scholarly journals The Comparison Effect Of Energy Drinks And Coffee On Creatinin Level In Rats

2019 ◽  
Vol 3 (4) ◽  
pp. 231 ◽  
Author(s):  
Mohamad Riza ◽  
Andina Putri A
Keyword(s):  
Creatinine Level ◽  
Energy Drinks ◽  
Laboratory Research ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Significant Difference ◽  
Post Test ◽  
Experimental Laboratory ◽  
Post Hoc

Objective: to compare the effect of administration of energy drinks and coffee on creatinine levels in rats.Methods: an experimental laboratory research using the post test only control group design. The study used 18 male white Sprague Dawley divided into three groups to receive one of the following treatments: 2 cc distilled water,0.072 g /2cc energy drinks and 0.9 g/cc of coffee for 14 days. The creatinine level was evaluated. Data were analyzed by One Way ANOVA test.Results: The mean creatinine level in the aquades group, energy drinks, coffee group was 0.708 mg/dl, 2.05 mg/dl 1.861 mg/dl, respectively. Post Hoc test the LSD test showed a significant difference among these groups (p <0.05).Conclusion: There was a significant different effect between 0.072 g / 2cc energy drinks and 0.9 g/cc coffee on creatinine levels in rat.International Journal of Human and Health Sciences Vol. 03 No. 04 October’19 Page : 231-234

scholarly journals Pengaruh Kontrasepsi Suntik DMPA Terhadap Kenaikan Berat Badan Tikus Betina Galur Wistar

2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Dwi Retna Prihati
Keyword(s):  
Body Weight ◽  
Test Group ◽  
Laboratory Research ◽  
Control Group ◽  
Female Rat ◽  
Wistar Strain ◽  
Significant Difference ◽  
Post Test ◽  
Experimental Laboratory ◽  
Rat Strain

Abstract: DMPA Injectable Contraception, Body Weight. The purpose of this study to determine the effect of contraception DMPA injection to BB in female blood wistar strain female. The benefits of this study are expected to increase public knowledge about injectable contraception especially related to the side effects of weight gain. This type of research is an experimental laboratory. Research design Randomized pre-post test group with control, the sample size of 10 adult female mice Wistar strain divided into 2 groups. Data were analyzed by independent T test. Significant value of p <0.05. The result of this research is there is significant difference between control group and DMPA contraception treatment group (p = 0,008). In conclusion contraceptive DMPA affects body weight wistar female rat strain.

scholarly journals Gambaran Histopatologi Pankreas Tikus Putih (Rattus norvegicus) Pasca Perlakuan Iskemia-Reperfusi Ginjal

2019 ◽  
Vol 13 (1) ◽  
pp. 58
Author(s):  
Elva Rosiana ◽  
Wiwit Ade Fidiawati ◽  
Darmawi Darmawi
Keyword(s):  
Tissue Damage ◽  
Control Treatment ◽  
Laboratory Research ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
White Rats ◽  
Post Test ◽  
Experimental Laboratory ◽  
Histopathologic Feature

Ischemic-reperfusion injury is defined by a condition of hypoperfusion in the spesific organ followed by reperfusion(reoxygenation) inducing tissue damage. Tissue damage produces reactive oxygen species (ROS) leading to oxidativestress condition. Oxidative stress mediate the lipid peroxidation reactions, harm the cell and finally facilitate the celldeath. The aim of this study was to evaluate the histopathologic feature of white rat’s pancreas post renal ischemiareperfusion.This study was an experimental laboratory research with post-test-only control group design including 20male white rats. Rats were divided into 5 groups, control, treatment 1 (45 minutes ischemia), treatment 2 (45 minutesischemia followed by 1 hour reperfusion), treatment 3 (45 minutes ischemia followed by 2 hours reperfusion) andtreatment 4 (45 minutes ischemia followed by24 hours reperfusion). We found the change of the histopathologicalcharacteristics of white rat’s pancreas in term of edema, leukocyte infiltration and vacuolization.

scholarly journals JUS SEMANGKA MENURUNKAN NEUTROFIL TIKUS JANTAN GALUR WISTAR YANG TERPAPAR ASAP ROKOK

2018 ◽  
Vol 11 (2) ◽  
pp. 166
Author(s):  
Rizky Prihandari ◽  
Lailatul Muniroh
Keyword(s):  
Cigarette Smoke ◽  
Hazardous Substances ◽  
Male Rats ◽  
Laboratory Research ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Wistar Strain ◽  
Post Test ◽  
Experimental Laboratory

