Ameliorating Effects of Fenugreek Seeds Powder against Hematotoxicity Induced by Aluminum Chloride in Male Rabbits

Background: Humans are exposed to aluminum from the mouth, nose and epidermal route inducing toxic effects. Accumulation of aluminum has been associated with a variety of pathologies such as anemia, osteodystrophy, joint diseases, muscular weakness, and Alzheimer’s diseases Fenugreek extracts have been shown to be neutralizing of free radicals and enhancing antioxidant status. Objectives: The present study aimed to evaluate the ameliorative effects of fenugreek seeds against hematotoxicity induced by Aluminum chloride in male rabbits. Materials and Methods: This study included twenty-four adult male rabbits, which were divided into 4 groups, 6 rabbits for each. Group I (control group): Animals were provided with tap water and fed with a normal diet for 30 days. Group II (Fenugreek seeds powder group): Fenugreek seeds powder was given to rabbits in food at a dose of 10 g per kilogram of diet weight/kg of body weight/day for 30 Days. Group III (Aluminum chloride (ALCl3) group): Rabbits were treated orally with 150 mg/kg BW of AlCl3/day for 30 consecutive days. Group IV (Aluminum chloride/fenugreek co-administered group): Fenugreek seeds flour was added at a rate of 10 g per kilogram of diet weight, and rabbits were treated orally with 150 mg/kg BW of AlCl3/day for 30 consecutive days. At the end of the experiment and 24 hours after the last dose, all animals were anesthetized with ether and blood samples were collected by heart puncture. Results: The results of the study showed that the treatment of male rabbits with aluminum chloride resulted in a significant decrease (P<0.01) in RBCs count, hemoglobin concentration, hematocrit value, MCV, MCH, and MCHC as compared to the control group. While there was a significant increase (P<0.01) in WBCs count, lymphocytes, and monocytes percentages and a significant decrease in granulocyte percentage when compared with the control group. Co-administration of fenugreek seeds powder and AlCl3 significantly improved all haematological parameters. Conclusion: The results showed that the administration of rabbits with aluminum chloride caused a hematotoxicity, and co-administration of fenugreek seeds powder with AlCl3 alleviate the hematotoxicity induced by AlCl3. The use of fenugreek seeds powder by humans can be considered beneficial in the alleviation of hematotoxicity. It is recommended that humans exposed to AlCl3 should be advised to take fenugreek seeds powder as a rich source of antioxidants to prevent hematotoxicity induced by AlCl3. Further studies are necessary to elucidate the exact mechanism of the anti-hematotoxic effect of Fenugreek seeds powder and the potential usefulness of fenugreek seeds powder as a protective agent against AlCl3 induced hematotoxicity in clinical trials.

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Ameliorating Effects of Fenugreek Seeds Powder against Hematotoxicity Induced by Aluminum Chloride in Male Rabbits

Biotechnology and Bioprocessing ◽

10.31579/2766-2314/035 ◽

2021 ◽

Vol 2 (4) ◽

pp. 01-09

Author(s):

Azab Elsayed Azab ◽

Munira Ammar Algridi

Keyword(s):

Aluminum Chloride ◽

Tap Water ◽

Hemoglobin Concentration ◽

Normal Diet ◽

Group Iv ◽

Control Group ◽

Protective Agent ◽

Fenugreek Seeds ◽

Group Iii ◽

Group I

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Ameliorative Effects of Fenugreek Seeds and Curcumin on Hematotoxicity induced by Nicotine in Male Albino Rats

Biotechnology and Bioprocessing ◽

10.31579/2766-2314/062 ◽

2022 ◽

Vol 3 (1) ◽

pp. 01-08

Author(s):

Azab Elsayed Azab ◽

Mohamed Omar Albasha ◽

Manal Abuelkasem Elnaif

Keyword(s):

