scholarly journals TOPICAL APPLICATION OF TERMINALIA CHEBULA CREAM INHIBITS ULTRAVIOLET-B-INDUCED PSORIASIS IN RATS

2020 ◽  
pp. 15-20
Author(s):  
Aruna Pai ◽  
Jvln Seshagiri Rao ◽  
Madhu J Rajendra ◽  
Ashish S Jain
Keyword(s):  
Topical Application ◽  
Water Extract ◽  
Reducing Power ◽  
Ultraviolet B ◽  
Total Phenolic ◽  
Terminalia Chebula ◽  
Epidermal Thickness ◽  
Reducing Ability ◽  
Dpph Method ◽  
Anti Oxidant

Introduction: The present study aims at evaluating anti oxidant activity and inhibition of topical application of Terminalia chebula cream in ultraviolet –B-induced psoriasis in rats. Materials &Methods: The seeds of Terminalia chebula were dried and powder was extracted with chloroform, ethyl acetate, ethanol, methanol, and deionized water by cold maceration method. Total phenolic content, total tannin content and total triterpenoid content was determined by different methods. Anti oxidant activity was evaluated by DPPH method and reducing power assay. Cream formulation of the optimized extract was prepared by hot fusion method. Psoriasis was induced by irradiatiating for 30 min at a vertical distance of 20 cm with UV light (385 nm) on the skin. Severity index, hydroxyproline content and epidermal thickness was determined. Results and Discussion: Total Phenol (mg gallic acid/ g) content, Total Tannins (mg catechin/ g) content and  Total triterpenoid (mg ursolic acid/ g) content was found to be significant for water extract.The reducing ability of water extract was always higher than methanol extract at all the concentrations. Conclusion: We concluded that cream containing 0.1% water extract of Terminalia chebula seeds possess antioxidant activity along with potent antipsoriatic activity. Further, application of Terminalia chebula cream resulted in decrease in relative epidermal thickness of animal skin as well as other histopathological features. The present investigation aims at the development of potent phytomedicine for treatment of psoriasis from the Terminalia chebula plant.

scholarly journals SettingsTOPICAL APPLICATION OF TERMINALIA CHEBULA CREAM INHIBITS ULTRAVIOLET-B-INDUCED PSORIASIS IN RATS

2020 ◽  
Vol 3 (3) ◽  
pp. 15-20
Author(s):  
Aruna Pai ◽  
Jvln Seshagiri Rao ◽  
Madhu Rajendra ◽  
Ashish Jain
Keyword(s):  
Water Extract ◽  
Reducing Power ◽  
Severity Index ◽  
Ultraviolet B ◽  
Total Phenolic ◽  
Terminalia Chebula ◽  
Epidermal Thickness ◽  
Reducing Ability ◽  
Dpph Method ◽  
Anti Oxidant

Introduction: The present study aims at evaluating anti oxidant activity and inhibition of topical application of Terminalia chebula cream inultraviolet –B-induced psoriasis in rats.Materials and Methods: The seeds of Terminalia chebula were dried and powder was extracted with chloroform, ethyl acetate, ethanol,methanol, and deionized water by cold maceration method. Total phenolic content, total tannin content and total triterpenoid content wasdetermined by different methods. Anti oxidant activity was evaluated by DPPH method and reducing power assay. Cream formulation of theoptimized extract was prepared by hot fusion method. Psoriasis was induced by irradiatiating for 30 min at a vertical distance of 20 cm withUV light (385 nm) on the skin. Severity index, hydroxyproline content and epidermal thickness was determined.Results: Total Phenol (mg gallic acid/ g) content, Total Tannins (mg catechin/ g) content and Total triterpenoid (mg ursolic acid/ g) contentwas found to be significant for water extract.The reducing ability of water extract was always higher than methanol extract at all theconcentrations.Conclusion: We concluded that cream containing 0.1% water extract of Terminalia chebula seeds possess antioxidant activity along withpotent antipsoriatic activity. Further, the application of Terminalia chebula cream resulted in decrease in relative epidermal thickness of animalskin as well as other histopathological features. The present investigation aims at the development of potent phytomedicine for treatment ofpsoriasis from the Terminalia chebula plant.

