scholarly journals Antioxidant Properties of the Leaves of Chromolaena odorata Linn

2013 ◽  
Vol 35 (1) ◽  
pp. 53-57
Author(s):  
D Bhargava ◽  
CK Mondal ◽  
JN Shivapuri ◽  
S Mondal ◽  
S Kar
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Rational Approach ◽  
Chromolaena Odorata

 Introduction: Chromolaena odorata Linn (Asteraceae) locally known as “Banmara” has the reputation of being used as a medicinal herb in Nepal. A systemic search for drug research in now considered to be a rational approach. Therefore in the present study an effort has been made to evaluate the antioxidant activity of the leaves of the above mentioned plant. Methods: In the present research work the ethanolic and methanolic extract of leaves of the plant C. odorata Linn was assessed for free radical scavenging and antioxidant activity. In this work the ability of the extract to scavenge nitric oxide, hydroxyl radical and 1,1-diphenyl-2- picrylhydrazyl (DPPH) were used to assess its free radical scavenging potentials. Results: The DPPH radical inhibition (%) was 59.10, 52.13 and 81.12 for ethanolic extract, methanolic extract and ascorbic acid respectively. Similarly ethanolic and methanolic extract  also showed significant free radical scavenging action against nitric oxide and hydroxyl radical. Conclusion: Against the backdrop of many known medicinal properties of this plant its ethanolic and methanolic extract of leaves reveals significant antioxidant properties. DOI: http://dx.doi.org/10.2126/joim.v35i1.8900   Journal of Institute of Medicine, April, 2013; 35:53-57

Evaluation of antioxidant activity of Melothria maderaspatana in vitro

2010 ◽  
Vol 5 (2) ◽  
pp. 224-230 ◽  
Author(s):  
Boobalan Raja ◽  
Kodukkur Pugalendi
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Superoxide Anion ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Melothria Maderaspatana

AbstractIn this study, an aqueous extract of leaves from Melothria maderaspatana was tested for in vitro antioxidant activity. Free radical scavenging assays, such as hydroxyl radical, hydrogen peroxide, superoxide anion radical and 2,2-diphenyl-1-picryl hydrazyl (DPPH), 2,2’-azinobis-(3-ethyl-enzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and reducing power assay, were studied. The extract effectively scavenged hydroxyl radical, hydrogen peroxide and superoxide anion radicals. It also scavenged DPPH and ABTS radicals. Furthermore, it was found to have reducing power. All concentrations of leaf extract exhibited free radical scavenging and antioxidant power, and the preventive effects were in a dose-dependent manner. The antioxidant activities of the above were compared to standard antioxidants such as butylated hydroxytoluene (BHT), ascorbic acid, and α-tocopherol. The results obtained in the present study indicate that the M. maderaspatana extract could be considered a potential source of natural antioxidant.

scholarly journals Domestic Cooking Effects of Bambara Groundnuts and Common Beans in the Antioxidant Properties and Polyphenol Profiles

2017 ◽  
Vol 6 (2) ◽  
pp. 24 ◽  
Author(s):  
V. Nyau ◽  
S. Prakash ◽  
J. Rodrigues ◽  
J. Farrant
Keyword(s):  
Free Radical ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Esi Ms ◽  
Positive Effects ◽  
Phytochemical Profiles

Processing of legumes before consumption has several effects on micronutrients, macronutrients and phytonutrients. This study was undertaken to investigate the effect of domestic processing on antioxidant activities and phenolic phytochemicals of the red bambara groundnuts and red beans. The study employed in vitro antioxidant assays (DPPH and FRAP) to screen for antioxidant properties, HPLC-PDA-ESI-MS and Folin Ciocalteu assay to screen for phenolic phytochemical profiles. Domestic cooking displayed positive effects on the antioxidant activity and phenolic phytochemical profiles of the two legumes. The free radical scavenging speed increased 10-fold in the methanolic extract from cooked red bambara groundnuts compared to uncooked. By contrast, the free radical scavenging speed increased 20-fold in the methanolic extract from cooked red beans compared to uncooked. HPLC-PDA-ESI-MS profiles of the cooked red bambara groundnuts and red beans revealed a number of emergent phenolic compounds, mainly flavonoids. These data indicate that cooking appear to enhance the nutraceutical profiles of the legumes investigated.

scholarly journals Phytochemical Composition and Antioxidant Potential of Ruta graveolens L. In Vitro Culture Lines

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Renuka Diwan ◽  
Amit Shinde ◽  
Nutan Malpathak
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Ruta Graveolens ◽  
Oxide Reduction ◽  
Nitric Oxide Reduction