Hematological Parameters ◽

Hemoglobin Concentration ◽

Treated Group ◽

Group Iv ◽

Control Group ◽

Albino Rats ◽

Group V ◽

Fenugreek Seeds ◽

Group Iii ◽

Group I

The present study aimed to investigate the ameliorative effects of fenugreek seeds and curcumin on hematotoxicity induced by nicotine in male albino rats. 30 male F-344/NHsd Fischer rats, weighing from 180 to 200g were used in the present study. The animals were divided into five groups (6 rats for each); Group I (control group), Group II (nicotine treated group), Group III (nicotine/fenugreek seeds co-administered), Group IV (nicotine/curcumin co-administered), and Group V (nicotine/curcumin& fenugreek seeds co-administered). At the end of the experimentation and 24 hours after the last dose, all animals were anaesthetized with ether and blood samples were collected by heart puncture. The samples were collected in clean dry tubes containing the anticoagulant substance EDTA and used for the hematological studies. The results showed that the animals treated with nicotine for 4 weeks showed a significant decrease in RBCs count, hemoglobin concentration, hematocrit value, MCH, MCHC, and platelets count, and increased MCV and WBCs count as compared to the control group. Co-administration of nicotine with fenugreek and/or curcumin caused improvement in all hematological parameters when compared with nicotine group. It can be concluded that nicotine had a strong effect on the hematological parameters. The ingestion of fenugreek and/or curcumin prevent the hematoxicity induced by nicotine. The current study suggests that fenugreek and curcumin may be useful in combating free radical-induced hematotoxicity induced by nicotine.

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A Study on the Effect of Samoa Extract (Cleome droserifolia) on Sperm Quality in Male Albino Rats Exposed to Pyrethroid

Libyan Journal of Basic Sciences ◽

10.36811/ljbs.2021.110064 ◽

2021 ◽

pp. 39-45

Author(s):

Nura I. Al-Zail ◽

Salah F. Kamies

Keyword(s):

Sperm Quality ◽

Group Iv ◽

Male Rats ◽

Control Group ◽

Albino Rats ◽

Protective Agent ◽

Group V ◽

Group Iii ◽

Group I ◽

Induced Changes

Pyrethroid cyhalothrin (PC) is an insecticide that is used worldwide for pest control in agriculture and household use. Samoa extract (SE) is a potent antioxidant protecting cells from oxidative stress. The present study investigates the protective and therapeutic effect of SE on PC-induced changes in sperm quality in male rats. Fifty adult male albino rats were divided into five groups: group I: served as control; group II: received PC i.p. only (6.2 mg/kg b.wt.); group III: received SE only (100 mg/kg b.wt., p.o.) for eight weeks; group IV: received SE as a protective agent daily for eight weeks, then followed by the administration of PC (i.p.) three times a week for two weeks; group V: exposed to PC (i.p.) three times a week for two weeks, then treated with the SE daily for 8 weeks. Results showed that PC caused markedly impaired sperm quality (a count, viability, motility, and abnormality). Compared to PC-treated animals, SE in the protective group markedly restored the alteration of sperm indices. However, SE in the curative group was found to be less effective in restoring PC-induced alterations. In conclusion, the data of this study revealed that the SE as a protective agent is more effective than as a therapeutic agent. Keywords: Samoa; Pyrethroid; Sperm quality; Rat

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Antidyslipidemic Effect of Fenugreek Seeds Powder against Sodium Fluoride-Induced Dyslipidemia in Male Rabbits

Biotechnology and Bioprocessing ◽

10.31579/2766-2314/028 ◽

2021 ◽

Vol 2 (3) ◽

pp. 01-08

Author(s):

Azab Elsayed Azab ◽

Munira Ammar Algridi

Keyword(s):