scholarly journals Phytochemical content and antioxidant activities of Rhubarb (Rheum emodi)

2021 ◽  
Author(s):  
Ahmed Shahwan ◽  
Naema El Aali ◽  
Yusra Layas ◽  
Salem G. El Tumi
Keyword(s):  
Antioxidant Activities ◽  
Water Extract ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Content ◽  
Reducing Ability ◽  
Reducing Power Assay ◽  
Novel Antioxidants ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Medicinal plants have varying natural products and several antioxidants. Antioxidants play a principal function to protect against destruction caused by oxidative stress (OS), <i>Rheum emodi</i> is not an exception, in which it is reported to have compounds that possess antioxidant activity, like polyphenolic compounds. In addition to that, other compounds have proven to have antidiabetic, antimicrobial, antifungal, cytotoxic, hepatoprotective, and nephroprotective activities. This study aimed to quantify the water extract of a powdered plant of <i>R. emodi</i> to evaluate its ability to scavage free radicals. Total phenolic, total flavonoid contents, and reducing ability were measured to consider possible sources of future novel antioxidants in plants. The TPC, TFC, and reducing power assay increased with increasing concentrations of <i>R. emodi</i>. At 1000 µg/ml, the absorbance ranged from 1.437 for TPC, 1.602 for TFC, and 1.638 for reducing power assay, which is more than the absorbance of the standards at the same concentration. The extracted content of reducing power, phenolic, and flavonoid compounds were higher than the standard pyrogallol, quercetin, and ascorbic acid.

scholarly journals Assessment of flavonoids and phenolic compound accumulation in invasive Solidago canadensis L. in Slovakia

10.5219/1378 ◽  
2020 ◽  
Vol 14 ◽  
pp. 587-594
Author(s):  
Olga Shelepova ◽  
Yulia Vinogradova ◽  
Olena Vergun ◽  
Olga Grygorieva ◽  
Ján Brindza
Keyword(s):  
Phenolic Compounds ◽  
Therapeutic Potential ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Raw Material ◽  
Herbal Remedies ◽  
Total Phenolic ◽  
Solidago Canadensis ◽  
Dpph Method

Solidago canadensis L. was introduced to Europe as an ornamental plant from North America in 1645 and began to spread during the XIX-XX centuries. Nowadays the species is considered the most aggressive invasive species. On the other hand, S. canadensis is considered to be a medicinal plant. The raw material known as Herba Solidaginis includes herbs of S. canadensis, S. gigantea, and S. virgaurea. These species are known for their diuretic, anti-inflammatory, antimicrobial, antioxidant, antispasmodic properties. The purpose of our study was to analyze the chemical compounds and some biological properties of S. canadensis, growing in Slovakia, to evaluate its therapeutic potential. The total phenolic content (TPC) of the extracts from aerial parts of S. canadensis was determined by the Folin-Ciocalteu method. The evaluation of total flavonoid content (TFC) was performed by using a spectrophotometric method. The flavonoids content was expressed as rutin equivalents (mg REs) per g DW vegetal product. The phytochemical profile of S. canadensis extracts was assessed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The radical scavenging activity of samples was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Reducing power of extracts was determined by the phosphomolybdenum method. Total phenolic contents (TPC) and total flavonoid contents (TFC) of the extracts varied from 204.19 to 293.43 mg GAE.g-1 DW, and 64.99 – 175.25 g QE.g-1 DW, respectively; the best results were obtained for ethanol extract. Some phenolic compounds were identified by HPLC with significant amounts of rutin (211.20 µg.mL-1), quercetin (122.08 µg.mL-1), quercitrin (102.50 µg.mL-1) and chlorogenic acid (147.00 µg.mL-1). The DPPH values in the inflorescences were higher than in the leaves: the antioxidant activity of leaf extracts was in the range from 5.34 to 17.16 mg TE.g-1, for inflorescences, this parameter ranged from 6.09 to 19.87 mg TE.g-1. The high total phenolic compounds and flavonoids can be used as a valuable source of phytochemicals in herbal remedies. Our study of S. canadensis, growing in Slovakia, shows the promising potential that can be evaluated as an effective antioxidant and antimicrobial agent in herbal medicines.