Ruta graveolens L. is a medicinal plant used in traditional systems of medicine for treatment of psoriasis, vitiligo, leucoderma, and lymphomas with well-known anti-inflammatory and anticancer properties. Therefore antioxidant potential of R. graveolens (in planta and in vitro) was investigated. As antioxidants present in plant extracts are multifunctional, their activity and mechanism depends on the composition and conditions of the test system. Therefore, the total antioxidant capacity was evaluated using assays that detect different antioxidants: free radical scavenging (DPPH and ABTS), transition metal ion reduction (phosphomolybdenum assay), reducing power, and nitric oxide reduction. Content of furanocoumarin-bergapten in the extracts showed good corelation with free radical scavenging, transition metal reduction and reducing power, while total phenolic content showed good corelation with nitric oxide reduction potential. Antioxidant activity of in vitro cultures was significantly higher compared to in vivo plant material. The present study is the first report on comprehensive study of antioxidant activity of R. graveolens and its in vitro cultures.

scholarly journals ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF LEUCAS ASPERA FLOWERS FROM BIHAR, INDIA.

2018 ◽  
Vol 11 (2) ◽  
pp. 223
Author(s):  
Gulnaaz Sabri ◽  
Vimala Y
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Free Radical ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Free Radical Scavenging Assay

 Objective: The aim of this study was to explicate antibacterial, antifungal, and antioxidant activities of Leucas aspera flowers.Methods: Antibacterial activity was done by agar diffusion method. The ethyl acetate extract of L. aspera flower was evaluated against both Gram-positive and Gram-negative bacteria. Antifungal activity was also done by agar diffusion method. The agar used for antifungal activity was Czapek Dox Agar. Nitric oxide scavenging assay and free radical scavenging assay were used for the antioxidant activity. Griess reagent was used in nitric oxide scavenging assay. 1,1-diphenyl-2-picryl hydrazyl was used in free radical scavenging assay.Results: L. aspera flower extract showed good antibacterial activity with the highest zone of inhibition against Vibrio cholera with 23 mm followed by Bacillus polymyxa showing 20 mm zone of inhibition. The ethyl acetate extract of L. aspera flower showed quite a good results with the highest inhibitory activity against Aspergillus niger with 13 mm zone of inhibition and lowest for Trichoderma viridae with 5 mm zone of inhibition. Antioxidant activity of L. aspera flower extract was done by free radical scavenging assay and nitric oxide scavenging assay. Nitric oxide scavenging assay showed prominent results almost performed equal to standard compound Butylated hydroxyl anisole (BHA) The values for 10 μl of L. aspera extract was 50.27, for the standard (BHA) showed 50.81. L. aspera extract values for 50 μl was 69.73 and for BHA, the values was 77.30. For 100 μl, the extract gave 82.70, and for standard BHA, the reading was 89.73.Conclusion: The results showed that L. aspera flower has broad-spectrum antibacterial activity ranging from 23 to 13 mm zone of inhibition. L. aspera flower has strong antioxidative power on nitric oxide radicals. The medicinal properties of plant species have made an outstanding contribution to the origin and evolution of many traditional herbal therapies.

scholarly journals IN VITRO EVALUATION OF ENZYMIC ANTIOXIDANTS AND FREE RADICAL SCAVENGING ACTIVITY IN THE ETHANOLIC EXTRACT OF CRESCENTIA CUJETE LEAVES

2020 ◽  
pp. 92-95
Author(s):  
ANITHA P ◽  
NAZEEMA TH
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Glutathione Reductase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Enzymic Antioxidants

Objectives: This study aims to evaluate the enzymic antioxidants and free radical scavenging present in the ethanolic leaf extracts of Crescentia cujete. Methods: Enzymic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase, glutathione peroxidase, and glutathione S-transferase (GST) were estimated by standard methods. Free radical scavenging potential was evaluated by diphenylpicrylhydrazyl (DPPH), nitric oxide, and hydroxyl radical methods using an ethanolic extract of C. cujete leaf. Results: The leaf extract of C. cujete showed the maximum activity of CAT, SOD, GST, glutathione reductase, and peroxidase activity. CAT activity was formed to be highest in the ethanolic extract of C. cujete leaf. DPPH radical scavenging activity was reported as 38.5 μg/ml, nitric oxide was found to be 200.77 μg/ml, and hydroxyl radical scavenging exhibited 108.42 μg/ml normalized with ascorbic acid. Conclusion: From the results, it has concluded that the ethanol extract of the C. cujete leaf has a prospective source of natural antioxidant that would be a great significance as therapeutic agents in preventing or slowing the progress of reactive oxygen species and related oxidative stress-related degenerative diseases.

