DIN EN ISO 6888-1:2022-02, Mikrobiologie der Lebensmittelkette_- Horizontales Verfahren für die Zählung von koagulase-positiven Staphylokokken (Staphylococcus aureus und andere Spezies)_- Teil_1: Verfahren mit Baird-Parker-Agar-Medium (ISO_6888-1:2021); Deutsche Fassung EN_ISO_6888-1:2021

2022 ◽  
Infection ◽  
2006 ◽  
Vol 34 (2) ◽  
pp. 95-97 ◽  
Author(s):  
B. M. W. Diederen ◽  
C. M. van Leest ◽  
I. van Duijn ◽  
P. Willemse ◽  
P. H. J. van Keulen ◽  
...  

2010 ◽  
Vol 48 (4) ◽  
pp. 1510-1510 ◽  
Author(s):  
J. F. Peterson ◽  
K. M. Riebe ◽  
G. S. Hall ◽  
D. Wilson ◽  
S. Whittier ◽  
...  

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Ruth Melliawati ◽  
Sunifah Sunifah

Various studies indicated that endophytic microbes lived in the plant tissues and produced antimicrobial compounds. Sugar-apple plant  (Annona squamosa L) contained alkaloids, cyanogenic glycosides, and flavonoids. The purpose of this reasearch were (1) to determine the endophytic microbes isolated from sugar-apple plant (2) to study inhibiting capabillity of endophytic isolate against Staphylococcus aureus and Candida albicans, (3) to analyze antimicrobial compounds produced by the potential endophytic isolate. Diffusion agar plate methode was used to assessed antimicrobial activity. Antimicrobial compounds were analyzed by Thin Layer Chormatography (TLC) and High Performance Liquid Chormatography (HPLC), compared with erythromycin, metronidazole and tetracycline. Twelve bacterial isolates and 24 fungus were isolated. Selected bacteria, BMC 1.1, showed the biggest clear zone on C. albicans culture on agar medium, meanwhile selected fungi, BTCK 1.1T, formed the biggest colony on S. aureus culture on agar medium. TLC and HPLC analysis showed that the Rf value of BMC 1.1 and BTCK 1.1T chloroform phase fractions was similiar to metronidazole. Metronidazole concentration in C1, C2, Ck1 and Ck2 fraction were 170.98 ppm, 18.27 ppm, 1.51 ppm and 4.14 ppm respectively.


1985 ◽  
Vol 48 (1) ◽  
pp. 21-27 ◽  
Author(s):  
A. CHOPIN ◽  
S. MALCOLM ◽  
G. JARVIS ◽  
H. ASPERGER ◽  
H. J. BECKERS ◽  
...  

Four media were examined for their usefulness in enumerating Staphylococcus aureus inoculated (a) into milk that was then dried or (b) directly into dried milk powder. In all, seven strains of S. aureus were inoculated individually into each preparation and were enumerated after two periods of storage (18 to 19 d and 60 to 61 d). Fourteen laboratories from twelve countries participated in the comparison which found that direct plating on agar medium in 14-cm petri dishes may be as useful as enrichment followed by streaking. Plating on Baird-Parker medium or on Hauschild pork plasma fibrinogen medium and a MPN method using Giolitti and Cantoni's broth with Tween 80 were equally sensitive for enumerating S. aureus in dried milk powder. The use of Hauschild medium may eliminate the need for supplementary tests to confirm colonies as S. aureus, but in some cases was found to fail in some laboratories. Giolitti and Cantoni's broth without Tween 80 generally was less useful than the three other media for enumerating S. aureus. S. aureus inoculated into milk that was then dried survived longer than when inoculated into dried milk.


1976 ◽  
Vol 22 (11) ◽  
pp. 1603-1611 ◽  
Author(s):  
J-J. Devoyod ◽  
Liliane Millet ◽  
G. Mocquot

A selective agar medium (pork plasma medium for S. aureus (PPSA)) enables the direct enumeration of coagulase-positive staphylococci. This medium is based on the Baird-Parker agar without egg yolk and is supplemented with pig plasma. Colonies of Staphylococcus aureus are surrounded by a halo of precipitated fibrin. When foods such as dairy products contain large numbers of egg yolk – negative strains of S. aureus, the PPSA agar has the advantage over egg yolk containing media such as Baird-Parker agar that fewer suspect colonies have to be confirmed.


