TWIGS OF Andrographis paniculata (Burn. F) NEES ATTENUATES CARBON TETRACHLORIDE (CCl4 - ) INDUCED LIVER DAMAGE IN WISTAR ALBINO RATS

Carbon tetrachloride (CCl4) a pharmacological tool was used to induce liver damage in Wistar albino rats. Silymarin (100 mg/kg) and methanol extract of Lawsonia inermis Linn. family Lythraceae (known to possess hepatoprotective compounds) was used to reverse the liver damage caused due to CCl4 (induced toxicity). Hydroalcholic extract of stem bark and leaf of Lawsonia inermis Linn. was evaluated, for its restorative efficacy against CCl4 induced hepatotoxicity which was assessed in terms of biochemical and histopathological parameters. CCl4 produce the altitude levels of serum marker enzymes of alanine aminotransferase (ALT), asparate aminotransferase (AST), alanine phosphatase (ALP) and bilirubin in blood serum. Due to the toxicity of CCl4 cause turbine hepatic cell architecture, necrosis, inflammatory cell infiltration, congestion, and sinusoidal dilatation along with reduction of superoxide dismutase (SOD), catalase, Glutathione-S-transferase (GST) and glutathione peroxidase (GPx) appeared in liver tissue. SOD is the primary step in the defense mechanism involved in the antioxidant system against the oxidative stress. It diminishes by converting the superoxide radical in to peroxide and molecular oxygen. CAT or GPx reactions, also exert a similar effect thereby reducing the level of cellular damage. By oral administration of methanol extract of Lawsonia inermis Linn. plant extracts, i.e., stem bark extract (250 mg/kg b.wt.) and leaf extract (250 mg/kg b. wt.) the levels of these parameters was restored to near controlled (untreated) levels. Thus, the present study revealed that the extracts of stem bark and leaf of Lawsonia inermis Linn. offered protection against hepatotoxicity induced by CCl4.

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Effect of Methanol Extract of Abrus precatorius Leaves on Male Wistar Albino Rats Induced Liver Damage using Carbon Tetrachloride (CCl4)

Journal of Biological Sciences ◽

10.3923/jbs.2015.116.123 ◽

2015 ◽

Vol 15 (3) ◽

pp. 116-123 ◽

Cited By ~ 4

Author(s):

Uroko Robert Ikechukwu ◽

Sangodare Rose Simon Adeyi ◽

Muhammad Kabir Hadiza ◽

Asadu Chidimma Lilian

Keyword(s):

Carbon Tetrachloride ◽

Liver Damage ◽

Methanol Extract ◽

Albino Rats ◽

Abrus Precatorius ◽

Wistar Albino Rats

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Hepatoprotective effect of Averrhoa bilimbi Linn. methanol extract on carbon tetrachloride induced liver damage in albino rats

Planta Medica ◽

10.1055/s-0030-1264297 ◽

2010 ◽

Vol 76 (12) ◽

Author(s):

P Tupe ◽

D Nagmoti ◽

S Sakat ◽

A Juvekar

Keyword(s):

Carbon Tetrachloride ◽

Liver Damage ◽

Methanol Extract ◽

Hepatoprotective Effect ◽

Albino Rats

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The effect of baicalein on endoplasmic reticulum stress and autophagy on liver damage

Human & Experimental Toxicology ◽

10.1177/09603271211003634 ◽

2021 ◽

pp. 096032712110036

Author(s):

MC Üstüner ◽

C Tanrikut ◽

D Üstüner ◽

UK Kolaç ◽

Z Özdemir Köroğlu ◽

...

Keyword(s):

Endoplasmic Reticulum ◽

Transcription Factor ◽

Er Stress ◽

Liver Damage ◽

Albino Rats ◽

Tem Analysis ◽

Stress Markers ◽

Activating Transcription Factor 4 ◽

Activating Transcription Factor ◽

Wistar Albino Rats

Carbon tetrachloride (CCl4) is a toxic chemical that causes liver injury. CCl4 triggers endoplasmic reticulum (ER) stress and unfolded protein response (UPR). UPR triggers autophagy to deal with the damage. The aim of this study was to investigate the effect of baicalein, derived from Scutellaria baicalensis, on CCl4-induced liver damage concerning ER stress and autophagy. Two groups of Wistar albino rats (n = 7/groups) were treated with 0.2 ml/kg CCl4 for 10 days with and without baicalein. Histological and transmission electron microscopy (TEM) analysis, autophagy, and ER stress markers measurements were carried out to evaluate the effect of baicalein. Histological examinations showed that baicalein reduced liver damage. TEM analysis indicated that baicalein inhibited ER stress and triggered autophagy. CCl4-induced elevation of C/EBP homologous protein (CHOP), glucose-regulating protein 78 (GRP78), activating transcription factor 4 (ATF4), activating transcription factor 6 (ATF6), inositol requiring enzyme 1 (IRE1), pancreatic ER kinase (PERK), and active/spliced form of X-box-binding protein 1 (XBP1s) ER stress markers were decreased by baicalein. Baicalein also increased the autophagy-related 5 (ATG5), Beclin1, and Microtubule-associated protein 1A/1B-light chain 3-phosphatidylethanolamine-conjugated form (LC3-II) autophagy marker levels. In conclusion, baicalein reduced the CCl4-induced liver damage by inhibiting ER stress and the trigger of autophagy.

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Evaluation of anti-hepatotoxic potential of Cassia javanica (L.) methanol extract against paracetamol-induced liver damage in Wistar albino rats

Proceedings of the Mizoram Science Congress 2018 (MSC 2018) - Perspective and Trends in the Development of Science Education and Research ◽

10.2991/msc-18.2018.40 ◽

2018 ◽

Author(s):

Esther Lalhmingliani ◽

Zodinpuii ◽

R.K. Lalremtluangi ◽

Lalramdinthara Chenkual ◽

Lalthansangi

Keyword(s):

Liver Damage ◽

Methanol Extract ◽

Albino Rats ◽

Wistar Albino Rats

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Pharmacological and Pathological Studies on Taiwan Folk Medicine (IX): The Hepatoprotective Effect of the Methanolic Extract from Echinops Grijisii

The American Journal of Chinese Medicine ◽

10.1142/s0192415x93000054 ◽

1993 ◽

Vol 21 (01) ◽

pp. 33-44 ◽

Cited By ~ 8

Author(s):

Chun-Ching Lin ◽

Cheng-Hung Lin

Keyword(s):

Liver Biopsy ◽

Ethyl Acetate ◽

Liver Damage ◽

Methanol Extract ◽

Methanolic Extract ◽

Folk Medicine ◽

Hepatoprotective Activity ◽

Albino Rats ◽

Histopathological Changes ◽

Wistar Albino Rats

In order to isolate the main hepatoprotective component of Echinops grijisii, the crude drug was extracted with methanol and subjected to continuous extractions using n-hexane chloroform, ethyl acetate and n-butanol. The hepatoprotective studies of each fraction from the methanol extract of E. grijisii was conducted in Wistar albino rats with CC14-induced liver damage. Hepatoprotective activity was evaluated in terms of the modification of serum transaminase values such as SGOT and SGPT, and histopathological changes of liver biopsy. The results indicated that the main hepatoprotective component was concentrated in n-butanol and aqueous fractions.

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