Guanine and Guanosine Deaminase Activity of Rat Mammary Gland Homogenates Through Pregnancy and Lactation.

1957 ◽  
Vol 95 (1) ◽  
pp. 51-53
Author(s):  
W. F. Williams ◽  
C. W. Turner
Keyword(s):  
Mammary Gland ◽  
Pregnancy And Lactation ◽  
Rat Mammary Gland

β-Adrenergic Receptors in the Rat Mammary Gland during Pregnancy and Lactation: Characterization, Distribution, and Coupling to Adenylate Cyclase

Endocrinology ◽  
1990 ◽  
Vol 126 (1) ◽  
pp. 565-574 ◽  
Author(s):  
BIANCA MARCHETTI ◽  
MICHEL-A FORTIER ◽  
PATRICK POYET ◽  
NICOLLE FOLLEA ◽  
GEORGES PELLETIER ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Adenylate Cyclase ◽  
Adrenergic Receptors ◽  
Pregnancy And Lactation ◽  
Rat Mammary Gland ◽  
Β Adrenergic Receptors

scholarly journals Expression of Prolactin mRNA in Rat Mammary Gland During Pregnancy and Lactation

2000 ◽  
Vol 48 (3) ◽  
pp. 389-395 ◽  
Author(s):  
Toshiki Iwasaka ◽  
Shinobu Umemura ◽  
Kochi Kakimoto ◽  
Haruko Koizumi ◽  
Yoshiyuki R. Osamura
Keyword(s):  
Mammary Gland ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Late Pregnancy ◽  
Mammary Epithelial ◽  
Rt Pcr ◽  
Pregnancy And Lactation ◽  
Rat Mammary Gland ◽  
Pcr Method

We studied the expression of prolactin (PRL) mRNA in the mammary gland of resting, pregnant, lactating, and weanling rats using in situ and solution reverse transcriptase-polymerase chain reaction (RT-PCR). In mid- to late pregnancy and throughout lactation, PRL mRNA was detected in both in situ and solution RT-PCR. These PRL mRNA signals were clearly identified in the cytoplasm of alveolar and ductal mammary epithelial cells by the in situ RT-PCR method. In mid- to late pregnancy, such as at the initiating point of PRL mRNA expression, we confirmed in some cases a lack of PRL mRNA by solution RT-PCR. In addition, in the early weaning phase, no signals were detected by solution RT-PCR. However, slight focal signals were detected in some poorly vacuolated cytoplasm of regressing acinar cells by in situ RT-PCR. These findings suggest that PRL mRNA in rat mammary gland begins in mid- to late pregnancy in parallel with the development of the mammary gland, continues throughout lactation, and declines in the early phase of weaning, with regression of mammary epithelial cells.

Production of parathyroid hormone-related protein by the rat mammary gland in pregnancy and lactation

1992 ◽  
Vol 263 (6) ◽  
pp. E1077-E1085 ◽  
Author(s):  
M. Rakopoulos ◽  
S. J. Vargas ◽  
M. T. Gillespie ◽  
P. W. Ho ◽  
H. Diefenbach-Jagger ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Parathyroid Hormone ◽  
Mammary Tissue ◽  
Secretory Cells ◽  
Related Protein ◽  
Sprague Dawley ◽  
Parathyroid Hormone Related Protein ◽  
Pregnancy And Lactation ◽  
Rat Mammary Gland ◽  
In Pregnancy

Production of parathyroid hormone-related protein by the rat mammary gland in pregnancy and lactation. Am. J. Physiol. 263 (Endocrinol. Metab. 26): E1077-E1085, 1992.--Production of parathyroid hormone-related protein (PTHrP) by the mammary gland of Sprague-Dawley rats has been examined using immunohistochemistry and in situ hybridization to detect PTHrP and PTHrP mRNA, respectively. PTHrP and PTHrP mRNA could be demonstrated in nests of epithelial cells of the developing mammary gland at day 14 of pregnancy and in the epithelial secretory cells lining the alveoli during the latter stages of pregnancy and during lactation. A specific radioimmunoassay was also used to measure the concentration of PTHrP secreted in the milk throughout lactation. The concentration of PTHrP in milk was relatively low initially but increased during the latter stages of lactation, whereas calcium concentrations remained virtually constant throughout lactation. No correlation was found between the concentrations of calcium and PTHrP in rat milk. These results show that PTHrP is present in rat milk and also in mammary tissue before parturition, and therefore it may assist in the development of the mammary gland during pregnancy.

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