowuro ◽  
A.E Salami ◽  
M.O. Akoroda

Low-N maize variety is developed for its ability to tolerate low nitrogen soil environment. This experiment was conducted to study the genetic implication of Low-N maize variety for grain yield and related traits under low nitrogen soil conditions triple test cross analysis. Inbred lines used for the study were generated from twelve low nitrogen tolerance open pollinated maize varieties after six generations of selfing. Two inbred lines along with their F1 were used as testers for ten inbred lines in a triple test cross pattern to generate 30 crosses. The 30 crosses, their parents and the testers to State University, Ado-Ekiti during 2017 planting season. The experimental design was a Randomized Complete Block Design (RCBD). Data were collected on plant height, ear height, days to 50% anthesis, days to 50% silking, incidence of curvularia leaf spot, blight, plant aspect, ear aspect, ear rot, stay green, cob per plant, ear weight, grain moisture content and grain yield. All data were subjected to analysis of variance and complete genetic estimates. Additive and dominants were significant (P < 0.05) for all traits; however, epitasis estimates were not significant for all the traits tested. The degree of dominance component indicated partial dominance for all the traits. Correlation coefficients for days to 50% anthesis and 50% silking, plant height, ear height, number of cobs per plant and grain yield were positive and significant (P < 0.05). Since both additive and dominance gene actions were important for low-N traits, the use of reciprocal recurrent selection procedure can be adopted in incorporating the trait into elite maize varieties.


Author(s):  
Lucas Carneiro Maciel ◽  
Weder Ferreira dos Santos ◽  
Joênes Mucci Peluzio ◽  
Osvaldo José Ferreira Junior ◽  
Adriano Silveira Barbosa ◽  
...  

Aims: The research was carried out to evaluate the agronomic performance of corn cultivars in contrasting phosphorus environments in the state of Pará, Brazil. Study Design: The experimental design was randomized blocks with ten treatments and three replications. The treatments were composed of ten cultivars: AG 1051, AG 8088PRO2, BR 206, BRS 3046, PR 27D28, 2B655PW, AL BANDEIRANTE, ANHEMBI, CATIVERDE 02 and ORION Place and Duration of Study: In the 2017/18 crop, two competition trials of corn cultivars were carried out at Sítio Vitória, municipality of Santa Maria das Barreiras, state Pará, Brazil. Methodology: Two competition trials of corn (Zea mays L.) cultivars were carried out under contracting conditions of phosphorus: high P (100 kg ha-1 of P2O5) and low P (50 kg ha-1 of P2O5), using simple superphosphate (18% P2O5) as the source. The agronomic efficiency of phosphorus use was obtained by the difference between grain yield in both levels, divided by the difference between doses. The variables evaluated were: ear height, plant height, ear diameter, number of rows per year, number of grains per rows and grain yield. Results: The mean grain yield of the cultivars ranged from 5,446 kg ha-1 (low P) to 11,486 kg ha-1 (high P), and the means of all cultivars were higher in high P. The means agronomic efficiency ranged from 25.1 kg kg-1 (AL BANDEIRANTE) to 96.6 kg kg-1 (AG 1051). Conclusion: The highest phosphorus dose (100 kg ha-1 of P2O5) resulted in higher values in the variables studied. The cultivars that stood out the most, under high and low phosphorus, were AG 8088PRO2, AG 1051 and BRS 3046, the last two being agronomically efficient.


2002 ◽  
Vol 139 (1) ◽  
pp. 61-66 ◽  
Author(s):  
B. R. BAKHEIT ◽  
M. Z. EL-HIFNY ◽  
M. M. EISSA ◽  
S. B. RAGHEB

The efficiency of the triple test cross (TTC) and the six-population biometrical analyses was compared in terms of assessing and quantifying the components of genetic variance for two faba bean crosses: Triple White×Giza 843 and NA112×Giza 429. Several traits were studied including days to first flower, plant height, branches/plant, pods/plant, seeds/pod, 100-seed weight and seed yield/plant. The results supported the triple test cross biometrical approach as it uses first degree statistics and can be applied to any population irrespective of its genetic architecture. Absence of a scalar relationship between triple test cross families (orthogonality) ensures independence between means and variance with no restrictive assumptions. Both methods provided evidence for epistasis, and both additive and dominance genetic components in the genetic control of the studied traits.


