ІМУНОГІСТОХІМІЧНІ КРІТЕРІАЛЬНО-ВАЖЛИВІ ПОКАЗНИКИ ЛІМФАНГІОГЕНЕЗУ РАКУ ЯЄЧНИКІВ

An in-depth study of the biology of tumor growth will help to identify factors that allow us to understand the pathogenetic mechanisms of the development of ovarian cancer metastasis and progression, as well as to become a theoretical basis for developing new approaches to the treatment of this disease. The aim of this study was to determine immunohistochemical and endothelial criteria for ovarian cancer. The postoperative samples of ovarian tumor tissues were divided into 3 groups: comparison group - ovarian cancer; main group - borderline ovarian tumor; benign ovarian tumors. The study was conducted according to the FIGO 2009 classification. The International Histological Classification of WHO 2013 Female Genital Tumors was used for morphological characteristics. Level of growth factors - sVEGF-A was performed by ELISA using standard test systems (BenderMedSystem, Austria). IGC material studies were performed on serial paraffin sections using a standard method with murine monoclonal antibodies to p53 (clone D0-7. Dilution 1: 100. "Dako"). Ventana Medical Systems, Inc. was used as the detection system. Positive and negative control reactions were performed. The label index (MI) was used to evaluate p53 nuclear expression. WCIF ImageJ and Aperio Image Scope were used to estimate the number and degree of cell staining. Statistical analysis of the obtained data was performed using Statistica 6.0. The results of morphological studies of ovarian cancer showed that in our study patients with serous cancer predominated - 78.5%. The second most frequently diagnosed cancer was undifferentiated. In the second stage of our study, we conducted a comparative analysis of the concentration of p53 in the serum and tissue of patients in the study groups, which showed the existence of significant differences. In patients of the POY and DOY groups, both total and local p53 protein activity were significantly higher than in the comparison group, p <0.05. There was a positive correlation between p53 protein activity in serum and ovarian tissue. Serum VEGF A scores were statistically significantly correlated with the disease stage: Spearman rank correlation coefficient rho = 0.30; 95% CI = 0.02 - 0.536, p <0.05. There were no correlations with patients' age, histological subtype, and degree of tumor differentiation. Considering the results of our study, we can conclude that the criterion-important indicators of QA are serum levels of p53 and the index of serum VEGF A, which is confirmed by the results of ROC analysis p = 0.0026, and indicates a good informativeness of the method.

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RETROSPECTIVE STUDY AND CURRENT REALITIES AND POSSIBILITIES OF SURGICAL TREATMENT OF OVARIAN CANCER

Kharkiv Surgical School ◽

10.37699/2308-7005.2.2020.38 ◽

2020 ◽

pp. 201-207

Author(s):

S. M. Kartashov ◽

T. V. Bazarіnska ◽

M. Ye. Tymchenko ◽

S. M. Gramatyuk

Keyword(s):

Ovarian Cancer ◽

Surgical Treatment ◽

Ovarian Tumor ◽

Comparison Group ◽

Main Group ◽

Greater Omentum ◽

Benign Tumors ◽

Borderline Ovarian Tumor ◽

Childbearing Age ◽

Increased Risk

Summary. The aim of the study was to study the effect of clinical and morphological factors on diagnosis and onset of disease recurrence. Materials and methods. To achieve this goal, we formed a sample of postoperative samples of ovarian tumor tissue, which were divided into 3 groups: comparison group — ovarian cancer (T1-3N0M0, T1-3N0M1) (РЯ n = 261) main group 1 — borderline ovarian tumor (T1-3N0M0) (singing n = 100); the main group 2 — benign tumors of the ovaries (DOYA n = 40). The age of patients ranged from 23 to 62 years. Of these, 50 % were women of childbearing age — from 23 to 36 years (n = 26). The comparison group included 46 patients diagnosed with ovarian cancer aged 35 to 78 years. Among them, the age group from 30 to 40 years old was 14 % (n = 6). Most of the observations — 56 % (n = 24) — occurred in patients aged 40-60 years. Women over 60 made up 30 % (n = 14). Results and discussion. Ovarian cancer in the structure of the female genital organs malignancy is characterized by an ambiguous forecast and the highest mortality rate. The leading factors determining this phenomenon are the features of tumor metastasis. Metastasis of ovarian cancer occurs at the early stages of the disease and runs a variety of ways: contact, intraperitoneal, haemacirculatory and through the lymphatic system. Such features are determined by the topography of metastatic disease, anatomy, blood supply and lymphatic channel of the female reproductive gland. The greater omentum plays the barrier role in the development of the pathological process of the abdomen. At present poor prognosis and poor treatment outcomes require reviewing approaches to the surgical treatment of ovarian cancer. In this connection defining the features of ovarian cancer metastasis provides an opportunity to find new ways to disable malignant cells distribution. Conclusions. A key role in the treatment of ovarian cancer has surgery, chemotherapy, and targeted therapy. Adequate staging is important in choosing treatment tactics and assessing the prevalence of the disease. The importance of genetic and molecular studies is growing, according to the results of which it is possible to predict an increased risk of developing OC, to individualize and adjust treatment regimens.