Body Weight ◽

Sodium Fluoride ◽

Normal Diet ◽

Control Group ◽

Population Exposure ◽

Protective Agent ◽

Serum Concentrations ◽

Serum Triglycerides ◽

Fenugreek Seeds ◽

Group I

Background: Although fluoride is everywhere in the environment, the major environmental sources of population exposure to elevated levels of fluoride are water, food, beverages, air, food supplements, and dental products. Fluoride is a well determined non-biodegradable and moderate pollutant, which at high levels causes serious health problems. Objectives: The present study aimed to evaluate the anti-dyslipidemia effect of fenugreek seeds powder against dyslipidemia induced by sodium fluoride in male rabbits. Materials and Methods: This study included twenty-four adult male rabbits, which were divided into 4 groups, 6 rabbits for each. Group I (control group): Animals were provided with tape water and fed with normal diet for 30 days. Group II (Fenugreek seeds powder group): Fenugreek seeds powder was given to rabbits in food at a dose of 10 g per kilogram of diet weight/kg of body weight/day for 30 Days. Group III (sodium fluoride group): Rabbits were injected intraperitoneally with sodium fluoride at a dose of 15mg/kg of body weight/day for 30 consecutive days. Group IV (Sodium fluoride/fenugreek co-administered group): Fenugreek seed flour was added at a rate of 10 g per kilogram of diet weight, and rabbits were injected with intraperitoneally with sodium fluoride at a dose of 15mg/kg of body weight/day for 30 consecutive days. After thirty days, blood samples were taken for biochemical analysis. Biochemical analyzes were performed to measure of the serum concentrations of cholesterol, triglycerides, HDL-C, LDL-C, and VLDL-C in all groups. Results: The results of the study showed that the treatment of male rabbits with sodium fluoride resulted in a significant increase (P<0.01) in the serum concentrations of cholesterol, triglycerides, LDL-C, and VLDL-C, and a significant decrease was observed (P<0.01) in serum HDL-C compared with the control group. In rabbits received fenugreek seeds powder only, the serum triglycerides and VLDL-C were significantly (P<0.01) decrease and the serum HDL-C was significantly (P<0.05) increase compared with the control group. A significant decrease (P<0.01) was observed in serum cholesterol, triglycerides, LDL-C, and VLDL-C, and a significant increase was observed (P<0.01) in serum HDL-C in rabbits treated with sodium fluoride and fenugreek seeds powder compared with the sodium fluoride group, but these measurements did not reach normal levels in the control group. Conclusion: The results showed that injection of rabbits with sodium fluoride led to serum dyslipidemia, and the injection with sodium fluoride and treatment of fenugreek seeds powder alleviate dyslipidemia induced by sodium fluoride. The use of fenugreek seeds powder by humans can be considered beneficial in the alleviation of dyslipidemia. It is recommended that humans exposed to sodium fluoride should be advised to take Fenugreek seeds powder as a rich source of antioxidant to prevent serum dyslipidemia induced by sodium fluoride. Further studies are necessary to elucidate exact mechanism of the antidyslipidemic effect of Fenugreek seeds powder and potential usefulness of Fenugreek seeds powder as a protective agent against sodium fluoride induced dyslipidemia in clinical trials.

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Analysis of surface conditioning methods on core-veneer bond strength of CAD/CAM zirconia restorations

Technology and Health Care ◽

10.3233/thc-202539 ◽

2020 ◽

pp. 1-11

Author(s):

Anip K. Roy ◽

Govind N. Prasad ◽

Tushar V. Bhagat ◽

Saurabh Chaturvedi ◽

Vishwanath Gurumurthy ◽

...

Keyword(s):

Electron Microscope ◽

Bond Strength ◽

Shear Bond Strength ◽

Group Iv ◽

Control Group ◽

Group Iii ◽

Surface Conditioning ◽

Group I ◽

Alumina Particles ◽

Scanning Electron

BACKGROUND: The increased strength of zirconia has resulted in its widespread application in clinical dentistry. Nevertheless, the fracture of veneering porcelains remains one of the key reasons of failure. OBJECTIVE: The objective of this study was to compare and analyze the influence of surface conditioning methods on the core-veneer bond strength of zirconia restorations. METHODS: Thirty specimens of zirconia core with sizes 10 × 5 × 5 mm were layered with porcelain of sizes 5 × 3 × 3 mm. On the basis of different surface conditioning methods, four groups were made: Group I: abrasion with airborne alumina particles of 110 μm size, Group II: sandblasting with silica coated alumina particles of 50 μm in size, Group III (modified group): alteration with a coating of zirconia powder prior to sintering, and Group IV (control group): metal core specimens. The shear force of all specimens was tested using a universal testing machine with a 0.5 mm/min crosshead speed. One-way analysis of variance (ANOVA) and Tukey’s post hoc pair wise comparison (p= 0.05) were performed to analyze the shear bond strength. A scanning electron microscope was used to assess the fractured specimens. RESULTS: A statistically significant difference was noted between the groups. The mean value of shear bond strength was 40.25 MPa for Group I, 41.93 MPa for Group II, 48.08 MPa for Group III and 47.01 MPa for Group IV. CONCLUSIONS: The modified zirconia group and control group demonstrated a significantly higher mean bond strength than that of Group I, where airborne particle abrasion was used. The scanning electron microscope showed that cohesive fracture in the porcelain veneers was the main problem of failure in altered zirconia. The modified zirconia specimens in Group III demonstrated significantly improved values of shear bond strength.

