Natural bee honey and Conservation of drone semen in liquid nitrogen

Author(s):  
A. Gulov ◽  
A. Laskin

Purpose: Conducting a honey diluent test for creeples of sperm of a drone honey bee.Materials and methods. The material for the research served a sperm of the milled drone drums of the "Prioksky" type of the Midway breed of bees. The selection of sperm was carried out in June-July 2020 g by the method of artificial stimulation of the turning of the endofalosha in half-armed drones aged 25-30 days. The rock type "Prioksky" of the middle Russian breed of bees. Before freezing, the sperm was stored in glass capillaries in the cooled state at 3 ° C for 2 months. The following composition of the diluent was tested - 10% honey, lactose, sucrose, egg yolk and dimethyl sulfoxide.Results. Studies have shown the viability of sperm at 64.0 ± 1.8% (41.5-83.7), and a total mobility of 2.2 ± 0.6% (0-11.5). To evaluate the fertilizing ability of sperm, carried out artificial insemination of 10 bee modules. In 4 seeded bees dykens, the presence of sperm in a seed-hearter with a concentration of sperm from 0.22-4.4 million / μl is revealed. In paired eggs of three other seeded matters, the presence of sperm and the complete absence of spermatozoa in the seed-receptionist are recorded.Conclusion. Tests of the honey diluent for deep freezing sperm of the drone honey bees in liquid nitrogen confirmed its cryophylactic properties.

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1801
Author(s):  
Elena Nikitkina ◽  
Artem Musidray ◽  
Anna Krutikova ◽  
Polina Anipchenko ◽  
Kirill Plemyashov ◽  
...  

The fertilizing ability of stallion sperm after freezing is lower than in other species. The search for the optimal extender, combination of extenders, and the freezing protocol is relevant. The aim of this study was to compare lactose-chelate-citrate-yolk (LCCY) extender, usually used in Russia, and Steridyl® (Minitube) for freezing sperm of stallions. Steridyl is a concentrated extender medium for freezing ruminant semen. It already contains sterilized egg yolk. Semen was collected from nine stallions, aged from 7 to 12 years old. The total and progressive motility of sperm frozen in Steridyl was significantly higher than in semen frozen in LCCY. The number of spermatozoa with normal morphology in samples frozen in LCCY was 60.4 ± 1.72%, and with Steridyl, 72.4 ± 2.10% (p < 0.01). Semen frozen in Steridyl showed good stimulation of respiration by 2.4-DNP, which indicates that oxidative phosphorylation was retained after freezing–thawing. No differences among the extenders were seen with the DNA integrity of spermatozoa. Six out of ten (60%) mares were pregnant after artificial insemination (AI) by LCCY frozen semen, and 9/12 (75%) by Steridyl frozen semen. No differences among extenders were seen in pregnancy rate. In conclusion, Steridyl was proven to be a good diluent for freezing stallion semen, even though it was developed for ruminants.


2017 ◽  
Author(s):  
Siti Aminah Zakaria ◽  
Zuhailawati Hussain ◽  
Anasyida Abu Seman

Behaviour ◽  
1963 ◽  
Vol 22 (1-2) ◽  
pp. 152-166 ◽  
Author(s):  
Axel Michelsen

AbstractA description is given of the sexual behaviour of 16 longicorn beetles of the subfamily Lepturinae. The following points have been investigated:- 1. Stimulation of the female performed by the male either as a "licking" or combined "licking and tapping" of the female elytra or the grasping of an antenna of the female. 2. Movements of the antennae of the male, which seem to be an expression of the "state of excitement" of the male. These movements are in principle thought to be one of the behaviour patterns originally present in the order Coleoptera. 3. A "round dance", one of the methods for the female to throw off the male after copula, is described. 4. A selection of partner according to size is described in the genus Rhagium. 5. A comparative analysis is attempted for some species and systematic conclusions of such comparisons are proposed.


2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Jamiat . ◽  
Iskandar A M ◽  
M Idham

Local wisdom is one of the characteristics national culture that deserves to be explored and developed in the future. Honey production is carried out by maintaining traditional nest making that utilizes natural materials are local wisdom that is still applied. The purpose of this study is to (1) reveal the local wisdom in natural forest honey management, (2) the amount of natural forest honey produced by the community from periau using tikung techniques in the area of Siawan Belida, Nanga Tuan Village, Bunut Hilir Subdistrict, Kapuas Hulu Regency. The method used a survey with interview techniques, the selection of respondents are choosing all Nanga Tuan periau. The results of the study revealing local wisdom in preserving natural honey bees with tikung techniques in the Siawan Belida area, namely; tikung, making tikung, selecting trees for installation of tikung, agreement on division of territory in lakes (suak), sanctions for tree destroyers, sanctions for tikung hives burglars, pre-harvesting ceremonies, conditions of harvesting natural forest honey, harvesting honey bees process, honey treatment after harvesting at home. Tree preservation efforts of tikung honey bee techniques based on local wisdom, including; stipulation of forest lake areas in the management of tikung honey, types of feed for Apis dorsata bees. The amount of natural forest honey is 5- 8 tons / year with planting done twice / year.Keywords : local wisdom, natural honey bees, Siawan Belida, tikung techniques


2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Hidas Karina Ilona ◽  
Ildikó Csilla Nyulas-Zeke ◽  
László Friedrich ◽  
Anna Visy ◽  
Judit Csonka ◽  
...  