Cigarette smoke contains many hazardous substances and free radical which affect respiratory system. Watermelon “Sugar Baby” juice contain lycopene as antiinfl ammation. The aim of this research was to analyze the effect of watermelon as antiinfl ammation toward neutrophils count in wistar strain male rats exposed by  cigarette smoke. This study was an experimental laboratory research with pre and post test control group design. Sample consisted of 24 wistar strain male rats with ±150-230 grams weight and 3 months old which were divided into four groups. Control group was given placebo (aquadest) and treatment groups were given w atermelon juice with terraced quantity (1.5 ml, 2.5 ml, and 3.5 ml). Every group was given two sticks of cigarette smoked per day during two weeks then smoked with treatment during one week. Neutrophil from peripheral smear was observed by manual method and statistically analyzed using Anova Mixed Design and continued with One Way Anova. The result showed signifi cant increased of  neutrophil at second observation on control group (p=0.002) and treatment groups (p=0.000). Signifi cance decreased also found in the treatment groups’s at third observation p=0.000. Signifi cant between each group p= 0.017. There was signifi cant different between control and treatment groups but there was no signifi cant different between each treatment groups. This study concluded that there was decreased of  neutrophil in wistar strain male rats which were given w atermelon juice 1.5 ml, 2.5 ml, and 3.5 ml during infl ammation. 3.5 ml w atermelon juice which were given showed better result as antiinfl ammation..

scholarly journals PENGARUH PEMBERIAN BUAH PEPAYA (Carica papaya L.) TERHADAP KADAR KOLESTEROL LDL DAN KOLESTEROL HDL PADA TIKUS SPRAGUE DAWLEY DENGAN HIPERKOLESTEROLEMIA

2012 ◽  
Vol 1 (1) ◽  
pp. 258-264
Author(s):  
Widitha Gustining Asmariani ◽  
Enny Probosari
Keyword(s):  
Cholesterol Level ◽  
Ldl Cholesterol ◽  
Direct Method ◽  
Hdl Cholesterol ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Post Hoc

Background: Increased LDL cholesterol concentrations and low plasma HDL cholesterol concentrations are well-established risk factors for cardiovascular disease. Diet modification is one of recommended theraphy to decrease LDL cholesterol level by increasing fiber intake from fruit. Papaya contains niacin, fiber, and antioxidant which can decrease LDL cholesterol level. It also contains quercetin to increase HDL cholesterol level. The aim of this study was to prove the effect of papaya of different dosages on LDL and HDL cholesterol of hypercholesterolemic rats. Methods: This research was true-experimental using pre-post test with control group design. Subjects were male Sprague Dowley rats, 7-8 weeks old, 100-200 grams weight, inducted hypercholesterolemia, given papaya diet using 5,4 gr, 7,2 gr, and 9,0 gr dosage for 4 weeks. LDL cholesterol and HDL cholesterol were measured by direct method using LDL-C Select (DiaSys) reagent and CHOP-PAP methods respectively. Normality of the data was tested by Shapiro Wilks test. Data were analyzed by paired t test continued with One Way Anova.and post-hoc LSD. Result: The study revealed that papaya of dosage 5,4 gr/day decreased LDL cholesterol (p<0,05) significantly from 45,29±9,05 mg/dl to 30,29±5,31 mg/dl but nonsignificantly decrease HDL cholesterol level (p=0,930) from 25,71 ± 5,09 to 25,57 ± 3,59 mg/dl at the same dosage. Other dosage of 7,2 mg/day and 9,0 gr/day neither significantly decrease LDL cholesterol level nor increase HDL cholesterol level. Conclusion: Papaya couldn’t decreased LDL cholesterol and increased HDL cholesterol in hypercholesterolemic rats.

scholarly journals PENGARUH PEMBERIAN JUS BIJI PEPAYA (CARICA PAPAYA LINN.) TERHADAP KADAR ASAM URAT TIKUS SPRAGUE DAWLEY DISLIPIDEMIA

2013 ◽  
Vol 2 (1) ◽  
pp. 184-191
Author(s):  
Cut Arsyiyanti ◽  
Ahmad Syauqy ◽  
Kusmiyati Tjahjono
Keyword(s):  
Carica Papaya ◽  
T Test ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Randomized Control ◽  
Post Hoc ◽  
Paired T Test