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Adaptive dose response pilot study of intravaginal Prostaglandin E2 application in free farrowing sows during parturition

10.21203/rs.3.rs-22844/v1 ◽

2020 ◽

Author(s):

Ramona Bill ◽

Heiko Nathues ◽

Alexander Grahofer

Keyword(s):

Prostaglandin E2 ◽

Total Duration ◽

Group Iv ◽

Control Group ◽

Birth Process ◽

Group Iii ◽

Beneficial Effects ◽

Group I ◽

Group Ii ◽

Meconium Staining

Abstract Background The duration of birth is an important factor influencing the survival of piglets and the health of sows. A prolonged parturition is usually treated with oxytocin, even though several undesirable side effects are described. Therefore, the aim of this study was to evaluate the safety and efficacy of Prostaglandin E2 (PGE2) of different concentrations as an intravaginal applied gel in sows. MethodsTwelve sows were randomly allocated to one of four treatment groups: Group I (control group) application of placebo gel; Group II application of 2.0 mg PGE2; Group III application of 1.0 mg PGE2; Group IV application of 0.5 mg PGE2. The gel was administered intravaginal after the birth of the fourth piglet. Total duration of birth (time between first piglet and last placenta), piglet interval and placenta expulsion duration (time between first and last placenta) were recorded, and each piglet was scored for meconium staining and vitality. Furthermore, stillborn piglets were categorized into ante-partum and intra-partum deaths. Results Although no significant differences between the groups were detected, a beneficial tendency in several parameters was observed in group III. The duration of birth was 284 min (average of 14.3 piglets per litter), whereas in all other groups it was more than 400 min (average of 18.0 piglets per litter). The piglet interval was 10.1 min in group II compared to 10.2 min in group III, 16 min in group IV and 21.4 min in group I. In group III the placenta expulsion duration was 119 min in contrast to the other groups with more than 266 min. Severe meconium staining in more than 10% of piglets was observed in group II and IV. Moreover, piglets of group II showed oedematous and haemorrhagic umbilical cords, lethargy and anoxia, and intra-partum deaths were recorded. Conclusion Although the sample size is limited in this study, it is assumed that an application of 1mg PGE2 intra-partum instead of lower or higher dosages has the most beneficial effects on the birth process in sows and on the vitality of piglets. Further investigation is necessary to confirm the positive effect of PGE2 in daily practise.

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ESTUDO COMPARATIVO ENTRE O FIO DE NAILON E A LINHA DE PESCA (POLIAMIDA) E A SUA APLICABILIDADE COMO FIO CIRÚRGICO

Archives of Veterinary Science ◽

10.5380/avs.v4i1.3788 ◽

1999 ◽

Vol 4 (1) ◽

Author(s):

SHEILA CANEVESE RAHAL ◽

MANOEL ÁLVARO GUIMARÃES ◽

JOSÉ VICENTE FORTES ◽

HÉLIO LANGONI ◽

PEDRO HELIO LUCCHIARI

Keyword(s):