1985 ◽  
Vol 105 (1) ◽  
pp. 123-128
Author(s):  
I. Singh ◽  
R. S. Paroda ◽  
S. Singh

SUMMARYForty-five triple test cross progenies produced by crossing 15 homozygous strains of wheat with three testers (varieties HD 2160 and NP 846 and their F1) and 17 parents, were raised in a randomized-block design in two environments (normal and stress). The data on five randomly chosen plants from parents and F8 and ten plants from F1 x lines were recorded for number of days from sowing to heading, plant height, the number of tillers per plant, total biomass, the number of grains per ear, 1000·grain weight and grain yield per plant. The additive and dominance components of genetic variation were each estimated by three methods to determine their relative usefulness in estimating these components. The variance of parents gave better estimates of the additive component than the two other methods (1i + 2i and 1i + 2i + 3i). The method 1i + 2i + 3i proved its superiority over the 1i + 2i method. The dominance component was better estimated by the method 1i - 2i than by the 4σ2d + 9σ2 (1i + 2i - i) and 25 (σ2 (21i - i) pooled with σ2 (22i - i)) methods. The two methods 4σ2d + 9σ2 (1i + 2i - i) and 25 (2σ2 (21i - i) pooled with σ2 (22i - i)) provided similar results for the estimation of dominance component.


2021 ◽  
Vol 50 (2) ◽  
pp. 351-358
Author(s):  
MT Hasan ◽  
AC Deb

Triple test cross analysis was carried out to detect the epistasis of thirteen yield and yield components in five chickpea (Cicer arietinum L.) crosses. Total epistatic effect was found to be non-significant for all the studied traits. Partitioning of total epistasis indicated the involvement of ‘i’ type (additive × additive) epistasis for DFF, PHFF, PWH, NPd/P, PdW/P, NS/P and SW/P in cross-1; NPBFF and NSBFF in cross-3 and for PHFF, DMF, PHMF and NSBMF in cross-5. The magnitude of additive component (D) was higher than that of the dominance component (H). Partial degree of dominance (√H/D) was observed for most of the traits. Both broad (h2b) and narrow (h2n) sense heritability were found to be moderately high. Positive and significant correlation between sums and differences indicated the direction of dominance towards decreasing parents and vice-versa. Bangladesh J. Bot. 50(2): 351-358, 2021 (June)


2018 ◽  
Vol 6 (1) ◽  
pp. 15-22
Author(s):  
Afolabi M. Segun ◽  
Salami A. Ekundayo

Low-N maize is bred for its ability to tolerate low soil nitrogen (N) by growing and producing grain that compares appreciably to conventional varieties. This experiment was conducted to study the genetic effects of grain yield and other agronomic traits in Low-N maize using triple test cross analysis. Twelve low-N open pollinated maize varieties were converted to the inbred line after six generations of selfing and used for the experiment. Two inbred lines along with their F1 were used as testers for ten inbred lines in a triple test cross pattern to generate 30 crosses and along with their parents and testers to make a total of 43 entries which were evaluated at the Teaching and Research Farms of Ekiti State University, Ado-Ekiti during in 2017. The design was a Randomized Complete Block Design (RCBD). Data was collected on plant height, ear height, days to 50% anthesis, days to 50% silking, the incidence of Curvularia leaf spot, blight, plant aspect, ear aspect, ear rot, stay green, cob per plant, ear weight, grain moisture content and grain yield. All data was subjected to analysis of variance and complete genetic estimates made. Additive and dominants were significant (P0.05) for all traits, however, epitasis estimates were not significant for all the traits. The degree of dominance component indicated partial dominance for all the traits. Correlation coefficients for days to 50% anthesis and 50% silking, plant height, ear height, number of cob per plant and grain yield were positive and significant (P0.05). Since both additive and dominance gene actions were important for low-N traits, the use of reciprocal recurrent selection procedure can be adopted in incorporating the trait into elite maize varieties.


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