scholarly journals PREVENTIVE EFFECT OF PHASEOLUS VULGARIS SEED COATS ON PENTYLENETETRAZOLE (PTZ) INDUCED KINDLING AND BEHAVIORAL COMORBIDITIES

2021 ◽  
Vol 3 (4) ◽  
pp. 20-25
Author(s):  
Sarika Chaudhary ◽  
Sagarika Majhi ◽  
Madhu Verma

Introduction: To investigate the preventive effect of Phaseolus vulgaris seed coats on pentylenetetrazole-induced kindling and behavioral comorbidities. Material and Methods: Thirty Wistar albino rats were categorized into five groups. The first group received regular saline (0.9 % w/v NaCl) p.o.; the second group received PTZ (35 mg/kg b.w.) i.p.; the third group received valproic acid (200 mg/kg b.w.) p.o.; the fourth group received P. vulgaris extract (100 mg/kg b.w.) p.o.; the fifth group received P. vulgaris extract (200 mg/kg b.w.) p.o. on an alternate day for 21 days. PTZ improved lipid peroxidase levels, decreased Glutathione level, decreased superoxide dismutase activity, increased Nitric Oxide level. Result: This study revealed that P. vulgaris (Hydroalcoholic extract) increased the anti-oxidant level of both 100 mg/kg and 200 mg/kg compared to the PTZ category. Histopathological findings revealed that the hippocampal section of the brain of rats receiving P. vulgaris extract had improved relative to the receiving PTZ group. Conclusion: Based on the result, it is proposed that Phaseolus vulgaris has anti-oxidant properties. This is useful for the treatment of epilepsy.

2010 ◽  
Vol 105 (4) ◽  
pp. 517-525 ◽  
Author(s):  
María J. M. Alférez ◽  
Javier Díaz-Castro ◽  
Inmaculada López-Aliaga ◽  
María Rodríguez-Ferrer ◽  
Luis Javier Pérez-Sánchez ◽  
...  

Despite Fe deficiency having been widely studied, the sequence of events in its development still remains unclear. The aim of the present study was to elucidate the effects of nutritional Fe-deficiency development on haematological parameters, Fe bioavailability and the enzymes involved in oxidative defence in recently weaned male Wistar albino rats. Control (C) and Fe-deficient (ID) groups were fed the AIN-93 G diet with a normal Fe level (45 mg/kg diet) or with a low Fe level (5 mg/kg diet), respectively, for 20, 30 or 40 d. At day 20 serum Fe, serum ferritin and the saturation of transferrin decreased drastically, decreasing further in the course of Fe-deficiency development for the saturation of transferrin. The development of Fe deficiency did not affect plasma thiobarbituric acid-reactive substance production, or catalase (CAT) and glutathione peroxidase (GPx) activities in erythrocyte cytosol. Fe deficiency diminished hepatic Fe content and CAT and GPx activities in hepatic cytosol only at day the 20. However, in spite of the minor Fe deposits in the brain of ID rats, the CAT and GPx activities in the brain cytosolic fraction did not differ in any of the studied periods v. control rats. These results show that brain is a tissue that does not seem to depend on Fe levels for the maintenance of antioxidant defence mechanisms in the course of nutritional Fe deficiency.


2016 ◽  
Vol 4 (6) ◽  
pp. 276-281
Author(s):  
Desai Yogesh Hareshchandra ◽  
◽  
Ravi Mundugaru ◽  
Shridhara Bairy T ◽  
S Ravikrishna ◽  
...  

