scholarly journals Assessment of Knowledge and Attitude Among College Students Toward Umbilical Cord Blood and its Banking

2021 ◽  
Vol 1 (1) ◽  
pp. 1-7
Author(s):  
Namita Yadav ◽  
Vita Leena D'Souza ◽  
T. Geethamani

Introduction: Umbilical cord blood (UCB) is a good source of haematopoietic stem cells and progenitor cells. UCB stem cells are used in treatment of a variety of blood and bone marrow diseases, blood cancers, metabolic disorders and immune deficiencies. Objective: This study aimed in investigating college students’ knowledge and attitude about UCB and UCB banking. Methods: It is a descriptive cross-sectional study. The study was conducted in East West College of Management, Bangalore, India from September 2018 to August 2019. A total 80 college students were selected by random sampling techniques. There were self-structured questionnaires used to assess the knowledge and attitude regarding UCB and UCB banking. The data analysis was done using descriptive and inferential statistics in SPSS version 21. Results: The maximum number of participants were from age group 19-21 years followed by age group 16-18 years. The overall knowledge score was 21.05±5.11 with mean percentage score of 60.14. The 71.25% (57) of subjects had moderately adequate knowledge; whereas, 25% (20) of subjects showed adequate knowledge. Similarly, the overall attitude score of subjects was 61.5±5.17 with a mean percentage score of 51.25%. Out of the total participants, 90% (72) had neutral attitude, 10% (8) exhibited positive attitude and none of the subjects exhibited negative attitude towards UCB banking. There was no association found between knowledge and sociodemographic variables and between attitude and sociodemographic variables. Conclusion: The majority of the respondents had moderate knowledge and maximum had neutral atti­tude on umbilical cord blood and umbilical cord blood banking.

Blood ◽  
2000 ◽  
Vol 96 (6) ◽  
pp. 2125-2133 ◽  
Author(s):  
Robert W. Storms ◽  
Margaret A. Goodell ◽  
Alan Fisher ◽  
Richard C. Mulligan ◽  
Clay Smith

Abstract A novel Hoechst 33342 dye efflux assay was recently developed that identifies a population of hematopoietic cells termed side population (SP) cells. In the bone marrow of multiple species, including mice and primates, the SP is composed primarily of CD34−cells, yet has many of the functional properties of hematopoietic stem cells (HSCs). This report characterizes SP cells from human umbilical cord blood (UCB). The SP in unfractionated UCB was enriched for CD34+ cells but also contained a large population of CD34− cells, many of which were mature lymphocytes. SP cells isolated from UCB that had been depleted of lineage-committed cells (Lin− UCB) contained CD34+ and CD34− cells in approximately equivalent proportions. Similar to previous descriptions of human HSCs, the CD34+Lin− SP cells were CD38dimHLA-DRdimThy-1dimCD45RA−CD71−and were enriched for myelo-erythroid precursors. In contrast, the CD34−Lin− SP cells were CD38−HLA-DR−Thy-1−CD71−and failed to generate myelo-erythroid progeny in vitro. The majority of these cells were CD7+CD11b+CD45RA+, as might be expected of early lymphoid cells, but did not express other lymphoid markers. The CD7+CD34−Lin− UCB SP cells did not proliferate in simple suspension cultures but did differentiate into natural killer cells when cultured on stroma with various cytokines. In conclusion, the human Lin− UCB SP contains both CD34+ multipotential stem cells and a novel CD7+CD34−Lin− lymphoid progenitor. This observation adds to the growing body of evidence that CD34− progenitors exist in humans.


Vox Sanguinis ◽  
2013 ◽  
Vol 105 (4) ◽  
pp. 346-354 ◽  
Author(s):  
M. Närhi ◽  
O. Natri ◽  
I. Desbois ◽  
D. Kinggaard Holm ◽  
G. Galea ◽  
...  

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e34899 ◽  
Author(s):  
Ralitza Danova-Alt ◽  
Andreas Heider ◽  
Dietmar Egger ◽  
Michael Cross ◽  
Rüdiger Alt

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