Eggs are widely utilized because of their high nutrient value, coagulating, foaming, emulsifying and sometimes even colouring or flavouring facilities in food manufacturing. Production of processed egg products shows an increasing trend. Frozen products belong to first processing, their shelf life can increase up to 1 year. By freezing, a large reduction in microbial loss can be achieved. But different undesirable processes can occur. The effect of freezing on animal cells is highly dependent on freezing parameters. It has a different effect on egg subtituents. Egg yolk undergoes a gelation process while proteins can denaturate. In our study pasteurized liquid egg products (liquid egg white, liquid egg yolk and liquid whole egg) were frozen by dripping into liquid nitrogen. After that, a 14-day frozen storage experiment was carried out at -18°C. Before freezing and on the 1th, 7th and 14th days of storage experiment pH, dry matter content, colour and calorimetric properties (denaturation temperatures and enthalpy of denaturation) with differential scanning calorimetry were tested. For statistical analysis, one-way ANOVA (α = 0.05) was employed. In our experiment, we found no significant change in calorimetric properties of liquid egg white after freezing, but significant decreasing of enthalpy and denaturation temperatures of liquid egg yolk and liquid whole egg was identified. In contrast, frozen storage had a decreasing effect in all these products. Freezing caused a clearly visible colour change in LEW, a visible change in colour of LWE and a very clearly visible change in colour of LEY. In case of LEW and LEY changes increased to clearly visible 14 days. In conclusion, our results show that frozen storage had a greater effect on liquid egg products properties than freezing in liquid nitrogen.


2012 ◽  
Vol 15 (20) ◽  
pp. 979-985 ◽  
Author(s):  
Nur Ducha ◽  
T. Susilawati ◽  
Aulanni`am . ◽  
Sri Wahyunings ◽  
Mulyoto Pangestu

2015 ◽  
Vol 48 (2) ◽  
pp. 99-108 ◽  
Author(s):  
H.F. Abou-Shaara

AbstractThere are various plants with potential feeding importance to honey bee, Apis mellifera, colonies as source of pollen, nectar or both. Selection of suitable regions for apiaries mainly depends on the availability of honey bee plants in the apiary region. Identifying honey bee plants in specific region is very essential for honey and pollen production from honey bee colonies. Lacking the information about the beneficial plants for honey bees including; plant name, flowering time and potential benefit to honey bee colonies could be considered as a limitation for beekeeping development. So far honey bee plants are not well studied in Egypt. This review paper presents potential honey bee plants in Egypt using the available publications. The studies on honey bee plants in Egypt were also reviewed. This work can be considered as a guide for beekeepers and researchers. Moreover, the presented plants here can be used in comparing honey bee plants of Egypt with other countries to get a better understanding of honey bee flora. More detailed investigations on honey bee plants are strongly required to be done at all Egyptian Governorates


1980 ◽  
Vol 17 (2) ◽  
pp. 226-233 ◽  
Author(s):  
J. Y. S. Lee ◽  
P. B. Little

Lesions of cerebrocortical necrosis experimentally induced in pigs by transcalvarial freezing with liquid nitrogen were slightly fluorescent 48 hours after surgery. Fluorescence increased greatly thereafter and was most marked grossly under ultraviolet illumination with a wavelength of 366 nm at 7 days. Fluorescence of the necrotic focus persisted up to 35 days after surgery. Detection of cerebrocortical necrosis by ultraviolet illumination of fresh specimens during gross inspection is useful in determining the extent and distribution of lesions. This would aid more accurate selection of brain sections for histological examination in various encephalopathies.


2010 ◽  
Vol 636-637 ◽  
pp. 1292-1299 ◽  
Author(s):  
Carlos Alves ◽  
Carlos Figueiredo ◽  
Paula Figueiredo ◽  
António Maurício ◽  
Luís Aires-Barros

Several types of stones (igneous, sedimentary and metamorphic) are found applied in pavements, stairs and walls of the underground stations of the Lisbon Metropolitan System, Portugal. A field study based solely on non-destructive and non invasive visual inspection of these stations is presented, aiming to identify different transformations of applied stones and discuss its relation with the stone characteristics. This field study has pointed to relevant issues regarding durability of stone materials namely when used in pavements and stairs. The most striking aspect is the frequently marked variable behaviour of stones from a given rock type under the same use conditions, with heterogeneities and discontinuities that seem to cause irregularity from the early stages and further increase with time, having more accentuated consequences (in terms of intensity of surface irregularity) in some types of limestones. The observations collected could be relevant for the discussion of characteristics relevant for the quality control of these materials (namely at the quarry level), including the selection of stones for future substitutions. There is, therefore, the need for a more complete study comprising detailed petrographic studies and laboratory tests both from the applied stones and from the supplying quarries.


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