Mucuna pruriens Bek. and Mucuna monosperma DC. seeds were extensively used in Ayurveda for neuromuscular disorders. The objective of present study was to screen the neuroprotective activity of the test drugs in wistar albino rats. The cerebral ischemia was induced by bilateral common carotid artery occlusion for 60 minutes followed by reperfusion. At the end of the experiments under anaesthesia animals were sacrificed and brain was removed. Anti oxidant and histopathological examination was carried out of the brain tissue. Both test drugs have been shown considerable anti oxidant activity in comparison to BCCAO control group. Histopathological examination revealed there is a decreased cellularity and predominance of immature neurons in the granular layer was observed in hypothalamus in control rats while the test drug has shown normal cytoarchitecture. The sections of hippocampus from Mucuna monosperma DC group exhibited decreased cellularity of the granular layer and vacuolization was observed and Mucuna pruriens Bek treated group exhibited almost normal cytoarchitecture. In conclusion the test drugs possess moderate anti oxidant and cellular integrity maintaining potential in different brain regions and hence it supports its therapeutic claim in neuromuscular disorders.


2021 ◽  
Vol 1 (1) ◽  
pp. 1-7
Author(s):  
Jonathan C. Ifemeje ◽  
◽  
Chukwuebuka Egbuna ◽  
Chukwuemelie Zedech Uche ◽  
Michael Chinedu Olisah ◽  
...  

The chemical composition and the effects of four local species of beans on the liver and kidney function of Wistar albino rats were examined. The four beans species are Cajanus Cajan, Vigna unguiculata subsp. sesquipedalis, Phaseolus vulgaris L ‘Red kidney’, and Phaseolus vulgaris 'Black turtle’. The results obtained for the proximate composition analysis revealed that the protein content of the bean species was higher in P. vulgaris L ‘Red kidney’ (18.54±0.01a %), and P. vulgaris 'Black turtle’ (18.36±0.01a %) with no significant difference at P< 0.05. The phytochemical composition analysis revealed that the beans contain various phytochemicals including some anti-nutritional factors. The mineral composition analysis shows that V. u. subsp. Sesquipedalis had a higher level of minerals (4.80±4.43a mg/100g) followed by C. cajan (3.24±2.64b mg/100g). The experimental design comprises of 30 male Wistar albino rats distributed into 5 groups of 6 rats each. Each group except the control received 50g of normal rat feed + 100g of beans body weight. The effects of the beans species on the biochemical parameters suggest no adverse effects when compared to the control. Despite that, these beans contain high nutritional components that are of nutritional interest as as well as some anti-nutritional factors.


INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (07) ◽  
pp. 46-52
Author(s):  
Sreedevi Adikay ◽  
Sai Sruthi Kaveripakam ◽  

The gravity of the impact of drug induced nephrotoxicity is more prominent in society. The present study was undertaken to evaluate the potential of hydroalcoholic extract of seeds of Sorghum bicolor against cisplatin and doxorubicin- induced nephrotoxicity in Wistar albino rats. The nephrotoxicity was modeled by intraperitoneal injection of cisplatin (5 mg/kg b.w.) in cisplatin model and doxorubicin (15 mg/kg b.w.) in doxorubicin-induced model in rats. Nephroprotection of hydroalcoholic extract of seeds of S. bicolor was evaluated at two different doses of 200 and 400mg/kg b.w. The nephroprotective activity was assessed by the determination of various serum and urinary parameters, anti-oxidant studies, histological and immunohistochemical studies. The results indicated that injection of cisplatin and doxorubicin led to marked nephrotoxicity in animals. Treatment with extract in cisplatin-induced model resulted in significant nephroprotective activity in a curative regimen whereas in prophylactic regimen the extract prevented the induction of nephrotoxicity only up to a considerable level. But the extract failed to attenuate the doxorubicin induced nephrotoxicity, as evident by biochemical, histological and immunohistochemical studies. From the findings, it is concluded that the seeds of S. bicolor can be used as a novel approach in the treatment of cisplatin-induced nephrotoxicity.