Latar Belakang : Asam urat merupakan produk akhir metabolisme purin yang jika jumlahnya berlebih dapat memicu berbagai macam penyakit diantaranya gout. Biji pepaya mengandung zat fitokimia seperti flavonoid, saponin, dan tanin yang dapat menormalkan kadar profil lipid dan menurunkan kadar asam urat. Penelitian ini bertujuan untuk mengetahui pengaruh jus biji pepaya terhadap kadar asam urat pada tikus dislipidemia. Metode: Jenis penelitian ini adalah true experimental dengan pre-post test randomized control group design terhadap 24 ekor tikus Sprague Dawley dislipidemia yang kemudian dibagi secara acak dalam 4 kelompok yaitu kelompok kontrol negatif yang hanya diberikan pakan standar, kontrol positif yang diberikan pakan standar dan tinggi lemak, serta dua kelompok perlakuan yang diberikan pakan standar, tinggi lemak dan jus biji pepaya dengan dosis 400 mg dan 800 mg selama 30 hari. Kadar Asam urat diperiksa dengan metode Spektrofotometri. Data di analisis dengan uji Paired t-test dan Anova serta uji LSD pada tingkat kepercayaan 95%. Hasil: Perubahan kadar asam urat kelompok kontrol negatif, k ontrol positif dan perlakuan 400 mg dan 800 mg secara berturut-turut adalah -11,21 (p=0,352), 18,91 (p=0,360), -30,43 (p=0,024), dan -16,67(p=0,127). Perubahan kadar asam urat antar kelompok dengan uji Anova menunjukkan signifikansi sebesar 0,017. dilanjutkan uji Post-Hoc antara kelompok kontrol negatif dengan kelompok perlakuan dosis 400 mg dan 800 mg menunjukkan signifikansi berturut-turut 0,003 dan 0,019. Simpulan: Pemberian jus biji pepaya selama 30 hari pada dosis 400 mg/ekor/hari efektif menurunkan kadar asam urat pada tikus dislipidemia.

scholarly journals TINGKAT KEKERASAN PERMUKAAN RESIN KOMPOSIT AKIBAT MASA KEDALUWARSA MATERIAL

2018 ◽  
Vol 5 (1) ◽  
pp. 45
Author(s):  
Yuvika Intan Ristian Putri ◽  
Muhammad Dian Firdausy ◽  
Niluh Ringga Woroprobosari
Keyword(s):  
Surface Hardness ◽  
Composite Resins ◽  
Control Group ◽  
Expiration Date ◽  
Control Group Design ◽  
Expiry Date ◽  
Post Test ◽  
Experimental Laboratory ◽  
Post Hoc ◽  
One Way Anova

Background: Aplication of composites resin in relatively small amount as a restorative material often causing the material reaches its expiry date before all of the material can be used. Composites resin that have passed the expiry date would affect charateristic of the material. The aim of this study was to investigate surface hardness difference of composite resins based on material expiration date.Methods: This research was an experimental laboratory using post test only with control group design. Samples for this research were 27 divided into 3 groups based on its expiration date (2016, 2018 and 2019). The surface hardness was measured by Shore D Hardness. The result of this research were tested with One Way Anova and Post Hoc test LSD.Result: The lowest surface hardness result was measured on group 1 (81.02SHD) and the highest was group 3 (94.72 SHD). The result of One Way Anova test showed that there was signifcant differences in all groups (p<0.05). Post Hoc LSD test showed difference signifcantly between each groups.Conclusion: It can be concluded that there was signifcant difference of composite resins surface hardness based on material’s expiration date

scholarly journals The In Vitro Anti-Diabetic Activity of Lime Peels (Citrus amblycarpa (Hassk.) Ochse)

2020 ◽  
Vol 13 (01) ◽  
pp. 26-33
Author(s):  
Gempita Cahya aulia tambunan ◽  
Aparna Dutt ◽  
Sayra Nadhifa ◽  
Firdha Amelia ◽  
Ermi Girsang
Keyword(s):  
Control Group ◽  
Control Group Design ◽  
Phytochemical Content ◽  
Ic50 Value ◽  
Significant Difference ◽  
Post Test ◽  
Lime Peel ◽  
Post Hoc ◽  
One Way Anova