scholarly journals Antioxidant and cytotoxic activities of Ageratum conyzoides stems

2013 ◽  
Vol 2 (2) ◽  
pp. 33-37 ◽  
Author(s):  
Fatema Nasrin
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Reducing Power ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Ageratum Conyzoides ◽  
Reducing Ability ◽  
Ic50 Value ◽  
Total Antioxidant

Modern civilization is facing more than hundreds of disorders associated with free radicals and natural antioxidants from non-edible plants are gaining importance to fight these disorders. The intention of this report is to evaluate a well known medicinal weed Ageratum conyzoides stems for its antioxidant and cytotoxic Effects. Antioxidant potentiality of the crude methanolic extract of the Ageratum conyzoides (AC) stems was investigated on DPPH scavenging activity, reducing ability, total antioxidant capacity as well as total phenolic contents. Cytotoxic study was done by brine shrimp lethality bioassay and vincristin sulphate was used as standard. The total phenols and total antioxidant capacity of AC was found to be 38.125 ± 2.01mg/g equivalent of gallic acid and 333.37 ± 4.22mg/gm equivalent of ascorbic acid, respectively. The percentage (%) scavenging of DPPH free radical of the extract was found to be concentration dependent with IC50 value 46.01 ± 2.23µg/ml while IC50 value of standard ascorbic acid was found to be 29.56 ± 0.11?g/ml. The reducing power of AC was found to be concentration dependent. The cytotoxicity exhibited by AC was found promising with LC50 value 1.32?g/ml, comparing with the LC50 (0.689?g/ml) values of vincristin sulphate. The present investigation suggests that Ageratum conyzoides possesses remarkable antioxidant and cytotoxic property.DOI: http://dx.doi.org/10.3329/icpj.v2i2.13195 International Current Pharmaceutical Journal 2013, 2(2): 33-37

scholarly journals Comparative study of the antioxidant properties of silymarin and milk thistle extracts

2021 ◽  
Vol 19 (1) ◽  
pp. 1-6
Author(s):  
V. Ivanov ◽  
M. Pavlova
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Water Extract ◽  
Total Phenolic ◽  
Milk Thistle ◽  
Flavonoid Content ◽  
Silybum Marianum ◽  
Dpph Method ◽  
Phenolic And Flavonoid

Purpose: Milk thistle (Silybum marianum (L.) Gaertn) has been used for centuries as a natural remedy for liver and gall bladder / biliary tract diseases. Silymarin is the purified and standardized extract from milk thistle seeds. The purpose of the present study is to present a comparative analysis of the total phenolic substances content and the antioxidant effect of silymarin and three different types of Silybum marianum extracts (i.e. ethanol extract, water extract and lyophilized extract). Methods: Total phenolic and flavonoid content of extracts was analysed and the antioxidant activity was assessed by determining their free radical scavenging ability by DPPH method. Results: Total phenolic content is the highest in silymarin– 134.12±3.22 mg GAE/g, followed by lyophilized water extract – 51.74±2.10 mg GAE/g. Silymarin has also been found to have the highest antioxidant activity among all extract samples (338.03 VCEAC µmol /g), followed by S.marianum lyophilised extract (123.05 VCEAC µmol/g). Conclusions: A strong correlation between total phenolic and flavonoid content in the extracts and their antioxidant activity has been found.

scholarly journals Anti-oxidant activity of methanolic extract of aquatic flowering plant Nymphaea capensis leaf

2020 ◽  
Author(s):  
Mohammad Alam ◽  
Md. Shahrear Biozid ◽  
Md. Faruk ◽  
Md. Jainul Abeden ◽  
Khandoker Usran Ferdous ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Flowering Plant ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Anti Oxidant