Author(s):  
Ravindra Reddy Kandula ◽  
Narasimha Jayaveera Korlakunta ◽  
Raghavendra Mitta

  Objective: The objective of the present study was to explore the in vitro antioxidant and effect of hydroalcoholic extract of Arthrospira platensis (ASP) against sodium fluoride (NaF)-induced toxic effects on soft tissues (heart, liver, and kidney).Methods: In vitro antioxidant activity was assessed using 1,1-diphenyl-2-picrylhydrazyl radical scavenging, 2,2’-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid) radical scavenging, metal chelation, total antioxidant and reducing power assays, and total flavonoid and phenol content. In this study, 36 male Wistar albino rats were divided into six groups of six animals each. Animals were served as Group I - normal control, Group II - toxic control, and Groups III, IV, and V - treatment groups, which received hydroalcoholic extract of ASP at doses of 100, 200, and 400 mg/kg body weight (p.o), respectively. Group VI served as plant control received hydroalcoholic extract of ASP at a dose of 400 mg/kg body weight (p.o). All groups except Groups I and VI received NaF (100 ppm) through drinking water for 30 days.Results: Results showed that intoxication of NaF caused significant elevation of serum biomarkers of the heart, liver, and kidney and altered tissue oxidative stress markers’ levels. Administration of hydroalcoholic extract of ASP significantly normalized elevated serum levels of heart (p<0.001), liver (p<0.001, p<0.05), and kidney biomarkers (p<0.001). Decrease in lipid peroxidation and increase in reduced glutathione and catalase enzyme levels in a dose-dependent manner were observed in soft tissues (p<0.001, p<0.01, and p<0.05).Conclusion: The study revealed that ASP has good antioxidant and mitigative action against NaF intoxication on soft tissues.


Author(s):  
Malathi S. ◽  
Vidyashree . ◽  
Ravindran Rajan

Objective: To identify the memory enhancing role of Michelia champaca in acute noise stressed animals. Methods: Male Wistar albino rats were used in this study. Animals were exposed to noise for 4 h before testing for memory. Thereafter, the plasma corticosterone level and acetylcholinesterase activity were estimated in the discrete regions of the brain, and the memory related behavior were assessed by eight arm radial maze.Results: Our results showed that Michelia champaca enhances the memory activity and decreases the corticosterone concentrations in acute noise stress animals treated with M. champaca. Moreover, it also decreased brain acetylcholinesterase activity when compared with the acute stress group (p<0.05). Furthermore, behavioral tests indicate that working memory, is enhanced by acute stress and decreases the error levels in all the parameters studied in the behavior aspects when compared to control animals.Conclusion: These findings suggest that Michelia champaca enhances the memory in albino rats and might be useful therapeutically for cognitive related dysfunctions. This could be due to the presence of memory boosting compounds and its antistressor and anti-acetylcholinesterase activity, thereby reduces the levels of serum corticosterone and inhibition of cholinesterase enzyme significantly.


2020 ◽  
Vol 15 (6) ◽  
pp. 401-412
Author(s):  
Murat Karamese ◽  
Hakan Aydin ◽  
Volkan Gelen ◽  
Emin Sengul ◽  
Selina Aksak Karamese

Aim: The objective of this study was to evaluate the possible protective effects of probiotic bacteria, especially Bifidobacterium and Lactobacillus strains, on 4,4′-dichlorodiphenyltrichloroethane (DDT)-induced toxicity. For this reason, we evaluated the relationship between probiotics and toxicity by checking immunological and immunohistochemical parameters. Materials & methods: Probiotic pretreatment was applied to 36 Wistar albino rats for 12 consecutive days. Serum aspartate aminotransferase and alanine aminotransferase levels were detected. CD3 and NF-κB staining methods were then performed by immunohistochemistry. Finally, pro- and anti-inflammatory cytokines were measured by ELISA. Results: DDT caused a serious increase/decrease in some cytokine parameters. The effective dose was 1 × 1011 colony-forming unit probiotic treatment. CD3 and NF-κB positivity were intense in DDT group whereas the intensity was reduced in probiotic treatment groups. Discussion: The probiotic mixture has a potential to prevent inflammatory and oxidative stress related organ injuries. Further studies should be performed to explain the possible mechanisms.


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