The effect of oral gavage (force-feeding) administration of fenugreek seeds (Trigonella foenum-graecum L.) on biochemical and neurobehavioural parameters in male Wistar rats

2021 ◽  
Vol 9 (1) ◽  
pp. 1-8
Faiza Rouag ◽  
Samir Djemli ◽  
Mabrouk Boussena ◽  
Rouya Memouni ◽  
Ines Refes ◽  
2017 ◽  
Vol 42 (8) ◽  
pp. 816-828 ◽  
Seetur R. Pradeep ◽  
Krishnapura Srinivasan

Oxidative stress has a crucial role in the progression of diabetes and its complications. Soluble fibre-rich fenugreek seeds (Trigonella foenum-graecum L.) are understood to have a beneficial effect in the management of diabetes. Previously, we have shown that the amelioration of diabetic hyperglycemia and related metabolic abnormalities was potentiated by onion (Allium cepa L.) in experimental rats. The present study evaluated the additive beneficial effect of dietary fenugreek seeds (10%) and onion (3%) on oxidative stress in diabetic rats. These dietary interventions lowered oxidative stress, the combination producing a higher beneficial effect (p < 0.05), although not additive. Dietary fenugreek, onion, or fenugreek+onion countered hypercholesterolemia (p < 0.05), especially from low-density lipoprotein−associated fraction by 43%, 35%, and 54%, respectively. Elevated concentrations of cholesterol and triglycerides in the liver and heart under diabetic conditions were significantly counteracted by these dietary interventions, with the additive combination resulting in greater effect. These findings were also corroborated by restoration of histopathological abnormalities of heart and liver tissues along with lowered heart and liver weights. It is inferred that an alleviation of oxidative stress contributes further to the antidiabetic influence and this nutraceutical potential of fenugreek seeds and onion was higher when consumed together.

2019 ◽  
Vol 16 (3) ◽  
pp. 0550
Iqbal Hasan AL-Khateeb

Separation of Trigonelline, the major alkaloid in fenugreek seeds, is difficult because the extract of these seeds usually contains Trigonelline, choline, mucilage, and steroidal saponins, in addition to some other substances. This study amis to isolate the quaternary ammonium alkaloid (Trigonelline) and choline from fenugreek seeds (Trigonella-foenum graecum L.) which have similar physiochemical properties by modifying of the classical method. Seeds were defatted and then extracted with methanol. The presence of alkaloids was detected by using Mayer's and Dragendorff's reagents. In this work, trigonilline was isolated with traces of choline by subsequent processes of purification using analytical and preparative TLC techniques. Further identification was done by using HPLC, IR and MP. Pure Trigonelline was isolated from the seeds of Trigonella-foenum graecum excluding other alkaloid like choline. In this study, a new, fast and convenient method for isolation and purification of Trigonelline from fenugreek seeds has been established. Unlike other methods, this one excludes all the non-alkaloidal components from the fenugreek seeds extract.

2012 ◽  
Vol 8 (2) ◽  
pp. 1555-1565 ◽  
Najla Hfaiedh ◽  
Sabah Dhibi ◽  
Sakria Mbarki ◽  
Jean-Claude Murat ◽  
Abdel Fattah Elfeki

Protective effects of Fenugreek seeds (Trigonella foenum graecum L), added in the diet, upon oxidative stress and dysfunctions in kidney, thyroid and liver of alloxan-diabetic rats were investigated.In our study, the alloxan-induced diabetes triggered 1) increased levels of glucose, total cholesterol and triglycerides in blood, 2) increased activities of alkaline phosphatase and transaminases in blood, 3) increased levels of creatinine, urea and protein in blood, 4) a decreased level of TSH and an increased level of free thyroxin in plasma.In addition, an oxidative stress, evidenced by an increase of lipids peroxidation level and superoxide dismutase activity associated with a decrease of glutathione peroxidase and catalase activities in hepatic and renal tissues, was observed.When Fenugreek seeds powder (100g/kg) was added in the food for 30 days, all this parameters were significantly shifted to more normal values.In conclusion, fenugreek seeds powder displays beneficial effects upon hepatotoxicity, nephropathy, thyroid dysfunction and oxidative stress in alloxan-diabetic rats. This property could be attributed to the presence of antioxidant components, such as complex polysaccharides and phenolic acids, as confirmed by analyses. 