There are various potential natural anti-diabetic drugs; one of them is lime peel or Citrus amblycarpa. This study was aimed to explore the anti-diabetic activity and phytochemical content of lime peels. This study was an experimental study that used the post-test only control group design. The lime peels that were collected from the Berastagi fruit market in Medan, North Sumatera were extracted using 70% ethanol by maceration methods. The phytochemical screening identified the presence of phenolic, steroid/triterpenoid, terpenoid, saponin, flavonoid, tannin, and alkaloid. Meanwhile, the anti-diabetic activity of lime peels was evaluate using the α-glucosidase enzyme that was gotten from Saccharomyces cerevisiae by α-glucosidase enzyme inhibition methods. Percent of inhibition was express as Mean ± SD and analyzed by One Way ANOVA, Tukey HSD Post Hoc Test, and followed by linear regression. The result of this study showed that there is a significant difference in percentage inhibition α-glucosidase enzyme in each concentration, and it had an IC50 Value amount of 125.93 ± 9.14 µg/mL. The phytochemical content of the lime peels was flavonoid, phenol, steroid/triterpenoid, and alkaloid. Hence, the lime peel has anti-diabetic activity by inhibition of the α-glucosidase enzyme.

scholarly journals PERBEDAAN PENGARUH ANTARA EKSTRAK DAN REBUSAN DAUN SALAM (Eugenia polyantha) DALAM PENCEGAHAN PENINGKATAN KADAR KOLESTEROL TOTAL PADA TIKUS SPRAGUE DAWLEY

2014 ◽  
Vol 3 (1) ◽  
pp. 142-149
Author(s):  
Siti Muflikhatur R ◽  
Hesti Murwani Rahayuningsih
Keyword(s):  
T Test ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Randomized Control ◽  
Post Hoc ◽  
One Way Anova ◽  
Paired T Test

Latar Belakang: Peningkatan kadar kolesterol total merupakan faktor risiko terjadinya penyakit kardiovaskuler.  Manajemen kadar kolesterol total dapat dilakukan dengan upaya kuratif dan preventif. Simvastatin merupakan salah satu obat penurun kadar kolesterol total. Pengendalian asupan efektif untuk mencegah peningkatan kadar kolesterol total. Konsumsi makanan fungsional berpotensi dalam mencegah peningkatan kadar kolesterol total, salah satunya adalah konsumsi daun salam. Flavonoid yang terkandung dalam daun salam terbukti efektif dalam menurunkan kadar kolesterol total. Tujuan penelitian ini adalah untuk membuktikan adanya perbedaan pengaruh antara ekstrak dan rebusan daun salam dalam mencegah peningkatan kadar kolesterol total darah tikus Sprague dawley.Metoda: Rancangan penelitian adalah true experimental jenis pre-post test randomized control group design terhadap 24 ekor tikus Sprague dawley yang dibagi acak dalam 4 kelompok. Tikus diberi pakan tinggi lemak bersamaan dengan pemberian ekstrak dan rebusan dengan dosis masing-masing ekstrak dari 0,72 gram daun segar dan rebusan 0,72 gram secara sonde sekali sehari. Kadar kolesterol total diperiksa dengan metode CHOD-PAP spektrofotometri. Data dianalisis menggunakan paired t-test dan uji one way ANOVA yang dilanjutkan uji Post-Hoc LSD pada tingkat kepercayaan 95%.Hasil: Kadar kolesterol total pada seluruh kelompok meningkat secara signifikan (p=0,000). Peningkatan kadar kolesterol total pada kelompok kontrol negatif adalah 147,88 %, kelompok control positif 11,64 %, kelompok perlakuan ekstrak 39,03 %, dan kelompok perlakuan rebusan 77,84 %. Terdapat perbedaan perubahan kadar kolesterol total antar kelompok secara signifikan (p=0,000).Simpulan: Perlakuan yang memiliki efek menahan laju peningkatan kadar kolesterol total terbesar sampai terkecil adalah 0,018 gram simvastatin, 0,034 gram ekstrak daun salam, dan 0,72 gram rebusan daun salam.

scholarly journals Manfaat pemberian tepung daun kelor (Moringa oleifera) terhadap kadar IL-6 dan kolesterol LDL tikus Sprague Dawley dislipidemia

2021 ◽  
Vol 6 (2) ◽  
pp. 173
Author(s):  
Welresna Juliatri Putri Rupiasa ◽  
Siti Fatimah-Muis ◽  
Ahmad Syauqy ◽  
Kusmiyati Tjahjono ◽  
Gemala Anjani
Keyword(s):  
Oxidative Stress ◽  
Intervention Group ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Sd Rats ◽  
High Fat Diets ◽  
Post Test ◽  
Fat Diets ◽  
Post Hoc