Abstract Background: Nymphaea capensis is an aquatic flowering plant which is included in the family of Nymphaeaceae. Literature review on the plants of Nymphaeaceae family exhibited significant medicinal activities. Therefore, the objective of the present study is to evaluate possible anti-oxidant activity of crude methanol extract of N. capensis leaf.Method: In antioxidant study, methanolic plant extract was evaluated for 1,1-diphenyl,2-picrylhydrazyl (DPPH) and reducing power capacity. Moreover, total phenolic and total flavonoid content of plant extracts were determined and expressed in mg of gallic acid equivalent per gram of dry sample (mg GAE/g dry weight).Result: In the DPPH free radical scavenging assay, methanol extract showed concentration dependent inhibition of the free radicals. IC50 of Ascorbic acid was 14.84 µg/ml whereas N. capensis was 130.94 µg/ml. In case of reducing capacity, at conc. 62.5, 125, 250, 500, 1000 ug/ml, the absorbances of Ascorbic acid were 0.65, 1.12, 1.45, 1.78 and 1.89 respectively. In case of N. capensis, the absorbances were 0.46, 0.75, 1.04, 1.27 and 1.50 respectively. The extract displayed a concentration dependent increase in reducing power. In the case of total phenolic content, the methanol extract of N. capensis contained a considerable amount of phenolic contents which was 215±7 of GAE mg/g. In the case of total flavonoid content, methanol extract of N. capensis contained significant amount of flavonoid contents which was 184.75±6.78 of GAE mg/g.Conclusion: These results suggested that the methanol extract of N. capensis possess considerable anti-oxidant activity.

COMPARATIVE STUDY OF TOTAL PHENOLIC CONTENT, ANTI-OXIDANT ACTIVITY AND FREE RADICALSCAVENGING POTENTIAL OF TWO DIFFERENT SOLVENT EXTRACTS OF MOMORDICA DIOICA ROXB.EX WILD. & MOMORDICA CHARANTIA L. (CUCURBITACEAE)

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (10) ◽  
pp. 33-41
Author(s):  
M. S Kale ◽  
◽  
S. D. Katekhaye
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Momordica Charantia ◽  
In Vitro Assays ◽  
Total Phenolic ◽  
Anti Oxidant ◽  
Acetone Extracts

The fruits of Momordica dioica Roxb. ex Wild. & Momordica charantia L. (Cucurbitaceae) are used as vegetable in India. The fruits are used in the management of diabetes and other diseases. Since these effects may be correlated with the presence of anti-oxidant compounds, methanol and 70% acetone extracts of these plants were evaluated and compared for their total phenolic content, anti-oxidant activity and radical scavenging activity. Total phenolic content of methanol extract of both fruits was found to be relatively higher than acetone extract. The anti-oxidant potential of the extracts were assessed by employing different in vitro assays such as reducing power assay, DPPH, hydrogen peroxide scavenging assay, OH radical scavenging capacities, peroxidation inhibiting activity through linoleic acid emulsion system and anti-hemolytic assay. Though all the extracts exhibited dose dependent reducing power activity, acetone extracts of the samples were found to have more hydrogen donating ability.

Hepatoprotective and Anti-oxidant Activities ofGlossogyne tenuifoliaAgainst Acetaminophen-Induced Hepatotoxicity in Mice

2014 ◽  
Vol 42 (06) ◽  
pp. 1385-1398 ◽  
Author(s):  
Yu-Hsiu Tien ◽  
Bing-Huei Chen ◽  
Guoo-Shyng Wang Hsu ◽  
Wan-Teng Lin ◽  
Jui-Hua Huang ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Serum Levels ◽  
Oxidative Capacity ◽  
Positive Control ◽  
Hot Water ◽  
Total Phenolic ◽  
Control Group ◽  
Anti Oxidant