scholarly journals ANTIOXIDATIVE AND FREE RADICAL SCAVENGING POTENTIALS OF HABENARIA PECTINATA

2020 ◽  
pp. 68-71
Author(s):  
CHARANJIT KAUR ◽  
RAJESH KUMAR ◽  
GURVINDER SINGH ◽  
CHANDER MOHAN
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Antioxidant Potential ◽  
Flavonoid Content ◽  
Anti Oxidant

Objective: This study was designed to evaluate the antioxidative potential of tubers of Habenaria pectinata. Methods: The tubers of H. pectinata were extracted using hexane, ethyl acetate, methanol, and water as solvents. The anti-oxidant potential of extracts was evaluated using free radical scavenging and reducing power assays. The most active methanolic extract was then fractionated into four fractions using the above-mentioned solvents. Results: The phenol and flavonoid content was found to be maximum in the methanol extracts. All the extracts and fractions showed significant levels of antioxidant activity except hexane extract. Conclusion: The tubers of H. pectinata were found to possess a significant antioxidant potential and can be explored further for isolation and preclinical investigation for the ailment of various diseased states and disorders.

scholarly journals COLLECTION, IDENTIFICATION, MOLECULAR CHARACTERIZATION, AND ANTIOXIDANT ACTIVITY OF NON-GILLED MUSHROOMS COLLECTED FROM NORTH WESTERN HIMALAYAS

2018 ◽  
Vol 11 (10) ◽  
pp. 254
Author(s):  
Shaveta Singh ◽  
Astha Tripathi
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Molecular Taxonomy ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Morphological Identification ◽  
Western Himalayas ◽  
Wide Range ◽  
North Western

Objective: The objective of present research deals with collection, taxonomic, molecular taxonomy characterization, and antioxidant activity of wild non-gilled mushrooms sample collected from North Western Himalayas.Methods: The wild non-gilled mushroom samples were characterized for micro, morphological properties, and molecular identification. 1,1 diphenylpicrylhydrazyl (DPPH), nitric oxide (NO2), hydrogen peroxide (H2O2), and free radical scavenging assay were used to evaluate the antioxidant properties of selected wild non-gilled mushrooms.Results: The obtained results revealed that on the basis of microscopic and morphological identification all the four non-gilled mushrooms samples were tentatively identified as 2/15 (Auricularia sp.1), 6/15 (Auricularia sp.2), 32/15 (Ganoderma sp.), and 132/15 (Trametes sp.). The colony, mycelial, and basidiospore morphology of fungal isolate were evidence of Basidiomycetes family. All these four non-gilled mushrooms were sequenced. Sequences were submitted to National Center for Biotechnology Information (NCBI), and their accession number was MF770159 (2/15), MF774107 (6/15), MF770158 (32/15), and MF770160 (132/15). The phylogenetic analysis of 18S ribosomal deoxyribonucleic acid (18S rDNA) sequence of four non-gilled mushrooms was compared with other species and identified as Auricularia polytricha, Ganoderma lucidum, Auricularia auricula-judae, and Trametes elegans. The four non-gilled mushrooms extract exhibited DPPH, NO2, and H2O2 free radical scavenging activities. G. lucidum methanolic extract has the highest effect on free radicals in comparison to hexane effect.Conclusion: All the isolated four non-gilled mushrooms showed good antioxidant potential, and it can be concluded that these mushrooms are not only consumed but also have a wide range of medicinal properties which must further explode for future use.

scholarly journals Evaluation of Free Radical Scavenging Potential of Different Bioactive Fractions Present in Boerhavia diffusa Linn. Root Extract: An in-vitro Approach

2020 ◽  
pp. 99-107
Author(s):  
Mohammad Khalid ◽  
Mohammed H. Alqarni ◽  
Ahmed I. Foudah ◽  
Juber Akhtar ◽  
Badruddeen . ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Chloroform Fraction ◽  
Scavenging Activity ◽  
Boerhavia Diffusa

