scholarly journals Effect of N-Methyl-D-Aspartate Receptor Blockade on Anxiety-Like Behavior Induced in Rats by Postnatal Chronic Exposure to the Endocrine Disruptor Di-2 (Ethyl-Hexyl Phthalate) in Elevated plus Maze Test

2019 ◽  
Vol 3 (4) ◽  
Keyword(s):  
Chronic Exposure ◽  
Endocrine Disruptor ◽  
Elevated Plus Maze ◽  
Receptor Blockade ◽  
Elevated Plus Maze Test ◽  
Aspartate Receptor ◽  
Maze Test ◽  
Ethyl Hexyl ◽  
Plus Maze

Anticonvulsant, Anxiolytic and Antidepressant Properties of the β-caryophyllene in Swiss Mice: Involvement of Benzodiazepine-GABAAergic, Serotonergic and Nitrergic Systems

2020 ◽  
Vol 14 (1) ◽  
pp. 36-51 ◽  
Author(s):  
George L. da Silva Oliveira ◽  
José C. Correia L. da Silva ◽  
Ana P. dos Santos C. L da Silva ◽  
Chistiane M. Feitosa ◽  
Fernanda R. de Castro Almeida
Keyword(s):  
Receptor Antagonist ◽  
Molecular Mechanisms ◽  
Elevated Plus Maze ◽  
Feeding Test ◽  
Swiss Mice ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze ◽  
Gabaergic Receptors ◽  
Pre Treatment

Background: Central nervous system disorders such as anxiety, depression and epilepsy are characterized by sharing several molecular mechanisms in common and the involvement of the L-arginine/NO pathway in neurobehavioral studies with β-caryophyllene is still little discussed. Objectives: One of the objectives of the present study was to demonstrate the anxiolytic behavioral effect of β-caryophyllene (β-CBP) in female Swiss mice, as well as to investigate the molecular mechanisms underlying the results obtained. Methods: This study evaluated the neurobehavioral effects of β-CBP using the open field test, rota-rod test, elevated plus maze test, novelty suppressed feeding test, tail suspension test and forced swim test, as well as pilocarpine, pentylenetetrazole and isoniazid-induced epileptic seizure models. Results:: The results demonstrated that the neuropharmacological activities of β-CBP may involve benzodiazepine/GABAergic receptors, since the pre-treatment of β-CBP (200 mg/kg) associated with flumazenil (5 mg/kg, benzodiazepine receptor antagonist) and bicuculline (1 mg/kg, selective GABAA receptor antagonist) reestablished the anxiety parameters in the elevated plus-maze test, as well as the results of reduced latency to consume food in the novelty suppressed feeding test. In addition to benzodiazepine/GABAergic receptors, the neuropharmacological properties of β-CBP may be related to inhibition of nitric oxide synthesis, since pre-treatment with L-arginine (500- 750 mg/kg) reversed significantly the anxiolytic, antidepressant and anticonvulsant activities of β-CBP. Conclusion: The results obtained provide additional support in understanding the neuromolecular mechanisms underlying the anxiolytic, antidepressant and anticonvulsive properties of β-CBP in female Swiss mice.

scholarly journals Effects of test experience, closed-arm wall color, and illumination level on behavior and plasma corticosterone response in an elevated plus maze in male C57BL/6J mice: a challenge against conventional interpretation of the test

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Hirotaka Shoji ◽  
Tsuyoshi Miyakawa
Keyword(s):  
Elevated Plus Maze ◽  
Plasma Corticosterone ◽  
Illumination Level ◽  
Test Battery ◽  
Lower Percentage ◽  
Behavioral Tests ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze ◽  
Corticosterone Response

AbstractThe elevated plus maze test is a widely used test for assessing anxiety-like behavior and screening novel therapeutic agents in rodents. Previous studies have shown that a variety of internal factors and procedural variables can influence elevated plus maze behavior. Although some studies have suggested a link between behavior and plasma corticosterone levels, the relationships between them remain unclear. In this study, we investigated the effects of experience with a battery of behavioral tests, the wall color of the closed arms, and illumination level on the behavior and plasma corticosterone responses in the elevated plus maze in male C57BL/6J mice. Mice were either subjected to a series of behavioral tests, including assessments of general health and neurological function, a light/dark transition test, and an open field test, or left undisturbed until the start of the elevated plus maze test. The mice with and without test battery experience were allowed to freely explore the elevated plus maze. The other two independent groups of naïve mice were tested in mazes with closed arms with different wall colors (clear, transparent blue, white, and black) or different illumination levels (5, 100, and 800 lx). Immediately after the test, blood was collected to measure plasma corticosterone concentrations. Mice with test battery experience showed a lower percentage of open arm time and entries and, somewhat paradoxically, had lower plasma corticosterone levels than the mice with no test battery experience. Mice tested in the maze with closed arms with clear walls exhibited higher open arm exploration than mice tested in the maze with closed arms with black walls, while there were no significant differences in plasma corticosterone levels between the different wall color conditions. Illumination levels had no significant effects on any measure. Our results indicate that experience with other behavioral tests and different physical features of the maze affect elevated plus maze behaviors. Increased open arm time and entries are conventionally interpreted as decreased anxiety-like behavior, while other possible interpretations are considered: open arm exploration may reflect heightened anxiety and panic-like reaction to a novel situation under certain conditions. With the possibility of different interpretations, the present findings highlight the need to carefully consider the test conditions in designing experiments and drawing conclusions from the behavioral outcomes in the elevated plus maze test in C57BL/6J mice.