2003 ◽  
Vol 48 ◽  
pp. 39-46
Author(s):  
Dragi Todorovic ◽  
Ana Sokolovska ◽  
Hristina Babunovska

A validation of the method for determination of the microbiological purity of Caffetin® tablets has been done. For this purpose the test microorganisms: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and Aspergillus niger from the collection ATCC have been used. A non-specific nutritious medium for aerobic microorganisms and specific nutritious media for adequate test microorganisms: ENDO, Cetrimid, Baird-Parker and Sabouraud nutritious agar medium have been used. The method was performed in two modes: a direct inoculation into a nutritious medium and a membrane filtration. At the same time, a Challenge test was as well used the test of counting the growing colonies (CFU/ml). A calculation of the factor has been done, which represents relationship between growing microorganisms of the inoculated nutritious medium with and without adding to the examined preparation, as a criterion for acceptance of the achieved analytical results. The achieved values for the factor as a criterion for acceptance have shown satisfying values. It can be concluded that this method can be used for determination of the microbiological purity of Caffetin® tablets.


1977 ◽  
Vol 5 (6) ◽  
pp. 604-609
Author(s):  
H A Linke

A new, improved agar medium for the isolation of Streptococcus mutans, the etiological agent of dental caries, was developed. In contrast to mitis-salivarius agar, this medium not only recovers a greater number of S. mutans strains from most oral specimens but, because of its mannitol and sorbitol content, it also facilitates the differentiation of S. mutans from other oral streptococci, e.g., S. salivarius, S. mitis, and S. sanguis, which do not grow or produce scanty growth only after 10 days of incubation. The medium is easy to prepare because of its simple and unique composition, is characterized by the presence of an acid indicator, and can be utilized under aerobic and anaerobic conditions as well. The medium cannot be used to distinguish among the eight serotypes, a to g and SL-1, of S. mutans. Mannitol-utilizing bacteria such as streptococci (e.g., S. faecalis) and other microorganisms (e.g., Staphylococcus aureus) are able to grow on this medium and can be distinguished from S. mutans by their unique colony morphology.


2018 ◽  
Vol 10 (03) ◽  
pp. 265-270 ◽  
Author(s):  
Seyedali Seyedmajidi ◽  
Ramazan Rajabnia ◽  
Maryam Seyedmajidi

ABSTRACT AIMS AND OBJECTIVES: Infection is a serious problem for patients after implantation surgery, which is difficult to treat with antibiotic therapy. The present study was developed to evaluate and compare the antibacterial properties of hydroxyapatite/bioactive glass (HA/BG) and fluorapatite/bioactive glass (FA/ BG) nanocomposite foams as a cellular scaffold for use in bone defects by two macrodilution and disk diffusion methods. MATERIALS AND METHODS: Staphylococcus aureus, Enterococcus faecalis, and Streptococcus mutans were cultured in brain heart infusion broth medium with nanocomposite powder for 5 days, and their bioactivity levels were evaluated by daily culturing on solid agar medium plates. To carry out the disk diffusion test, a disc form of nanocomposite foams was used on agar medium with 48 h incubation. Results: None of two nanocomposites even at their highest concentration (200 mg/mL) did not prevent the growth of two Staphylococcus aureus and Enterococcus faecalis microorganisms. However, HA/BG nanocomposite on the 3rd day at a concentration of 200 mg/mL and on 4th and 5th day at a concentration of 100 mg/mL and FA/BG nanocomposite on the 4th day at a concentration of 100 mg/mL and on the 5th day at a concentration of 50 mg/mL could be able to kill Streptococcus mutans microorganism. In the disc diffusion test, none of the nanocomposites could create a nongrowth zone. Both tested biomaterials showed increased antibacterial properties over time and concentration increase. Conclusions: HA/BG and FA/BG nanocomposites, due to their biocompatibility and antimicrobial properties, are good choices for implantation instead of damaged bone tissue in tissue engineering.


2003 ◽  
Vol 41 (12) ◽  
pp. 5695-5698 ◽  
Author(s):  
J. D. Perry ◽  
C. Rennison ◽  
L. A. Butterworth ◽  
A. L. J. Hopley ◽  
F. K. Gould

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