Dyslipidemia condition causes oxidative stress that triggers inflammation. Moringa leaves are rich in fiber and antioxidants that can prevent oxidative stress and reduce inflammation. This study aimed to see, measure, and analyze the effect of Moringa flour supplementation on IL-6 and LDL levels of dyslipidemic Sprague Dawley (SD) rats. The type of study was true-experiment with randomized pre-post. Test with control. Group design. Intervention for 28 days in 4 groups of SD rats (n= 24); a consisting group of healthy rats (K-), dyslipidemic rats (K+), dyslipidemic rats respectively given 0.1g/100g BW moringa flour (P1), and 0.2 g/100g BW moringa flour (P2). ELISA and CHOD-PAP methods measured IL-6 and LDL levels. Statistical analysis used Paired t-test, One-way Anova, and Post Hoc Bonferroni. The results showed a significant decrease in IL-6 and LDL levels in the intervention group compared to the non-intervention group (p= 0,000). The reduction of IL-6 levels in the P2 group was greater than that in the P1 group but not significant. The decrease. in LDL levels in the P2 group was significantly higher than in the P1 group. It is attributed to the fiber and antioxidant content in Moringa. Fiber that can bind cholesterol and bile acids, antioxidants can regulate fat metabolism, capture free radicals and prevent oxidative stress. In conclusion, Moringa flour supplementation improved lipid profiles and inflammation compared to rats with only standard and high-fat diets

scholarly journals PERBEDAAN PERUBAHAN KADAR KOLESTEROL LDL SETELAH PEMBERIAN EKSTRAK DAN REBUSAN DAUN SALAM (EUGENIA POLYANTHA) PADA TIKUS SPRAGUE DAWLEY YANG DIBERI PAKAN TINGGI LEMAK

2014 ◽  
Vol 3 (1) ◽  
pp. 90-97
Author(s):  
Astri Praba Shinta ◽  
Martha Irene Kartasurya
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Randomized Control ◽  
Post Hoc ◽  
Paired T Test

LatarBelakang : Dislipidemia merupakan gangguan metabolisme lipid berupa peningkatan kadar kolesterol total, kolesterol Low Density Lipoprotein (LDL), trigliserida, dan penurunan kolesterol High Density Lipoprotein (HDL). Quercetin yang terkandung dalam flavonoid daun salam dapat menurunkan kadar kolesterol LDL.Metode : Jenis penelitian ini adalah eksperimental laboratorik dengan pre-post test randomized control group design. Subjek penelitian adalah 24 tikus Sprague Dawley jantan berumur 8 minggu yang dibagi menjadi 4 kelompok, yang terdiri dari 6 ekor tikus. Keempat kelompok diberi pakan standar dan diet tinggi lemak. Kelompok pertama merupakan kelompok kontrol positif dan kelompok kedua diberi 0,18 g/kg BB simvastatin. Kelompok ketiga diberi 0,034 g/kg BB ekstrak daun salam dan kelompok keempat diberi 0,72 g/kg BB rebusan daun salam. Kadar kolesterol LDL ditentukan dengan metode CHOD-PAP. Normalitas distribusi data diuji dengan Shapiro-Wilks. Data dianalisis dengan uji paired t-test, Kruskall Wallis, dan One Way Anova.Hasil : Hasil penelitian menunjukkan adanya peningkatan kadar kolesterol LDL yaitu 79,7 mg/dL pada kelompok kontrol positif; 38,3 mg/dL pada kelompok rebusan; 26,2 mg/dL pada kelompok ekstrak; serta 2,9 mg/dL pada kelompok simvastatin. Kelompok kontrol positif mengalami peningkatan kadar kolesterol LDL paling tinggi dibandingkan dengan kelompok lainnya. Peningkatan kadar kolesterol LDL pada kelompok ekstrak (26,2 mg/dL) lebih rendah dibandingkan dengan kelompok rebusan (38,3 mg/dL). Hasil uji Post Hoc menunjukkan bahwa terdapat perbedaan perubahan kadar kolesterol LDL antara kelompok simvastatin dan kontrol positif; simvastatin dan ekstrak; simvastatin dan rebusan; ekstrak dan kontrol positif; ekstrak dan rebusan; serta rebusan dan kontrol positif. Simpulan : Pemberian rebusan daun salam tidak dapat menurunkan kadar kolesterol LDL pada tikus Sprague Dawley yang diberi pakan tinggi lemak namun peningkatan kadar kolesterol LDL pada kelompok rebusan lebih rendah dibandingkan dengan kelompok kontrol positif serta lebih tinggi peningkatannya dibandingkan dengan kelompok ekstrak dan simvastatin.

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