Anxiety levels and wild running susceptibility in rats: assessment with elevated plus maze test and predator odor exposure

2005 ◽  
Vol 68 (2) ◽  
pp. 135-144 ◽  
Author(s):  
Hugo Medeiros Garrido de Paula ◽  
Amauri Gouveia ◽  
Marcos Vinícius de Almeida ◽  
Katsumasa Hoshino
Keyword(s):  
Elevated Plus Maze ◽  
Predator Odor ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze ◽  
Anxiety Levels ◽  
Odor Exposure

Assessment of amphetamine withdrawal anxiety-like behavior in the elevated plus-maze test in rats

2011 ◽  
Vol 63 (2) ◽  
pp. 560-561
Author(s):  
Dorota Kołtunowska ◽  
Ewa Gibuła-Bruzda ◽  
Jolanta H. Kotlińska
Keyword(s):  
Elevated Plus Maze ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze ◽  
Amphetamine Withdrawal

scholarly journals Synergistic Action of Sodium Selenite with some Antidepressants and Diazepam in Mice

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 270
Author(s):  
Ewa Kędzierska ◽  
Lila Dąbkowska ◽  
Paweł Obierzyński ◽  
Magdalena Polakowska ◽  
Ewa Poleszak ◽  
...  
Keyword(s):  
Locomotor Activity ◽  
Sodium Selenite ◽  
Elevated Plus Maze ◽  
Synergistic Action ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze ◽  
Immobility Time ◽  
Combined Administration ◽  
Antidepressant Action

Background: The antidepressant and anxiolytic effects of selenium (Se) have been proven in many studies. This work was aimed at confirming these activities of its inorganic form—sodium selenite—and examining the possible synergy of action with antidepressants and diazepam. Methods: The antidepressant- and anxiolytic-like activity of Se was assessed using forced swim tests (FSTs) and elevated plus-maze test (EPMs). Spontaneous locomotor activity was measured using photoresistor actimeters. The experiments were conducted on male Albino Swiss mice. Results: Sodium selenite (0.5 mg/kg) reduced the immobility time in the FSTs and extended time spent in the open arms of EPMs without affecting locomotor activity The combined administration of Se at an ineffective dose (0.25 mg/kg) together with imipramine (15 mg/kg), fluoxetine (5 mg/kg), tianeptine (10 mg/kg), but not with reboxetine (2.5 mg/kg), resulted in a reduction of immobility time in FSTs, and with a threshold dose of diazepam (0.25 mg/kg) led to the prolongation of time spent in the open arms of the EPM. Moreover, the antidepressant-like effect of Se (0.5 mg/kg) was significantly reduced by pretreatment with p-chlorophenylalanine (100 mg/kg). Conclusions: The results may indicate the participation of serotonergic transmission to antidepressant action of Se and GABA-ergic transmission to its anxiolytic effects.

scholarly journals The novel dipeptide ligand of TASP

2019 ◽  
Vol 484 (2) ◽  
pp. 228-232
Author(s):  
O. A. Deeva ◽  
A. S. Pantileev ◽  
I. V. Rybina ◽  
M. A. Yarkova ◽  
T. A. Gudasheva ◽  
...  
Keyword(s):  
Elevated Plus Maze ◽  
Open Field Test ◽  
Anxiolytic Activity ◽  
The Novel ◽  
Minimum Effective Dose ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze ◽  
Order Of Magnitude ◽  
Preliminary Administration

Using the previously obtained first dipeptide ligand TSPO the N‑carbobenzoxy-L‑tryptophanyl-L‑isoleucine amide (GD‑23) as a basis, the new dipeptide was synthesized — the N‑phenylpropionyl–L‑tryptophanyl-L‑leucine amide (GD‑102). GD‑102 expressed anxiolytic activity in the open field test in BALB/c mice and in the elevated plus maze test in ICR mice. The minimum effective dose of GD‑102 was an order of magnitude lower than that of GD‑23. Preliminary administration of the TSPO selective antagonist, compound PK11195, completely blocked the anxiolytic activity of GD‑102, that indicated the participation of TSPO in the realization of the anxiolytic action GD‑102. The results were confirmed by molecular docking data.

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