The present study investigated the anti-oxidative and hepatoprotective effects of Glossogyne tenuifolia (GT) Cassini, against acetaminophen-induced acute liver injury in BALB/c mice. The extracts of GT by various solvents (hot water, 50% ethanol and 95% ethanol) were compared for their 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, reducing power, total phenolic content, and total anti-oxidant capacity. The results showed that hot water (HW) extracts of GT contained high levels of phenolics and exerted an excellent anti-oxidative capacity; thus, these were used in the animal experiment. The male BALB/c mice were randomly divided into control group, acetaminophen (APAP) group, positive control group and two GT groups at low (GT-L) and high (GT-H) dosages. The results showed that mice treated with GT had significantly decreased serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). GT-H increased glutathione levels and the ratios of reduced glutathione and oxidized glutathione (GSH/GSSG) in the liver, and inhibited serum and lipid peroxidation. This experiment was the first to determine phenolic compounds, chlorogenic acid and luteolin-7-glucoside in HW extract of GT. In conclusion, HW extract of GT may have potential anti-oxidant capacity and show hepatoprotective capacities in APAP-induced liver damaged mice.

In Vitro Antioxidant, Anti-Inflammatory And Anti-Microbial Activity of Carica Papaya Seeds

2020 ◽  
pp. 19-38 ◽  
Author(s):  
Pooja G. Singh ◽  
Madhu S. B ◽  
Shailasreesekhar Shailasreesekhar ◽  
Gopenath TS ◽  
Kanthesh M. Basalingappa ◽  
...  
Keyword(s):  
Carica Papaya ◽  
Therapeutic Potential ◽  
Reducing Power ◽  
In Vitro Study ◽  
Antioxidant Property ◽  
Total Phenolic ◽  
Dpph Method ◽  
Papaya Seeds ◽  
Anti Inflammatory

Papaya seeds are reported to have higher therapeutic potential in comparison to the fruits in which they reside. Thus, the present in-vitro study aimed to evaluate and compare the anti-oxidant, anti-inflammatory and anti-microbial effect of seed extracts on Carica papaya L. (Caricaceae). The bioactive form the seeds were sequentially fractionated with hexane, chloroform, diethyl ether, and methanol in the increasing order of polarity. Total phenolic and flavonoid contents were estimated. These extracts were assessed for an antioxidant property by 1, 1-diphenyl-2-picryl-hydroxyl (DPPH) method and reducing power assay was carried out using the FeCl3 method. Inhibition of 15-lipoxygenase (LOX) by these extracts at 5 -25μg to asses anti-inflammatory capacity was studied.

scholarly journals In vitro Antioxidant Properties of Bersama abyssinica Stem Bark Extracts

Proceedings ◽  
2019 ◽  
Vol 40 (1) ◽  
pp. 21
Author(s):  
Kouadio Ibrahime Sinan ◽  
Kouadio Bene ◽  
Abdurrahman Aktumsek ◽  
Gokhan Zengin
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Water Extract ◽  
Reducing Power ◽  
Stem Bark ◽  
Sub Saharan Africa ◽  
Total Phenolic ◽  
Bersama Abyssinica

Bersama abyssinica, belonging to the Melianthaceae family, is distributed across Sub Saharan Africa. Decoctions of the bark, leaves, and roots of B. abyssinica have been extensively used in traditional medicine to manage many stomach complications such as colic, diarrhea, dysentery, and intestinal worms. In this study, we examined three extracts (ethyl acetate, methanol and water) obtained from B. abyssinica stem barks in terms of antioxidant properties. The antioxidant abilities were investigated by different chemical methods, including free radical scavenging (DPPH and ABTS), reducing power (CUPRAC and FRAP), metal chelating and phosphomolybdenum assay. In addition, total phenolic and flavonoid contents in the extracts were calculated. The highest level of phenolics was determined in water extract (230.83 mg GAE/g extract), followed by methanol (216.79 mg GAE/g extract) and ethyl acetate (100.57 mg GAE/g extract). In same line with total phenolic content, the best antioxidant properties were noted for water and methanol extracts. Our findings suggested that B. abyssinica stem bark extracts could be considered as promising sources of natural antioxidants.

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