Adverse Effect ◽

Comparative Study ◽

Physical Properties ◽

Group Iv ◽

Experimental Results ◽

Control Group ◽

Group Iii ◽

Nylon Thread ◽

Group I ◽

Mucous Membranes

"Linhas de pesca"* em diâmetros de 0,15mm; 0,20mm; 0,25mm; 0,30mm foram divididas em quatro grupos, contendo dez amostras de cada diâmetro: grupo I - controle; grupo II - linhas colocadas em frasco com pastilhas de formalina; grupo III - um ciclo de autoclavagem (30 minutos à 121 C, seguido por 10 minutos de secagem à 37 C em estufa); grupo IV - dois ciclos de autoclavagens. Foram avaliadas quanto: comprimento, calibre, resistência no nó e alongamento. Comparativamente foram examinados fios de náilon cirúrgico nas numerações 0, 1-0, 2-0, 3-0, 4-0 e 5-0 (10 amostras de cada um). Pelos resultados da análise estastística, verificou-se que a esterilização pelo calor úmido exerceu um efeito adverso na resistência da "linha de pesca". Por outro lado não houve diferenças na resistência, calibre e alongamento da "linha de pesca" entre o primeiro e segundo ciclo de autoclavagem. As linhas mantidas em pastilhas de formalina mantiveram na sua maioria suas características originais de resistência, comprimento, diâmetro e alongamento quando comparada ao grupo controle, entretanto, cabe lembrar que o formaldeído é considerado irritante para pele e membrana mucosa. Foi possível concluir que a "linha de pesca" apresenta características semelhantes ao fio cirúrgico, podendo ser mantida em pastilhas de formalina ou esterilizada em autoclave, lembrando que a autoclavagem altera algumas de suas propriedades físicas. Abstract A comparative study has been carried out between fishing lines and nylon thread in regard to their applicability as surgical thread. Four groups of 10 fishing lines in each of the following diameters = 0.15, 0.20, 0.25 and 0.30 mm = were subjected to the following treatments: group I, control; group II sterilized with formalin tablets; group III, autoclaved 30 minutes at 121oC; group IV, autoclaved twice 30 minutes at 121oC. The following parameters were then considered: length, caliber, resistance at the knot, and elongation. Ten samples of each surgical 0, 1.0, 2.0, 3.0, 4.0 and 5.0 nylon threads were used in comparatively similar experiments as the ones carried out with the fishing lines. According to the statistical analyses of the experimental results, it has been found that the sterilization by means of the humid heat exerts an adverse effect on the resistance of the fishing line. However, no differences were found between the effect displayed by two cycles sterilization compared with the fishing lines subjected to only one cycle. The threads maintained in formalin kept the majority of their original properties of resistance, length, diameter and elongation when compared with the control group. It is known, however that formaldehyde is an irritant chemical towards the skin and the mucous membranes. It was possible to conclude: a. fishing lines display characteristics similar to that ones of the nylon surgical thread; b. they can be maintained in formalin solution; c. autoclave sterilization alters some of their physical properties.

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Protective effect of L-carnitine against oxidative damage caused by experimental chronic aflatoxicosis in quail (Coturnix coturnix)

Acta Veterinaria Hungarica ◽

10.1556/avet.53.2005.3.5 ◽

2005 ◽

Vol 53 (3) ◽

pp. 319-324 ◽

Cited By ~ 7

Author(s):

M. Citil ◽

V. Gunes ◽

O. Atakisi ◽

Ayla Ozcan ◽

M. Tuzcu ◽

...

Keyword(s):

Drinking Water ◽

Whole Blood ◽

Reduced Glutathione ◽

Group Iv ◽

Control Group ◽

Total Aflatoxin ◽

Group Iii ◽

Coturnix Coturnix ◽

Group I ◽

Plasma Malondialdehyde

This study was designed to evaluate the effect of L-carnitine supplementation on the plasma malondialdehyde (MDA) and whole blood reduced glutathione (GSH) concentrations in experimentally-induced chronic aflatoxicosis in quails. For this purpose, a total of 80 quails up to 8 weeks old were divided into four equal groups. Group I served as control, Group II was given L-carnitine at the dose of 200 mg/litre in the drinking water for 60 days, Group III was given 60 µg total aflatoxin/kg diet for 60 days, and Group IV was given both 60 µg total aflatoxin/kg diet and 200 mg L-carnitine/litre in the drinking water for 60 days. Aflatoxin treatment caused a significant increase in plasma MDA and a significant decrease in blood GSH concentrations. On the other hand, there was a significant decrease in plasma MDA and a significant increase in whole blood GSH in the L-carnitine-supplemented group. The present study demonstrated that L-carnitine brought about the inhibition of lipid peroxidation by enhancing antioxidant capacity in quails with chronic aflatoxicosis.