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Mucinous borderline ovarian tumor versus invasive well-differentiated mucinous ovarian cancer: Difference in characteristics and outcomes

Gynecologic Oncology ◽

10.1016/j.ygyno.2019.02.003 ◽

2019 ◽

Vol 153 (2) ◽

pp. 230-237 ◽

Cited By ~ 6

Author(s):

Koji Matsuo ◽

Hiroko Machida ◽

Rachel S. Mandelbaum ◽

Brendan H. Grubbs ◽

Lynda D. Roman ◽

...

Keyword(s):

Ovarian Cancer ◽

Ovarian Tumor ◽

Borderline Ovarian Tumor ◽

Well Differentiated

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Diagnosis shift between low-grade serous ovarian cancer and serous borderline ovarian tumor: A population-based study

Gynecologic Oncology ◽

10.1016/j.ygyno.2020.05.085 ◽

2020 ◽

Vol 159 ◽

pp. 95-96

Author(s):

S. Matsuzaki ◽

H. Machida ◽

S. Matsuzaki ◽

M. Klar ◽

B.H. Grubbs ◽

...

Keyword(s):

Ovarian Cancer ◽

Ovarian Tumor ◽

Population Based ◽

Borderline Ovarian Tumor ◽

Low Grade ◽

Serous Ovarian Cancer ◽

Population Based Study

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Membrane type 1 matrix metalloproteinase proteolytic activity in initial adhesive and invasive events of ovarian cancer metastasis

10.32469/10355/48202 ◽

2014 ◽

Author(s):

◽

Lana Bruney

Keyword(s):

Ovarian Cancer ◽

Extracellular Matrix ◽

Proteolytic Activity ◽

Ovarian Tumor ◽

Molecular Mechanisms ◽

Cancer Metastasis ◽

Ectodomain Shedding ◽

Metastatic Process

Epithelial ovarian cancer (EOC) is one of the most common gynecologic malignancies, generally developing in women over the age of forty. When EOC are diagnosed prior to metastatic dissemination, the overall 5-year survival rate is 92%; however, nearly 85% of women with EOC are diagnosed with metastasis already present, dropping the survival rate to less than 30%. EOC, arises, arguably, from the single layer of cells that cover the ovary or fallopian tube. Metastatic ovarian tumors develop once an epithelial cell transforms, inducing detachment from the primary tumor site. These shed cells travel throughout the peritoneal cavity, escaping anoikis to survive as single cells and multicellular aggregates (MCA), and metastasize intraperitoneally through adhesion to and invasion of the mesothelial cell layer covering the peritoneum, the primary microenvironment for ovarian cancer metastasis. These mesothelial cells lie atop a collagen type I-rich extracellular matrix; subsequent to the initial attachment of ovarian cancer cells, proteolytic activity catalyzes migration through the mesothelial monolayer and promotes invasion of the sub-mesothelial matrix. Elucidating the early molecular mechanisms involved in this metastatic process, specifically the adhesion of EOC cells to mesothelial cells and penetration of the associated sub-mesothelial extracellular matrix, is essential to the development of future therapeutic agents. Enzymatic activity of matrix type 1 metalloproteinase (MT1-MMP), a transmembrane proteinase that degrades interstitial collagen, has been shown to be critical to this process. MT1-MMP activity has been directly implicated in both the invasion of the sub-mesothelial collagen I matrix, and in the shedding of metastatic MCA, but the molecular mechanisms behind these events are not completely understood. Considering the well-established role of MT1-MMP in the EOC metastatic process, identification of the molecules contributing to these pro-metastatic phenotypes is critical to future understanding of EOC metastatic spread. This research investigated the initial adhesive and invasive events of ovarian cancer metastasis, as associated with MT1-MMP proteolytic activity. Specifically, the effect of MT1-MMP activity on ovarian tumor cell ectodomain shedding and the in vitro, relationship between MT1-MMP and a potential phosphorylator, integrin linked kinase (ILK), on adhesion and invasion was assessed. Investigations utilized in vitro models of homotypic and heterotypic cell-cell adhesion, meso-mimetic invasion assays, and ex vivo tissue explants. Results suggest that ILK activity may catalyze phosphorylation of MT1-MMP to promote pro-metastatic events, including strengthening of adhesive contacts, invasion of the collagen-rich sub-mesothelial matrix, and MCA formation. Additionally, MT1-MMP expression may induce MUC16/CA-125 ectodomain shedding, which may then expose integrins at the ovarian tumor cell surface for high affinity cell-cell and cell-ECM binding.

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