scholarly journals Some characteristics of purpurin-18synthesised from chlorophyll a of Spirulina

2021 ◽  
Vol 63 (11) ◽  
pp. 40-43
Author(s):  
Minh Hieu Nguyen ◽  
◽  
Binh Duong Le ◽  
Anh Tuan Mai ◽  
Thi Binh Nguyen ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Chlorophyll A ◽  
Fluorescence Lifetime ◽  
Acetone Solution ◽  
Research Groups ◽  
Good Dispersion

Research and synthesis of photosensitive purpurin 18 (Pp-18) from nature is one of the topics that many research groups are interested in and developing. In this study, the authors defined some characteristics of Pp-18 synthesised from chlorophyll a - a substance isolated from Spirulina. The results showed that Pp-18 had good dispersion in acetone at 478.5 nm (R2=0.98285) and reached 98%. Fluorescence spectroscopy of Pp-18 in acetone was measured at a concentration of 70 ppm, wavelengths 365.39, 417.62, and 557.96 nm. The fluorescence lifetime of Pp-18 in acetone solution was 2.85 ns.

scholarly journals Fast and Robust 2D Inverse Laplace Transformation of Single-Molecule Fluorescence Lifetime Data

2021 ◽  
Author(s):  
Saurabh Talele ◽  
John T. King
Keyword(s):  
Nmr Spectroscopy ◽  
Fluorescence Spectroscopy ◽  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Conformational Dynamics ◽  
Correlation Spectroscopy ◽  
Great Promise ◽  
Biological Macromolecules ◽  
Single Molecule Fluorescence ◽  
Laplace Transformations

AbstractFluorescence spectroscopy at the single-molecule scale has been indispensable for studying conformational dynamics and rare states of biological macromolecules. Single-molecule 2D-fluorescence lifetime correlation spectroscopy (sm-2D-FLCS) is an emerging technique that holds great promise for the study of protein and nucleic acid dynamics as it 1) resolves conformational dynamics using a single chromophore, 2) measures forward and reverse transitions independently, and 3) has a dynamic window ranging from microseconds to seconds. However, the calculation of a 2D fluorescence relaxation spectrum requires an inverse Laplace transition (ILT), which is an ill-conditioned inversion that must be estimated numerically through a regularized minimization. The current methods for performing ILTs of fluorescence relaxation can be computationally inefficient, sensitive to noise corruption, and difficult to implement. Here, we adopt an approach developed for NMR spectroscopy (T1-T2 relaxometry) to perform 1D and 2D-ILTs on single-molecule fluorescence spectroscopy data using singular-valued decomposition and Tikhonov regularization. This approach provides fast, robust, and easy to implement Laplace inversions of single-molecule fluorescence data.Significance StatementInverse Laplace transformations are a powerful approach for analyzing relaxation data. The inversion computes a relaxation rate spectrum from experimentally measured temporal relaxation, circumventing the need to choose appropriate fitting functions. They are routinely performed in NMR spectroscopy and are becoming increasing used in single-molecule fluorescence experiments. However, as Laplace inversions are ill-conditioned transformations, they must be estimated from regularization algorithms that are often computationally costly and difficult to implement. In this work, we adopt an algorithm first developed for NMR relaxometry to provide fast, robust, and easy to implement 1D and 2D inverse Laplace transformations on single-molecule fluorescence data.

Fluorescence lifetime of chlorophyll a in pure and mixed langmuir-blodgett films

1986 ◽  
Vol 129 (1) ◽  
pp. 41-47 ◽  
Author(s):  
G. Picard ◽  
G. Munger ◽  
R.M. Leblanc ◽  
R. Le Sage ◽  
D. Sharma ◽  
...  
Keyword(s):  
Chlorophyll A ◽  
Fluorescence Lifetime ◽  
Langmuir Blodgett ◽  
Langmuir Blodgett Films

scholarly journals Fluorescence Lifetime Standards for Time and Frequency Domain Fluorescence Spectroscopy

2007 ◽  
Vol 79 (5) ◽  
pp. 2137-2149 ◽  
Author(s):  
Noël Boens ◽  
Wenwu Qin ◽  
Nikola Basarić ◽  
Johan Hofkens ◽  
Marcel Ameloot ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Frequency Domain ◽  
Fluorescence Lifetime

Adsorption of chlorophyll-a from acetone solution on natural and activated bentonite

1994 ◽  
Vol 61 (2) ◽  
pp. 175-178 ◽  
Author(s):  
E. González Pradas ◽  
M. Villafranca Sánchez ◽  
M. Socías Viciana ◽  
A. Gallego Campo
Keyword(s):  
Chlorophyll A ◽  
Acetone Solution ◽  
Activated Bentonite

scholarly journals Efek Beta Karoten dan Agregasi Klorofil Pada Fotostabilitas Klorofil a dalam Pelarut Aseton

2012 ◽  
Vol 11 (2) ◽  
pp. 115
Author(s):  
Junet. F. da Costa ◽  
Ferry F Karwur ◽  
Leenawaty Limantara
Keyword(s):  
Chlorophyll A ◽  
Red Light ◽  
Photosynthetic Pigment ◽  
Beta Carotene ◽  
Acetone Solution ◽  
Aggregate Form ◽  
Color Additive ◽  
The Stability

Chlorophyll as photosynthetic pigment has many benefits to human such as antioxidant, antibacterial, color additive,immunity, and photosensitizer. Concerning to these applications, chlorophyll a will react with oxygen and light andreduce its effectiveness. It is then important to understand the stability of chlorophyll a on oxygen and light.Stability of chlorophyll a in the presence of beta-carotene (1:1) in acetone was studied for various waterconcentrations. Each solution was exposed to red light (ë e” 630 nm) for 0.5; 1.0; 2.5; 5.0; 10; 20; 30; 40; 50; and 60minutes and their spectrum were analysed. When water was added (8.33; 16.67; 25; 33.33; 41.67; 50; 58.33; 66.67;75; and 83.33 percent) to the acetone solution of chlorophyll a, the chlorophylls aggregate, and in the presence ofbeta-carotene, the chlorophyll more stable. The water shifts the chlorophyll a spectrum toward red duringillumination with or without the presence of carotene. The formation of oligomeric chlorophyll a aggregate occurswithin 10-20 minutes after the water was applied. This strategy of aggregating the chlorophylls to dissipateexcess energy captured from light does not support the photostability of chlorophyll as much as beta-carotene.Surprisingly, when beta carotene was mixed with 66.7% and 75.0% of water, the chlorophyll a degraded 5.56% and9.71% respectively. In conclusion, the aggregate form of chlorophyll a and the presence of beta-carotene increasephotostability of chlorophyll a in acetone solution.

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