2017 ◽  
Vol 2 (4) ◽  
pp. 184-188 ◽  
Fatemeh Yousef nasab ◽  
Mohammad Reza Hajinezhad ◽  
Hadi Hashemi ◽  
Abdolhossein Miri

Introduction: Trigonella foenum-graecum L. (Fenugreek) seeds and Cordia myxa fruits have been known as agents with anti-diabetes effects in Iranian traditional medicine. We compared the effects of aqueous extracts of these plants on diabetes-induced cognitive dysfunction. Methods: In our study, rats were equally allocated to 4 groups (n=12/group) as follows: control; diabetic; diabetic rats treated with T. foenum graecum seeds extract (TE) (870 mg/kg BW); and diabetic rats treated with C. myxa fruit extract (CE) (500 mg/kg BW) for 30 days. Diabetes was induced by alloxan (120 mg/kg BW, ip). Cognitive dysfunction was determined through memory task and passive avoidance learning (PAL). Results: Diabetes resulted in cognitive impairment in PAL and memory task. CE and TE significantly reduced serum blood sugar (P<0.05) and brain lipid peroxidation (P<0.001). Moreover, treatment with both extracts prohibited learning and memory deficits in diabetic groups in comparison to the non-treated group; however, there was no significant difference between the groups that were treated with TE or CE. Conclusion: Aqueous extract of fenugreek seeds and C. myxa are effective in preventing diabetesinduced lipid peroxidation and memory loss in animal models of diabetes.

Vijaykumar Kunvarji Parmar ◽  
Ketan Variya ◽  
Sandip Patel

ABSTRACTObjectives: The objective of this study was to investigate the acute toxicity of standardized protodioscin rich extract (PRE) of Trigonella foenumgraecumL (26.63% w/w; PRE).Methods: To evaluate the toxicity of PRE, the acute toxicity of the extract on adult rats were investigated. A fixed large dose of 2 g/kg body weight ofPRE was administrated by a single oral gavage, and 1 g/kg body weight of PRE was administered by intravenous according to the Organisation forEconomic Co-operation and Development guidelines.Results: In 2 weeks, PRE showed no obvious acute toxicity. There were no deaths in either group and no change in the clinical signs. The hematologicaland biochemical analyses showed some changes that returned to reference levels without impairment of homeostasis. The treatment did not induceuntoward changes in organs as shown by histological studies. The in vivo results showed that has low toxicity.Conclusion: PRE is safe and can be potentially used as an aphrodisiac in future studies.Keywords: Protodioscin, Aphrodisiac, Trigonella foenum-graecum, Organisation for Economic Co-operation and Development, Toxicity,Histopathological studies.

2020 ◽  
Vol 16 (5) ◽  
pp. 687-695 ◽  
Gullapalli Kowmudi ◽  
Krishnaveni Nagappan ◽  
Karthika Anoop ◽  
Mukkamala Sailaja ◽  
Narenderan S. T.

Background: Fenugreek seeds are employed in many traditional systems as an antibacterial, antidiabetic agent, gastric stimulant, and also for anti-invasive activity. Therefore, it is a suitable bioactive marker to establish the quality of crude drug and its formulations. Methods: A rapid, simple and sensitive LC-MS/MS analytical method was developed and validated for the determination of trigonelline extracted from Trigonella foenum-graecum (L.) (Fenugreek) and marketed dietary supplements using Etofylline as an internal standard. The objective of the present study is to quantify Trigonelline extracted from Trigonella foenum graecum L. (fenugreek) and marketed dietary supplements. Chromatographic separation was achieved on a Zorbax C18 column (50mm x 4.6mm i.d, 5μ particle size). The samples were eluted using 0.1% Formic acid in water: Methanol (20:80%v/v) at a flow rate of 0.5ml/min with a runtime of 5 min. The eluents were monitored using a tandem mass spectrometer equipped with an electro spray ionization source in positive mode. Results: The analysis was performed in multiple reaction monitoring (MRM) mode by quantifying the ion transitions from m/z 138.0→92.5 (Trigonelline) and m/z 225.0→180.90 (IS). The developed method was linear over the concentration range 5-50 ng/mL. The LOD and LOQ were found to be 1.0 ng/mL and 10.0 ng/mL, respectively. The correlation coefficient (r2) was found to be ≥0.998 for Trigonelline. Conclusion: The proposed validated LC-MS/MS method offers a sensitive quantification of trigonelline in Trigonella foenum graecum L. (fenugreek) and marketed dietary supplements containing fenugreek seeds.

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