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Comparative Evaluation of Apical Sealing Ability of Different Obturation Techniques by Confocal Microscopy: An In Vitro Study

Dental Journal of Advance Studies ◽

10.1055/s-0040-1713579 ◽

2020 ◽

Vol 8 (02) ◽

pp. 55-59

Author(s):

Reetu Arora ◽

Yogesh Kumar ◽

Neetu Jindal ◽

Renu Aggarwal ◽

Kavneet Takhar

Keyword(s):

Root Canal ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Group Iv ◽

Control Group ◽

Group Vi ◽

Group V ◽

Group Iii ◽

Group I

Abstract Introduction The aim of obturation in the root canal is to completely seal the canal space to eliminate all the portals of entry and exit between root canal and periodontal space. Various techniques have been developed to achieve a hermetic seal. Materials and Methods As many as 150 extracted human maxillary central incisors were taken for the study. Biomechanical preparation was done up to F5 protaper file. According to different obturation techniques, samples were divided into six groups, keeping 30 samples in experimental and 15 samples in control groups. Group I–Lateral Condensation, Group II–Thermafil, Group III–Beefill, Group IV–GuttaFlow, Group V–Positive Control group, Group VI–Negative Control group. After obturation, the samples were immersed in 2% Rhodamine-B dye for 24 hours. Each sample was longitudinally sectioned to examine under confocal laser scanning microscope. Statistical Analysis The results were evaluated with ANOVA and posthoc Tukey honest significant difference (HSD) comparison test. Results The mean values of dye penetration of different groups were Group I (Lateral Condensation) 1.51 ± 0.451, Group II (Thermafil) 0.918 ± 0.399, Group III (Beefill) 1.30 ± 0.559. Group IV (GuttaFlow) 0.655 ± 0.396, Group V (Positive Control group) 1.96 ±0.046, Group VI (Negative Control group) 0 ± 0. The lowest mean value of apical microleakage was found in GuttaFlow amongst all experimental groups. Conclusion It can be concluded that the GuttaFlow obturating material exhibited better apical sealing ability with canal walls.

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Remifentanil and Propofol Sedation for Retrobulbar Nerve Block

Anaesthesia and Intensive Care ◽

10.1177/0310057x0203000405 ◽

2002 ◽

Vol 30 (4) ◽

pp. 433-437 ◽

Cited By ~ 17

Author(s):

V. Rewari ◽

R. Madan ◽

H. L. Kaul ◽

L. Kumar

Keyword(s):

Adverse Effects ◽

Respiratory Depression ◽

Study Groups ◽

Group Iv ◽

Control Group ◽

Group Iii ◽

Group I ◽

Retrobulbar Block ◽

Significant Movement ◽

Group Ii

We studied remifentanil and propofol for analgesia and sedation during the placement of an ophthalmic block. Eighty ASA I or II patients undergoing elective cataract surgery under a retrobulbar block in a rural camp setting were included in the study. Patients were randomly divided into four groups and received different drug combinations as follows: Group I—remifentanil 1 μg/kg, Group II—remifentanil 0.5 μg/kg and propofol 0.5 mg/kg, Group III— remifentanil 1 μg/kg with propofol 0.5 mg/kg and Group IV—saline 0.1 ml/kg. Patients were observed for degree of movement, sedation, pain, recall and respiratory depression. No patient in the study groups reported pain or displayed movement whereas most of the patients in the control group had significant pain during the placement of the block. Also, seven (35%) patients in the control group showed significant movement which may have led to failure of block in two patients and retrobulbar haemorrhage in one patient. Incidence of significant respiratory depression was maximum in Group III patients (60%), followed by Group I (20%) and least in Group II (5%). All patients in the study groups remained cooperative and obeyed commands except four patients in group III (OAA/S-4). Postoperatively, other than the control group, recall was maximum in Group I (55%) and least in Group II (5%). Hence, a combination of remifentanil 0.5 μg/kg with propofol 0.5 mg/kg as a bolus was considered to provide excellent relief of pain and anxiety with least adverse effects for the placement of ophthalmic blocks.

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