Aim and Objectives: Boerhavia diffusa (B. diffusa) frequently known as punarnava is specifically used to replenish the body. The present work was designed to evaluate the scavenging potential of its bioactive constituents. Materials and Methods: The different fractions of B. diffusa root methanolic extract were examined for phenolic, flavonoids contents, DPPH free radical and Nitric oxide scavenging activities. Further antioxidant activity was evaluated by ABTS free radical scavenging method and also from the reducing potential scavenging activity. The total phenolic content in different fractions by using various solvent like ethanol, diethyl ether, chloroform, ethyl acetate, and n-butanol were carried out to find the antioxidant activities. Results: The phenolic content was highest in ethanolic fraction that was significantly comparable with ascorbic acid. The flavonoid content was highest in ethanol fraction (41.93 ± 3.92 µg/mL) followed by n-butanol fraction (31.68 ± 1.72 µg/mL), then ethyl acetate fraction (29.67 ± 2.83 µg/mL) and least in chloroform fraction (16.91 ± 2.74 µg/mL). The ethanolic fraction of B. diffusa also showed highest DPPH radical scavenging activity (101.29 ± 3.78) when compared with other fractions of same extract using different solvent phases. Moreover the nitric oxide scavenging activity of ethanolic fraction was maximum (82.31 ± 2.83) than different fractions. The ethanolic fraction also showed improved ABTS radical scavenging activity (81.73 ± 2.73 mg/mL) while chloroform fraction showed poor ABTS radical cation scavenging activity (29.51 ± 2.79 mg/mL). Conclusions: The study concludes that Boerhavia diffusa has rich and ample source of phenolic acid and flavonoids. Among all fractions the ethanolic has potent antioxidant activity, which shows its significance for a better novel approach.

scholarly journals Antioxidant Screening on the Whole Plant of Cyanthillium cinereum (L) H.Rob

2021 ◽  
Vol 8 (7) ◽  
pp. 458-461
Author(s):  
Mohammed Shihab K. K. ◽  
Rajagopal P. L. ◽  
Nasila K.
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Whole Plant

Methanolic extract of the whole plant of Cyanthillium cinereum (L) H.Robwasevaluated for free radical scavenging activity by using Nitric oxide scavenging activity (NO) and Reduction of 1, 1-Diphenyl -2-Picryl Hydrazyl (DPPH) free radical scavenging activity. Throughout the studies the plant extract showed potent antioxidant activity and the activity was found to be concentration dependent which may be attributed to the high flavonoid content of the plant. Keywords: Cyanthillium cinereum (L) H.Rob, DPPH, NO.

scholarly journals Phytochemical Analysis and Antimicrobial/Antioxidant Activity of Cynodon dactylon (L.) Pers. Rhizome Methanolic Extract

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Samira Savadi ◽  
Mohsen Vazifedoost ◽  
Zohre Didar ◽  
Mohammad Mahdi Nematshahi ◽  
Eisa Jahed
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Cynodon Dactylon ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Inhibition Zone ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity

A proper use of medicinal plants requires accurate scientific information and understanding of their chemical constituents. The therapeutic effects in the plants are due to the chemical compounds therein. Cynodon dactylon (L.) Pers. of the family Poaceae is a perennial, pan-tropical species of grass which is a well-known traditional medicine and has a renowned position for treatment of many symptoms and diseases. The chemical composition, free radical scavenging activity, and antimicrobial properties of the methanolic extract of C. dactylon rhizomes against selected bacterial and fungal strains were investigated using disk-diffusion method. The results indicated that the major fatty acid structures of C. dactylon methanolic extract were palmitic acid (36.40%), oleic acid (28.26%), and linoleic acid (17.01%). Alpha-tocopherol (151.39 mg·kg−1) and sitosterol (3199.62 mg·kg−1) were the main tocopherols and sterols, respectively. According to the instrumental analysis, the total phenolic compounds of methanolic extract were equal to 917.08 mg·kg−1 and the main compound was hydroquinone (66.89%). Antioxidant activity of the methanolic extract at concentrations of 100–1000 ppm was 9.81–67.87%, which is significantly different from the 200 ppm synthetic antioxidant (BHT) with free radical scavenging activity equal to 48.93% (p<0.05). The antimicrobial study revealed that the methanolic extract of C. dactylon rhizomes was effective against all of the bacterial pathogens and the antibacterial activity increased with an increase in the concentration of extract. Therefore, the largest zone of inhibition was observed against Bacillus cereus (18.3 ± 0.4 mm) and Escherichia coli (16.8 ± 0.5 mm) at 1000 ppm. The highest resistance was observed with Pseudomonas aeruginosa (inhibition zone of 12.8 ± 0.15 mm). Methanolic extract at 200 ppm had no effect against fungus Aspergillus niger. However, at 1000 ppm concentration, an inhibition zone with a diameter of 14.4 ± 0.45 mm was formed.

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